Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

TNT 분해세균 Pseudomonas sp. HK-6에서 정제한 NAD(P)H-nitroreductase의 특성연구

호은미,오계현 순천향대학교 기초과학연구소 2003 순천향자연과학연구 논문집 Vol.9 No.2

The aim of this work was to characterize NAD(P)H-nitroreductase purified from Pseudomonas sp. HK-6, which could utilize 2,4,6-trinitrotoluene. In initial experiments, NAD(P)H-nitroreductase from actively growing HK-6 cells was separated by ammonium sulfate precipitation, DEAE-sepharose, and Q-sepharose. Specific activity of NAD(P)H-nitroreductase was approximately 4.46 unit/㎎. Optimum temperature and pH of the enzyme were approximately 30℃ and 7.5, respectively. Specific activity of NAD(P)H-nitroreductase on the effect of several metal ions was monitored. Fe^(3+) showed stimulatory effect on the activity of NAD(P)H-nitroreductase, whereas the specific activity of the enzyme was determined to approximately 27 kDa by SDS-PAGE.

SMEDDS를 이용한 난용성 약물의 용출율 향상

김계현,이윤석,배준호,지상철,박은석 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

ABSTRACT-A self-microemulsifying drug delivery system (SMEDDS) was developed to enhance the solubility and dissolution rate of poorly water soluble drug, biphenyl dimethyl dicarboxylate, DDB. The system was optimized by evaluating the solubility of DDB and the microemulsion existence range after the preparation of microemulsions with varying compositions of triacetin and surfactant-cosurfactant mixtures (Labrasol as surfactant (S) and the combination of Transcutol, Cremophor RH 40 and Plurol oleique as cosurfactant (CoS)). SMEDDS in this study markedly improved the solubility of DDB in water up to 10 mg/ml and the size of the o/w microemulsion droplets measured by dynamic light scattering showed a narrow monodisperse size distribution with an average diameter less than 50nm. The microemulsion existing range is increased proportional to the ration of S/CoS, however, it decreased remarkably as the oil content was more than 20%. In vitro dissolution study of SMEDDS showed a significantly increased dissolution rate of DDB in water (>12 fold over DDB powder), and SMEDDS also had significantly greater permeability of DDB in Caco-2 cell compared to powders.

목표계획법을 이용한 사단급 ASL 선정모형에 관한 연구

길계호,김충영 한국경영과학회 1998 한국경영과학회 학술대회논문집 Vol.- No.2

The selection criteria of the Authorized Stockage List (ASL) in the Army is based on Army Regulation (AR) 409, the selection method of ASL is not considered in cost, weight and volume of repair parts. This paper is focused on developing for a new selection model taking account of cost, weight and volume of repair parts. This model is applied to data of a division. The ASK selected in the model is more reduced in cost, weight and volume than that of the previous method.

성인 양극성장애와 정신분열병 환자의 아동기 주의력결핍 : 과다활동 증상

신정호,안정숙,이계성 대한소아ㆍ청소년정신의학회 1998 소아청소년정신의학 Vol.9 No.1

변형된 Radial 기반 신경회로망을 이용한 다중 물체 검출 및 세그멘테이션

金柱慶,全俊亨,金鎭浩 慶一大學校 1996 論文集 Vol.13 No.2

본 논문에서는 변형된 RBF 신경회로망을 이용하여 다중 물체에 대한 사전 정보 없이도 효과적으로 다중 물체를 검출 및 분리할 수 있는 알고리즘을 제안하였다. 제안된 알고리즘에서는 대칭성을 잘 반영하는 RBF 신경회로망으로 GST(generalized symmetry transform)를 구현하기 위해 서로 대칭인 위치에 존재하는 화소들이 에지 방향성분 차이를 MRBNN의 입력으로 하고, 대칭인 화소간 거리의 함수로 가중치를 설정함으로써 대칭성이 충분히 반영될 수 있도록 하였다. 또한, 입력영상에 대한 경계선 추출 영상을 피라미드 구조로 계층화 한 후, 피라미드 구조의 각 단계에 MRBNN을 적용함으로서 고정된 크기의 대칭 마스크로도 물체 검출 및 분리가 동시에 가능하도록 하였다 여러 물체가 혼재하는 실영상에 대해 실험해 본 결과 물체의 중심 추출을 통한 물체검출 및 물체 분리가 효과적으로 이루어짐을 확인할 수 있었다.

호텔 조리부 베이커리 조리사의 이직요인에 관한 연구

민계홍,안호기 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.2

The purpose of this study was to examine the causes of bakery employees quitting their jobs at hotels in order to make more efficient use of human resources by increasing personnel management efficiency and reducing the turnover rate, The subjects in this study were employees working for bakeries at top-ranked hotels, and the responses from 266 respondents were selected for analysis. The survey was conducted from July 1 through 20, 2003. Frequency analysis, factor analysis, reliability analysis, t-test, ANOVA and regression analysis were performed for data handling. The major findings of this study were as follows: First, concerning salaries, their turnover intention was influenced by whether they were properly paid or gained a sufficient living. Second, as for environmental factors, their turnover intention was affected by performance appraisal and interpersonal trust with colleagues. Third, regarding job-related factors, their turnover intention was impacted by the clarity of their job definition, their degree of job satisfaction and the suitability of their posts. Fourth, the change of job was influenced by the desire for a job in another region, in another company and of another kind. To successfully manage employee turnover at hotel bakeries, employees should be properly paid, and different performance appraisal standards, including a multilateral evaluation system, should be applied according to the nature of the department. Furthermore, there should be a clear distinction between sales and other works, and they should be carefully treated to increase job satisfaction.

한국 프로야구 선수들이 지각한 감독의 리더십과 선수들의 스트레스 및 생활 만족도에 관한 연구

이상호,이계윤,김갑룡,원유병,이해권 창원대학교 체육과학연구소 2002 경남 체육연구 Vol.7 No.1

현장에 적용하여, 한국 프로야구 감독자들의 리더 역할이 효율적 또는 비효율적 프로파일 중에서 어느 프로파일에 해당하는지 알아보고, 그리고 감독자들의 6개 리더 역할과 선수들의 스트레스 및 생활만족도 간에 상관 관계를 분석하였다. 조사대상은 해태 야구 선수를 포함하여 해태 야구 선수들과 시합을 하기 위해 어웨이 경기차 광주를 방문한 7개 구단 선수들을 본 연구자가 직접 숙소로 찾아가 조사 목적과 설문 내용을 설명하고, 본 설문지에 응답토록 하였다. 조사된 설문지 중에서 성의없이 응답했거나, 전체 문항중 10%이상 응답을 하지 않은 자료에 대해서는 연구 대상에서 제외한 결과 최종 145명을 연구자료로 확정하였다. 측정도구는 하형주(1996)가 개발한 스포츠 지도자의 대응가치 리더십 검사지(CVLQS:Competing V alues Leadership Questionnaire for Scale)를 사용하였다. 스트레스를 알아보기 위해 첫째, 한국 스트레스 매니지먼트 연구소에서 개발한 심리적, 신체적 스트레스 척도(1990)를 사용하였고, 둘째, 운동 스트레스는 이계윤(1997)이 개발한 프로야구 선수들의 스트레스 척도를 사용하였다. 자료분석은 먼저 선행 연구에서 밝힌 스포츠 지도자의 효율적인 리더십 프로파일을 제시한 후, 각 구단별 프로야구 감독들의 리더 역할에 대해 Z점수를 구하고, Quinn(1988)이제안한 리더십 프로파일에 적용하여 비교 분석하였다. 마지막으로 한국 프로야구 감독들의 6개 리더 역할과 선수들의 스트레스, 생활 만족도 간에 상관 분석을 하였다. 이상과 같은 연구과정을 통해 도출된 결론은 다음과 같다. 첫째, 효율적인 리더역할 프로파일에 해당한 감독은 8개 구단 감독 중 감독 2, 3, 4, 7, 8로 나타났고, 비효율적인 리더역할 프로파일에 해당한 감독은 감독 1, 5, 6으로 나타났다. 둘째, 한국 프로야구 감독들의 리더역할과 운동선수들의 스트레스, 생활만족도 간의 상관분석은 감독의 리더역할과 운동선수들의 신체적 스트레스, 운동 스트레스, 생활만족도 간에 유의한 상관으로 나타났다.

인도네시아 연구기관의 인적자원관리 시스템 개발을 위한 목표모형

송계충,조영호,이광순 충남대학교 경상대학부설 경영경제연구소 1996 경영논집 Vol.12 No.2

재래시장에서의 접근성과 업종별 상관관계 : 광주광역시 대인시장과 남광주시장을 중심으로 Focused on Daein and Namgwangju markets in Gwangju

정금호,이계순,신남수 대한건축학회 2004 대한건축학회논문집 Vol.20 No.1

The conventional market places are the behavioral space by physical environment and human activity. We discuss ways and means of reviatalizing conventional market. Focused on consumer's aspect and physical improvement of building and equipment. In spite of it's small size, small area. Market is directly connected with daily life of citizen and plays a major role for urban activities and systems. Therefore is necessary to study the physical characteristics related to human activity for improving the physical environment of conventional market. This study base on an assumption that location of industrial classification depend on accessibility and relation with street. The purpose of this study is to analyze relation between street and industrial classification in conventional markets. For the logistic analysis, We investigated type of business, general road width, public land price, connection value, distance from a bus stop, distance between main street and the each store in Daein and Namgwangju markets. The statistical analysis says that 1. In the markets, food stores are located near from the main streets. 2. Clothing stores are located near from the main streets but far from the bus stops. 3. The culture type of business is located far from the main streets. 4. The neighboring facilities are located on the narrow roads and at the lands high in the public price.