난소절제가 흰쥐 대동맥의 미세구조에 미치는 영향

최용귀,전영희,백태경,백두진,정호삼 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.2

한국인의 치아우식 조절에 관한 연구 : Ⅱ. Streptococcus mutans strain B-13이 생산한 불용성 세포외 다당류와 dextransucrase 활성 Ⅱ. INSOLUBLE EXTRACELLULAR POLYSACCHARIDES AND ACTIVITY OF DEXTRANSUCRASE PRODUCED BY STREPTOCOCCUS MUTANS STRAIN B-13, IN VITRO

정태영,정동균,이효재,김각균,오귀옥,이종흔 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1

Insoluble extracellular polysaccharides synthesized by Streptococcus mutans type d strain B-13 were analysed in an effort to elucidate their chemical composition. Dextransucrase produced in supernatant was also studied for its activity. 1. The microorganism produced 2.10g of total insoluble fraction which contained 1.59g of insoluble polysaccharide and 0.41g of bacterial mass per 800ml of culture medium. 2. Insoluble fraction contained 26.1㎍/mg of nitrogen and 746.4㎍/mg of insoluble extracellular polysaccharides. Ketohexose content of polysaccharides was 7.12%. 3. Streptococcus mutans strain B-13 was capable of producing 4.10g insoluble extracellular polysaccharide per 1.0g of bacterial mass. 4. Dextransucrase liberated 5.11mg of hexose per 1ml of culture supernatant.

Agrobacterium tumefaciens를 이용한 amorpha-4,11-dience 합성 유전자의 상추로의 도입

정귀미,송명종,백남인,박영두 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

Amorpha-4,11-diene synthase(ADS) 유전자를 상추에 도입하기 위해 ADS유전자를 pILTAB357에 삽입하여 binary vector pADS를 제작하였다. 상추 형질전환은 자엽 절편체를 pADS 벡터를 운반하는 A. tumefaciens LBA4404에 접종시키고 MS배지에 200 mg·L 세파탁심과 50 mg·L 가나마이신이 첨가된 선발 재분화배지에서 선발하였다. 선발된 신초는 뿌리 유기 배지에서 뿌리를 유기한 후 뿌리가 발생한 신초는 토양으로 이식하여 재배하였다. 형질전환상추에 T-DNA가 전이되었는지를 확인하기 위해 genomic DNA를 이용하여 PCR과 Southern 혼성화 분석을 실시하였다. PCR 분석 결과 9개의 재분화 개체로부터 기대했던 1.6 kb의 산물을 확인할 수 있었으며 Southern 혼성화 분석 역시 각 재분화 개체에 ADS 유전자가 1개 내지 3개의 copy수로 전이된 것을 확인할 수 있었다. 형질전환 상추내의 artemisinin 함량을 GC와 GC/MS 방법으로 분석하였다. Artemisinin 표준물은 retention time이 10분 7초로 나타났으며 이 peak가 artemisinin인지 확인하기 위하여 GC/MS를 측정한 결과 m/z 282가 정확하게 나타나 artemisinin으로 확인되었다. 한편 형질전환 상추와 일반 대조구 상추의 시료도 GC chromatogram에서 artemisinin의 retention time인 10분 7초 부근에서 여러 peak이 겹쳐서 나타났으나 각 peak에 대하여 GC/MS를 측정한 결과 m/z 282을 나타내는 peak는 관측되지 않아 형질전환 상추와 일반 상추에는 artemisinin이 함유되어 있지 않은 것으로 확인되었다. To introduce amorpha-4,11-diene synthase (ADS) gene into lettuce plant, binary vector pADS was constructed by the insertion of an ADS gene to pILTAB357. Cotyledon explants were infected by A. tumefaciens LBA4404 carrying pADS vector to transform lettuce plant with ADS gene. Transformed shoots were selected on selective regeneration medium containing MS salts, 200 mg·L cefotaxim, and 50 mg·L kanamycin. The selected shoots were transferred to rooting medium, and regenerated shoots were transplanted to pots after roots appeared. PCR and Southern hybridization analysis of genomic DNA were performed to confirm expected T-DNA fragments in the transgenic lettuce plants. PCR analysis revealed the presence of the expected 1.6 kb product from the nine regenerants. Southern hybridization analysis also showed that ADS gene was transferred into transgenic lettuce plants with 1~3 copies. Artemisinin in lettuce was identified by GC and GC/MS analysis. Standard artemisinin was found at 10 min 7 sec in the retention time by GC analysis and confirmed m/z 282 by GC/MS. There were several peaks at retention time of both non-transformed and transformed lettuce plants. However, GC/MS analysis showed that peak with m/z 282 was not found in transgenic lettuce plants.

규칙적인 운동프로그램 참여가 중년여성의 정신건강에 미치는 영향

김영구,윤귀현 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

The purpose of this study was to examine the effects of Regular Sports Program of middle aged woman on mental health of the middle aged women A total of 249 subjects (participants of Regular Sports Program in Seoul : 122, non-participants of recreational sports activity: 127 were randomly selected. SCL-90-R was conducted to test the difference In mental health between participants and non-participants, The results were as follows; Among nine factors of the index of mental health indicated that only somatization and anxiety were significantly different between participants and non-participants. The results of analyese by age-group showed that phobic anxiety was significantly different among 30s by the participation of recreational sports acitivity. As a result of nine factors of mental health by age-group analysis, sensitivity, anxiety, hostility, phobic anxiety and paranoid ideation were significantly different among the participants of recreational sports activity. In the case of non-participants, somatization, quick sense of personal relations, depression, anxiety, hostility, phobic anxiety and paranoid ideation were significantly different.

망간 노출 근로자의 정신 ·신경행동 양상에 영향을 주는 요인

문영한,권기련,김은아,김규상,김양호,김재우,노재훈,양정선,진영우 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.2

The risk posed to human health by environmental manganese (Mn) exposure is unknown. The purpose of this study is to establish if subclinical effects related to Mn exposure and examine the factors influencing psychoneurobehaviral outcomes of Mn exposed workers. This study involved 121 male workers of welding, alloy furnace, and manufacturing of welding stick. Study investigations include: a questionnaire covering demographic characteristics, job and exposure history and medical symptoms and conditions, monitoring workplace air for personal exposure to respirable and inhalable manganese, analysis of blood and urine samples, psychoneurobehaviral test(neurobehaviral core test battery(NCTB), signal change on magnetic resonance imaging (MRI) scans, minimental state and neurological examination). The main results of this study were as follows; 1. Preliminary results showed that, with increasing manganese exposure, neurobehaviral performance was poorer and signal change on MRI scans increased. 2. The results of examination revealed significant relations on psychoneurobehaviral outcomes (neurobehaviral performance, signal change on MRI scans, and neurological features). 3. In multiple regression, age and educational status as demographic variables and exposure level were significantly related to neurobehaviral test. Also exposure level and serum Mn concentrations were positively related to signal change on MRI scans and neurological features affected by Mn on the brain (signal change). 4. Psychoneurobehaviral outcomes from Mn exposure were related to neurobehaviral performance, signal change on MRI scans, neurological features and profile of mood states (POMS), and influenced positively with age, alcohol and smoking history, and duration of Mn exposure, negatively with educational status. Serum Mn concentrations in combination with brain MRI scans, and perhaps a battery of neurobehaviral tests, appear to be the best way to monitor excessive exposure to Mn. These results are consistent with our knowledge on Mn action on the brain and are similar to the type of neurobehaviral dysfunction. They suggest that there may be age, educational status, and life style (alcohol and drinking history) differences with chronic environmental exposure. These findings suggest further evaluation, particularly on relationships between Mn exposure, aging, and susceptibility factors.

고유 불안정한 도립진자 시스템의 최적 안정화 제어에 관한 연구

최부귀,박영식,안기형,이취중 동아대학교 공과대학 부설 한국자원개발연구소 1990 硏究報告 Vol.14 No.1

This paper proposes a design techinque of dynamic stabilization controller for the intrinsic unstable inverted pendulum system. Mathematical modelling with the more complex nonlineaity and the stabilized control theory presented by C.D. Johnson are adapted to this system by using the stage-space approach. And the stabilized controller with the designed optimal regulator type which can be fastly tracked and can be accurately counteracted against all effects of the constant disturbances and the parameteric variations is sumulated and is implemented successfully on the microcomputer.

將來世代에 대한 環境保護義務의 正當化 : A. de-Shalit에 의한 世代間正義의 共同體論的 어프로치를 중심으로

高眞融男,河喆永,韓貴鉉(譯) 동의대학교 법정연구소 2001 공공정책연구 Vol.17 No.-

대장게실염 -18 예-

김광연,차귀현,이영희,유경수 대한대장항문학회 1986 Annals of Coloproctolgy Vol.2 No.1

한국인의 치아우식 조절에 관한 연구 : Ⅲ. 白鼠우치痍患率에 미치는 數種 稀 有元素, 쌀 및 各種 양념의 影響 Ⅲ.EFFECTS OF SEVERAL TRACE ELEMENTS, RICE AND SPICES ON THE CARIES RATE IN RATS

鄭東均,鄭素英,李孝宰,金各均,吳貴玉,李種昕 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1

Two-part experiment was conducted to elucidate the effects of several trace elements, rice and spices on caries rate in Korean albino rats. Rats were selected into two large groups according to their weight ; one group (for part I experiment) consists of rats weighing 140-200gm and the other group (for part II experiment) consists of rats weighing 76-109gm, and the same experiment was conducted independently for both groups. Rats were fed standard cariogenic diet supplemented with rice (21, 53%), capsicum (1, 10%), garlic (0.5, 5 or 10%), phosphorus (2%), sodium chloride (3%, reagent grade), or table salt. Drinking water was tap water containing cold water extract of capsicum (0.1%) alcoholic extract of capsicum (0.1%), water extract of ginseng (2%), fluoride (10, 50 or 100ppm) or 50ppm of Mg, Zn, Mo, Sr, Mn of Fe. The results were as follows. 1. 24.9%, 51.0% and 54.3% decrease in caries score were recorded for rats which drank water containing 10, 50 and 100ppm of fluoride in part I experiment. In part II experiment caries score decreases 51.8% and 56.9% for 10ppm and 50ppm fluoride content. 2. Capsicum-fed rats showed 51.7% (capsicum 1%) and 58.1% (capsicum 10%) decrease in caries score in part I experiment. 21.5% and 33.6% decrcase were recorded for part II experiment. 3. Garlic-fed rats showed 44.5% (garlic 0.5%), 23.0% (garlic 5%) and 53.7% (garlic 10%) decrease in caries score. 4. Sodium chloride-fed rats showed 122.6% (NaCl 3%) increase in caries score. 5. Rats which were fed diet containing 2% Na_2HPO_4 showed 26.7% decrease in caries score and further 41.6% decrease when 10ppm fluoride was given simultaneously. 6. 28.4% and 31.5% decrease in caries score were recorded for rats which drank water containing 50ppm of Mg and Zn.

신경회로망을 이용한 시변 동적 시스템의 적응제어

이상관,박영식,최부귀 東亞大學校 附設 情報通信硏究所 1995 情報通信硏究所論文誌 Vol.3 No.1

본 논문에서는 시변 동적 시스템의 적응제어를 위한 신경회로망의 응용에 대해 제안한다.플랜트를 제어하기 위하여 역동적 신경회로망 모델을 사용하는 학습이 필요하다. 제안된 시스템은 제어회로망과 학습회로망으로 구성된다. 제어회로망은 제어된 플랜트와 직렬로 구성한다. 학습회로망은 회로망이 동작하는 동안 역 동적 학습을 하기 위해 플랜트와 병렬로 구성한다. 제어회로망은 궤환오차와 원하는 플랜트의 출력을 입력으로 하여 제어 신호을 발생한다. 시뮬레이션 결과를 통해서 본 연구에 제안된 적응 추적제어 문제의 응용 가능성을 확인한다. In this paper an application of neural networks to the adaptive control of time-varying dynamic systems is proposed. The essence of the approach adopted was to use a neural network model of the inverse dynamics of a plant to control that plant. The proposed control system comprises a control network and a training network. The control network is placed in series with the controlled plant. The training network is positioned across the plant to learn its inverse dynamics during operation. The control network generates control signals to the plant, taking as its inputs the desired plant output and the feedback error. Simulation results are presented to illustrate the applicability of the proposed scheme to adaptive tracking control problems.