Fumigant activity of phosphine against wood boring insects

Kwang-Soon Choi,Jun-Won, Park,Hyun Kyung Kim,Gil-Hah Kim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

Wood boring insects are the major problem of many industries, such as hardwood brokers, importers, merchants, and manufacturers of furniture. Phosphine (PH3) is highly toxic to insects and is known to penetrate treated material. In this study, effectiveness of PH3 which is altered methyl bromide, was examined with Monochamus saltuaris, Platypus koryoensis, Cryphalus fulvus, and Xyleborus mutilatus. Fumigation to M. saltuaris, P. koryoensis, C. fulvus, and X. mutilatus was carried in a desiccator (12L) at 20℃ for 24 h. As a result, LC99 of PH3 to M. saltuaris, P. koryoensis, C. fulvus, and X. mutilatus was showed 0.455 mg L-1, 0.804 mg L-1, 0.243 mg L-1 and 0.046 mg L-1 in a desiccator(12L) at 20℃, respectively. M. saltuaris, P. koryoensis, C. fulvus, and X. mutilatus 100 % mortality was showed in a fumigation chamber (10 m3 and 100 m3) at normal temperature. In these results indicates that PH3 described could be useful for fumigant agent of 4 species wood pests.

Application of CRAMPS for a Phase Transition in H⁺-ion irradiated TlH₂PO₄

Kim, Se-Hun,Han, J.H.,Lee, Cheol-Eui,Lee, Kwang-Sei,Kim, Chang-Sam,Dalal, N.S.,Han, Doug-Young Korean Magnetic Resonance Society 2010 Journal of the Korean Magnetic Resonance Society Vol.14 No.2

We studied the hydrogen-bonded $TlH_2PO_4$ (TDP) ferroelectrics treated with the proton-beam bombardment. The TDP material was irradiated with 1-MeV proton beam at a dose of $10^{15}/cm^2$. In order to analyze the hydrogen environment in TDP, we carried out the $^1H$ high resolution nuclear magnetic resonance (NMR) - i.e., Combined Rotation And Multiple Pulse Spectroscopy (CRAMPS) measurement. The isotropic chemical shift of hydrogen indicates its displacive property is related to the $PO_4$ lattice deformation which occurs throughout the antiferroelectric-, the ferroelastic- and the paraelastic-phase transitions. The temperature dependence of $\sigma_{iso}$ reveals the electronic charge redistribution is induced by the proton-beam irradiation and the elastic property.

촉매 지지체의 기공특성에 대한 가교제 및 용매의 효과

김광락,정흥석,강희석,이한수,안도희,백승우,이성호 한국공업화학회 1998 응용화학 Vol.2 No.2

Effect of cross-linking agent and solvent on pore characteristics of styrene-divinylbenzene copolymer was investigated experimentally. Influence of cross-linking agent on surface area of the polymeric support was dominant over all the others. Surface area and pore volume of the polymeric support were decreased and the second peak was shifted to large pore size in the pore size distribution with increasing the content of poor solvent in the solvent mixture. When toluene was used as a good solvent, and n-heptane as poor solvent, the optimum range of good/poor solvent ratio was 1.5∼0.7 for styrene-divinylbenzene copolymer of large surface and micropore area and pore volume.

Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity

Bayne, Elizabeth H.,White, Sharon A.,Kagansky, Alexander,Bijos, Dominika A.,Sanchez-Pulido, Luis,Hoe, Kwang-Lae,Kim, Dong-Uk,Park, Han-Oh,Ponting, Chris P.,Rappsilber, Juri,Allshire, Robin C. Cell Press 2010 Cell Vol.140 No.5

<P><B>Summary</B></P><P>In fission yeast, RNAi directs heterochromatin formation at centromeres, telomeres, and the mating type locus. Noncoding RNAs transcribed from repeat elements generate siRNAs that are incorporated into the Argonaute-containing RITS complex and direct it to nascent homologous transcripts. This leads to recruitment of the CLRC complex, including the histone methyltransferase Clr4, promoting H3K9 methylation and heterochromatin formation. A key question is what mediates the recruitment of Clr4/CLRC to transcript-bound RITS. We have identified a LIM domain protein, Stc1, that is required for centromeric heterochromatin integrity. Our analyses show that Stc1 is specifically required to establish H3K9 methylation via RNAi, and interacts both with the RNAi effector Ago1, and with the chromatin-modifying CLRC complex. Moreover, tethering Stc1 to a euchromatic locus is sufficient to induce silencing and heterochromatin formation independently of RNAi. We conclude that Stc1 associates with RITS on centromeric transcripts and recruits CLRC, thereby coupling RNAi to chromatin modification.</P>

Expression of the Blue Fluorescent Protein (AmCyan) in the Fibroin of Transgenic Silkworms

Seon Young Kim,Seong Wan Kim,Eun Young Yun,Kwang-Ho Choi,Seong Ryul Kim,Seok Woo Kang,Seung Won Park,Tae Won Goo 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

To product the blue fluorescent protein (AmCyan) expressed cocoon, we were fused AmCyan cDNA to the heavy chain gene and injected the gene into a silkworm. AmCyan was one of the existing violet fluorochromes and originally derived from the fluorescent protein amFP486. AmFP486 was cloned from the sea anemone Anemonia majano (GenBank accession number AF168421), and belongs to the family of fluorescent proteins (FPs) isolated from coral reef organisms. The AmCyan fusion protein, each with N- and C- terminal sequences or the fibroin H-chain, were designed to be secreted into the lumen of the posterior silk glands. The expression of the AmCyan/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the 3xP3 EGFP as a marker allowed us to rapidly distinguish transgenic silkworm. Mixtures of the donor and helper vectors were micro-injected into 300 eggs of bivoltine silkworms (Baegokjam). EGFP fluorescence was observed in 3 broods of transgenic silkworms under a florescence stereomicroscope. The cocoon was displayed strong blue fluorescence, proving that the fusion protein was present in the cocoon. Accordingly, we suggest that the AmCyan gene expressed cocoon will be enable the production of the novel biomaterials based on the transgenic silk.

국내 TMR의 입자도에 관한 조사 연구

기광석,김현섭,정하연,이현준,안병석,김준식,강수원,김용국,하종규 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.5

This study was carried out to survey operation system of self-making TMR and particle size of self-making and commercial TMR, then to compare TMR particle size recommended by Penn State particle Size Separator. Daily mixing time in self-making TMR averaged 48.6±28.0min, 2 angar type of mixer was most by 55.5% and daily 2 times of mixing in summer and other season was 44.4 and 22.2%, respectively. Percentage of residual feed in upper sieve(19㎜ diameter), middle sieve(8 ti 18㎜ diameter) and lower pen was 21.5~25.6%, 16.0~25.8%, 52.8~61.4%, respectively, at self-making TMR mixer. Percentage of particle sizes of more than 19㎜ in self-making and commercial TMR was 24.9±1.4 and 26.2±1.7%, that of 8-10㎜ 22.8±1.0 and 12.8±1.2 and that of less than 8㎜ 52.3±1.7 and 61.0±1.5, respectively, which in particle length of 8~13㎜ in self-making and commercial TMR was lower by 82.6 and 100% compared to that recommended by Penn State particle separator, respectively. Especially there was not particle length of more than 19㎜ in commercial TMR at all. It is necessary to check the number of cows ruminating in a farm in order to estimate the particle size of TMR; it is recommended to change the TMR mixing time or the TMR formulae if the proportion of ruminating cows in a farm is less than 40%.

Microstructure and Mechanical Properties of Twin-Roll Strip Cast Mg Alloys

Park, Sung S.,Bae, Geun Tae,Lee, Jung G.,Kang, Dae H.,Shin, Kwang Seon,Kim, Nack J. Scientific.Net 2007 Materials Science Forum Vol.539-543 No.1

<P>Development of wrought Mg alloys, particularly in sheet form, is essential to support the growing interest for lightweight components in the automotive industry. However, development of Mg alloy sheets has been quite slow due to the complexity of sheet production originated from limited deformability of Mg. In this respect, twin-roll strip casting, a one-step processing of flat rolled products, can be an alternative for the production of Mg alloy sheets. In this study, AZ31 and experimental ZM series alloys are twin-roll strip cast into 2 mm thick sheets. The microstructure of the as-cast AZ31 alloy sheet consists of columnar zones near the roll side and equiaxed zones in the mid-thickness region. On the other hand, as-cast ZM series alloy sheets show equiaxed dendritic structure through the thickness of sheet. These alloys were subjected to various thermo-mechanical treatments and their tensile properties were evaluated. Twin-roll strip cast AZ31 alloy in H24 condition has equivalent yield and tensile strengths with similar ductility compared to commercial ingot cast AZ31-H24 alloy, indicating that twin-roll strip casting is a viable process for the fabrication of Mg alloy sheets. The experimental ZM series alloys have a large volume fraction of fine dispersoid particles in the microstructure, resulting from the beneficial effect of twin-roll strip casting on microstructural refinement. It has been shown that the experimental ZM series alloys have superior tensile properties compared to commercial ingot cast AZ31-H24 alloy, suggesting the possibility of the development of new wrought Mg alloy sheets by twin-roll strip casting.</P>

기관기관지 골형성증 2예 : 분절기관지 침범 1예 포함 Including one case which showed involvement of the segmental bronchi

김윤정,구성현,이선민,박광주,황성철,이이형,한명호,주희재,이기범,박찬희 아주대학교 의과학연구소 1999 아주의학 Vol.4 No.1

저자들은 기침, 흉통 등을 주소로 내원한 환자와 우연히 흉부 방사선 검사에서 이상이 발견된 환자에서 기관지경 생검으로 기관기관지 골형성증을 확진한 2예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Tracheobronchopathia osteochondroplatica (TPO) is a rare disease characterized by the presence of multiple ossified or cartilaginous sessile nodules mainly within the submucosa of the trachea and bronchi. The patients are usually asymptomatic. Sometimes, however, cough, hemoptysis, dyspnea, or recurrent respiratory infection may develop. Its etiology is not known, but is considered" to be benign. In the past, most of the cases were diagnosed incidentally at autopsy. But with an introduction of bronchoscopy and computed tomography, antemortem diagnosis has been reported. We report herein two cases of TPO (including a case which showed involvement of the segmental bronchi) diagnosed by computed tomography, ^(99m)Technetium-MDP thorax scan, bronchoscopic examination and biopsy. A brief review of the literature is also given.

U-서울 도래와 서울시 교통대응 전략

이광훈(Kwang-Hoon Lee),박호진(H0-Jin Park),김희진(Hee-Jin Kim) 서울연구원 2006 서울연구원 정책과제연구보고서 Vol.- No.-

S. pneumoniae 호흡기감염 마우스에서 Ciprofloxacin의 항균력에 대한 조직학적 연구

구세광,이형식,김종대,최해윤,이재현 慶山大學校 基礎科學硏究所 2002 基礎科學 Vol.6 No.1

Sterptococcus pneumoniae ATCC 6303을 이용한 국소 호흡기 감염 마우스 모델에서 Ciprofloxacin (CPFX)의 in vivo 항균력을 조직학적으로 평가하기 위하여 생균수와 폐의 조직·병리학적 변화를 관찰하였던 결과, 다음과 같은 결과를 얻었다. 1) 생균수는 CPFX 투여군에서 Control 군에 비해 유의성 있게 감소되었으며, 2) Control 군에서는 현저한 폐내 염증세포의 침윤, 출혈 및 폐포 벽의 비후가 조직학적으로 관찰되었으나, 이러한 조직학적 소견들은 CPFX 투여군에서 Control 군에 비해 현저히 있게 감소되었고, 3) LSA (luminal surface of alveoli %)는 Control 군에서 Sham 군에 비해 현저히 감소된 반면, CPFX 투여군에서 Control 군에 비해 유의성 있게 증가되었다. 이상에서 본 실험의 결과, CPFX의 Sterptococcus pneumoniae에 대한 항균력이 조직학적으로 관찰되었다. In order to evaluate the in vivo effect of ciprofloxacin (CPFX), the viable bacterial number and histopathological changes were monitored after experimental respiratory infection with Sterptococcus pneumoniae ATCC 6303. In CPFX, the viable bacterial numbers were significantly and dose-dependently decreased compared to that of Control group. In Control group, severe infiltration of inflammatory cells, hemorrhage and hypertrophy of alveolar linings were demonstrated at microscopical levels. However, these abnormal histopathological changes were significantly and dose-dependently decreased compared to that of Control group in CPFX group. And also in CPFX group, the LSA (luminal surface of alveoli %) were significantly and dose-dependently increased compared to that of Control group. According to these results, it is considered as the in vivo antibacterial activity of CPFX was histologically showed against Sterptococcus pneumoniae ATCC 6303 infection of respiratory tract in this study.