슬러지 재자원화에 관한 연구

이주성,공성호,서승원,배성렬,김영채,이기철 漢陽大學校 環境工學硏究所 1997 環境科學論文集 Vol.18 No.-

이 연구는 1993년도에 우리나라의 연간 슬러지발생량이 약 3500만m³에 이르고 있으며, 이들 대부분이 매립되고 있다. 그러나 매립부지의 확보가 점점 어려워 지고 있으며 아울러 매립처분비용도 상승하고 있기 때문에 oil화, 퇴비화등으로 재자원화 하는 방법과 소각후의 소각회를 유효이용하는 방법 등이 연구 개발되고 실용화되고 있다. 슬러지의 oil화는 현재 연구단계에 있으나 앞으로 슬러지의 유효이용기술로서, 가장 좋은 방법이 되리라 기대되고 있다. 그러나 소각후의 소각회의 이용방법은 현재 일본등에서는 실용화 되어 건설자재 등으로 다양하게 이용하고 있다. 우리나라에서도 서울시 등을 비롯한 대도시에서는 하수처리장에서 발생하는 슬러지를 소각한 후에 소각회를 건설 자제용으로서 지자체의 공사에는 반드시 사용하도록 하는 제도를 만든다면 유효이용방법으로 충분한 타당성이 있을 것이다. 이때 소각방법으로는 용융소각방법이 보다 효과적일 것이다. With increasing sewage and wastewater sludge, it has become difficult to dispose the sludge by landfilling and ocean dumping. To solve this problem, efforts have been made to delelop sewage and wastewater sludge utilization technologies in many advanced countries. Some technologies have already been developed; conversion of sludge to compost and fuel, producing artificial light-weight aggregate from ashes. This paper describes the technical status of sludge utilization technologies.

흑연료 원자흡수 분광법에 의한 혈중의 납, 카드뮴 정량을 위한 외부정도관리 시료제조 및 분석

이공주,임홍빈 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

납과 카드늄을 포함하는 여러가지 농도의 동결건조된 혈액이 외부정도관리 시료로서 제조되었다. 이 시료들은 흑연료 원자흡수분광법(GFAAS)을 이용하여 성능이 파익되었다. 매트릭스 개선제로서 0.1% ammonium dihydrogen phosphate와 0.1% Triton X-100을 사용하여 섭씨 600 내지 650도의 회화온도에서 혈액에 있는 납과 카드늄의 정량분석을 위한 GFAAS의 최적 분석조건이 얻어졌다.제조된 혈액의 균질도와 안정도는 분석조건에서 연구되었다. Lyophilized whole blood samples containing various concentrations of Pb and Cd have been prepared as external quality control materials. These materials have been characterized with graphite furnace atomic absorption spectrometry(GFAAS). The optimized conditions for the quantitative determination of Pb and Cd in whole blood using GFAAS were obtained at the ashing temperature of 600∼650℃, with 0.1% ammonium dihydrogen phosphate and 0.1% Triton X-100 as matrix modifier. Homogeniety and stability of the prepared whole blood have been studied at the optimized analytical condition.

흑연료 원자흡수 분광법에 의한 혈중의 납, 카드뮴 정량을 위한 외부정도관리 시료제조 및 분석

이공주,임홍빈 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-

납과 카드뮴을 포함하는 여러 가지 농도의 동결건조된 혈액이 외부정도관리 시료로서 제조되었다. 이 시료들은 흑연료 원자흡수분광법(GFAAS)을 이용하여 성능이 파악되었다. 매트릭스 개선제로서 0.1% ammonium dihydrogen phosphate와 0.1% Triton X-100을 사용하여 섭씨 600내지 650도의 희화온도에서 혈액에 있는 납과 카드뮴의 정량 분석을 위한 GFAAS의 최적 분석조건이 얻어졌다. 제조된 혈액의 균질도와 안정도는 최적화된 분석조건에서 연구되었다. Lyophilized whole blood samples containing various concentrations of Pb and Cd have been prepared as external quality control materials. These materials have been characterized with graphite furnace atomic absorption spectrometry(GFAAS). The optimized obtained at the ashing temperature of 600~650℃ with 0.1% ammonium dihydrogen phosphate and 0.1% Triton X-100 as matrix modifier. Homogeniety and stability of the prepared whole blood have been studied at the optimized analytical condition.

모세관 전기영동 분석법의 복합약물제제의 품질관리 분석에 응용을 위한 연구

허유정,이공주 梨花女子大學校 藥學硏究所 1998 藥學硏究論文集 Vol.- No.7

Capillary electrophoresis (CE) is perceived as an attractive tool for the analysis of pharmaceuticals and biological materials because of their high separation efficiency. easy separation and low running cost. New concept of micellar electrokinetic capillary chromatography (MECC) expanded the application of CE to the separation of neutral molecules. Validation of CE as an analytical technique for quality control of pharmaceuticals should be confirmed by quantitative analysis and the peak confirmation. In this study, the quantitative analyses of various types of neutral. acidic and basic components (acetaminophen. caffeine. ascorbic acid. riboflavin. thiamine. chlorpheniramine. phenylpropanolamine. dl-methylephedrine and dextromethorphan) in complex cold medicines have been accomplished using CE. Combined methods of MECC using SDS and capillary zone electrophoresis lowering the pH of running buffer were adopted to determine the ingredients in capsule type or liquid formula complex medicines without particular sample pretreatment. The results indicate that CE is a promising technique for quality control analysis of pharmaceuticals as a validation method.

ROSPath : An Intergrated Database for Reactive Oxygen Species-mediated Signaling Pathway

Lee, Kong-Joo,Paek, Eunok,Kim, Hee-Jung,Yang, Kap-Seok,Lee, Sanghyuk,Lee, Seung-Rock,Choi, Kiyoung,Park, Jisook 이화여자대학교 세포신호전달연구센터 2004 고사리 세포신호전달 심포지움 Vol. No.6

Progress in understanding complex signaling pathways and networks has been hampered by the current lack of a formal and structured collation of the available information, in a format suitable for analysis via software tools. In order to facilitate the organization. and understanding of such complex network of information, it is essential to have a formal means to represent and analyze cellular pathways. We have defined a formal ontology for cell-signaling events that allows us to describe these cellular pathways at various levels of abstractions. Using this formal representation, ROSPath(Reactive Oxygen Species related signaling Pathway) database system has been implemented and made available on the web(http://rospath.ewha.ac.kr). ROSPath is a database system for reactive oxygen species(ROS)-mediated cell signaling pathways and signaling processes in molecular detail, that facilitates a comprehensive understanding of the regulatory mechanisms in signaling pathways. ROSPath includes growth factor-, stress- and cytokine-induced signaling. It is a web-based structured repository of information on the signaling pathways of interest and provides a means for managing data produced by high-throughput tools such as proteomics and genomics. It also provides effective and flexible tools for querying, displaying and analyzing pathways, thus providing an integrated web environment for visualizing and manipulating ROS-mediated cell-signaling events.

Novel Oxidative Modifications in Redox-Active Cysteine Residues

Kong-Joo, Lee,Jaeho, Jeong,Yongsik, Jung,Seungjin, Na,Eunsun, Lee,Mi-Sun, Kim,Sun, Choi,Dong-Hae, Shin,Eunok, Paek,Hee-Yoon, Lee 이화여자대학교 약학연구소 2012 藥學硏究論文集 Vol.- No.22

Redox-active cysteine, a highly reactive sulfhydryl, is one of the major targets of ROS. Formation of disulfide bonds and other oxidative derivatives of cysteine including sulfenic, sulfinic, and sulfonic acids, regulates the biological function of various proteins. We identified novel low-abundant cysteine modifications in cellular GAPDH purified on 2-dimensional gel electrophoresis (2D-PAGE) by employing selectively excluded mass screening analysis for nano ultraperformance liquid chromatography-electrospray-quadrupole-time of flight tandem mass spectrometry, in conjunction with MODi and MODmap algorithm. We observed unexpected mass shifts (Δm=-16, -34, +64, +87, and +103 Da) at redox-active cysteine residue in cellular GAPDH purified on 2D-PAGE, in oxidized NDP kinase A, peroxiredoxin 6, and in various mitochondrial proteins. Mass differences of -16, -34, and +64 Da are presumed to reflect the conversion of cysteine to serine, dehydroalanine (DHA), and Cys-SO2-SH respectively. To determine the plausible pathways to the formation of these products, we prepared model compounds and examined the hydrolysis and hydration of thiosulfonate (Cys-S-SO2-Cys) either to DHA (Δm=-34 Da) or serine along with Cys-SO2-SH (Δm=+64 Da). We also detected acrylamide adducts of sulfenic and sulfinic acids (+87 and +103 Da). These findings suggest that oxidations take place at redox-active cysteine residues in cellular proteins, with the formation of thiosulfonate, Cys-SO2-SH, and DHA, and conversion of cysteine to serine, in addition to sulfenic, sulfinic and sulfonic acids of reactive cysteine.

Clinicopathologic factors of ovarian clear cell carcinoma in a background of endometriosis: Clues for proper treatment and follow up intervals

( Joo-hyuk Son ),( Hana Cho ),( Joo-hyung Lee ),( Tae-wook Kong ),( Jiheum Paek ),( Yong-hee Lee ),( Hee-sug Ryu ),( Suk-joon Chang ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: The aim of this study was to analyze clinicopathologic factors and oncologic outcomes in patients with ovarian clear cell carcinoma associated with endometriosis in order to provide clinical answers to follow up intervals and treatment strategy for endometriosis. 방법: We retrospectively reviewed clinicopathologic data of 50 patients with ovarian clear cell carcinoma between March of 2001 and March 2016. Clinicopathologic characteristics, treatment outcomes, and relations to endometriosis were analyzed. 결과: The most common initial symptom of clear cell carcinoma was palpable mass (32%) followed by incidental diagnosis during regular follow up of endometriosis without any symptom (22%). Compared to other symptomatic groups, asymptomatic patients diagnosed with clear cell carcinoma during regular gynecologic check up were more likely to have low recurrence rate (9.09% vs. 35.8%) and early FIGO stage I or II disease (81.8% vs 66.7%). Endometriosis was confirmed in 20% of cases before the diagnosis of clear cell carcinoma and 70% of all cases were diagnosed as endometriosis after complete staging surgery. The mean latent period from the diagnosis of endometriosis to that of clear cell carcinoma was 65.6 months. The existence of endometriosis was not related to the prognosis. 결론: Asymptomatic patients with clear cell carcinoma diagnosed during regular gynecologic examination were likely to have smaller tumor lesion size with better oncologic outcomes. More frequent gynecologic check up is needed to detect clear cell carcinoma associated with endometriosis. Early surgical treatment is required for this patient group.

A Proteomic Analysis of Post-translational Modifications in Stress-Induced Signaling Pathway

Lee, Kong-Joo 이화여자대학교 세포신호전달연구센터 2002 고사리 세포신호전달 심포지움 Vol. No.4

Several lines of evidence indicate that protein modifications could be occurred in signaling pathways during cell proliferation, cell death and various stresses. Integrated technologies including protein separation, identification, characterization and information manage system are essential to analyze the modified proteins. This presentation will focus on the analytical strategies for protein modifications using sample preparation using affinity chromatography, 2-dimensional gel electrophoresis, processing of protein spots and identification of proteins using MALDI-TOF MS and tandem MS analysis. In this work, we applied this technology for the identification of modified proteins(phosphorylation and sumoylation) after various stresses, the determinations of protein modification sites. The developed integrated proteome technologies are very useful to understand the biological phenomena at molecular level by identifying the new molecules and their modifications in various cellular processes.

Application of Capillary Electrophoresis on the Structural Studies of Biomolecules

Lee, Kong-Joo 梨花女子大學校 藥學硏究所 1997 藥學硏究論文集 Vol.- No.6

SIGNALING MOLECULES IN STRESS-INDUCED APOPTOSIS

Lee, Kong-Joo 이화여자대학교 세포신호전달연구센터 2000 고사리 세포신호전달 심포지움 Vol. No.2

It is well known that exposure of cells to various environmental stresses such as heat shock and oxidant, hypoxia, heavy metals, growth factors, death surface receptors and various chemicals, can induce apoptotic cell death. However, it is suggested that the apoptotic signaling pathways in response to various stresses are differnt. We investigated the cellular changes by heat shock, ceramide and oxident(H₂O₂ and diamide) in thermotolerant radiation induced fibrosarcoma cells(TR-RIF-1) which have elevated levels of heat shock proteins(hsps). Thermotolerant cells were significantly resistant to apoptosis induced by heat shock and diamide, but no differnt response to ceramide and H₂O₂. To elucidate these differences, the biochemical changes in cells exposed to various stresses were investigated: the extents of apoptosis, protein synthesis, hsp formation, activation of SAPK and ERK activation, no oxidation mediated, on the other hand, membrane disturbing agent ceramide and non-specific oxident H₂O₂ have different pathways as protein denaturants. Simultaneously, the oxidized proteins in cells exposed to oxidants were identified by labeling the active site of glyceraldehyde-3-phosphate dehydrogenase(GAPDH) is a target of oxidants and could be a turn on/off switch of cell death and survival. The cellular signaling mechanism in response to these oxidants were investigated by MALDI-TOF-MS and identified that vimentin, tubulin and 14-3-3 protein are involved in the apoptosis induced by protein denaturation and ROS formation by growth factors. Rac 1 is one of the Rho family members, which acts as a molecular switch to control various cellular processes, including the reorganization of actin cytoskeleton, gene transcription, oncogenicity, cell cycle etc. It is also known that the activation of c-jun N-terminal kinase(JNK) by growth factor is mediated by Rac 1 and phosphoinositide 3-kinase(PI3K), the upstream regulator of rac 1. In this study, the relationship between heat shock responses and rac 1 has been examined. We used rat2, mouse fibroblast cells which permanently overexpress the dominant negative mutant, RacN17. We found that rat2 cells overexpressing RacN17 were tolerant to heat shock and the mechanism of (이후원문누락)