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Search for sub-GeV dark matter by annual modulation using XMASS-I detector
Kobayashi, M.,Abe, K.,Hiraide, K.,Ichimura, K.,Kishimoto, Y.,Kobayashi, K.,Moriyama, S.,Nakahata, M.,Ogawa, H.,Sato, K.,Sekiya, H.,Suzuki, T.,Takeda, A.,Tasaka, S.,Yamashita, M.,Yang, B.S.,Kim, N.Y.,K North-Holland Pub. Co 2019 Physics letters. Section B Vol.795 No.-
<P><B>Abstract</B></P> <P>A search for dark matter (DM) with mass in the sub-GeV region (0.32–1 GeV) was conducted by looking for an annual modulation signal in XMASS, a single-phase liquid xenon detector. Inelastic nuclear scattering accompanied by bremsstrahlung emission was used to search down to an electron equivalent energy of 1 keV. The data used had a live time of 2.8 years (3.5 years in calendar time), resulting in a total exposure of 2.38 ton-years. No significant modulation signal was observed and 90% confidence level upper limits of 1.6 × <SUP> 10 − 33 </SUP> cm<SUP>2</SUP> at 0.5 GeV was set for the DM-nucleon cross section. This is the first experimental result of a search for DM mediated by the bremsstrahlung effect. In addition, a search for DM with mass in the multi-GeV region (4–20 GeV) was conducted with a lower energy threshold than previous analysis of XMASS. Elastic nuclear scattering was used to search down to a nuclear recoil equivalent energy of 2.3 keV, and upper limits of 2.9 × <SUP> 10 − 42 </SUP> cm<SUP>2</SUP> at 8 GeV was obtained.</P>
EFFECTS OF FRUCTO-OLIGOSACCHARIDES ON MILK-YIELD AND MILK-COMPONENTS OF DAIRY COWS
Kobayashi, S.,Eida, T. Asian Australasian Association of Animal Productio 1990 Animal Bioscience Vol.3 No.1
Fructo-oligosaccharides are found in many plants, such as onion, burdock and wheat. They are not well hydrolyzed by digestive enzymes in animals, but are peculiarly assimilated by Bifidobacterium and some useful bacteria. In our previous experiment (Kobayashi et al., 1987) it was suggested that they were effective in decreasing energy loss in the metabolism of dairy calves. In the present study, the effects of fructo-oligosaccharides on body weight, milk-yield and milk-components (fat, protein and solids-not-fat) were investigated in dairy cows. Lactating cows were fed a standard diet containing fructoligo saccharides at 18.70g, 9.35g and 0.0g (control) per 100kg body weight, day for three weeks. Neither treatments significantly affected any of the parameters examined. The fructo-oligosaccharides were assumed to be hydrolyzed by rumen microorganisms and hardly to affect the bacterium florae in the intestines of the lactating cows.
Examining the Effects of Metacognitive Instruction in Oral Communication for EFL Learners
Ayako Kobayashi 아시아영어교육학회 2020 The Journal of Asia TEFL Vol.17 No.2
This study investigated the effects of metacognitive instruction in oral communication for EFL learners over a semester. Participants (N = 58) were sampled from four oral communication classes in a private university in the western part of Japan where the researcher had been working as an English instructor. They were freshmen whose majors were not English. The treatment group (n = 28) received metacognitive instruction in oral communication (i.e., they were taught how to improve oral communication skills and how to become more autonomous learners through metacognitive processes as well as activities such as planning, monitoring, and reflecting). The contrast group (n = 30), taught by the same teacher, did not receive metacognitive instruction although they used the same textbooks and materials and had more time for interaction in English. Results of the questionnaire (SRLQ, the Self-Regulated Learning in Oral Communication Questionnaire, Kobayashi, 2016a) showed that students in the treatment group became more self-regulated learners. Moreover, it was found that the treatment group made greater gains in interactional competence, regulation of cognition, cognitive strategies and interaction strategies. Furthermore, it was verified that learners with low interactional competence in the treatment group benefited the most from such metacognitive instruction.
Overview of Arabidopsis Resource Project in Japan
Kobayashi, Masatomo Korean Society for Bioinformatics 2011 Interdisciplinary Bio Central (IBC) Vol.3 No.1
Arabidopsis is well-known to the world's plant research community as a model plant. Many significant resources and innovative research tools, as well as large bodies of genomic information, have been created and shared by the research community, partly explaining why so many researchers use this small plant for their research. The genome sequence of Arabidopsis was fully characterized by the end of the $20^{th}$ century. Soon afterwards, the Arabidopsis research community began a 10-year international project on the functional genomics of the species. In 2001, at the beginning of the project, the RIKEN BioResource Center (BRC) started its Arabidopsis resource project. The following year, the National BioResource Project was launched, funded by the Japanese government, and the RIKEN BRC was chosen as a core facility for Arabidopsis resource. Seeds of RIKEN Arabidopsis transposon-tagged mutant lines, activation-tagged lines, full-length cDNA over-expresser lines, and natural accessions, as well as RIKEN Arabidopsis full-length cDNA clones and T87 cells, are preserved at RIKEN BRC and distributed around the world. The major resources provided to the research community have been full-length cDNA clones and insertion mutants that are suitable for use in reverse-genetics studies. This paper provides an overview of the Arabidopsis resources made available by RIKEN BRC and examples of research that has been done by users and developers of these resources.
Application of Molecular Biology to Rumen Microbes -Review-
Kobayashi, Y.,Onodera, R. Asian Australasian Association of Animal Productio 1999 Animal Bioscience Vol.12 No.1
Molecular biological techniques that recently developed, have made it possible to realize some of new attempts in the research field of rumen microbiology. Those are 1) cloning of genes from rumen microorganisms mainly in E. coli, 2) transformation of rumen bacteria and 3) ecological analysis with nonculturing methods. Most of the cloned genes are for polysaccharidase enzymes such as endoglucanase, xylanase, amylase, chitinase and others, and the cloning rendered gene structural analyses by sequencing and also characterization of the translated products through easier purification. Electrotransformation of Butyrivibrio fibrisolvens and Prevotella ruminicola have been made toward the direction for obtaining more fibrolytic, acid-tolerant, depoisoning or essential amino acids-producing rumen bacterium. These primarily required stable and efficient gene transfer systems. Some vectors, constructed from native plasmids of rumen bacteria, are now available for successful gene introduction and expression in those rumen bacterial species. Probing and PCR-based methodologies have also been developed for detecting specific bacterial species and even strains. These are much due to accumulation of rRNA gene sequences of rumen microbes in databases. Although optimized analytical conditions are essential to reliable and reproducible estimation of the targeted microbes, the methods permit long term storage of frozen samples, providing us ease in analytical work as compared with a traditional method based on culturing. Moreover, the methods seem to be promissing for obtaining taxonomic and evolutionary information on all the rumen microbes, whether they are culturable or not.
Year-round Monitoring of Verotoxin-producing Escherichia coli from Feces of Dairy Cattle
Kobayashi, Y.,El-Sawy, H.B.I. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5
A PCR-aided monitoring of verotoxin-producing Escherichia coli (VTEC) was performed over the period of 12 months by using fresh feces collected monthly from 5 dairy cows that had been identified as VTEC carriers. The PCR products were confirmed to be verotoxin genes by Southern hybridization using a gene fragment of verotoxin 2 as a probe. Although seasonal variation of VTEC shedding seemed to depend on each cow, several factors may have influenced the frequency of detection. Shedding of VTEC tended to be reduced during grazing from the middle of May up to the beginning of October. Only one cow was positive for VTEC in August. Dry-off was also suggested to have a depressive effect on VTEC shedding, i.e. 3 of 4 dry cows showed no shedding of VTEC. Contrary to these factors, winter or indoor rearing tended to increase VTEC with only 5/24 samples being negative during the period from November to April. Total VFA concentration was higher (p<0.05) in VTEC-positive feces than in VTEC-negative feces, while fecal pH and VFA proportions were not different. Partial sequences of verotoxin genes from feces of 4 VTEC-positive cows were nearly identical (99-100%), suggesting that gut bacteria sharing the same gene were distributed among the cows. The present results indicate that grazing and dry-off could be factors which reduce VTEC shedding, while winter/indoor rearing may be a factor which increases the shedding, possibly through on-farm interactions.