Gene cloning and utility phophorylation assay of a protein-fused substrate for a highly sensitive detection of cdc2 protein kinase using a radioisotope detection technique for the development of a protein biochip

Ko, Kyong-Cheol,Choi, Mi Hee,Park, Sang Hyun John Wiley Sons, Ltd. 2009 Journal of labelled compounds & radiopharmaceutica Vol.52 No.4

<P>The prototype of the cdc2 protein kinase in mammalian cells regulates its entry into mitosis by phosphorylating a group of key proteins in the major cell cycle transitions. In this study, using the mep45 gene encoding the 45 kDa major envelope protein (Mep45) of Selenomonas ruminantium, a rumen bacteria, a Mep45-fused substrate (PKTPKKAKKL-Mep45, MFS-cdc2) was cloned to detect the activity of cdc2 protein kinase. We report here on a strategy for the detection of a phosphorylation of a substrate catalyzed by cdc2 protein kinase by using a radioisotope detection technique. It is possible to constantly obtain a reasonable quantity of MFS-cdc2 for the cdc2 protein kinase assay and its cost can be as low as a synthesized peptide. Results of the study indicate that the Mep45-fused protein can be used effectively as a substrate for detecting the activity of cdc2 protein kinase and it can be used in developing a protein biochip for a high-throughput screening and also for studying protein–protein interactions. Copyright © 2009 John Wiley & Sons, Ltd.</P> <B>Graphic Abstract</B> <P>Gene cloning and utility phophorylation assay of a protein-fused substrate for a highly sensitive detection of cdc2 protein kinase using a radioisotope detection technique for the development of a protein biochip Kyong-Cheol Ko, Mi Hee Choi, Sang Hyun Park<SUP>*</SUP>Using the mep45 gene encoding the 45 kDa major envelope protein (Mep45) of Selenomonas ruminantium, a rumen bacteria, a Mep45-fused substrate (PKTPKKAKKL-Mep45, MFS-cdc2) was cloned to detect the activity of cdc2 protein kinase. We report here on a strategy for the detection of a phosphorylation of a substrate catalyzed by cdc2 protein kinase by using a radioisotope detection technique. Copyright © 2009 John Wiley & Sons, Ltd. <img src='wiley_img/03624803-2009-52-4-JLCR1579-gra001.gif' alt='wiley_img/03624803-2009-52-4-JLCR1579-gra001'> </P>

읽기장애 아동의 음운처리 능력 특성 연구

김미경,서경희 한국특수교육문제연구소 2003 특수교육저널 : 이론과 실천 Vol.4 No.4

최근 읽기장애와 언어와의 관계의 중요성이 부각되면서 음운처리 결함이 읽기장애의 원인으로 널리 받아들여지고 있다. 이에 본 연구는 2학년, 4학년 일반아동 29명과 읽기장애아동 23명을 대상으로 음운인식능력 검사와 단어회상하기 검사, 문장회상하기 검사, 글자, 숫자, 사물, 색깔명명하기 검사를 통한 읽기장애아동들의 음운처리능력 특성을 연구하였다. 연구 결과 읽기장애아동들은 일반아동들에 비해 음운인식, 작업기억, 명명속도에서 유의미한 차이를 보였다. 이러한 결과를 바탕으로 일반학급과 특수학급에서 읽기장애 아동들을 지도하는 프로그램개발에 기여할 것을 제안한다. The purpose of this study was to investigate difference between normal children and children with reading disabilities, ranged from grade 2 to grade 4 in phonological processing ability. The sample was classified into two groups: (1) a group of 23 children with reading disabilities; (2) a group of 29 normal children. Students in the sample of this study do not have severe emotional problems, uncorrected vision problems, hearing loss, acquired neurological disorders. The results of this study were as follows: 1.Children with reading disabilities performed significantly poorly on test of phonological awareness than children without reading disabilities. 2.Children with reading disabilities performed significantly poorly on test of working memory than children without reading disabilities. 3.Children with reading disabilities performed significantly poorly on test of naming speed than children without reading disabilities. There is also interaction effect between the presence or absence of reading disability and grade on naming speed.

증거기반 평가 설계와 타당도의 통합적 관점에서의 문항 특성 분석 활용

송미영,전경희 이화여자대학교 교육과학연구소 2022 교육과학연구 Vol.53 No.3

본 연구는 학생 평가의 타당화를 위해 증거기반 평가 설계와 타당도의 통합적 관점에서 문항 특성 분석을 활용하는 방안을 탐색하는 데 목적이 있다. 이를 위해 타당도의 고전적 개념을 확장한 통합적 접근을 살펴보고, 문항 특성 분석 기법을 활용하여 평가 계획에서부터 평가 도구 개발, 평가 결과의 분석 및 활용에 이르기까지 학생 평가의 전반적인 운영 과정에서 타당도를 제고하는 방안을 제안하였다. 구체적으로 타당도의 증거 유형별 문항 특성 분석 적용 방안을 제시한 후, 이를 경험적 자료에 적용하여 방법론적 적합성과 유용성을 검증하였다. 이어 학생 평가의 타당도 제고를 위해 증거기반 평가 설계 모형과 문항 특성 분석을 연계하여 활용하는 방안을 탐색하였다. 실제 교육과정 기반의 대규모 평가 자료에 문항 특성 분석을 적용한 결과, 이러한 접근 방법이 문항 특성에 대한 포괄적이고 심층적인 정보를 제공함으로써 학생 평가의 타당도 증거를 강화하기 위한 유용한 분석틀이 될 수 있음을 시사하였다. 이를 통해 문항 특성 분석이 국가 수준 및 시도교육청 단위의 대규모 평가뿐 아니라 단위 학교 평가 맥락에서의 학생 평가 타당화 과정에서 다양하고 종합적인 증거를 수집하는 데 확장성이 큰 방법론으로 활용될 수 있음을 확인하였으며, 이에 따른 교육적 시사점을 논의하고 후속 연구를 제안하였다. This study explored feature analysis (FA) from the perspective of an integrated approach to validity and evidence-centered design. To this end, this study aimed to demonstrate how FA provides validity evidence in the overall operation process of student assessments based on an integrated approach that extends the classical concept of validity. Specifically, the methodological suitability and usefulness of FA were verified by applying the approach to empirical assessment data and presenting data collection procedures for each type of evidence of validity. Next, from the perspective of enhancing the validity of student assessment, ways of linking evidence-centered design with FA were suggested, and methods for applying FA to each stage of the evidence-centered design were explored. The results of applying FA to the 2019 National Assessment of Educational Achievement indicated that FA can be a functional analytic framework for enhancing validity evidence of student assessments by providing comprehensive and in-depth information on item features. Hence, the results of this study suggest that FA can be extended to a highly elaborate approach to collect various and comprehensive evidence in the process of validating student assessments in the context of individual school assessment as well as large-scale assessment at the national and provincial level. Based on such results, educational implications and suggestions for follow-up studies were discussed.

(Re)Imagining Korea:A Beginner’s Guide to Contemporary Korea : Understanding Contemporary Korean Culture (Seoul:Jimoondang, 2011)

권경미 이화여자대학교 한국문화연구원 2012 한국문화연구 Vol.22 No.-

정신분열병 환자의 인터류킨-2 생산능

김경용,김임,박주흥,은헌정,김동인,이선미 예수병원 1997 예수병원학술지 Vol.18 No.-

일부농촌지역의 농약사용실태 및 농약중독 요인 조사

이경민,송주희,장재혁,심수정,강양화,안재경,이숭호,박미영,정문호 대한보건협회 2002 대한보건연구 Vol.28 No.4

Object The usage of agricultural chemical is on the increasing. Use of pesticides has increased agricultural production. However, negative externalities from such use have increased too. Pesticide poisoning is a major public problem. The purpose if this study is to find out the relationship between keeping the safety rules or protective equipments or attending safety education or dose of pesticides use , and poisoning experience. Methods For Gu, H.M. and Pack, S.G. study , we obtained questionnaire data concerning pesticides poisoming. A person interviews on 203 were conducted in two villages in Sinmeari and woulbonri Chunchun , Kangwon province, July 2002. Result 1. 144 people(70.9%) have sprayed pesticides and the rata of experiences of pesticide poisoning was 26% among 144. Spraying time of pesticides was 1-2 hours in 63.9% of farmers. Rate of using the protective equipment was 25.7% (protective clothes), 49.3% (Mask), 48.6 (Gloves), 7.0 (Protective glasses), 64.6% (boots) respectively. 2. Chi-square and multiple logistic regression analysis showed that Duration of spraying per day (<2hr) and using of mask(protective equipment)are significantly association with poisoning experience.(x^2=5.2684 p=0.0217, CI=0.140-0.5853 OR=0.346 p=0.0211 respectively). But no association between poisoning experience and keeping safety rule, attending of safety education. 3. Spraying pesticides. Duration of spraying pesticides, reading manuals and no spraying at fatigue (keeping safety rule) are significantly association with symptom experience(x^2=14.0621 p=0.0002, x^2=7.0639 p=0.0079, CI01.395-10.950 OR=3.908 p=0.0095, CI=0.101-0.726 OR=0.271 0.0094 respectively). But no association between symptom experience and protective equipments, attending of safety education.

가덕도주변 해역 해수의 중금속 함량

노미경,차미선,박근태,손홍주,문두호,이상준 부산대학교 환경문제연구소 2000 環境硏究報 Vol.18 No.-

The quantitative analysis of heavy metals(As, Cd, Cr, Hg, Pb, Cu) in the sea water around Kaduck island was conducted during the period of March, 1998 to September, 1998. The sea water in the four regions was sampled four times and the heavy metals were analyzed. The Heavy metal concentration of the sea water around Kaduck island was As 127.7㎍/ℓ , Cd 49.28㎍/ℓ , Cr 0.462㎍/ℓ, Hg 0.111㎍/ℓ, Pb 1.713㎍/ℓ, Cu 2.716㎍/ℓ in the average value, respectively.

Electrochemical synthesis of nanosized hydroxyapatite/graphene composite powder

Vesna Mišković-Stanković,Sanja Eraković,Ana Janković,Maja Vukašinović-Sekulić,Miodrag Mitrić,Young Chan Jung,Soo Jin Park,Kyong Yop Rhee 한국탄소학회 2015 Carbon Letters Vol.16 No.4

Electrochemical synthesis was employed to prepare a novel hydroxyapatite/graphene (HAP/ Gr) composite powder suitable for medical applications as a hard tissue implant (scaffold). The synthesis was performed in a homogeneous dispersion containing Na2H2EDTA·2H2O, NaH2PO4 and CaCl2 with a Ca/EDTA/PO4 3− concentration ratio of 0.25/0.25/0.15M, along with 0.01 wt% added graphene nanosheets, at a current density of 137 mA cm−2 and pH value of 9.0. The field emission scanning electron microscopy and transmission electron microscopy observations of the composite HAP/Gr powder indicated that nanosized hydroxyapatite particles were uniformly placed in the graphene overlay. Raman spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction confirmed graphene incorporation in the HAP/Gr powder. The electrochemically prepared HAP/Gr composite powder exhibited slight antibacterial effect against the growth of the bacterial strain Staphylococcus aureus.

정신분열병 환자의 인터류킨-2 생산능

이선미,박주홍,김동인,은헌정,김임,김경용 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.4

급성기 및 관해상태의 정신분열병 환자와 정상 대조군의 IL-2 생산능을 비교함으로써, 정신분열병의 여러 발병원인 중 자가면역 병인론의 증거인 세포성 면역방응의 저하 및 질병상태와 관련한 세포성 면역기능의 변화를 일부 한국인 정신분열병 환자에서 조사에서 조사하고자 하였다. 방 법 : DSM-IV의 정신분열병 진단기준에 적합한 급성기 환자 19명(남 : 10, 여 : 9)과 관해상태의 환자 21명(남 :12, 여: 9) 및 정상대조군 40명(남 : 20, 여 : 20)을 연구대상으로 하였다. 피험자로부터 채혈한 혈액에서 임파구를 분리하여 phytohemagglutinin으로 T임파구를 자극하고 세포배양하여 증식시킨후 IL-2immunoassay kit을 이용하여 T임파구의 IL-2 생산능을 측정하였다. 결 과 : IL-2 생산능은 성별에 관계없이 정신분열병 환자군에서 정상 대조군에 비하여 유의하게 감소하였다. (t=5. 97, p<0.001). 질병상태에 따라 급성기 정신분열병 환자군 및 관해상태 정신분열병 환자군으로 구분하여 정상대조군의 IL-2 생산능과 비교한 결과, IL-2 생산능이 정상 대조군에서 가장 높았고 다음은 관해상태 환자군이었으며 급성기 환자군에서 가장 낮았다. 세 군 사이에는 모두 유의한 차이가 있었다. (F=35.35, p<0.001). 결 론: 일부 한국인 정신분열병 환자에서 질병상태와 유의하게 관련된 세포성 면역기능의 저하가 관찰되며, 이는 정신분열병의 다양한 원인중 자가면역 기전에 의한 발병을 시사한다. Objects : This study was designed to examine the decreased cellular immune response and the change in cellular immunity related to to clinical status in Korean schizophrenic patients. Methods : The subjects were 19 acute schizophrenics and 21 remitted schizophrenics who met the DSM-IV criteria of schizophrenia and 40 healthy volunteers as the normal control. After the lymphocyte was separated from blood which had been drawn from the subjects and then T lymphocyte was stimulated by PHA and proliferated by cell culture, the examiner measured IL-2 productivity by IL-2 immunoassay kit. Results : IL-2 productivity of the schizophrenics was significantly lower than that of the normal controls. There was no significant difference between male and female in two groups. In the comparison by clinical status, acute schizophrenic group was the lowest, remitted schizophrenic group was next, and normal control group was the highest in the IL-2 productivity, with statistically significant difference among the groups(p<0.001). Conclusion : We suggest that there is a decrease in the cellular immune response which is significantly related to clinical status in some Korean schizophrenics. This finding supports that onset of schizophrenia may be the result of autoimmune mechanism, which is one of the various etiologic factors in schizophrenia.

치위생사의 건강보험 요양급여기준과 치과진료수가기준에 대한 인지도

전미용,박경숙 중앙대학교 의과대학 간호학과 간호과학연구소 2000 중앙간호논문집 Vol.4 No.2

Korean health insurance finally established the whole national health insurance by its rapid development since the health insurance was conducted 12 years ago, and owing to the fact the meidcal usage of the people was suddenly increased. Consequently, in this study I tried to understand the degree of the knowledge after researching the degree of the recognition of the health insurance of dental sanitarians who worked in the dental meidcal organization, and to suggest the basic data about the importance of the future health insurance education and the effective educational program, I abstracted some people arbitrarily of 300 sanitarians who worked in dental clinics and dental medical centers located in Seoul from October 9th to October 28th 2000, and the I surveyed them with direct interviews, and finally I performed the statistical anaysis treatment on 228 people of those sanitarians above. The collected data were analyzed using SAS(Statistical Analysis System) program, and the conclusion I could find through the study is as follows. 1. The degree of the knowledge about the recuperation payment of the dental sanitarian was 3.07 as an average score, and in descending order they can be displayed as the outside-hospital prescription, recuperation payment process, and the objection request for the treatment fee, and on the other hand, the lowest one was the computation of the recuperation payment period. 2. The degree of the knowldege about the standard of the dental treatment cost fee of the dental sanitarian was 3.44 as an average score, and in descending order they can be displayed as the rapid daily charging treatment, muscular tube medical care, and the mouth surgery in the field of the dental disease treatment, and in ascending order the lower articles were the organ regeneration inducement treatment, bone organs deformity correcting treatment, and the dental muscular excision treatment in the field of the surgeon. 3. The degree of the knowldege in each general characteristic of the dental sanitarians was higher in those who were old enough and had a lot of clinical experiences and there was a lot of cases that the sanitarians themselves requested in the electronic documentary interchange way about the standard of the recuperation payment and the standard of the dental treatment cost fee. When summarizing those results above, in order to increase the degree of the knowledge about the health insurance of the sanitarians, the consistent and systematic payment education should be performed. Especially, because in the group which was consisted of people who were insufficiently aged and had unsatisfactory clinical experiences, the degree of knowledge about the health insurance was shown to be low, the work guid book about the recruit dental sanitarian should be prepared, and the systematic payment education and information service are thought to be necessary. Additionally, when educating about the payment to the dental sanitarian, the payment eudcation about the concrete contents such as the dental treatment and the calculation guideline for the remedy fee, medication prescription, and medicine compounding fee should be performed. The health insurance cost fee is bing changed continuously, and the payment education of the dental sanitarians should be performed continuously, and additionally because there is no insurance-inspecting dental sanitarian who charges the inspection work professionally, the cultivation of the specially trained employees and the institutional backup for increasing the participation degree in the payment education should be executed.