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Woo‑Ri Shin,Mun‑Jong Lee,SEKHONSIMRANJEET SINGH,Ji Hun Kim,Sun Chang Kim,Byung‑Kwan Cho,Ji‑Young Ahn,Yang‑Hoon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3
Background Development of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer herbicide tolerance and insect resistance. Detection system of GM crops is important for safety evaluation before its consumption. Objective The purpose of this research is to detect GM crops, especially PAT, in food-samples. Results The bar gene (PAT protein, herbicide resistant) was cloned in pGEX-4T-1 and expressed by E. coli. The high-affinity PAT-specific single-stranded DNA (ssDNA) aptamers were obtained from a random DNA library. MOE docking study was performed to identify the potential binding region of the selected aptamers on PAT. Aptamer-linked immobilized sorbent assay (ALISA) method was used to detect PAT. Conclusion We screened aptamer against PAT for developing an efficient detection method. The selected PAT specific aptamers, HRPA-05 and HRPA-07, showed the distinct target binding behaviors, and detected PAT protein by aptamer-linked immobilized sorbent assay method with high efficiency and selectivity.
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李相泰,蘇雄永,田寬培,李愚喆 全北大學校 生物學硏究所 1981 生物學硏究年報 Vol.2 No.-
The present paper is an introduction to the whole project of "The systematic studies on Korean Betulaceae". Three studies under the project were performed this year and presented in this report : a review of taxonomic literatures and the construction of distribution maps by Woo-Tchul Lee, pollen morphology by Sang-Tae Lee and Moo-Yeol Kim, and wood anatomy by Woong-Young Soh and Kwan-Bae Jeon.
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禹寬壕 홍익대학교 1997 弘大論叢 Vol.29 No.-
The Kakiemon is one of the many hard porcelain forms produced from the Arita in Kyushu, in Japan. this particular style has evidently led to represent the porcelain tradition of japan for 14 generations. moreover, it is renowned all over the world and in actual fact, it accounts for the existence of european porcelain today. The Elector of Saxony, Auguste the Strong, ardently appreciated Japanese porcelain in actual fact, This is clearly shown in the naming of Zwinger Palace, which was specifically constructed for collecting Japanese and Chinese porcelain. It came to be named as the Japanese Palace. Although the Japanese porcelain, during this period, was the Imariyaki, name gonerally given for Aritayaki porcelain, among all over them, the main form was believed to have been the Kakiemon. Auguste's fanatical passion for collecting Chinese and Japanese Porcelain, eventually led him to initiate his own production. In order to put this into practice, he assembled a group of alchemists and so on. Finally, he succeeded in making porcelain and in doing so, he established the grounds for the development of European Porcelain. Hence he attributed to its existence today. Yet, the patterns applied in early European Porcelain, were not original. Rather, they were mere copied forms of the Chinese and Japanese Porcelain. Of all the forms, the Kakiemon seem to have been the main influence, for this style had become familiar to the European aesthetics as well as their sentiments. The Kakiemon style did not dominate the decorative patterns found European Porcelain for along time, but the majority of the early porcelains, used patterns found in Kakiemon porcelain. The patterns were copied until the development of transfers. The change took place as the Kakiemon was gradually being interpreted in a European way. The use of blue on a white clay body is the distinct characteristic in the Kakiemon technique. Akae, a low temperature colouring agent, can also be applied for fine expressions. The technique of making white clay bodies was first introduced to the Japanese by the Chosen potter Lee Sampei, who was a Japanese Invasion of 1592(also referred to as Hideyoshi's Invasion), The use of the white clay body began with the discovery of kaolin in the Izumiyama of Arita. Also, the use of Akae originated from China but together with its technical introduction, it soon became interpreted in a Japanese way. With the settling of the style, the Kakiemon style was Further developed, as mentioned earlier, due to the European interest and admiration for eastern porcelain as well as their curiosity in the Chinese social condition of that particular period. At the time, the European demands in porcelain were fulfilled by a particular porcelain production place in Chingteichin, but social instability at the end of the Ming Dynasty and the beginning of the Qing Dynasty, brought about a halt in the porcelain trade. Therefore the European tradesmen took the Chinese product, as a sample and they commissioned their requests to the Japanese. Consequently, the potters of Arita urged the expansion of production equipments and the development of skills in order to manage vast amounts of trade. Hence attaining the ability to produce high quality porcelain. The Kakiemon was also one of the potteries trying to meet the demands of the period but the development of the Akae technique raised its reputation reality, to become an acclaimed brand. This study concentrates on the development and the transition of the Kakiemon forms. It aims to be used as the basis for researching on Japanese porcelain. In order to do this, the history of the Arita region has been taken into consideration. In a country, which professes in its developed ceramics culture, its porcelain, in particular, both aesthetically and technically is classified in a highly-rated field amongst all the other different types of ceramics. The Japanese efforts, in commercializing porcelain robots made for musical shows, does not come across as an entirely profitable example.
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우훈희,홍지숙,서정권,이관영,이정민 한국공업화학회 2002 응용화학 Vol.6 No.2
In order to seek optimum conditions for ZSM-5 formation in the absence of organic template,the effects of both starting composition and water glass as silica source were examined. Water glass was most inexpensive among silica sources, but this was difficulty in reactant control because of contained much alkali ingredient, Considering environmental problem and economic performance,chose alkali drawing-out method to use methanol newly to remove alkali ingredient of waterglass. Characterization of ZSM-5 was carried out by means of XRD, SEM.
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초·중·고 학생들의 과학 탐구 능력 추이 분석을 위한 종단적 연구
우종옥,김범기,허명,김찬종,양일호,최관순,김태선 한국교원대학교 과학교육연구소 1999 청람과학교육연구논총 Vol.9 No.1
본 연구에서는 우리 나라 초·중·고등학교 학생들의 과학 탐구 능력에 대한 종단적 추이를 분석하였다. 1997년에는 우종옥 등(1998)이 개발한 검사지를 사용하였으며, 1999년에는 이를 일부만 수정하여 사용하였다. 조사 대상은 성별, 지역별 등을 고려하여 표집하였다. 조사된 자료는 각각 횡단적으로 분석하고, 이를 바탕으로 학생들의 과학 탐구능력이 어떻게 변해 가는지 종단적 추이를 분석하였다. 조사 결과 초등학생들의 과학 탐구 능력은 유의미한차이가 없었으나, 중·고등학생들의 과학 탐구 능력은 유의미하게 향상된 것으로 나타났다. 학교 과학 교육의 질을 과학적으로 관리하기 위해서는 교육과정이 추구하는 교육 목표의 달성이 성공적으로 이루어지고 있는지를 국가수준에서 주기적으로 측정하여 부진의 원인을 진단하고, 개선 방안을 계속 연구하여야 할 것이다. The longitudinal trend of elementary and secondary students' science process skills were investigated to monitor the progress of science learning. Science process skill test, developedin an earlier study on developing a national science process skills assessment system, was revised and administered for the study on March, 1999. Subjects were sampled nation wide according to sex and region. The results of this assessments were compared to those administered on February, 1997. The results showed little difference on science process skills among elementary school students. However, secondary students showed marked increase in science process skills. More systematic and long-term research for assessing science process skills should be planned and performed periodically to provide empirical and foundational data for educational policy making.
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