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Immuno-Affinity Chromatography에 의한 B. thuringiensis H9B균주의 모기살충성 내독소 단백질의 정제
김광현,배수장,이광배 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1
For purification of a 70kDa toxic protein of mosquitocidal delta-endotoxin from B. thuringiensis strain H9B, immuno-affinity chromatography was performed. After separation of 70kDa toxic proteins from the delta-endotoxin of the strain H9B on SDS-PAGE, the 70kDa toxic protein was subcutaneously injected into rabbit for making a polyclonal antibody. A anti-70kDa toxic protein was purified by a column chromatography packed with proteinA-sepharose 4B gels. The 70kDa toxic protein from delta-endotoxin of the strain H9B was also purified by an immuno-affinity chromatography packed with CNBr-activated sepharose 4B gels conjugated anti-70kDa toxic protein after elution with 1/10M citric acid-1/5M Na₂HPO₄buffer(pH3.2) containing 0.5M NaCl. The 70kDa toxic protein was purified through only one step-separation system, was demonstrated by SDS-PAGE and immunoblot.
무선 애드 혹 기반 액티브네트워크상에서 QoS지원에 관한 연구
李基源,裵振勝,丁燦赫,金玄郁,李光培,姜景仁,朴京培,文泰洙,郭承郁,河載承 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-
Abstract - In this thesis, we propose a QoS supporting method on wireless ad hoc-based active network for improving reliability. On the loss of route path, the existing DSR routing protocol has several problems with wireless network communication because of decease of data reception rate and increase of network load. The proposed method improves reliability of data transfer by using functions of active network. That is, by using functions of active nodes and active packets, the proposed method supports QoS with reservation transfer method that saves data packets on transfer at intermediate nodes with lost path and then re-transmit the packets after route reconfiguration.
MANET 라우팅 프로토콜 QoS 지원서비스의 성능평가에 관한 연구
鄭燦赫,金鉉郁,李光培,姜景仁,朴京培,劉忠烈,吳世德,裵振勝,趙榮泰 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-
In this paper, we evaluated two main QoS supporting routing protocol on wireless ad hoc network. At presence, wireless mobile communication focuses on how to efficiently support mobility of users more than QoS guarantee. However, in order to satisfy requirement of various applications which have been or will be served, QoS support service between source and destination becoming a very important issue. Of all routing protocols, DSR and AODV are very important routing protocol in MANET. So we simulated DSR and AODV QoS Routing Protocol Through simulation evaluation tool NS(Network Simulation) based on various environments.
배성범,이광형 崇實大學校 生産技術硏究所 2001 論文集 Vol.31 No.-
In recently, digital information, such as text, voice, image, is spreading rapidly in the world of the internet. It is easy to edit or store digital data through network. But, anyone could get a digital data as same as the original by copying. Illegal copy or distribution causes a serious problem of intellectual copyright infringement. To solve this problem, digital watermarking schemes have been studied by many researchers as copyright protection. This paper presents a watermarking algorithm in order to embed visually the recognizable pattern (such as Mark, Symbol, Stamping) into a image. We propose the effective watermarking method using the wavelet transform and PN code. The experimental results show that the proposed watermarking algorithm is superior to the other similar watermarking algorithm. We show that it is robust to common signal precessing and geometric transforms such as brightness, contrast, filtering, scaling and geometric deformations.
DiffServ 환경에서 Mobile Xcast 지원에 관한 연구
배진호,김태수,이광휘 국립7개대학공동논문집간행위원회 2003 공업기술연구 Vol.3 No.-
In the third generation of mobile computing environment, the applications that are compatible with small members of group in which the applications join the multicast sessions such as Internet phone, video conference and networked games are more prevalent. However, since the traditional multicast mechanisms supporting the mobile computing are suitable for large members of group in the multicast session, we need to consider a new mechanism to complement them. Therefore, in this paper we suggest a new multicast model that adopted explicit multicast(xcast), which has more gradual deployment with legacy Internet backbone. This proposed model is cost-effective and more scalable and guarantees the QoS in mobile computing.
자극제 사용 미분쇄 플라이애쉬 모르타르의 강도증진에 관한 실험적 연구
배수환,최광윤,정재동 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)
The purpose of this study is for the active use of the fly ash, which is a by-product of the combustion pulverizes coal thermal power plants, it should be used to compensate for the lack of landfill and for conservation of energy as the supplementary cementitious material, and to prove its possibility as the related products of the cement. First of all, the ordinary fly ash is ground in a special method and its fineness is controlled from 4000cm²/g to 6000 cm²/g, then replaced it with the 20% to 80% of the cement mortar to test of the physical properties. The first experiment is about the slow development of the strength of the fly ash mortar in early ages, and improves its strength with the activator Na2SO4, using a large amount of fly ash. On the other hand, in case of adding activator with steam curing, it rather shows the decrease of strength. This is that it needs to consider the influence by high temperature.
배지명,김광만,김경남 대한치과기재학회 1997 대한치과재료학회지 Vol.24 No.1
The colors of composite resins are changed with time due to intrinsic and extrinsic factors. This study was aimed to characterize the effect of each factor. Specimens(dia.15mm×thic.2mm) were prepared from Z-100(ZH), Aelitefil(AF), Photo Clearfil Bright(PF), Bisfil-2B(BF) and Clearfil FⅡ(CF). One group of samples was exposed to a xenon arc of 1,000W with a total dose of 410kJ/㎡ for 228hrs in a weathering chamber and then immersed in filtered coffee for 7 days. The other group of samples was immersed in coffee first and then exposed to the xenon arc. The color were measured before and after each treatment with a spectrophotometr according to the C.I.E. L*a*b* color system. Ra values of surface roughness, contact angles and SEM observations before and after accelerated aging were compared. Results were as follows : 1. In the accelerated aging and subsequent staining group, the color changes of AF and PF after aging and all materials after subsequent staining, were greater than the upper limit of acceptibility(ΔE=3.3). 2. In the staining and subsequent accelerated aging group, the color changes of BF after staining and AF and PF after subsequent aging, were greater than the upper limit of acceptibility. 3. AF and PF were sensitive to intrinsic factors, BF to extrinsic factors, and extrinsic factors were activated by accelerated aging in ZH and, CF. 4. The color changes by accelerated aging showed consistent results regardles of pre-staining while those by staining were affected by pre-aging. 5. Ra values of roughness after aging did not change significantly and had no relation to the color changes after aging and subsequent staining(P<0.05). 6. Though the contact angles of AF, BF and CF after aging were reduced significantly, they had no relation to the color changes after aging and subsequent staining(P<0.05). It was concluded that each of the discoloration features by accelerated aging and staining was detectable and could be clinically used by considering the characteristics of the materials which are sensitive to intrinsic and/or extrinsic discoloration factors.
수유방법 및 기간이 아동의 타액내 환원효소활성검사성적과 유치우식경험도에 끼친 영향
배상만,노동주,김대업,이광희 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.3
The purpose of this study was to investigate the effects of the lactation of human or bovine milk and the method of using nursing bottles on the salivary invertase test scores and deciduous caries experience of children. 328 preschool children (boys 178, girls 150) in Iksan city were selected for this study. The parents of the children were asked to answer the questionnaire about the lactation of human or bovine milk and the time of using nursing bottles. The data were obtained from the salvary invertase activity score,(Resazurin Disc Test) deciduous caries experience and the questionnaire. The result were as follows. The invertase activity and caries experience of the human milk higher than that of the bovine milk group(p<0.05). There were significant positive relationships between the lactation period and the invertase activity and between the lactation period and the caries experience(p<0.05). Caries experience had a tendency to increase as the time of using nursing bottles increase(p>0.05).