의료 질 향상 사업의 성공요인과 실패요인

최귀선,이선희,조우현,강혜영,채유미 한국의료QA학회 2001 한국의료질향상학회지 Vol.8 No.2

Background : To propose effective strategies for successful implementation of QI in health care institutions, by identifying facilitating factors and barriers to conducting QI programs. Methods : In order to examine empirical evidence on the success factors or barriers to QI implementation in hospitals, a literature study was performed on the basis of MEDLINE search. Among the identified literature. 13 provided reliable findings and basis comprehensive discussion on this issue and thus were selected for in-depth analysis. A mailed questionnaire survey was conducted for hospital CEOs and QI directors of hospitals with 400 beds or greater to investigate what attributes of their organizations they perceived as success factors or obstacles to QI implementation. Result : The analysis of selected literature and survey results presented that the primary factors considered to be most important as successful implementation of QI were: strong support from hospital CEOs, setting higher priority for QI activities, continuous and persistent efforts in QI activities, and active participation of clinical staffs. The barriers identified in this study were : the lack of orientation and understanding of QI concepts, low level of interest and participation of physician in QI programs, the lack of evaluation and rewarding system for QI activities. Conclusion : By identifying factors that affect facilitation of QI, the study results will be of great use for either institutions being in the early stage of evolving QI or those looking for better strategies to achieve more active and persistent QI implementation in their institutions.

Classical Swine Fever (Hog cholera) Virus 약독순화주 (Suri 주)의 gp55 Gene 염기서열 분석

김귀현,장경수,강경임,이병형,박종현,안수환,전무형 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

An attenuated classical swine fever virus (CSFV), Suri strain, is a variant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal trasmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compard the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residuse in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein.

카드뮴 및 청국장 첨가식이가 흰쥐의 생육과 체내 카드뮴 분포에 미치는 영향

주현규,강경원,사동민 선문대학교·중소기업기술지원연구소 1998 선문공대 연구/기술 논문집 Vol.3 No.1

This study was performed to investigate the effect of Chungkuk Jang added-diets on reducing cadmium toxiclt in rats (Sprague-Dalvley, ♂, 125 58±4.04g) used as experimental groups. There are six experimental groups, a normal group(Tn), a cadmium treatment group(control group Tc), four cadmium and Chungkuk Jang (0.25%, 0 50%, 1 00%, and 1 50%; T_1 T_2, T_3, and T_4) treatment groups which received different dietary feeding for 8 weeks each The results are as follows. 1 The amount of food intake at Chungkuk Jang mixed group(T_1, -T_2) was 3 5% greater than at the Tc group For the group fed Chungkuk Jang the average of weight gain was 4.42g/day, which showed higher value(2.79%) than the Tc(4 30g/day) group. The food efficency rate of intake Chungkuk Jang group (5.02) was a little higher without significance than that of the Tn(4 91) and Tc(4 99) groups. 2. The amount of water intake was the highest in the Tn(32.44?/day) group and 30.14% greater than that of the Tc(21.66?/day) group. For the water intake (T_3) among Chungkuk Jang added-diet groups showed the highest value(22.78?/day) relatively Cadmium intake of each group was in the range of 1.94 to 2.27 ppm and the T_3 group showed the highest cadmium intake. For the growth rate the Tc group was 7.62% lower than Tn group but the Chungkuk jang fed group resulted in a reduced fashion-about 3% in the growth process against the Tn group 3 There was no significant weight gain or loss of the organ and bone of rats among the experimental groups but in the case of liver and kidney the T_3 (8.59%. 12 36%) was higher than the Tc group 4 Cadmium accumulation was high to low in order of liver > kidney > spleen > heart > lung > testicle > brain > femoral muscle > femur >(T_1, T_2, T_3, and T_4). tibia > blood. The 70% accumulation rate was showed in liver (33 2%), kidney (31.25) and spleen (6 1%). The contents of cadmium in tissue and body-hair in the Chungkuk Jang intake groups(T_1, T_2, T_3, and T_4) were lower than in the Tc group. The chungkuk Jang added-diet groups (T_3, and T_4) showed low levels of cadmium accumulation

Assessment of In Vitro Assay System for Thyroid HormoneDisruptors Using Rat Pituitary GH3 Cells

Hee Jin Kim1,Hae Young Park1,Jeonga Kim1,Il Hyun Kang2,Tae Sung Kim2,Soon Young Han2,Tae Seok Kang2,Kui Lea Park2,Hyung Sik Kim1 한국독성학회 2006 Toxicological Research Vol.22 No.4

The development of in vitro assays has been recommended to screening and test-ing the potential endocrine disruptors (EDs). These assay systems focus only on identifying thethe thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detectTH disruptors using rat pituitary tumor GH3 cells. The test system is based on the TH-dependentincrease in growth rate. As expected, L-3,5,3-triiodothyronine (T3) markedly induced a morphologicalchange in GH 3 cells from flattened fibroblastic types to rounded or spindle-shaped types. T3 stimu-lated GH3 cell growth in a dose-dependent manner with the maximum growth-stimulating effect9 M. In addition, T3 increased the release of growth hor-mone and prolactin into the medium of the GH3 cells culture. Using this assay system, the TH-dis-rupting activities of bisphenol A (BPA) and its related compounds were examined. BPA,dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH3 cells in therange of 1 × 10-5M to 1 × 10-6M concentrations. In conclusion, this in vitro assay system might bestandardization before it can be used as a broad-based screening tool.

Effect of Culture Conditions on the Production of Succinate by Enterococcus faecalis RKY1

Kang, Kui Hyun,Yun, Jong Sun,Ryu, Hwa Won 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.1

Bioconversion of fumarate to succinate was anaerobically conducted in a synthetic medium containing glycerol as a hydrogen donor and fumarate as a hydrogen acceptor. We investigated the effects of pH, carbon and nitrogen sources, conversion substrate, and other culture conditions on the production of succinate using a newly isolated Enterococcus faecalis RKY1. Addition of a variety of carbonates to the medium significantly increased the rates of production of succinate. The production of succinate and cell growth were relatively satisfactory in the pH range of 7.0-7.6. By using glycerol as a hydrogen donor, high purity succinate was produced with few byproducts. Yeast extract as a sole nitrogen source was the most effective for producing succinate. As a result, the optimum condition of bioconversion was obtained at a medium containing 20g/l glycerol, 50g/l fumarate, 15g/l yeast extract, 10g/l K_2HPO_4 1g/l NaCl, 50 ppm MgCl_2, .6H_2O, 10ppm FeSO_4, . 7H_2O, and 5g/l Na_2CO_3 at pH 7.0-7.6. Under the optimum condition, a succinate concentration of 153g/l was produced in 36h. The total volumetric production rate and the molar yield of succinate were 4.3g/l/h and 85%, respectively.

Enhancement of Succinate Production by Organic Solvents , Detergents , and Vegetable Oils

(Kui Hyun Kang),(Hwa Won Ryu) 한국미생물생명공학회 1999 Journal of microbiology and biotechnology Vol.9 No.2

N/A N/A

Anti-Androgenic Activity of Phthalate Esters (Di(2-ethylhexyl) Phthalate, Di(n-butyl) Phthalate, and Butylbenzyl Phthalate) in the Rodent 10-day Hershberger Assay using Immature Castrated Male Rats

Kang, Il-Hyun,Kim, Hyung-Sik,Kim, Tae-Sung,Moon, Hyun-Ju,Kim, In-Young,Kang, Tae-Seok,Park, Kui-Lea,Choi, Kwang-Sik,Han, Soon-Young Korean Society of ToxicologyKorea Environmental Mu 2005 Toxicological Research Vol.21 No.3

The rodent Hershberger assay is considered as a potential short term in vivo screening method for the detection of androgenic or anti-androgenic compounds. The objective of this study was to evaluate the anti-androgenic activities of di(2-ethylhexyl) phthalate (DEHP), di(n-butyl) phthalate (DBP), and butylbenzyl phthalate (BBP). A 10-day Hershberger assay was performed using immature Sprague-Dawley male rats castrated at 6 weeks of age. Tastosterone propionate (TP, 0.4 mg/kg/day) was administered s.c. to castrated male rats and followed by flutamide (1, 5, 10, or 20 mg/kg/day) treatment for 10 days by oral gavage. Similarly, DEHP, DBP, or BBP were also administered by oral gavage at 250, 500, or 1000 mg/kg/day after TP (0.4 mg/kg/day) administration. As expected, flutamide significantly inhibited the TP-induced re-growth of seminal vesicles, ventral prostate, and Levator ani plus bulbocavernosus muscles (LABC) at 1 mg/kg/day and above, and Cowper's glands and glans penis at 5 mg/kg/day and above. DEHP significantly (p<0.05) decreased the seminal vesicles, ventral prostate, LABC and Cowper's glands weights at 1000 mg/kg/day. BBP at 1000 mg/kg/day significantly inhibited TP-induced re-growth of the LABC in the immature castrated male rats, whereas ventral prostate, seminal vesicles, and Cowper's glands weights were unaffected. In contrast to DEHP, DBP did not affect accessory sex organ weights at any concentration. Body weights, combined adrenal glands, and kidney weights were not affected, but liver weights were significantly increased at high dosages in the DEHP, DBP, and BBP treatment groups. Our observations strongly suggest that DEHP acts as an androgen antagonist at the high dose (i.e., 1000 mg/kg/day).

Anaerobiospirillum succiniciproducens에 의한 유기산 생성에 미치는 pH의 영향

강귀현(Kui-Hyun Kang),류화원(Hwa-Won Ryu),장호남(Ho-Nam Chang) 한국생물공학회 1995 KSBB Journal Vol.10 No.5

Anti-Androgenic Activity of Phthalate Esters (Di(2-ethylhexyl) Phthalate, Di(n-butyl) Phthalate, and Butylbenzyl Phthalate) in the Rodent 10-day Hershberger Assay using Immature Castrated Male Rats

Il Hyun Kang,Hyung Sik Kim,Tae Sung Kim,Hyun Ju Moon,In Young Kim,Tae Seok Kang,Kui Lea Park,Kwang Sik Choi,Soon Young Han 한국독성학회 2005 Toxicological Research Vol.21 No.3

The rodent Hershberger assay is considered as a potential short term in vivo screening method for the detection of androgenic or anti-androgenic compounds. The objective of this study was to evaluate the anti-androgenic activities of di (2-ethylhexyl) phthalate (DEHP), di (n-butyl) phthalate (DBP), and butylbenzyl phthalate (BBP). A 10-day Hershberger assay was performed using immature Sprague-Dawley male rats castrated at 6 weeks of age. Testosterone propionate (TP, 0.4 ㎎/㎏/day) was administered s.c. to castrated male rats and followed by flutamide (1, 5, 10, or 20 ㎎/㎏/day) treatment for 10 days by oral gavage. Similarly, DEHP, DBP, or BBP were also administered by oral gavage at 250, 500, or 1000 ㎎/㎏/day after TP (0.4 ㎎/㎏/day) administration. As expected, flutamide significantly inhibited the TP-induced re-growth of seminal vesicles, ventral prostate, and Levator ani plus bulbocavernosus muscles (LABC) at 1 ㎎/㎏/day and above, and Cowper’s glands and glans penis at 5 ㎎/㎏/day and above. DEHP significantly (p < 0.05) decreased the seminal vesicles, ventral prostate, LABC and Cowper’s glands weights at 1000 ㎎/㎏/day. BBP at 1000 ㎎/㎏/day significantly inhibited TP-induced re-growth of the LABC in the immature castrated male rats, whereas ventral prostate, seminal vesicles, and Cowper’s glands weights were unaffected. In contrast to DEHP, DBP did not affect accessory sex organ weights at any concentration. Body weights, combined adrenal glands, and kidney weights were not affected, but liver weights were significantly increased at high dosages in the DEHP, DBP, and BBP treatment groups. Our observations strongly suggest that DEHP acts as an androgen antagonist at the high dose (i.e., 1000 ㎎/㎏/day).

Durable nanocomposite adhesives based on polyacrylates and surface-modified colloidal silica

Kang, Young Taec,Hwang, Kui Seung,Moon, Jin-Bok,Kim, Dong-Hyun,Chung, Ildoo VNU Science Press 2015 Journal of Adhesion Science and Technology Vol. No.