인체구강상피세포의 배양조건에 대한 연구

박종수,최희웅,이수관,김수복,고광삼 中央醫學社 1990 中央醫學 Vol.55 No.2

Human epithelial cell cultures were initiated from explants of normal human oral mucosa. Growth parameter and cell type were assessed by DNA concentration and morphology of the cell lines. Tissues were excised and epithelial cells isolated by collagenase digestion. Spermine was used in order to inhibit overgrowth of the epithelial cells by fibroblast. The effects of different medium formulations were compared. Cultures maintained on the same volume of RPMI 1640 and Dulbecco's modified minimum essential medium containing 10 % fetal bovine serum, 10 ?g/ml of insulin, lng/ml of somatomedin and 10 ng/ml of epidermal growth factor showed optimal and maximal growth. For each cultured human oral epithelial cell the DNA content was in the- range of 5.7 pg to 6.6 pg. This culture system provides epithelial cells that can be used for a variety of dental and biochemical studies.

요골동맥과 복재정맥을 이용한 관상동맥우회술의 조기성적

김관창,원태희,한재진,최수승,안재호 이화여자대학교 의과학연구소 2008 EMJ (Ewha medical journal) Vol.31 No.2

Background:Increasing interest and use of arterial conduits is based on the better patency of left internal thoracic artery(LITA) than that of saphenous vein(SV) graft. We compared the early result of coronary artery bypass grafting(CABG) using LITA and radial artery(RA group) with CABG using LITA and SV only(SV group). Material and Method:We compared the early operative results of 6 cases in RA group with 18 cases in SV group selected from 24 cases that had CABG between January 2006 and December 2006. We analyzed each group on the preoperative risk factors and operative results. Results:We can’t find significant differences in clinical and hemodynamic characteristics before surgery. There were no statically significant difference between two groups in operative mortality and each morbidities(postoperative intraaortic balloon pump insertion, bleeding, stroke, perioperative myocardial infarction, wound dehiscence), respectively. However, the overall incidence of conventional CABG using cardiopulmonary bypass was higher in RA group compared to SV group(p=0.016). Accordingly, RA group had longer duration of ventilation time(p=0.004) and ICU stay(p=0.003) than SV group with statically significant difference between two groups in hospital stay. The graft patency on postoperative coronary angiography or computerized tomographic angiography at 7-14 days after operation in both group patients were 100%(includeing LITA, RA and SV). Conclusion:We had early good operative results in RA group and SV group. 관상동맥우회술에서 내흉동맥의 장기개통률이 복재정맥보다 우수하다는 것이 알려짐과 더불어 두 개의 내흉동맥을 이용한 군에서의 장기 임상 성적이 하나의 내흉동맥만을 이용한 군에서의 성적보다 우월하다 것이 알려지면서 최근에는 동맥이식편의 사용이 증가하고 있다1)2). 좌내흉동맥이 동맥이식편중 가장 많이 그리고 오래 전부터 사용되어왔고 그 장기 개통률이 매우 우수한 것으로 증명되면서, 이식편으로 사용 가능한 다른 동맥이식편에 대한 사용이 늘어나고 있는 추세이다. 좌내흉동맥 이외에 사용될 수 있는 동맥이식편으로는 요골동맥, 우내흉동맥, 우위망막동맥 등이 있다. 이 중 우내흉동맥은 좌내흉동맥과 같이 사용한 경우 장기 생존율이 높다는 보고도 있지만 가용길이가 짧아 이용에 한계가 있고 수술시간이 길어지며 상처 합병증과 기술적인 문제 등이 있다2). 요골동맥은 Carpentier 등3)에 의해 1970년대 초 처음 이식편으로 소개되었으나 추적 관상동맥조영술상 높은 이식편 부전으로 인해 사용이 중단되었다가 1989년 다시 소개되어 주목을 받게 되었고4), 요골동맥박리술의 기술적인 변형과 항연축제의 사용으로 요골동맥의 연축을 최소화하여 사용하면서 많은 저자들에 의해 좋은 임상 성적과 높은 개통률을 보이고 있다5-7). 본 이화여자대학교 목동병원 흉부외과에서도 요골동맥을 좌내흉동맥에 이은 두 번째 동맥이식편으로 사용하고 있는데 본 연구에서는 본원에서 경험한 요골동맥을 사용하여 시행한 관상동맥우회술의 임상 성적과 수술 후 관상동맥조영술 혹은 컴퓨터 단층 혈관조영술 결과를 분석해 보고 아울러 이를 요골동맥을 사용하지 않고 내흉동맥과 복재정맥만을 이용한 군의 성적과 비교 분석해 보았다.

의사소통 능력신장을 위한 Communication Practice의 활용

柳官洙 조선대학교 외국문화연구소 1987 外國文化硏究 Vol.10 No.1

데이타흐름 시스템에서의 성능평가에 관한 연구

유관종,최종석,신지수,변승환 충남대학교 자연과학연구소 1989 忠南科學硏究誌 Vol.16 No.1

A concept of data flow computer is a radical departure from that of Von Neumann type computers since the central concept of sequential execution is discarded. Data flow model offers an entirely new way of view the execution of program. This paper describes the data flow graph partitioning algorithm for enhancing the efficiency of the data flow architecture which consists of Processing Elements, Distributor, Arbitrator and Global Memory, and evaluate the performance of the proposed data flow system.

WH-question使用에 나타난 오류분석

류관수 조선대학교 인문학연구소 1983 外國文化硏究 Vol.6 No.1

TFT-LCD bus line용 ITO/AlNd의 접촉저항의 특성에 관한 연구

김동식,정관수 경희대학교 산학협력기술연구원 1999 산학협력기술연구논문집 Vol.5 No.-

최근 TFT-LCD용 panel이 대형화, 고세정화됨에 따라 저저항 배선 재료의 개발이 요구되고 있다 이에 따라 저저항 배선 재료에 대한 개발 및 ITO와의 접촉저항을 줄이기 위한 연구가 활발히 진행되고 있다. 본 논문에서는 DC magetron sputtering system을 이용하여 다양한 조건하에서 AlNd 박막을 성장하여 각각의 sample들은 SEM, AFM, XRD와 4-point-probe를 사용하여 그 특성을 조사하였고, AIND과 ITO간의 접촉저항을 측정하였다. 본 실험에서의 최적 조건은 120℃, 125W, 0.4Pa, 30 :SCCM(Ar), 350℃-20 min. annealing에서 얻을 수 있었고, 그때의 AlNd(2%wt.)의 저항값은 약 4 Ωcm 이다. AlNd과 ITO간의 가장 낮은 접촉 저항값의 조건은 300℃. Ar 30sccm. O_2 분위기였고 그 값은 약 110Ω㎠이었다

梅堯臣 田園詩의 內容特徵分析

文寬洙 世明大學校 1992 世明論叢 Vol.2 No.-

本論文的硏究範圍是梅堯臣的田園詩, 其內容可分爲五種:第一反映農民族苦的詩篇, 第二 描寫田園景色的詩篇, 第三 描寫農事的詩篇, 第四 敍述閒適的農村生活的詩篇, 第五 歸向田園的詩篇等, 在本論文中主要討論的內容是反映農民疾苦的作品與描寫田園生活的哀歡的作品. 梅堯臣田園詩中最重要的內容當係反映農民生活的什篇. 當時農村經濟窮因, 加以爲政者的失政, 使農民生活極爲困苦. 梅堯臣能구反映沚們的疾苦, 告訴貧官汚史的惡行. 這一點是宋朝田園詩的重要內容. 在形式上用平易的詩語自然而然的寫出田園景色和田園生活中的哀歡, 使讀者更生動庚感興.

西海岸地域 水産業發展을 위한 公開講座프로그램의 開發에 관한 硏究

金秀寬,金在璇,李禹昌,房基俊 군산대학교 1991 群山水産專門大學 硏究報告 Vol.25 No.1

The purpose of this study is to develop the extension program for the improvement of fisheries industry in the western coastal area by making the workers in fisheries understand its environment and obtain the knowledge and the skill about it. To attain the above purpose, theoretical and empirical approaches are used in this study. The extention program developed by this study will be a useful source material to the institutes which plan social education for the workers in fisheries. Suggestions for the activation and settlement of the social education are as follows. First, the frequency of the social education about fisheries will have to be increased. Second, the main institutes of social education will have to be diversed to specialize the education line and method. Third, the required subjects in pratical field will have to be selected and educated for improving the effect of social education.

Acanthopanax chiisanensis의 揷木方法과 發根에 미치는 諸要因에 관한 硏究(Ⅱ)

金寬洙 대전대학교 기초과학연구소 1990 自然科學 Vol.1 No.-

Acanthopanax chiisanensis植生調査와 揷木繁植方法의 硏究結果를 要約하면 다음과 같다. 錄枝部位에 따른 揷木의 이용과 發根關係에 있어서는 粗砂土區보다 마사토區에서 각 樹齡別 共히 높은 發根値를 보였다. 또 錄枝部位에 따른 揷穗의 利用과 發根關係에 있어서는 半熟枝의 基部를 利用한 揷穗의 發根率과 發根量이 현저히 높게 나타났고 先端部分을 利用한 것이 가장 低調한 경향이 있다. 揷穗의 葉數와 莖長에 따른 發根關係는 2葉 10㎝인 揷穗의 發根成績이 가장 높았고, 有葉인 揷穗에 비하면 無葉인 揷穗의 發根率과 發根量이 가장 低調한 경향이 있다. 그리고 床土의 土性과 揷木時期에 따른 1~2年生枝의 發根關係는 마사토區가 粗砂土區에 비하여 揷木時期와 각 揷木別 共히 發根率 및 發根量이 높은 경향을 나타냈으며 最高置는 6月 16日 마사토에 揷木한 1年生枝 2葉 莖長이 10㎝인 揷穗였고 가장 저조한 것은 7月 16日 粗砂土에 揷木한 2年生枝 2葉 莖長이 15㎝인 揷穗였다. The results of vegetative investigation and cut breeding method of Acanthopanax chiisanensis are as follows. In the rooting relationship on every age of tree with a seedpolt soil. The unriped soil came out higher than that in the crude sand soil. In harness of scion and rooting relationship on the shoots region, the rooting percentage and amount of scion used the semi-growth branch had the most and those of the foremosttip region had the least. In the rooting relationship on number of leaf and stem length, 2 leaf scion as length as 10㎝ had the highest in rooting percentage and amount. And the rooting percentage and amount of scion with leaf came out higher than those of scion without leaf. In the rooting relationship of 1 or 2 year branches on the texture of seed plot soil and cutting time, the rooting percentage and amount in the unriped soil plot came out higher than those in the crude sand soil plot. 2 leaf scions as length as 10㎝, 1 year branch which was planted in the unriped soil the 16th, June had the highest and 2 leaf scion as length as 15㎝, 2 year branches had the least, Which was planted in the crude sand soil on the 16th, August.

오갈피나무의 揷木方法과 發根에 미치는 諸要因에 관한 硏究

金寬洙 大田大學校 1984 論文集 Vol.3 No.1

The result of vegetetive investigation and cut breeding method of Acanthopanax sessiliflorus are as follows. 1. In the rooting relationship on every age of tree with a seedplot soil, The unriped soil came out higher than that in the crude sand soil. 2. In harness of scion and rooting relationship on the shoots region, the rooting percentage and amount of scion used the semi-growth branch had the most and those of the foremost tip region had the least. 3. In the rooting relationship on number of leaf and stem length, 2 leaf scion as length as 10cm had the highest in rooting percentage and amount. And the rooting percentage and amount of scion with leaf came out higher than those of scion without leaf. 4. In the rooting relationship of 1 or 2 year branches on the texture of seed plot soil and cutting time, the rooting percentage and amount in the unriped soil plot came out higher than those in the crude sand soil plot.2 leaf scions as length as 10 cm, 1 year branch which was planted in the unriped soil the 16 th, June had the highest and 2 leaf scion as length as 15 cm, 2 year branches had the least, Which was planted in the crude sand soil on the 16 th, August.