http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Uzma Qaisar,Cassandra J. Kruczek,Muhammed Azeem,Nasir Javaid,Jane A. Colmer-Hamood,Abdul N. Hamood 한국미생물학회 2016 The journal of microbiology Vol.54 No.8
Proteins encoded by the Pseudomonas aeruginosa pvcA-D operon synthesize a novel isonitrile functionalized cumarin termed paerucumarin. The pvcA-D operon enhances the expression of the P. aeruginosa fimbrial chaperone/usher pathway (cup) genes and this effect is mediated through paerucumarin. Whether pvcA-D and/or paerucumarin affect the expression of other P. aeruginosa genes is not known. In this study, we examined the effect of a mutation in pvcA-D operon the global transcriptome of the P. aeruginosa strain PAO1- UW. The mutation reduced the expression of several ironcontrolled genes including pvdS, which is essential for the expression of the pyoverdine genes. Additional transcriptional studies showed that the pvcA-D operon is not regulated by iron. Exogenously added paerucumarin enhanced pyoverdine production and pvdS expression in PAO1-UW. Iron-chelation experiments revealed that purified paerucumarin chelates iron. However, exogenously added paerucumarin significantly reduced the growth of a P. aeruginosa mutant defective in pyoverdine and pyochelin production. In contrast to other secondary metabolite, Pseudomonas quinolone signal (PQS), paerucumarin is not localized to the P. aeruginosa membrane vesicles. These results suggest that paerucumarin enhances the expression of iron-controlled genes by chelating iron within the P. aeruginosa extracellular environment. Although paerucumarin chelates iron, it does not function as a siderophore. Unlike PQS, paerucumarin is not associated with the P. aeruginosa cell envelope.
Olga Długosz-Grochowska,Renata Wojciechowska,Michał Kruczek,Anna Habela 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.5
In recent years, light-emitting diodes (LEDs) have been introduced as a new source of supplementallighting in winter greenhouse crop production to improve yield and quality. The aim of this study was to determinethe effect of light supplementation with LEDs on the yield and the concentration of several health-promotingcompounds in two cultivars of lamb’s lettuce (Valerianella locusta, L.), ‘Noordhollandse’ and ‘Holländisher’ in agreenhouse cultivation in winter. Four different LED spectra used to extend the photoperiod (16 h day/8 h night)were tested: 10R:0B (100% red), 9R:1B (90% red, 10% blue), 8R:2B (80% red, 20% blue), and 7R:3B (70% red,30% blue). The control plants were illuminated by high-pressure sodium (HPS) lamps. The photosynthetic photonflux density at the plant canopy level was approximately 200 μmol·m-2·s-1in all treatments. The plants were harvestedon the 45thday of light treatment (60 days after sowing). In both cultivars, the 10R:0B LED light treatment resultedin the highest yield, but low concentrations of ascorbic acid, xantophylls, and phenolic compounds. Supplemental lightingwith HPS lamps resulted in high concentrations of nitrate and carotenoids, but low fresh mass, ascorbic acid, andtotal phenols content. The addition of blue light in the spectrum increased antioxidant properties; however, certain effectswere cultivar-dependent. High yields with the most satisfactory composition of soluble sugars, ascorbic acid, carotenoids,and polyphenols, as well as high antioxidant properties and reduced nitrate levels, in the leaves of the tested cultivarswere obtained with 8R:2B LED lighting. Thus, supplemental lighting with 80% red and 20% blue light may be advantageousin greenhouse-cultivated V. locusta ‘Noordhollandse’ and ‘Holländisher’ cultivars.