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Nanomechanical actuation driven by light-induced DNA fuel
Eom, Kilho,Jung, Huihun,Lee, Gyudo,Park, Jinsung,Nam, Kihwan,Lee, Sang Woo,Yoon, Dae Sung,Yang, Jaemoon,Kwon, Taeyun The Royal Society of Chemistry 2012 Chemical communications Vol.48 No.7
<P>We report the reversible nanomechanical actuation of a microcantilever driven by the light irradiation-induced conformational changes of i-motif DNA chains, which are functionalized on the cantilever's surface. It is shown that light irradiation-driven nanomechanical actuation can be manipulated using DNA hybridization and/or ionic concentrations.</P> <P>Graphic Abstract</P><P>We have first demonstrated the nanomechanical actuations using light irradiation-driven DNA fuel. Our study shows that cantilever bending motion can be reversibly controlled by light-driven DNA fuel. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1cc12893k'> </P>
Nam, Kihwan,Eom, Kilho,Yang, Jaemoon,Park, Jinsung,Lee, Gyudo,Jang, Kuewhan,Lee, Hyungbeen,Lee, Sang Woo,Yoon, Dae Sung,Lee, Chang Young,Kwon, Taeyun The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.44
<P>We have developed a horizontally aligned carbon nanotube sensor that enables not only the specific detection of biomolecules with ultra-sensitivity, but also the quantitative characterization of binding affinity between biomolecules and/or interaction between a carbon nanotube and a biomolecule, for future applications in early diagnostics. In particular, we have fabricated horizontally aligned carbon nanotubes, which were functionalized with specific aptamers that are able to specifically bind to biomolecules (<I>i.e.</I> thrombin). Our detection system is based on scanning probe microscopy (SPM) imaging for horizontally aligned aptamer-conjugated carbon nanotubes (ACNTs) that specifically react with target biomolecules at an ultra-low concentration. It is shown that the binding affinity between thrombin molecule and ACNT can be quantitatively characterized using SPM imaging. It is also found that the smart carbon nanotube sensor coupled with SPM imaging permits us to achieve the high detection sensitivity even up to ∼1 pM, which is much higher than that of other bioassay methods. Moreover, we have shown that our method enables a quantitative study on small molecule-mediated inhibition of specific biomolecular interactions. In addition, we have shown that our ACNT-based system allows for the quantitative study of the effect of chemical environment (<I>e.g.</I> pH and ion concentration) on the binding affinity. Our study sheds light on carbon nanotube sensor coupled with SPM imaging, which opens a new avenue to early diagnostics and drug screening with high sensitivity.</P> <P>Graphic Abstract</P><P>ACNT, constructed through the chemical conjugation of aptamer to individual nanotube on the silicon substrate, serves as a nanoscale pattern (without lithography) leading to biomolecular recognition with high sensitivity. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm33688j'> </P>
Lee, Gyudo,Lee, Hyungbeen,Nam, Kihwan,Han, Jae-Hee,Yang, Jaemoon,Lee, Sang Woo,Yoon, Dae Sung,Eom, Kilho,Kwon, Taeyun Springer 2012 Nanoscale research letters Vol.7 No.1
<P>We report on how to quantify the binding affinity between a nanoparticle and chemical functional group using various experimental methods such as cantilever assay, PeakForce quantitative nanomechanical property mapping, and lateral force microscopy. For the immobilization of Au nanoparticles (AuNPs) onto a microscale silicon substrate, we have considered two different chemical functional molecules of amine and catecholamine (here, dopamine was used). It is found that catecholamine-modified surface is more effective for the functionalization of AuNPs onto the surface than the amine-modified surface, which has been shown from our various experiments. The dimensionless parameter (i.e., ratio of binding affinity) introduced in this work from such experiments is useful in quantitatively depicting such binding affinity, indicating that the binding affinity and stability between AuNPs and catecholamine is approximately 1.5 times stronger than that between amine and AuNPs. Our study sheds light on the experiment-based quantitative characterization of the binding affinity between nanomaterial and chemical groups, which will eventually provide an insight into how to effectively design the functional material using chemical groups.</P>