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崔光律 건국대학교 1987 論文集 Vol.24 No.1
In dieser Abhandlung, habe ich versucht die neue Auslegung u¨ber die aufschiebende wirkung von Anfechtungsklage im Verwaltungsprozess in Einklang mit neue Verwaltungsgerichtsordnung, die am I. oktober 1985 in kraft getreten hatte, vorzuzeigen, indem ich inla¨ndische, ausla¨ndische Lehren und Entscheidungen samt durchsehen habe. Inhaltsverzeichnis dieser Abhandlung wirt infolgenden drei Teile geteilt. (a) Einleitung (b) Bedeutung der aufschiebende Wirkung von Anfechtungsklage (c) Gegenstand der aufschiebende Wirkung von Anfechtungsklage (d) Voraussetzung fu¨r die aufschiebende Wirkung (e) Verfahren fu¨r die aufschiebende Wirkung (f) Schluss
Kwang Ryool Heo,Kwang Youll Lee,Sang Hyun Lee,Soon Je Jung,이선우,Byung Ju Moon 한국식물병리학회 2008 Plant Pathology Journal Vol.24 No.1
Seedling damping-off and bottom rot caused by Rhizoctonia solani are yield limiting diseases of crisphead lettuce. To provide biocontrol measure in the management of the diseases, biocontrol strain Pseudomonas aeruginosa LY-11 was isolated from lettuce rhizosphere and introduced into crisphead lettuce rhizosphere by the seed coating delivery method. Alginate was used as a coating material to generate beads containing 106-106.5 colony-forming units (CFUs) of viable bacterial cells of LY-11. When seeds germinated from the alginate beads containing the strain LY-11, the bacteria established mostly in plant rhizosphere to maintain at least 104 CFU per gram of plant tissues. Crisphead lettuce seedlings germinated from the entrapped seeds were less affected from damping-off and bottom rot with disease control values of 70.4% and 85.4% respectively. Although P. aeruginosa LY-11 colonized plant rhizosphere and not phyllosphere, the result indicated that bottom rot caused by the foliar inoculation of R. solani was effectively reduced by the rhizobacteria. All data suggested that immobilized rhizobacterial application in seeds by alginate coating could control damping-off and induce induced systemic resistance of crisphead lettuce to reduce bottom rot.
Lee, Kwang-Youll,Heo, Kwang-Ryool,Choi, Ki-Hyuck,Kong, Hyun-Gi,Nam, Jae-Sung,Yi, Young-Byung,Park, Seung-Hwan,Lee, Seon-Woo,Moon, Byung-Ju The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.4
A biocontrol bacterium Bacillus licheniformis N1 grown in nutrient broth showed no chitinolytic activity, while its genome contains a gene which encodes a chitinase. The gene for chitinase from B. licheniformis N1 was amplified by PCR and the deduced amino acid sequence analysis revealed that the chitinase exhibited over 95% identity with chitinases from other B. licheniformis strains. Escherichia coli cells carrying the recombinant plasmid displayed chitinase activity as revealed by the formation of a clear zone on chitin containing media, indicating that the gene could be expressed in E. coli cells. Chitinase gene expression in B. licheniformis N1 was not detected by RT-PCR analysis. The protein was over-expressed in E. coli BL21 (DE3) as a glutathione S-transferase fusion protein. The protein could also be produced in B. subtilis 168 strain carrying the chitinase gene of N1 strain. The crude protein extract from E. coli BL21 carrying GST fusion protein or culture supernatant of B. subtilis carrying the chitinase gene exhibited enzyme activity by hydrolyzing chitin analogs, 4-methylumbelliferyl-$\beta$-D-N,N'-diacetylchitobioside and 4-methylumbelliferyl-$\beta$-D-N,N',N"-triacetylchitotrioside. These results indicated that even though the chitinase gene is not expressed in the N1 strain, the coding region is functional and encodes an active chitinase enzyme. Furthermore, B. subtilis 168 transformants expressing the chitinase gene exhibited antifungal activity against Fulvia fulva by suppressing spore germination. Our results suggest that the proper engineering of the expression of the indigenous chitinase gene, which will lead to its expression in the biocontrol strain B. licheniformis N1, may further enhance its biocontrol activity.
Heo, Kwang-Ryool,Lee, Kwang-Youll,Lee, Sang-Hyun,Jung, Soon-Je,Lee, Seon-Woo,Moon, Byung-Ju The Korean Society of Plant Pathology 2008 Plant Pathology Journal Vol.24 No.1
Seedling damping-off and bottom rot caused by Rhizoctonia solani are yield limiting diseases of crisphead lettuce. To provide biocontrol measure in the management of the diseases, biocontrol strain Pseudomonas aeruginosa LY-11 was isolated from lettuce rhizosphere and introduced into crisphead lettuce rhizosphere by the seed coating delivery method. Alginate was used as a coating material to generate beads containing $10^6-10^{6.5}$ colony-forming units (CFUs) of viable bacterial cells of LY-11. When seeds germinated from the alginate beads containing the strain LY-11, the bacteria established mostly in plant rhizosphere to maintain at least $10^4$ CFU per gram of plant tissues. Crisphead lettuce seedlings germinated from the entrapped seeds were less affected from damping-off and bottom rot with disease control values of 70.4% and 85.4% respectively. Although P. aeruginosa LY-11 colonized plant rhizosphere and not phyllosphere, the result indicated that bottom rot caused by the foliar inoculation of R. solani was effectively reduced by the rhizobacteria. All data suggested that immobilized rhizobacterial application in seeds by alginate coating could control damping-off and induce induced systemic resistance of crisphead lettuce to reduce bottom rot.
골막하골형성 과정에 출현하는 조골세포의 활성도에 관한 조직 화학적 및 전자 현미경적 연구
김광회,박명률,이중달 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.2
Osteoblasts synthesize and secrete the osteoid, and participate in the calcium and phosphate regulation in the osteogenesis. Osteoblasts are derived from osteoprogenitor cells, which are mesenchymal stem cells committed to differentiate into the bone forming cells. There are, however, different opinions concerning development of osteoblasts from osteoprogenitor cells and bone forming processes involved by osteoblasts. Author has undertaken an experimental work to elucidate the mechanism by which the bone has been formed from subperiosteal osteoblasts. The experiment consisted by 32 Korean rabbits. Animals in each group received bilateral ostectomies of the femoral shaft of 5 mm in length, and controlateral bone shaft was transplanted. A chronologic observation was made in relation to the activity of alkaline phosphatase and electron microscopic feature of the subperiosteal osteoblasts. From gross, histologic, enzyme histochemical and electron microscopic observations, following results were obtained. 1. Osteoblasts derived from the peristeum adjacent to the ostectomy site began to proliferate on the day 4. Features of osteoprogenitor cells were indistinguishable from those of fibroblasts. 2. Alkaline phosphatase activity on the proliferating osteoblasts was markedly increased throughout the experimental period. Both osteoprogenitor cell and fibroblasts showed no activity on alkaline phophatase. 3. The osteoblasts became cubiodal in shape and had cytoplasmic processes. The cytoplasm were filled with extensive Golgi complex, rough endoplasmic reticulum, and secretory vacuoles. Poor cytoplasmic organelles were present in both fibroblasts and osteoprogenitor cells. 4. The osteoid began to lay down on the day 7 around osteoblasts. Osteoblasts embedded in osteoid, woven bone, and/or lamellar bone transformed into osteocytes. Alkaline phosphatase activity was weakly positive in osteocytes. Woven bone and lamellar bone because apparent on the day 21 and 30, respectively. In summary from the results obtained, osteoblasts play a most significant role in the osteogenesis during fracture repair. Alkaline phosphatase activity is remarkably prominent in the osteoblasts, the finding of which is well correlated to the amount of lysosomes observed in the electron microscopic study. Both histochemical and electron microscopic features of osteoprogenitor cells and fibroblasts are not distinctive between the two cell types.
자가면역 용혈성 빈혈과 심낭삼출이 동반된 하시모토 갑상선염 1예
이광우,손호영,강무일,유순집,강성구,박지원,여경아,차봉연,최주연,진종률 대한내분비학회 1998 Endocrinology and metabolism Vol.13 No.4
Hashimotos thyroiditis has been associated with a various autoimmune disorders. The immunologic mechanisms involved in the pathogenesis of these disorders have not always been thought to be the same. Although it was demonstrated that there were high prevalence of abnormal thyroid function and autoantibody in autoimmune hemolytic anemia(AIHA) and Fisher-Evans syndrome(FES), AIHA combined with Hashimotos thyroiditis is rare in Korean literature. It was suggested that a common immunologic mechanism may be involved in the pathogenesis of both disease and the possibility of multiple autoimmune syndrome might present in autoimmune hematologic disorders. We experienced a 74-year old woman with a 12-year history of a hypothyroidism due to Hashimotos thyroiditis was hospitalized with sudden development of warm AIHA with positive Direct & Indirect Coombs test and pericardial effusion. Her thyroid function test showed subclinical hypothyroidism with the maintenance dosage of levothyroxine(100pg/day). With glucocorticoid and plasmapheresis, AIHA and pericardial effusion were corrected successfully. It is suggested that the prudent immunologic study is needed for the anemia developed in patients with Hashimotos thyroiditis with or without hypothyroidism (J Kor Soc Endocrinol 13:622-628, 1998).