영산강 상류일부의 水質汚染에 관한 調査硏究 : 산동교를 중심해서

류일광,이치영,강영식,김관천 광주보건대학 1990 論文集 Vol.15 No.-

Water quality of Young San river was examined for 3 months from April 12, 1989. to Jun 12, 1989. For checking the water quality 4 sampling position were selected. The results we obtained are as follows. 1. The average for Young San river was 6.6∼7.4 of pH, 3.6∼8.7mg/ℓof Dissolved dxygen (DO), 18.67∼33.47mg/ℓof chemical oxygen demand (COD), 47.66∼250mg/ℓ of suspended solid (SS), 37.83∼114.66mg/ℓ of N-hexane abstraction maters, 5.16∼6.63mg/ℓ of BOD. 2. The monthly average for Young San river was 6.5∼7.5 pH,3.5∼10.4mg/ℓ of DO. 15.07∼48.90mg/ℓ COD, 6.2∼10.2 mg/ℓ of BOC, 2∼250mg/ℓ of SS. 2∼124mg/ℓ of N-hexane abstraction mater, in April, 6.2∼7.5 of pH, 3.5∼8.2mg/ℓ of DO, 2.4∼5.7mg/ℓ BOD, 18.21∼32mg/ℓ of COD, 16∼123mg/ℓ of SS, 9.5∼95mg/ℓ of N-hexane abstraction matter, in May 6.9∼7.5 of pH, 3.8∼7.50mg/ℓ of pH, 3.8∼7.50mg/ℓ of DO, 4.0∼5.8mg/ℓ of BOD, 18.23∼of COD, 123∼163mg/ℓ of SS, 102∼125mg/ℓ of N-hexane abstraction matter in Jun. 3. The sampling positions averag for Young San San river was 6.9∼7.5 of pH, 7.5∼10.4mg/ℓ of DO, 15.07∼22.8mg/ℓ of COD, 3.5∼6.2mg/ℓ of BOD, 2∼125mg/ℓ of ss, 2∼102mg/ℓ of N-hexane abstraction matter in posiction 1.7.3∼7.5 of pH, 6.0∼7.5mg/ℓ of DO, 46.25∼52.05mg/ℓ of COD, 2.4∼8.5mg/ℓ of BOD, 58∼123mg/ℓ of SS, 62∼120mg/ℓ of N-hexane abstration matter in position 2. 6.2∼7.3 of pH, of pH, 4.23∼6.7mg/ℓ of DO, 4.9∼7,9mg/ℓ of BOD, 18.23∼36.75mg/ℓ of COD, 89∼152mg/ℓ of SS, 75∼123mg/ℓ of N-hexane abstraction matter, in position 3, 6.5∼7.2 of pH, 4.0∼10.2mg/ℓ of BOD, 3.5∼3.82mg/ℓ of DO, 23.6∼52.32mg/ℓ of COD, 123∼250mg/ℓ of SS, 95∼125mg/ℓ of N-hexane abstraction matter in position 4.

개의 상후두신경 뇌간유발반응에 대한 실험적 연구

김광문,김영호,김광문,김영호 대한이비인후과학회 1998 대한이비인후과학회지 두경부외과학 Vol.41 No.4

극단 저체중 신생아에서의 동맥관 개존증 결찰술을 위한 진정맥 마취 경험 2례

최영균,고명진,이상은,조광래,김영환,임세훈,이정한,이근무,정순호,김영재,신치만 仁濟大學校 白病院 2010 仁濟醫學 Vol.31 No.-

Running title: Cases of anesthesia for extremely low birth weight infant. Extremely low birth weight infants (birth weight < 1000 g) are prone to various morbidities such as respiratory distress syndrome, intraventricular hemorrhage, periventricular leukomalacia, patent ductus arteriosus, necrotizing enterocolitis and retinopathy. To accomplish successful anesthetic management, many precautions must be continuously taken during the operation. First, inspired oxygen concentration should be adjusted to avoid oxygen toxicity. Second, body temperature must be maintained adequately. Third, hemodynamic parameters should be kept stable. We report 2 cases of successful anesthetic management for extremely low birth weight infant who underwent ligation of patent ductus arteriosus at the neonatal intensive care unit.

제1형 당뇨병 환자에서 췌도세포 동종이식의 반복시행

양태영,정인경,서인아,오은영,조건영,오승훈,김성주,정재훈,민용기,이명식,이문규,김광원,도영수,주성욱 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.4

연구배경:췌도세포 이식은 시술이 간편하고 안전하기 때문에 반복이식이 가능하여 그 동안 여러 센터에서 반복이식의 성공을 보고한 바 있다. 국내에서도 본 병원을 비롯하여 몇몇 센터에서 췌도세포 이식이 활발히 시도되고 있으나 사람에서의 동종이식 및 동종 이식의 반복시행은 보고된 바 없는 실정이다. 저자 등은 국내 최초로 췌도세포 동종이식을 시행하여, 혈청 C­펩타이드가 증가하고 인슐린 요구량이 감소하였으며, 혈당농도와 당화혈색소의 안정을 보였다. 그러나 이식 70일 후 다시 인슐린 요구량이 증가하고 C­펩타이드가 감소하여 췌도세포 이식을 다시 시행한 바 있다. 2차 이식 후 70여일이 지난 현재 다시 혈청 C­펩타이드가 증가하고, 인슐린 요구량이 더 감소되어 췌도세포 이식에서 반복 시행의 유용성을 보고하는 바이다. 방법:환자는 32세 남자로 17년전 당뇨병 진단 후 인슐린 치료 중이었으며, 3년전 부터는 만성신부전증으로 혈액툭석을 하고있었다. 1999년 12월 25일 신장이식을 하였고 3일 후 췌도이식을 하였으며, 두번째 이식은 70일 후 시행하였다. 췌도분리는 변형된 Recordi방법과 비연속성 자당 농도차(discontinuous density gradient)를 이용하였으며 분리한 췌도는 배양 후 환자의 신장기능이 정상화되고 미생물학 검사에서 음성을 확인한 후 경피경간으로 간문맥을 접근하여 16G 폴리 에틸렌 카테터를 이용하여 간실질에 이식하였다. 결과:1차 췌도이식시 순수분리전 췌도수는 210,000개, 순수분리후 획득한 췌도수는 90,000개, 순도 95%, 세포양 1.0mL 이었고, 2차 췌도이식시 순수분리전 췌도수는 420,000개, 순수분리후 획득한 췌도수는 370,000개, 순도 95%, 세포양 1.5mL 이었다. 1,2차 모두 간문맥을 통해 약 20분에 걸쳐 간실질에 주입하였다. 췌도이식전 인슐린요구량은 75∼75U/일, HbA1e 8∼10%, C­펩타이드 0.6ng/mL 였으며, 1차 췌도이식수 7일째 인슐린 요구량은 40U/일, C­펩타이드 1.5ng/mL, FPS 109mg/mL 였고, 40일 추적관찰 후 인슐린 요구량이 36U/일, C­펩타이드 1.8ng/mL, HbA1e 6.5∼7.0%로 안정되었다. 그러나 이식 50일째부터 인슐린 요구량이 50∼56U/일, C­펩타이드 0.6ng/mL, FPS 130∼200mg/dL로 혈당 조절이 불안정하여 다시 췌도이식을 시행하였다. 2차 이식후 50일이 경과한 현재, 인슐린 요구량은 26U/일, C­펩타이드 1.8ng/mL, FPS 90∼120mg/dL로 다시 안정되었다. 결론:췌도이식은 반복이식이 가능하며, 본 환자의 경우 인슐린요구량 감소, 혈당의 안정화 및 C­펩타이드가 상승하여 이식한 췌도의 기능을 확인할 수 있었고, 향후 스테로이드 등 면역억제가 유지 용량으로 감량되면 인슐린 요구량은 더 감소될 것으로 기대된다. Over the past 20 years, significant advances have been made in human islet transplantation. However, cases of prolonged insulin independence after islet allotransplantation have rarely been reported and over time, a slight, gradual decrease in insulin secretion appears to occur, as suggested by the lower C-peptide. Although preliminary clinical success achieved over the past few years has been considerably higher with whole pancreatic transplant than with isolated islet grafts, both approaches remain experimental. Islet grafts might gain, over time, increasing credibility and might eventually provide an easier alternative in terms of grafting procedures and patient management, as compared with the more "traumatizing" whole-pancreas transplantation. Also, using islet, re-transplantation is possible. But it is not known whether re-transplantation of islet could be suitable for those patients who lost grafted islet function. The aim of the present study was to investigate the benefits of re-transplantation of islet in previously simultaneous islets-kidney transplant (SIK) patient who have lost graft function. Methods : The recipient was a 32 year old male. First islet transplantation was underwent at December 25, 1999. However, the grafted islets lost function after 70 days. So we performed re-transplantation of islets. The isolation of islet was conducted sterilely on a laminarflow hood and isolated by a modified Recordimethod. The islet was injected slowly into the liver via a cannular placed in the potalvein for 20 minutes. Results : Transplanted islets were 90,000 IEq at first islet transplantation, 370,000 IEq at second islet transplantation. The insulin requirement was reduced from 75-85 to 35-40 U/day, the basal C-peptide level was 1.5 ng/mL at 7 days posttransplant Unfortunately, the grafted islets lost function after 70 days. After second transplantation, the insulin requirement was reduced to 26 U/day. Conclusions : Despite the continuous need for exogenous insulin therapy, islet transplantation can prevent wide glucose fluctuations, thus resulting in normalization of glycemic control and improvement in HbAlc, and also, show that islets can be successfully and safely re-transplanted intraportally in patients who have lost previously grafted islet function (J Kor Diabetes Asso 457~466, 2000).

Population Dynamics of Zacco platypus in Gap-Stream and Its Relation with Water Quality

Shin, Young-Eun,Choi, Ji-Woon,An, Kwang-Guk The Korean Society of Limnology 2009 생태와 환경 Vol.38 No.1

This study was to provide basic data for aquatic ecosystem research using fishes. Field sampling was carried out at five selected sites of Gap Stream, and fish samples, especially for a selection of sentinel species were collected three times in June, September, and October 2007. We analyzed total length distribution of Zacco platypus in relation with the season and the sampling sites, and then compared with total body weight, condition factor (K), and age distribution of the fish. The fish population data were compared with physico-chemical water quality, obtained from the Ministry of Environment, Korea. Water quality analysis showed a significant nutrient enrichment, based on total nitrogen (TN) and total phosphorus (TP), and organic matter pollution, based on biological oxygen demand (BOD) and chemical oxygen demand (COD) in the Site 5, which is directly influenced by wastewater disposal plant (WDP). Population analysis of the sentinel species showed that the total number of individuals, age distribution, and the population size-structure were influenced by the effluents from the WDP, and that reproductive failure of young-age population were evident in Site 5. According to the relation analysis of total weight to K, the disturbed population was mainly attributed to combined effects of habitat modifications and chemical degradations. Regression analysis of K values against water quality parameters showed significant (p<0.05) positive relations with nutrient and organic matter contents. Our data suggest that the population structure using a sentinel fish species reflected the ambient water quality in the stream and that diagnosis of aquatic ecosystem health using Z. platypus population may be practical for water resource and ecosystem conservations.

김치의 제조 및 유통중 가스발생 방지에 관한 연구

윤경영,강미정,이광희,윤광섭,김광수 대구효성가톨릭대학교 식품과학연구소 1997 식품과학지 Vol.9 No.-

대량생산을 위한 김치의 산업화에 있어서 가장 큰 문제점인 유통시의 산패현장과 포장팽대 및 파손을 지연시켜 김치의 저장기간을 연장하고자 이산화탄소 흡수능력을 가진 물질을 가스흡수제로 처리하여 저장중 김치의 품질을 검토하였다. 품질평가를 위한 측정항목은 pH, 산도, 색상, 이산화탄소 함량 및 젖산균수 그리고 총균수를 측정하였다. 수산화칼륨, 수산화칼슘, 수산화나트륨과 같은 알칼리물질과 연화마그네슘, 염화칼슘과 같은 염화합물을 가스흡수제로 사용한 결과, 수산화칼륨과 수산화나트륨처리구가 타처리구에 비해 pH의 감소가 적었으며, 또한 산도의 증가가 다소 낮았다. 수산화칼륨과 수산화나트륨을 각각 0.25%, 0.5%, 0.75% 세단계의 농도로 처리한 결과 pH와 산도에 있어서 수산화칼륨처리구가 수산화나트륨처리구에 비해 높은 pH와 낮은 산도를 나타내었다. 또한 각 처리구의 농도에 따라 큰 차이가 나타나지 않아 간편성과 경제적인 면, 그리고 시각적인 면을 고려해 볼 때 0.25% 수산화칼륨처리구가 탄산가스흡수에 의해 김치의 저장기간을 연장하는데 다소 효과를 보였다. PE bag으로 포장된 수산화칼슘과 PE flim(0.02㎜)으로 포장한 0.25% 수산화칼륨을 가스흡수제로 사용하여 무처리구와 비교하여 저장중 김치의 품질을 검토하였다. 저장중 0.25% 수산화칼륨처리구와 시판용 김치의 가스흡수제인 수산화칼슘처리구를 비교할 때, 수산화칼륨처리구가 무처리구에 비해 pH 및 산도의 차이가 컸으며, 김치의 조직과 국물속에 용해되어 있는 이산화탄소의 함량도 적었다. 그리고 총균수에 있어서 타처리구에 비해 수산화칼륨처리구가 높은 값을 나타내었다. When kimchi was manufactured and circulated, largest question is rancidity phenomenon and packing swelling. This study was conducted to prevent these phenomenon and prolong the shelf-life of kimchi. KOH, NaOH, Ca(OH)_2, MgCl_(2), and CaCl_(2) were used to gas-absorbent. The pH of kimchi used KOH, NaOH was higher than the others. The acidity of kimchi used KOH, NaOH was lower than the others. The concentration of KOH, NaOH was 0.25%, 0.50%, 0.75%. The decrease of pH and increase of acidity that kimchi dealt with KOH were lower than kimchi used NaOH. There in no significant difference in concentration of gas-absorbent. Comparision of kimchi used 0.25% KOH and Ca(OH)_2. Ca(OH)_2 packaged PE bag and 0.25% KOH packaged PE film(0.02㎜) were used to gas-absorbent. On comparision to control, kimchi dealt with 0.25% KOH was higher than kimchi used Ca(OH)_2 at pH and acidity. The CO_(2) contents of kimchi dealt with 0.25% KOH was lowest. The number of total microbe kimchi dealt with 0.25% KOH was highest.

Tetracaine이 개의 大腦皮質 Na^+-K^+-ATPase의 活性에 미치는 影響

이진영,김광용,서영,서경종,박영순 圓光大學校 基礎自然科學硏究所 1990 基礎科學硏究誌 Vol.9 No.1

본 實驗은 tetracaine이 개의 大腦皮質의 Na^+ - K^+ - ATPase의 活性에 어떻게 影響을 미치는 가를 알아보기 위하여 실시한 것이다. 그 酵素에 대한 tetracatine의 沮害效果와 그 機作은 酵素反應速度論的 分析方法 즉 Dixon-plot, Lineweaver-Burk plot, Arrhenius-plot 등으로 分析하였다. 그 結果는 다음과 같다. 1. Na^+ - K^+ - ATPase는 全 ATPase와 Mg^++ - ATPase의 活性보다 tetracaine에 의하여 더 많은 沮害를 받는다. 2. Tetracaine의 50% 沮害效果는 沮害常數 5㎎/㎖와 동일하였다. 3. Tetracaine은 그 酵素의 Km값을 변화시키지 못하였으나 最高速度(Vmax)는 변화시키기 때문에 tetracaine은 可逆的 非競爭的 沮害劑로 作用한 것 같다. 4. Q_10 값은 tetracaine의 농도가 증가함에 따라 감소되는데 이 값으로 Arrhenius-plot를 作成해 본 結果 切點(transition state)이 나타나지 않으므로 tetracaine은 脂質에는 작용하지 않고 오직 Na^+ - K^+ - ATPase에 작용하는 것 같다. The present experiments were disigned to investigate the effect of tetracaine on the dog brain cortex Na^+ - K^+ - ATPase activity. The inhibitory effects of tetracaine on the enzyme avtivity and its mechanism were analyzed by Dixon plot, Lineweaver-Burk plot, and Arrhenius plot. The results obtained here were as follows : 1. The Na^+ - K^+ - ATPase activity was inhibited more than the total ATPase and Mg^++ - ATPase in the presence of tetracaine. 2. The concentration of 50% inhibition for tetracaine was identical with the inhibition constant (Ki), the Ki value was 5 ㎎/㎖ for tetracaine. 3. In this study tetracaine did not change the Km value (2.5) but decreased the Vmax of the Na^+ - K^+ - ATPase activity, suggesting that tetracaine acts as reversible and noncompetitive inhibitor on the enzyme. 4. Q_10 value and energies of activation (20.1 Kcal/mole) decreased as the concentrations of trtracaine increased and the transition state did not appeared. These results suggest that trtracaine inhibits the enzyme activity by reversible and noncompetitive inhibition.

한국인에서 혈소판 당단백 Ⅲa 유전자 다형성과 급성 관동맥 증후군의 관계

김광일,채인호,김효수,손대원,오병희,이명묵,박영배,최윤식,이영우 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.5

비강내 투여된 Midazolam의 용량에 따른 진정효과의 비교연구

김영광,김용기 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.1

The purpose of this study was to assess the sedative effect and physiological parameter of two doses of intranasal midazolam in sedating young children for dental treatment. Twenty-five uncooperative children, mean age of 36.6 months, who needed at least two separate restorative visits, requiring local anesthesia participated in this study. The patients randomly received either 0.2㎎/㎏ or 0.3㎎/㎏ of midazolam intranasally, with the alternate regimen administered at the second appointment. All the children were restrained in a Papoose board and were monitored with Pulse oximeter for their heart rate and oxygen saturation. Degree of alertness, crying, and movement were evaluated at the start of treatment and the subsequent seven intervals throughout the procedure. Evaluation of overall behavior at each session was performed by one investigator, blind to the dose, using a separate rating scale. The reliability of ratings was assessed by two investigators using videotapes of the procedures. Statistical analysis showed no significantly different behavior (P>.05) between children receiving the two different doses. Successful sedation, as assessed by lack of or minimal crying and/or movement that interrupted treatment, was observed in most of the treatment visits with both doses (mean score 4.48±1.58 for 0.2㎎/㎏ and 4.40±1.38 for 0.3㎎/㎏). No adverse effect was observed, and most of treatments were successfully completed without difficulties.

CFRP적층쉘의 적층구성 및 곡률반경에 따른 관통특성

임광희,조영재,이길성,심재기,김영남,양인영 朝鮮大學校 機械技術硏究所 2004 機械技術硏究 Vol.7 No.1

최근 탄소섬유 강화 복합재료(이하 CFRP)는 비강도, 비강성이 높아 경량화가 요구되는 우주항공분야에서 구조용 재료로 널리 이용되고 있다. 본 연구에서는 CFRP 적층쉘의 적층구성 및 곡률반경에 따른 관통 특성을 고찰 하고자 한다. 실험에 사용된 복합재료는 일방향 프리프레그시트로서 적층방법은 〔0₃/90₃〕, 〔0₂/90₂〕s,와 〔0₂/90₃/0〕s, 〔0/90₂/0〕 2계면과 4계면으로 적층하였다. 그리고 다양한 곡률 반경을 적용하였다. (R= 100, 150, ∞). 적층순서에 맞게 적층된 시험편은 경화온도 130℃에서 진공백 성형과정을 오토클레이브로 제작 하였으며 100x140mm 크기로 절단 하였다. 시험편은 강구로 관통시켰으며 ballistic-screen sensor로 속도를 측정하여 충격전 운동에너지와 충격 후 운동에너지를 구할 수 있다. 흡수에너지는 곡률이 커질수록 증가하였으며 4계면 시험편보다 〔0₃/90₃〕s 와 〔0₂/90₂〕s 적층구조를 갖는 2계면 시험편이 흡수에너지가 더 높게 나왔다. Currently, carbon-fiber reinforced plastics (CFRP) are widely used in both space and civil aircraft due to their superior stiffness and strength to weight ratios compared to conventional metallic materials. This paper is to study the effects of curvature and staking sequence on the penetration characteristics of CFRP laminated shell. Composite laminates used in this test are CFRP orthtropic laminated plates, which are stacked with two-interfaces 〔0₃/90₃〕s, 〔0₂/90₂〕s and 〔0₂/90₃/0〕s. 〔0/90₂/0〕s. They are manufactured to varied curvature radius (R= 100. 150, 200 mm and ∞). They are cured by heating to the appropriate hardening temperature(130℃) by mean of a heater at the vacuum bag of the autoclave. Test specimens were prepared with dimensions 100mmx140mm. When the specimen is subjected to transverse impact by a steel ball, the velocity of the steel ball was measured both before and after impact by determining the time for it to pass two ballistic-screen sensor located a known distance apart. Absorbed energy increased as the curvature increased. 〔0₃/90₃〕s and [0₂/90₂〕s specimens, which is small interlaminar number, was higher than fore interlaminar specimen.