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Mutation analysis and characterisation of F9 gene in haemophilia- B population of India
Sujayendra Kulkarni,Rajat Hegde,Smita Hegde,Suyamindra S. Kulkarni,Suresh Hanagvadi,Kusal K. Das,Sanjeev Kolagi,Pramod B. Gai,Rudragouda Bulagouda 대한혈액학회 2021 Blood Research Vol.56 No.4
Background Hemophilia B (HB) is an X-linked bleeding disorder resulting from coagulation factor IX defects. Over 3,000 pathogenic, HB-associated mutations in the F9 gene have been identified. We aimed to investigate the role of F9 variants in 150 HB patients using sequencing technology. Methods F9 gene sequences were amplified from peripheral blood-derived DNA and sequenced on an Applied Biosystems (ABI) 3500 Sanger sequencing platform. Functional and structural predictions of mutant FIX were analyzed. Results Among 150 HB patients, 102 (68%), 30 (20%), and 18 (12%) suffered from severe, moderate, and mild HB, respectively. Genetic analysis identified 16 mutations, including 3 novel mutations. Nine mutations (7 missense and 2 stop-gain) were found to be pathogenic. Only 3 mutations (c.127C>T, c.470G>A, and c.1070G>A) were associated with different severities. While 2 mutations were associated with mild HB cases (c.304C>T and c.580A>G), 2 (c.195G>A and c.1385A>G) and 3 mutations (c.223C>T, c.1187G>A, and c.1232G>A) resulted in moderate and severe disease, respectively. Additionally, 1 mutation each was associated with mild-moderate (c.*1110A>G) and mild-severe HB disease (c.197A>T), 4 mutations were associated with moderate-severe HB cases (c.314A>G, c.198A>T, c.676C>T, and c.1094C>A). FIX concentrations were lower in the mutated group (5.5±2.5% vs. 8.0±2.5%). Novel p.E66D and p.S365 mutations were predicted to be pathogenic based on changes in FIX structure and function. Conclusion Novel single nucleotide polymorphisms (SNPs) largely contributed to the pathogenesis of HB. Our study strongly suggests that population-based genetic screening will be particularly helpful to identify risk prediction and carrier detection tools for Indian HB patients.
Bioremediation of imidacloprid using Azospirillium biofertilizer and Rhizobium biofertilizer
Kavita Kulkarni,Aishwarya Chawan,Anand Kulkarni,Sandip Gharat 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.10
Imidacloprid is a pesticide used for agricultural purposes. Residue of pesticide in water and soil will affect the water and soil quality. Seepage out of imidacloprid to the ecological system could affect aquatic life as well as human. The toxic pollutants would affect the quality of agriculture run off, in turn contaminating water bodies acting as sink for these runoffs. Hence, there is need for reparation of these pollutants. Azospirillium biofertilizer and Rhizobium biofertilizer were used as adsorbent for the removal of imidacloprid. These biofertilizers have capability to reduce the harmful component as well as be useful for plant growth. Azospirillium bacteria and Rhizobium bacteria are competent for the removal of organic pollutant from wastewater. These biofertilizers maintain biological activity without any adverse effect. The adsorptive removal of imidacloprid by using Azospirillium biofertilizer and Rhizobium biofertilizer was investigated at different conditions using batch experimentation. Optimization of parameters, such as dosage, time, temperature, pH, and agitation speed, was carried out. Equilibrium adsorption was illustrated by Langmuir and Freundlich isotherms. The kinetic data was best described by intraparticle diffusion and pseudo-second-order model. Reusability study showed good removal efficiency of imidacloprid after fourth use also. The investigations show that these materials have potential to be an excellent alternative for removal of pesticides while supporting plant growth.
Kulkarni, Raghavendra Narayanrao,Baskaran, Kuppusamy 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.2
An extremely tall mutant (EMS 18-12), a bushy mutant (EMS 24-5), their parental variety Nirmal, and their double mutant recombinant were used to study individual and combined effects of genes producing opposite effects on plant height in periwinkle (Catharanthus roseus). Plant height of the extremely tall mutant (EMS 18-12) was controlled by epistatic inhibitory interaction between two independently inherited dominant genes, Et and H; Et producing extremely tall phenotype and H inhibiting Et. Both genes were inherited independently of plant height-reducing recessive gene by in the bushy mutant (EMS 24-5). Individually, genes Et and by increased and reduced plant height at harvest (when plant were 9 months old) by 90 and 25%, respectively, over parental variety. The double mutant recombinant (Etby) was taller than the bushy mutant (EMS 24-5) and variety, Nirmal but shorter than the extremely tall mutant (EMS 18-12) at different stages. At 1 to 7 weeks after germination, its height was 7.4 to 30.0% greater than the mid-parental value but 5.8 to 30.5% shorter than that expected on the basis of individual effects of genes Et or by. At the age of 4 to 9 months, its height was 5.4 to 40.1% greater than the mid-parental value and 5.6 to 44.1% (except at 5 months) greater than that expected on the basis of individual effects of genes Et or by, suggesting age-dependent epistatic interaction between the genes. No interaction effects were observed for leaf and root yields or contents of alkaloids in leaves and roots.
Kulkarni, Sunita M.,Sundari, G. International Journal of Computer ScienceNetwork S 2021 International journal of computer science and netw Vol.21 No.2
Glioma is one of the common types of brain tumors starting in the brain's glial cell. These tumors are classified into low-grade or high-grade tumors. Physicians analyze the stages of brain tumors and suggest treatment to the patient. The status of the tumor has an importance in the treatment. Nowadays, computerized systems are used to analyze and classify brain tumors. The accurate grading of the tumor makes sense in the treatment of brain tumors. This paper aims to develop a classification of low-grade glioma and high-grade glioma using a deep learning algorithm. This system utilizes four transfer learning algorithms, i.e., AlexNet, GoogLeNet, ResNet18, and ResNet50, for classification purposes. Among these algorithms, ResNet18 shows the highest classification accuracy of 97.19%.
Kulkarni, Krishnanand P.,Asekova, Sovetgul,Lee, Dong-Ho,Bilyeu, Kristin,Song, Jong Tae,Lee, Jeong-Dong CSIRO Publishing 2017 Crop & pasture science Vol.68 No.2
<P> Seed weight can be an important component for soybean quality and yield. The objective of the present study was to identify quantitative trait loci (QTLs) for 100-seed weight by using 169 recombinant inbred lines (RILs) derived from the cross Williams 82 × PI 366121. The parental lines and RILs were grown for four consecutive years (2012-15) in the field. The seeds were harvested after maturity, dried and used to measure 100-seed weight. Analysis of variance indicated significant differences among the RILs for 100-seed weight. The environment had significant effect on seed-weight expression as indicated by the genotype × environment interaction. QTL analysis employing inclusive composite interval mapping of additive QTLs implemented in QTL IciMapping (Version 4.1) identified nine QTLs (LOD >3) on chromosomes 1, 2, 6, 8, 13, 14, 17 and 20. The individual QTLs explained phenotypic variation in the range 6.1-12.4%. The QTLs were detected in one or two environments, indicating major influence of the growing environment on seed-weight expression. Four QTLs identified in this study, qSW-02_1, qSW-06_1, qSW-13_1 and qSW-14_1, were found to be new QTLs. The findings of the study may be helpful to reveal the molecular genetic basis of the seed-weight trait in soybean. </P>
Kulkarni GT,Gowthanarajan K,Raghu C,Ashok G,Vijayan P Kyung Hee Oriental Medicine Research Center 2005 Oriental pharmacy and experimental medicine Vol.5 No.1
Polysaccharide hydrogels constitute a structurally diverse class of biological macromolecules with a wide range of physicochemical properties. They also constitute important members of the family of industrial water-soluble polymers. They find application in Pharmacy as binders, disintegrants, suspending, emulsifying and sustaining agents. According to the International Pharmaceutical Excipients Council (IPEC), an excipient must have an established safety profile. Hence, in the present study, in vitro cytotoxicity on Vero and HEp-2 cell lines, and in vivo acute toxicity in rats were carried out to establish the safety of polysaccharide hydrogels from the seeds of Plantago ovata and Ocimum basilicum. The in vitro cytotoxicity was determined by MTT and SRB assays. In the in vivo acute toxicity, the effects of three different doses of hydrogels (100, 200 and 400 mg/kg body weight) on food and water intake, body weight, biochemical and hematological parameters were studied. The results of in vitro did not show any cytotoxicity on both the cell lines used. In the in vivo acute toxicity, the hydrogels did not show any toxic symptoms in all three dose levels. This establishes the safety of the selected hydrogels. Hence, they can be used as excipients in pharmaceutical dosage forms.
Kulkarni, Ashwini N.,Watharkar, Anuprita D.,Rane, Niraj R.,Jeon, Byong-Hun,Govindwar, Sanjay P. Elsevier 2018 Ecotoxicology and environmental safety Vol.148 No.-
<P>Navy Blue HE22 (NBHE22), dye mixture and real textile effluent were decolorized and degraded by lichen Derrnatocarpon vellereceuen. Up-flow bioreactor showed about 80%, 70%, 80% and 65% removal of American dye manufacturer index (ADMI), biological oxygen demand (BOD), total suspended solids (TSS) and total dissolved solids (TDS), respectively of dye mixture at flow rate of 25 ml h(-1). The removal of ADMI, BOD, TSS and TDS of real textile effluent were 75%, 65%, 82% and 70%, respectively at flow rate of 30 ml h(-1). Significant induction of extracellular enzymes such as manganese peroxidase and lignin peroxidase was observed up to 46% and 36% during decolorization of dye mixture, while 43% and 24% during effluent treatment, respectively. Exponential enhancement in the activities of stress enzymes such as catalase (CAT) and guaiacol peroxidase (GPX) was observed after exposure to NBHE22 (116% and 125%, respectively), dye mixture (150% and 300%, respectively) and effluent (400% and 350%, respectively) endorsing the stress tolerance ability of model lichen. Phytotoxicity and genotoxicity studies demonstrated less toxic nature of metabolites resulted from biodegradation.</P>