인간 재조합 인터루긴-32 면역조절작용에 대한 유세포 분석

이광수,김영관,채정일,심정현,김은미,강형식,김수현,윤도영,명평근 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

Xenotransplantation of porcine organs has the potential to overcome the severe shortage of human tissues and organ available for human transplantation. however, it remains various hurdles for clinical xenotransplantation. In pig and mouse xenotransplantation, porcine xenograft evoke a strong cellular rejection response in immunocompetent host and grafts are destroyed within a week. This cellular immune response could involved both T cells and NK cells. A number of groups have shown that human NK cells can recognize and damage porcine endothelial cells. In addition, human T cells can respond to porcine endothelial cells through both direct and indirect mechanisms. Cellular rejection of porcine tissues requires T cells, particularly CD4^(+) cells. A new cytokine recombinant human interleukin-32α,β(IL-32α,β) has a role innate and acquired immune system. In order to investigate the role of recombinant mouse IL-18 and recombinant human IL-32α,β in xenograft rejection, we transplanted the PK(15) cells to C57BL/6 mice with or without intraperitoneal injection of recombinant mouse IL-18 or recombinant human IL-32 α,β. It was analyzed the population of NK cell, T cell and B cell in the C57BL/6 mice transplanted with PK(15) cells and recombinant mouse IL-18 or recombinant human IL-32α,β by flow cytometry analysis. As a result, lymph node and thymus of PK15/IL18, PK15/IL32α and PK15/IL32β injected group were increased to T cell activation population than normal injected groups. CD8^(+) T cells were decreased in lymph node of PK15/IL18, PK15/IL32α and PK15/IL32β injected groups. CD4^(+) T cells were increased in lymph node cell of PK15/IL32α and PK15/IL32β injected group and also, B cell population were increased in lymph node cell and spleen of PK15/IL18, PK15/IL32α and PK15/IL32β injected group. Therefore, we suggest that recombinant mouse IL-18 and recombinant human IL-32α,β suppress xenograft rejection in cellular xenotransplantation.

계작지모가우슬탕(桂芍知母加牛膝湯) 약침이 류마티스 관절염 생쥐에 미치는 영향

정순현 ( Soon Hyun Jung ),조종관 ( Chong Kwan Cho ),김소연 ( So Yun Kim ),김영일 ( Young Il Kim ) 대전대학교 한의학연구소 2016 혜화의학회지 Vol.24 No.2

Objectives : The purpose of this study is to prove the effect and mechanism of Gamikyejakjimogawusul-tang(GKHA) herbal acupuncture on induced rheumatoid arthritis model of DBA/1 mice. Methods : We check effect of GKHA extract on the AST, ALT, Creatinine, BUN of serum and cell viability of GK extract in RAW 264.7 cells to test the stability of this study. In vitro, we measure total phenol contents, total flavonoid contents, DPPH free radical scavenging activity, ABTS cation radical scavenging activity of Gamikyejakjimogawusul-tang, effect of GK extract on ROS(Reactive Ooxygen Species) production to estimate a anti-oxidant capacity, and we also measure effect of GK extract on NO (Nitric Oxid), IL-1β, IL-6, IL-17, IL-21, TNF-α, MCP-1, GM-CSF production in RAW 264.7 cells to estimate a anti-inflammatory efficacy. In vivo, we compare a rheumatoid arthritis manifestation between control and experimental group and estimate a AI. Then we check effect of GKHA on the level of WBC, neutrophil, lympocyte, monocyte in the blood to see the effect of immune cells in blood. In addition we measure effect of GKHA on the level of hs-CRP, IgM, IgG, IL-1β, IL-6, IL-17, IL-21, TNF-α, MCP-1, GM-CSF in serum. We observe effects of GKHA on imaging of cartilage degeneration using micro CT-arthrography in paw hind. And we calculate effects of GKHA that reduced BV ratio, BS/BV ratio using 3D Micro-CT. Lastly we observe effects of GKHA histopathologic examination analysis. Results : 1. The toxicity on liver and kidney was disregardable and the cytotoxicity against RAW 264.7 cells was also disregardable. < In vitro > 1. Total phenol contents and total flavonoid contents in GK extract were in high level. 2. DPPH free radical scavenging activity and ABTS cation radical scavenging activity were increased according to concentration of GK extract 3. ROS production was significantly decreased in GK extract (at 10, 100 ㎍/ml). 4. NO, IL-6, TNF-α, MCP-1 production were significantly decreased in GK extract(at 10, 100 ㎍/ml). IL-17, GM-CSF production were significantly decreased in GK extract(at 1, 10, 100 ㎍/ml). IL-1β, IL-21 production were also decreased but there was no statistical significance. 5. 25x observation after H&E and M-T staining, infiltration of immune cells and subsidence of the cartilage and damage to the synovial cells were decreased. Conclusions : This study showed that GKHA extract had anti-oxidant capacity, anti-inflammatory efficacy. GKHA extract also had inhibiting effect on the process of rheumatoid arthritis and can protect joint and cartilage. So we expect that GKHA extract can be a meaningful treatment to rheumatoid arthritis patients.

Interleukin-6가 사람 골수기질세포의 Alkaline Phosphatase, Osteontin, Decorin 및 a1(1)-Collagen mRNA 발현에 미치는 영향

김동관,김철희,김기수,손광현,박승일 대한내분비학회 1996 Endocrinology and metabolism Vol.11 No.2

Background: Inter1eukin-6(IL-6) is known to be produced by osteoblastic cells and to have impartant role in regulation of bone remodelling, Most previous studies indicated that IL-6 bas a major role in stimulating osteoclastic resorption by increasing recruitment and proliferation of preosteoclasts. But its autocrine effect on osteoblastic cells has not been well established yet. Therefore, we studied the effects of IL-6 on messenger RNA (mRNA) expression of proteins that are characteristic of osteoblastic cells in human bone marrow stromal (osteoprogenitor) cells (hRMSC). Methods: The expression of mRNAs for alkaline phosphatase, al(1)-collagen, osteopontin and decorin were studied by northern blot analysis after 3 7 days' treatrnent with IL-6 in the concenttation range of 101,000 U/ml. Results: The mRNA levels for any of the osteoblastic proteins studied did not change significantly by IL-6 treatment up to the concentration of 1,000 U/ml. Conclusion: These results suggest that IL-6 does not have a significant role in differentiatian or activities of human bone rnarrow stromal (osteoprogenitor) cells (J Kor Soc Endocrinol ll:156 162, 1996).

괴화 추출물이 대식세포에서의 nitric oxide와 interleukin-6의 생성에 미치는 영향

이지은,이주연,최점일,김종관,김성조,Lee, Ji-Eun,Lee, Ju-Youn,Choi, Jeom-Il,Kim, Chong-Kwan,Kim, Sung-Jo 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.1

Both nitric oxide (NO) and interleukin-6 (IL-6) have been thought to have a role in the pathogenesis of inflammatory periodontal disease as it does in other inflammatory diseases, and the inhibitors of NO and IL-6 production have been considered as potential anti-inflammatory agents. In this study, we evaluated methanol extract of Sophorae Flos for inhibition of NO and IL-6 production in Prevotella intermedia LPS-induced mouse macrophages RAW264.7 cells. Dried Sopharae Flos was sliced, and extracted with 100% methanol. LPS from p. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supematants and IL-6 was measured using mouse IL-6 ELISA kit. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. The methanol extract of Sophorae Flos concentration-dependently reduced the production of NO and the expression of iNOS protein and mRNA in RAw264.7 cells treated with P. intermedia LPS. Sophorae Flos also suppressed IL-6 production and the expression of IL-6 mRNA in RAw264.7 cells stimulated by P. intermedia LPS. The inhibition of NO and IL-6 production by Sophorae Flos may be useful in the therapy of inflammatory diseases such as periodontitis. This hypothesis, however, remains to be tested.

방풍통성산이 흰쥐의 Adjuvant 유발 관절염에 미치는 영향

엄관준 ( Kwan Joon Eom ),김지영 ( Ji Young Kim ),김순중 ( Soon Joong Kim ),서일복 ( Il Bok Seo ),정수현 ( Su Hyeon Jeong ) 한방재활의학과학회 2012 한방재활의학과학회지 Vol.22 No.2

Objectives :This study was carried out to investigate the effects of Bangpungtongseong-san(Fangfengtongsheng-san) treatment on the change of the adjuvant-induced arthritis in rats. Methods :Arthritis was induced by injection of complete Freund`s adjuvant(CFA, 0.1 ml) into the tail base of rats. Arthritic rats were divided into control(n=8) and treated(n=8) group. The control group was taken distilled water and the treated group, extracts of Bangpungtongseong-san(Fangfengtongsheng-san) by orally for 15 days. At the end of the experiment(28 days after CFA injection), gross and histopathological examinations on the articular structures of ankle joints were performed. The total number of white blood cells was measured by the ADVIA(automated blood cell counter, Bayer Co., Ireland). And also, tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) contents in paw exudate of rats were measured by enzyme-linked immunosorbent assay(ELISA) method. Results :1. Body weight(g) gain rate(%) of the treated group was increased significantly compared with control group at the 15th day after injection. 2. The thickness of ankle joints of the treated group was decreased significantly compared with the control group. 3. Histopathologically, finding of fibrous ankylosis of the treated group was decreased compared with the control group. 4. The total number of white blood cells in the treated group was decreased significantly compared with the control group. 5. TNF-α content and IL-1β content in paw exudate of the treated group were decreased significantly compared with the control group. Conclusions :On the basis of these results, we suggest that Bangpungtongseong-san(Fangfengtongsheng-san) has inhibiting effects on the progression of arthritis in CFA-induced arthritis model.

Effects of Aralia cordata Thunb. on Proteoglycan Release, Type II Collagen Degradation and Matrix Metalloproteinase Activity in Rabbit Articular Cartilage Explants

Baek, Yong-Hyeon,Seo, Byung-Kwan,Lee, Jae-Dong,Huh, Jeong-Eun,Yang, Ha-Ru,Cho, Eun-Mi,Choi, Do-Young,Kim, Deog-Yoon,Cho, Yoon-Je,Kim, Kang-Il,Park, Dong-Suk The Korean AcupunctureMoxibustion Medicine Society 2005 대한침구의학회지 Vol.22 No.2

Background & Objective: Articular cartilage is a potential target for drugs designed to inhibit the activity of matrix metalloproteinases (MMPs) to stop or slow the destruction of the proteoglycan and collagen in the cartilage extracellular matrix. The purpose of this study was to investigate the effects of Aralia cordata Thunb. in inhibiting the release of glycosaminoglycan (GAG), the degradation of collagen, and MMP activity in rabbit articular cartilage explants. Methods : The cartilage-protective effects of Aralia cordata Thunb. were evaluated by using glycosaminoglycan degradation assay, collagen degradation assay, colorimetric analysis of MMP activity, measurement of lactate dehydrogenase activity and histological analysis in rabbit cartilage explants culture. Results : Interleukin-la (IL-1a) rapidly induced GAG, but collagen was much less readily released from cartilage explants. Aralia cordata Thunb. significantly inhibited GAG and collagen release in a concentration-dependent manner. Aralia cordata Thunb. dose-dependently inhibited MMP-3 and MMP-13 expression and activities from IL-1a-treated cartilage explants cultures when tested at concentrations ranging from 0.02 to 0.2 mg/ml. Aralia cordata Thunb. had no harmful effect on chondrocytes viability or cartilage morphology in cartilage explants. Histological analysis indicated that Aralia cordata Thunb. reduced the degradation of the cartilage matrix compared with that of IL -1a-treated cartilage explants.

Effects of Aralia cordata Thunb. on Proteoglycan Release, Type II Collagen Degradation and Matrix Metalloproteinase Activity in Rabbit Articular Cartilage Explants

Baek, Yong-Hyeon,Seo, Byung-Kwan,Lee, Jae-Dong,Huh, Jeong-Eun,Yang, Ha-Ru,Cho, Eun-Mi,Choi, Do-Young,Kim, Deog-Yoon,Cho, Yoon-Je,Kim, Kang-Il,Park, Dong-Suk The Korean Acupuncture Moxibustion Medicine Societ 2005 대한침구의학회지 Vol.33 No.4

Background & Objective: Articular cartilage is a potential target for drugs designed to inhibit the activity of matrix metalloproteinases (MMPs) to stop or slow the destruction of the proteoglycan and collagen in the cartilage extracellular matrix. The purpose of this study was to investigate the effects of Aralia cordata Thunb. in inhibiting the release of glycosaminoglycan (GAG), the degradation of collagen, and MMP activity in rabbit articular cartilage explants. Methods : The cartilage-protective effects of Aralia cordata Thunb. were evaluated by using glycosaminoglycan degradation assay, collagen degradation assay, colorimetric analysis of MMP activity, measurement of lactate dehydrogenase activity and histological analysis in rabbit cartilage explants culture. Results : Interleukin-la (IL-1a) rapidly induced GAG, but collagen was much less readily released from cartilage explants. Aralia cordata Thunb. significantly inhibited GAG and collagen release in a concentration-dependent manner. Aralia cordata Thunb. dose-dependently inhibited MMP-3 and MMP-13 expression and activities from IL-1a-treated cartilage explants cultures when tested at concentrations ranging from 0.02 to 0.2 mg/ml. Aralia cordata Thunb. had no harmful effect on chondrocytes viability or cartilage morphology in cartilage explants. Histological analysis indicated that Aralia cordata Thunb. reduced the degradation of the cartilage matrix compared with that of IL -1a-treated cartilage explants.

Effects of Chung-Pae Inhalation Therapy on a Mouse Model of Chronic Obstructive Pulmonary Disease

Hwang, Joon-Ho,Lee, Beom-Joon,Jung, Hee Jae,Kim, Kwan-Il,Choi, Jun-Yong,Joo, Myungsoo,Jung, Sung-Ki Hindawi Publishing Corporation 2015 Evidence-based Complementary and Alternative Medic Vol.2015 No.-

<P>Chung-pae (CP) inhalation therapy is a method frequently used in Korea to treat lung disease, especially chronic obstructive pulmonary disease (COPD). This study investigated the effects of CP inhalation on a COPD animal model. C57BL/6 mice received porcine pancreatic elastase (PPE) and lipopolysaccharide (LPS) alternately three times for 3 weeks to induce COPD. Then, CP (5 or 20 mg/kg) was administered every 2 h after the final LPS administration. The effect of CP was evaluated by bronchoalveolar lavage (BAL) fluid analysis, histological analysis of lung tissue, and reverse transcription polymerase chain reaction analysis of mRNA of interleukin- (IL-) 1<I>β</I>, tumor necrosis factor- (TNF-) <I>α</I>, IL-6, and tumor growth factor- (TGF-) <I>β</I>. Intratracheal CP administration reduced the number of leukocytes and neutrophils in BAL fluid, inhibited the histological appearance of lung damage, and decreased the mRNA levels of the proinflammatory cytokines IL-1<I>β</I>, TNF-<I>α</I>, IL-6, and TGF-<I>β</I>. Intratracheal CP administration effectively decreased the chronic inflammation and pathological changes in a PPE- and LPS-induced COPD mouse model. Therefore, we suggest that CP is a promising strategy for COPD.</P>

하악골 충격시 안면 두개골의 응력분산양상에 미치는 구강보호장치의 역할에 관한 유한요소법적 연구

김일한,노관태,노현식,김지연,우이형,Kung-Rock Kwon,최대균 대한치과보철학회 2012 대한치과보철학회지 Vol.50 No.1

연구 목적: 이 연구의 목적은 하악골 충격 시 안면 두개골의 응력 분산양상에 미치는 구강보호장치의 효과에 대해 조사하는 것이다. 연구 재료 및 방법:: 구강보호장치를 제작하고 사람의 머리부위와 치열의 3차원적 유한요소 모델을 컴퓨터 토모그래피를 사용하여 제작하였다. 머리부위의 finite element model은 356,092요소와, 87,099절점으로 이루어져 있다. 그리고 skull과 maxillae, mandible, articular disc, teeth, 그리고 구강보호장치로 구성되었다. 경부의 움직임을 묘사하기 위하여 스프링이 사용되었다. 하악골의 충격점은 gnathion, center of inferior border 와 anterior edge of gonial angle이었다. 충격방향은 수직, 경사방향(45 도), 그리고 수평이다. 충격량은 0.1초당 800 N이었다. 결과: 수직충격을 가한 경우에는 구강보호장치의 장착여부와 무관하게 비슷한 응력과 분산양상이 나타났다(P>.05). 경사충격(45 도)을 가한 경우 구강보호장치를 장착한 모델에서는 응력이 치아와 안면골 및 두개골로 넓게 분산되었으나 이에 비하여 장착하지 않은 모델에서는 치아에 응력이 집중되었다(P<.05). 수평충격을 가한 경우 구강보호 장치를 장착한 모델에서는 응력이 치아와 안면골 및 두개골로 넓게 분산되었으나 이에 비하여 장착하지 않은 모델에서는 치아에 응력이 집중되었다(P<.05). 구강보호장치를 장착하지 않은 모델에서는 상악 치아에 응력이 집중되는 반면, 장착한 모델에서는 모든 충격실험에서 계측된 응력이 매우 낮았으며, 전달된 응력이 상악 치아와 안면골 및 두개골로 넓게 분산되었다. 결론: 구강보호장치는 외부충격 시에 하악에 수직으로 가해지는 충격에는 완충효과가 적었고, 45 도 경사 충격과 수평 충격에는 발생하는 응력을 안면골과 두개골의 넓은 범위로 분산시키고 응력을 감소시켜 응력의 완충 효과가 있었다. PURPOSE. The purpose of this study was to investigate the effects of a mouthguard on stress distribution under mandibular impact. MATERIALS AND METHODS. The FEM model of head consisted of skull, maxilla, mandible, articular disc, teeth, and mouthguard. The impact locations on mandible were gnathion, the center of inferior border, and the anterior edge of gonial angle. And the impact directions were vertical, oblique (45 degrees), and horizontal. The impact load was 800 N for 0.1 sec. RESULTS. When vertical impact was applied, the similar stress and the distribution pattern was occurred without the relation of the mouthguard use (P>.05). The model with mouthguard was dispersed the stress to the teeth, the facial bone and the skull when the oblique (45 degrees) impacts were happened. However, the stress was centralized on the teeth in the model without mouthguard (P<.05). The model with mouthguard was dispersed the stress to the teeth, the facial bone and the skull when the horizontal impacts was occurred. However, the stress was centralized on the teeth without mouthguard (P<.05). For all impact loads, stress concentrated on maxillary anterior teeth in model without mouthguard, on the contrary, the stress was low in the model with mouthguard and distributed broadly on maxillary anterior teeth, facial bone, and skull. CONCLUSION. The mouthguard was less effective at shock absorbing when vertical impact was added. However, it was approved that mouthguard absorbed the shock regarded to the oblique (45 degrees) and horizontal impact by dispersing the shock to the broader areas and decreasing the stress.

한국 개화기 신민회의 교육구국 사상

한관일 청주대학교 교육문제연구소 2000 교육과학연구 Vol.14 No.-

The purpose of this study in to examine the aspect how much influence the save-the-netion thorough education by the society Sin-Min, how much meaningful it is now. this study is based mainly by the documentary mateials from the point of national resistans history. Ducumentary materials include Dae-Han daily newspaper, Whang-Seong newspaper, Kwan-Bo, and Academic journals(學會誌). The scope and subject of this study were focussed on the national private schools astablished by the society Sin-Min in late Chosun, namely from 1905 to 1910. The content of this is summarized as follows. The Japanese government schemed policy for ignorance and policy for assimilation in order to illiterate and Japanize the Koreans. Against the Japanese suppression, Koreans developed many-sided anti-Japanese movement, the national salvation movement and the save-the-nation movement through education by the society Sin-Min is summerized as follows (1) Inspiration of patriotic and independence thought (2) The recovery of rights (3) Acceptance of the new education system (4) Emphasis of mental, moral and physical education. The save-the-nation movement through education by the society Sin-Min was put into practice by the education. through the patriotic textbooks, the militant physical training, the national folk-songs education and the teacher training education. As stated above, the national the private schools by the society Sin-Min becamed the center of the modern independence movement and national education activites of Korea. The save-the-nation thought education by the society Sin-Min highly valued to develop the modorn education by the ideology of education and the reoganization of eduation environment.