http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Hyun-Soo Kim,Tae-UkHan,Shien-Young,Kwang-SoonShin,Chul-JoongKim,Jong-TaikKim,Hyun-SooKim 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.3
During the period from January to December of2001, a total of 3,391 swine sera were submited to urlaboratory from 256 farms for the diagnosis of porcinereproductive and respiratory syndrome (PRS). Theantibody to porcine reproductive and respiratorysyndrome virus (PRRSV) was tested by the indirectimunofluorescent antibody (IFA) test. Of the 256farms tested, 230 farms (89.8%) were positive for thePRRSV antibody was 52.1% (1765/3391). Most of thepigs eemed to be infected with PRSV at around 50to 60 days old. The seroprevalence of the antibodybecame higher with age, and peaked at around 100days old. More than one-third of the adult pigs,including boars, gilts, and sows, was positive for thePRSV antibody. The infection of PRSV was chronicand confined to growers and/or finishers in mostfarms. However, the antibody was detected in alproduction phases at some farms .
Expression of Angiostatin Using DNA-Based Semliki Forest Virus Replicon
Chul-Joong Kim,Jong-SooLee,Young-KiChoi,Kwang-SoonShin,Hyun-SooKim 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.1
Angiogenesis recognized as a critical factor inthe growth of tumor cels and plays a key role in thetumor metastasis. Recent studies for antiangiogenicsubstances are geting popular. The angiostatin, oneof the antiangiogenic substances, leads to theincreased apoptosis of the tumor cels by inhibitingthe neovascularization of the tumor. The angiostatinwas identified as the internal fragments of theplasminogen which has no antiangiogenic activity. Byproduced. In this tudy, we constructed the SFV-derivedDNA vector by employing the cytomegalovirusimediate arly enhancer/ promoter (CMV). Thisvector makes it possible to transfect the cells withDNA without the in vitro transcription proces. TheC-myc epitope and polyhistidine residue sequenceswere placed in downstream of the angiostatin gene tomake it eligible to detect the xpresed protein. Themurine Ig κ-chain V-J2-C signal sequence was placedin upstream to secrete the xpresed protein from thecels. We confirmed the xpresion of angiostatin
Hyun-Soo Kim,In-WookHwang,Su-MiKim,Chul-JoongKim,Kwang-SoonShin,Hyun-SooKim 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.1
The ORF5 gene ncodes a major envelopeglycoprotein (GP5), which is one of the thre majorproteins of porcine reproductive and respiratorysyndrome virus (PRRSV). The GP5 protein has benknown to be a 24.5-26kDa N-glycosylated envelopeprotein. The GP5 is involved in inducing neutralizingantibodies. For this reason, the GP5 is primary can-didate for the PRRSV subunit vaccine. To produce thecloned t h e O R F 5 g e n e f r o m P R S V C N V - 1 i n t o t h eSemliki Forest virus (SFV)-based expresion vector,resulting in recombinant pSFV-ORF5. By the infectionwith recombinant pSFV-ORF5 to BHK-21 cells, theGP5 expresion was confirmed by imunocytochemistryand imunobloting asay. The recombinant virusparticle harboring ORF5 gene was infectious to BHK-21and MARC-145. The RNA synthesis and expression ofGP5 in the infected cell was also confirmed byRT-PCR.