수종의 피부종양에서 Proliferating Cell Nuclear Antigen과 Ki - 67 의 표현에 관한 비교 연구

황선욱(Sun Wook Hwang),원영호(Young Ho Won),전인기(Inn Ki Chun),박창수(Chang Soo Park) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.3

Background : Both PCNA and Ki-67 have been used as marker for cellular proliferation. The drawback of Ki-67 antibody in immunohistochemistry was that it can be labelled only on fresh tissue, however, MIB1 is a newly developed Ki-67 antiboc which can be labelled on formalin-fixed tissue. Objective : The purpase of the present study is to compir the stainability of the Ki-67 antibody with that of the ICNA antibody on formalin-fixed, para fin embedded tissues. Methods : Using MIE1, the newly developed Ki-67 antibody and PC10(PCNA antibody), speci mens of squamous cell carcinoma(SCC), Bowens disease(BL), actinic keratosis(AK) and basal cell epithelioma(BCE) were stained by one hour immunocytial, mistry using a Microprobe immuno/DNA stainer. Results : The labelling indices (LI) of MIB1 were 82.6%, 37.4%, 38.3% & 81.1% respectively in SCC, BD, AK & BC, while the LI of PC10 were 77.69%, 26.6% & 64.4%. The labelled cells of both antibodies differed in distribution patterns on turmor tissues. Conclusion : MIBI cain be used to be an alternative m.rl r for proliferating cells. MIBI PC10, when used together, will be mutually compensatory the study of proliferating cell kinetics. (Kor J Dermatol 1995;33(3): 453-458)

동창의 임상 및 병리조직학적 소견과 Ki-67 발현 양상

서기석(Ki Seuk Suh),김상태(Sang Tae Kim),이기욱(Ki Wook Lee) 대한피부과학회 1999 대한피부과학회지 Vol.37 No.9

N/A Background : Pernio or chilblain is an abnormal reaction to damp cold air, resulting in pruritic erythematous swellings on acral skin. The histologic features are subepidermal edema, diffuse fluffy edema of endothelial cell, and superficial or superficial and deep perivascular lymphocytic infiltrates. Many studies with a large sample size have been conducted in western countries, but there were no collective studies of pernio that have been reported in Korea. Objective : The purpose of this study was to clarify the clinical and histopathologic features of pernio in Korea. Methods : We reviewed the clinical and histopathologic findings in ten patients with pernio who visited the Department of Dermatology, Kosin Medical College Gaspel Hospital during the 10 year period from 1988 to 1997. Results : The results were as follows. 1. The age distribution was in the range of 6∼50 year old. The prevalance was heighest in the third and fourth decade. Sex ratio was 1 : 4 (2 males : 8 females). The duration of symptoms ranged from 4 days to 30 years. All patients developed skin lesions on the acral area. Two male patients developed skin lesions on the hand associated with cold exposure and outdoor works. All patient had itching and burning papules and/or plaques, and one had a painful hard nodule. Laboratory studies were all negative and within normal ranges. 2. Histopathologic features revealed subepidermal edema, diffuse fluffy edema of endothelial cell, and superficial or superficial and deep perivascular lymphocytic infiltrates in all specimens. 3. Treatment includes prophylatic measures against cold and nifedipine(calcium channel inhibiting drug). In more severe cases, pentoxifylline might be helpful. Conclusion : Our study suggests that pernio commonly occurs in twenty to forty year old females during fall and winter. Upon exposure to cold temperatures, the acral skin develops pruritic erythematous papules and plaques. If these lesions persisit for a long time or recur, they may progress to vesicle and ulceration and possibly develop vasculitis which does not respond to therapy well. Thus, a thorough understanding of clinical and histopathologic features of pernio is vital in making a diagnosis, a treatment plan and determining the prognosis. (Korean J Dermatol 1999;37(9) : 1254∼1260)

소화불량(消化不良)과 과심상(過心傷)의 상관성(相關性)에 대(對)한 고찰(考察) -스트레스, 기울(氣鬱), 비병증(脾病證)의 평가(評價)를 통(通)해-

김진성,윤상협,류봉하,류기원,이상욱,Kim, Jin-Sung,Yoon, Sang-Hyub,Ryu, Bong-Ha,Ryu, Ki-Won,Lee, Sang-Wook 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.4

Background & Object : Dyspepsia for which no organic causes are disclosed is referred to as functional dyspepsia. Functional dyspepsia is here studied in connection with a biopsychosocial model. From the aspect of individual response to external environment, in connection with stress response, functional dyspepsia is studied by both the psychology department and the internal medicine departments. The disease is taken as approachable from the aspect of internal injury due to seven emotions and stress as differentiated by Oriental medicine. Materials and Methods : Targeted at 223 patients underwent medical checks and endoscopy at Kangnam Korean Hospital, Kyunghee University. They agreed to join this clinical experiment. Stress response inventory, GARS (global assessment of recent stress scale), GSRS (gastrointestinal symptom rating scale), diagnostic scores for Ki-depression, and Spleen Disease Differentiation of Syndromes were all measured and evaluated. The test group was comprised of functional dyspepsia patients. The control group was comprised of nonsymptomatic chronic gastritis patients who were found to suffer from chronical gastritis in endoscopy and thus could be diagnosed with functional dyspepsia if symptoms would arise, but did not complain of subjective symptoms. Results showed these corelations: Functional dyspepsia patients were found to have more serious Ki-depression compared to nonsymptomatic chronic gastritis patients. The more serious Ki-depression the more serious the dyspepsia symptoms. The higher the stress response inventory the more serious the dyspepsia. Deficiency of spleen Eum, and Deficiency and Sinking of spleen Gi were found to coincide with serious Ki-depression.

Therapeutic Effects of Recombinant Human Epidermal Growth Factor (rhEGF) in a Murine Model of Concurrent Chemo- and Radiotherapy-Induced Oral Mucositis

RYU, Seung-Hee,KANG, Ki Mun,MOON, Soo Young,CHAI, Gyu Young,HONG, Joon Pio,CHO, Kyoung-Oh,KANG, Mun-Il,CHOI, Eun Kyung,LEE, Sang-wook Journal of Radiation Research Editorial Committee 2010 JOURNAL OF RADIATION RESEARCH Vol.51 No.5

<P>Concurrent chemotherapy with radiotherapy (CCRT) has been applied for the treatment of advanced stage of head and neck cancer patients. However CCRT is associated with several complications including mucositis, dermatitis, stomatitis, etc. This study was conducted to evaluate the therapeutic effect of systemically administrated recombinant human epidermal growth factor (rhEGF) in CCRT-induced oral mucositis in a mouse model. Oral mucositis was induced in male BALB/c mice through combination treatment with cisplatin (11 mg/kg, i.p.) and irradiation (17 Gy) of the head and neck area. rhEGF (1.0 mg/kg/day for consecutive 3 days) was administered systemically, and the therapeutic effect was determined by histological evaluation of the oral mucosa. To elucidate optimal dose of rhEGF on CCRT-induced mucositis, various concentrations (0.04–3 mg/kg) of rhEGF were injected for 3 days. Systemic rhEGF administration accelerated the recovery of body weight. Histologically, rhEGF-treated mice showed significantly increased epithelial cell layer thickness, basal cell number, and expression of Ki-67 compared to control mice. Most effective dose was 1 mg/kg among other doses tested. Systemic administration of 1 mg/kg of rhEGF reduces the severity of oral mucositis induced by CCRT in a mouse model, suggesting that rhEGF can be used for treating CCRT-induced mucositis during the cancer treatment.</P>

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

대퇴 전자간 골절의 내고정시 발생한 천부 대퇴 동맥의 관통 손상 : 증례보고

김기수,류상욱,서유석,임동윤 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.1

The superficial femoral arterial injury due to the internal fixation surgery in intertrochanteric femoral fracture has been reported to be an unusual complication case. Recently, we experienced a case of patient with penetrating injury of superficial femoral artery induced by the internal fixation surgery with a gamma nail in an intertrochanteric femoral fracture. This penetrating injury was successfully treated by the transplantation surgery with an artificial blood vessel, which has been not yet reported. Therefore, we report the present clinical result obtained from the case work with reviewing literature, It seems likely that this case is the first report related to penetrating injury of superficial femoral artery induced by the internal fixation surgery with gamma nail.

1,3-Disubstituted Pyrido [2,3-d] Pyrimidinedione

류정욱,이기창,강삼룡 明知大學校 産業技術硏究所 1984 産業技術硏究所論文集 Vol.3 No.-

1,3-Disubstituted pyrido [2, 3-d] pyrimidine-2, 4-diones derivatives were synthesized by the following method A and B. Method A: 1-Substituted pyrido [2, 3,-d] pyrimidine-2, 4-diones were prepared by the reaction of 2-[N-substituted amino] nicotinic acid with urea at high temperature. On the other hand method B:1-Substituted pyrido [2, 3-d] pyrimidine-2 4-dione prepared by the reaction of 2-[N-substituted amino] nicotinamide with ethyl carbonate at low temperature. It was largely affected by catalyst and temperature that the synthesis of 2-[N-substituted amino] nicotinic acid as starting material, and amide of 2-[N-substituted amino] nicotinic and derivative was synthesized by the typical nucleophilic substitution of acyl halide. Considering the reaction time and temperature, however, it is clear that the method B is more efficient than the method A. The former requires only 2-3 hours of reaction time at 20-40℃, while the latter requires 7 hours at 180-200℃. Yields for two methods obtained were 60% all. Yields of 1, 3-disubstituted pyrido [2, 3-d] pyrimidine-2, 4-dione as object was obtained about 60% by the reaction of intermediate material with alkylated in the DMF solvent. It has been affirmed that the mechanism of reactions involved in synthesis was of nucleophilic substitution. The molecular structure, and molecular weight of 1, 3-disubstituted pyrido [2, 3-d] pyrimidine-2, 4-diones were confirmed by the analysis of physicochemical instrumentation such as IR, NMR, and Ms spectra, and the physical properties were examined.

수학교육의 한가지 대안으로서의 Freudenthal 이론

김기민,김종욱 창원대학교 학생생활연구소 2001 학생생활연구 Vol.14 No.-

일본 산촌지역의 고령화와 지역사회 보건ㆍ의료ㆍ복지서비스 체계구축 양상 : 大分縣 湯布阮町의 노인복지기관의 서비스 내용과 기관간의 관계성을 중심으로

엄기욱 전남대학교 아시아태평양지역연구소 2001 아시아태평양지역연구 Vol.3 No.2

본 연구는 고령화와 과소화의 문제를 함께 지니고 있으면서 도시지역과는 다르게 전통적인 지역내부의 권력구조와 소지역적 특성을 지닌 일본의 대표적인 산촌지역인 湯布院町의 보건복지전달체계 양상을 살펴봄으로써 향후 우리나라 농산촌지역의 보건복지전달체계 구축과정에서 고려해야 하는 다양한 내 ·외적 요인에 대한 예측을 위한 기초자료를 제공하기 위한 것이다. 결론적으로 본 연구는 湯布院町의 사례를 통하여 지금까지 농촌형 보건복지 전달체계를 구축함에 있어서 부족한 자원의 개발이나 접근성의 문제에 대한 논의는 있으나 토착주민간의 내부갈등에 패한 고려는 이루어지지 않다는 점을 고려할 때, 지역 내 권력구조의 유무와 그들간의 갈등양상이 농산촌지역 보건복지서비스체계 구축에 커다란 변수로 작용함을 실증적으로 보여주고 있다. This study is focused on the systems of health care and welfare service of Yufuin district where is one of Semimountainous Regions in the north Kyushyu that shows both and traditional power structure and small area's characteristics in own region. This study is expected it is able to implicate any basic data in forcasting various in-or outside factors required for constructing a health care and welfare system in Korean farming or mountain ones. In developing the health care and welfare system for agricultural communities, short resources development and service accessibility have concerned, but a community power structure, some conflicts between two traditional ruling families and so on are critically not considered. Finally, this study is concluded that the pattern of power structure and related conflicts in each group are being run as a major variable in constructing the health care and welfare system in semimountainous region.

低사이클 反引張을 받는 鋼棒의 圓孔노치底 크랙發生에 관한 硏究

孔基勳,吳世旭 東亞大學校 大學院 1981 大學院論文集 Vol.5 No.-

Many studies on fatigue crack propagation have been presented but the behavior of fatigue and its mechanism is not clarified enough yet, especially low cycle fatigue. Some experiments in this study were carried out by the method of changing stress amplitude under constant mazimum stress and low cycle pulsating tension fatigue testing on fatigue crack propagation at room temperature. Propagating cracks were observed with potical microscope on crack initiation and growth. The obtained results are summarized as follows: ⑴ Fatigue strength was higher in S-N curve as stress amplitude was decreasing, mean stress inereasing, too. ⑵ The initiation of fatigue crack was occured at 28~32% through the total fatigue life. ⑶ The summarized relationship between crack propagation rate and stress intensity factor of structural steel (SM45C) is as follows. The value of exponent m was 1.41-1.85. ⑷ Propagating crack in fatigue generally passed along grain boundaries and ferrite matrix, it passed around and branched cause of hard inclusion. ⑸ In the case of higher stress amplitude the shpae of crack propagation and fracture surface was intricate.