Potato lipoxygenase genes associated with development and pathogen defense response

Kolomiets, Michael V.,Chen, Hao,Braun, E.J.,Gladon, Richard J.,Hannapel, David J. 영남대학교 마늘연구소 2001 의성군 ·영남대학교 관 ·학협동, 협약 조인식 및 심포지움 Vol.2001 No.-

Plant lipoxygenases (LOXs) are a functionally diverse class of dioxygenases implicated in physiological processes such as growth, senescence, and stress-related responses. LOXs incorporate oxygen into their fatty acid substrates and produce hydroperoxide fatty acids that are precursors of jasmonic acid and related compounds. Here we report the involvement of a potato class-1 LOX, POTLX-1, in the control of tuber growth and a unique LOX, POTLX-3, in defense responses against pathogens. RNA hybridization analysis showed that accumulation of Lox1-class transcripts was specific to developing tubers and that mRNA accumulation correlated positively with tuber initiation and growth. Suppression mutants produced by expressing antisense coding sequence of the tuber LOX, POTLX-1, exhibited a significant reduction in LOX activity in stolons and tubers. The suppression of LOX activity correlated with reduced tuber yield, decreased average tuber size, and a disruption of tuber formation. POTLX-3 mRNA accumulation was induced specifically in potato leaves infected with either virulent or avirulent strains of Phytophthora infestans, the causal agent of late blight. During the resistance response, POTLX-3 was induced within 6 h, increased steadily through 24 h, and its mRNA continued to accumulate for a week after inoculation. In contrast, when a plant was susceptible to P. infestans, induction of mRNA accumulation in response to inoculation was inconsistent and delayed. LOX activity assayed during an incompatible interaction in leaves peaked three days earlier than during a compatible interaction. POTLX-3 mRNA accumulation also was induced during hypersensitive response development caused by the incompatible pathogen Pseudomonas syringae pv. phaseolicola. Our results show that LOXs of potato function in diverse roles in both development and in defense responses against pathogen infection.

Anomalous Increase of TC in UGa2 under Pressure

Alexandre Kolomiets,Ladislav Havela,Jiˇr´ı Prchal,Alexander V. Andreev 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.10

UGa2 is 5f ferromagnet with TC = 126 K. High-pressure study of a single crystal exhibits a dramatic increase of TC without any sign of saturation, reaching 155 K at p = 7 GPa, corresponding to +4 K/GPa. Such effect can be understood assuming a weak 5f delocalization and an exchange interaction driven by the hybridization with non-f states, which is strengthened under pressure.

연해주 한까호부근 청동기시대~중세 유적 지표조사 보고

강인욱,양시은,Kolomiets, S.A. 한국고대학회 2005 先史와 古代 Vol.22 No.-

In this report, authors reports the latest site survey on the Khanka lake region. Promorie, Russia. Mainly. the surveys were concentrated on the bronze age and medieval age sites. Methods for investigating sites include collecting surface artefacts, naming GPS data, and gathering carbon for C14 dating. Khanka lake region is located on the borderline with China(Yanbian korean autonomous region) and Tumen valley region of Korean Peninsula, so we hope that our preliminary survey shed light on the study of ancient culture in the Southern part of Primorie, poorly known in spite of increased interests in korean archaeology.

Regulation of Fumonisin Biosynthesis in Fusarium verticillioides-Maize System

Uma Shankar Sagaram,Mike Kolomiets,Won-Bo Shim 한국식물병리학회 2006 Plant Pathology Journal Vol.22 No.3

Fumonisins are a group of mycotoxins produced by a pathogen Fusarium verticillioides in infected maize kernels. Consumption of fumonisin-contaminated maize has been implicated in a number of animal and human illnesses, including esophageal cancer and neural tube defects. Since the initial discovery, chemistry, toxicology, and biology of fumonisins as well as the maize- Fusarium pathosystem have been extensively studied. Furthermore, in the past decade, significant progress has been made in terms of understanding the molecular biology of toxin biosynthetic genes. However, there is a critical gap in our understanding of the regulatory mechanisms involved in fumonisin biosynthesis. Here, we review and discuss our current knowledge about the molecular mechanisms by which fumonisin biosynthesis is regulated in F. verticillioides. In addition, we discuss the impact of maize kernel environment, particularly sugar and lipid molecules, on fumonisin biosynthesis.

Regulation of Fumonisin Biosynthesis in Fusarium verticillioides-Maize System

Sagaram Uma Shankar,Kolomiets Mike,Shim Won-Bo The Korean Society of Plant Pathology 2006 Plant Pathology Journal Vol.22 No.3

Fumonisins are a group of mycotoxins produced by a pathogen Fusarium verticillioides in infected maize kernels. Consumption of fumonisin-contaminated maize has been implicated in a number of animal and human illnesses, including esophageal cancer and neural tube defects. Since the initial discovery, chemistry, toxicology, and biology of fumonisins as well as the maize-Fusarium pathosystem have been extensively studied. Furthermore, in the past decade, significant progress has been made in terms of understanding the molecular biology of toxin biosynthetic genes. However, there is a critical gap in our understanding of the regulatory mechanisms involved in fumonisin biosynthesis. Here, we review and discuss our current knowledge about the molecular mechanisms by which fumonisin biosynthesis is regulated in F. verticillioides. In addition, we discuss the impact of maize kernel environment, particularly sugar and lipid molecules, on fumonisin biosynthesis.

A 57-MHz CW RFQ for the AEBL Project

D.L. Schrage,A. Barcikowski,A.A. Kolomiets,B. Rusthoven,B. Clifft,F. DePaola,G. Waldschmidt,J.W. Rathke,M. Bracken,N.E. Vinogradov,P.N. Ostroumov,S. Sharma,S.I. Sharamentov,T. Schultheiss,W. F. Toter 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.I

The Advanced Exotic Beam Laboratory (AEBL) at the Argonne National Laboratory (ANL) will provide a research facility for studies of nuclear phenomena by using beams of short-lived isotopes for research in the nature of nucleonic matter, the origin of the elements, tests of the Standard Model along with applications in medicine, industry, and other applied physics research. The proposed design of the AEBL driver linac evolved from the Rare Isotope Accelerator (RIA) project. It is a CW 850 MV linac capable of accelerating uranium ions up to 200 MeV/u and protons to 570 MeV with 400 kW beam power. The first section of the linac is a 57 MHz pseudo split coaxial CW Radio Frequency Quadrupole (RFQ) linac. This is followed by 221 superconducting cavities of various types. A section of the RFQ linac was fabricated and tested under R\&D funding for the RIA Project. This is the first section of the six-section, 392 cm RFQ linac. This paper describes the design, fabrication, and testing of this RFQ.

Onset of Magnetic Order in U2(Ni1−xFex)2Sn-H

S. Maˇskov´a,L. Havela,A. Kolomiets,K. Miliyanchuk,A. V. Andreev,H. Nakotte,J. Peterson,Y. Skourski,S. Yasin,S. Zherlitsyn,J. Wosnitza 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.10

We present specific heat and magnetic studies as a function of temperature and magnetic field for U2(Ni1−xFex)2Sn alloys (x = 0.0, 0.05, 0.1, 0.15, 0.2, 0.4, 0.6 and 0.8) and their hydrides (absorption of ≈ 2 H/f.u.). For the parent alloys, antiferromagnetic order is rapidly suppressed with increasing Fe concentration and non-Fermi-liquid behavior was found for x = 0.2. Hydrogenation of the parent alloys causes a substantial increase of TN in Fe-low hydrides (x < 0.2), while Fe-rich hydrides show an unexpected appearance of ferromagnetism in the range 0.4 ≤ x ≤ 0.8. Some of the findings are compared with similar studies in U2Co2Sn and U2Co2InH1.9.

A 12-MHz CW RFQ for the AEBL Project

D. L. Schrage,P. N. Ostroumov,A. Barcikowski,D. Fallin,A. A. Kolomiets 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.3

The Advanced Exotic Beam Laboratory (AEBL) at the Argonne National Laboratory (ANL) will provide a research facility for studies of nuclear phenomena by using beams of short-lived isotopes for research on the nature of nucleonic matter and the origin of the elements, for tests of the Standard Model, for applications in medicine and industry, and for other applied physics research. The proposed design of the AEBL driver linac evolved from the Rare Isotope Accelerator (RIA) project. The AEBL will be a CW linac capable of accelerating uranium ions up to 200 MeV/u and protons to 580 MeV with 400 kW beam power. The AEBL facility also includes a post-accelerator which is designed for acceleration of radioactive ions with charge-to-mass ratios in the range from 1/238 to 1/6. Very low charge-state ions can be most eciently bunched and accelerated by using normally-conducting radio-frequency quadrupole (RFQ) for the rst few MV of the post accelerator. A two-meter long, 12-MHz CW RFQ was designed, built, and tested in the late 1990s as the rst section of a three-section RFQ [18]. This RFQ achieved inter-electrode voltages of 110 kV CW (the peak surface eld was 15 MV/m) and accelerated beams with A/q as large as 132 (132Xe). The AEBL requires a similar RFQ for the post-acceleration of singly-charged unstable nuclides. Our plan is to replace the vanes of this RFQ with a design that incorporates a stronger focusing and that will achieve a higher peak surface eld (16 MV/m) at 82.2-kV inter-vane voltage. The objectives of this project are 1. to conrm the possibility of a low injection energy of 0.4 keV/u which signicantly reduces the voltage required for a high-voltage deck; 2. to test the highest possible peak surface eld on the RFQ electrodes designed for the lowest frequency of 12 MHz compared to existing RFQs worldwide; 3. to provide a technical base for the design of a post-accelerator for the future Advanced Exotic Beam Facility. At the present time, the design is complete, and the fabrication of the 12 MHz RFQ is scheduled to commence in October 2007 with testing planned in 2008. The physics and engineering design of the RFQ is discussed. The Advanced Exotic Beam Laboratory (AEBL) at the Argonne National Laboratory (ANL) will provide a research facility for studies of nuclear phenomena by using beams of short-lived isotopes for research on the nature of nucleonic matter and the origin of the elements, for tests of the Standard Model, for applications in medicine and industry, and for other applied physics research. The proposed design of the AEBL driver linac evolved from the Rare Isotope Accelerator (RIA) project. The AEBL will be a CW linac capable of accelerating uranium ions up to 200 MeV/u and protons to 580 MeV with 400 kW beam power. The AEBL facility also includes a post-accelerator which is designed for acceleration of radioactive ions with charge-to-mass ratios in the range from 1/238 to 1/6. Very low charge-state ions can be most eciently bunched and accelerated by using normally-conducting radio-frequency quadrupole (RFQ) for the rst few MV of the post accelerator. A two-meter long, 12-MHz CW RFQ was designed, built, and tested in the late 1990s as the rst section of a three-section RFQ [18]. This RFQ achieved inter-electrode voltages of 110 kV CW (the peak surface eld was 15 MV/m) and accelerated beams with A/q as large as 132 (132Xe). The AEBL requires a similar RFQ for the post-acceleration of singly-charged unstable nuclides. Our plan is to replace the vanes of this RFQ with a design that incorporates a stronger focusing and that will achieve a higher peak surface eld (16 MV/m) at 82.2-kV inter-vane voltage. The objectives of this project are 1. to conrm the possibility of a low injection energy of 0.4 keV/u which signicantly reduces the voltage required for a high-voltage deck; 2. to test the highest possible peak surface eld on the RFQ electrodes designed for the lowest frequency of 12 MHz compared to existing RFQs worldwide; 3. to provide a technical base for the design of a post-accelerator for the future Advanced Exotic Beam Facility. At the present time, the design is complete, and the fabrication of the 12 MHz RFQ is scheduled to commence in October 2007 with testing planned in 2008. The physics and engineering design of the RFQ is discussed.

동북아시아 고고학의 최근 연구 성과 ; 연해주 한까호부근 청동기시대~중세 유적 지표조사 보고

강인욱 ( In Uk Kang ),양시은 ( Si Eun Yang ),( S. A. Kolomiets ) 한국고대학회 2005 先史와 古代 Vol.22 No.-

9,10-KODA, an α-ketol Produced by the Tonoplast-localized 9-lipoxygenase ZmLOX5, Plays a Signaling Role in Maize Defense against Insect Herbivory

Yong-Soon Park,Yuhyeon Song,Hyo Gil Choi,Michael V. Kolomiets 한국원예학회 2023 한국원예학회 학술발표요지 Vol.2023 No.10