단치소요산이 구속 스트레스를 가한 흰쥐의 뇌내 catecholamine 함량변화에 미치는 영향

황귀서,이기선,박종형 한국환경독성학회 1998 환경독성보건학회지 Vol.13 No.2

To elucidate the preventive effect of Danchisoyosan (DS) on stress, we investigated the physiological change of rats which were applied immobilization stress. For immobilization stress, rats were placed in restrainer for 12 hours a day for 3 days. During application of stress, body weight of rats was measured. After sacrifice, 8 organs were taken for measurement of organ weight. Brain was sectioned into 4 parts that are Frontal Cortex, Corpus Striatum, Hypothalamus and Hippocampus. Each part was homogenated and its catecholamine and serotonin contents were measured with HPLC. In our study, stress mainly induced increase of concentration of neurotransmitters in brain without other significant physical change of rats. DS inhibited stress induced changes of neurotransmitter content in brain.

시간경과에 따른 안정화 이산화염소(Stabilized ClO₂)의 콩팥조직 부패억제에 대한 현미경적 연구

황규성,최기주,백두진,임도선 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

소취 및 소독제로서 사용되어온 안정화 이산화염소(S-ClO₂)의 조직에 대한 부패억제 효과를 확인하고자 본 연구를 수행하였다. 실험을 위해 8주령 SD계 흰쥐 콩팥(kidney)을 사용하였고, 안정화 이산화염소를 처리하지 않은 대조군과, 안정화 이산화염소의 분말과 수용액을 처리한 실험군으로 구분하여 광학 및 전자현미경으로 관찰하였다. 광학현미경 관찰 결과, 부패양상은 시간경과에 따라 토리, 보우만주머니 및 세뇨관을 구성하는 세포의 핵과 세포사이 경계가 불분명해지며, 세뇨관의 경우 신장되었다가 결국에 수축되었다. 대조군의 1일군부터 괴사(necrosis)가 시작되어 3일군 이후는 전체적인 조직 괴사로 형태를 구별하기 어려웠다. 실험군에서 3일군의 경우, 조직의 전반적 형태와 괴사정도가 대조군의 1일군과 유사하였다. 전자현미경 관찰 결과, 시간경과에 따라 세포소 기관 및 미토콘드리아의 부분적 붕괴로 시작되어 결과적으로 모든 세포내소기관이 붕괴되었다. 대조군의 1일군에서 세포소기관의 부분적 붕괴가 관찰되었으며, 실험군의 3일군에서 세포소기관 및 미토콘드리아의 부분적 붕괴 현상이 관찰되었다. 대조군의 3일군 이후에서는 세포소기관을 구별할 수 없었다. 이상의 연구 결과에서 37˚C, 습도 80±5%에서 안정화 이산화염소(S-ClO₂)가 부패와 변성을 억제하는 부패억제제로서 효과가 있고, 억제 정도는 실험군의 3일군이 대조군의 1일군에 해당하는 것으로 보아 최소 2일 정도의 부패억제 효과가 있는 것으로 확인되었다. The stabilized ClO₂ gas has been used for many years by the food industry as a strong oxidizing and sanitizing agent that has broad and high biocidal effectiveness. Therefore, “stabilized ClO₂” gas may be used in fields of disinfectant and sterilization. But, there have been few studies on the decomposition-inhibition effect of stabilized ClO₂ gas with passage of time. The main purpose of this study was to examine the decomposition-inhibition effect of stabilized ClO₂ gas and the morphological change of kidney by measuring of the light and electron microscope. Sprague-Dawley (SD) rats weighting from 230 gm to 250 gm were used as experimental animals. Under ether anesthesia, the right kidney of rat was obtained. Put each sample in 37˚C and humidity 80±5% incubator, we obtained each sample after 0 day, 1 day, 2 days, 3 days, 4 days and 5 days. We proceeded the observation of light and electron microscope. The results obtained in this study reveal that stabilized ClO₂ gas is an effective decomposition inhibitor until 2 days that was conducted at 37˚C and humidity 80±5% conditions.

A tetracosatetraene as larvicidal compound isolated from <i>Alpinia katsumadai</i>

Hwang, Ki Seon,Kim, Young Kook,Kim, Young Tae,Lee, Joongku,Park, Kee Woong ELSEVIER 2017 INDUSTRIAL CROPS AND PRODUCTS Vol. No.

<P><B>Abstract</B></P> <P> <I>Plutella xylostella</I> and <I>Mythimna separata</I> are destructive insect pests causing damages to agricultural products. In this study, a larvicidal compound was identified by bioassay-guided isolation of methanol extracts of <I>Alpinia katsumadai</I> Hayata, a plant belonging to the Zingiberaceae, against <I>P. xylostella</I> and <I>M. separata</I> larvae. Because many insects, including <I>P. xylostella</I> and <I>M. separate</I> larvae, cannot synthesize sterols, they use phytosterols by converting them to the sterols necessary for hormonopoiesis, and resulting normal development, growth and reproduction. Results of our oil droplet formation test showed that compound 1 inhibited foam cell formation in ox-LDL treated THP-1 cells, suggesting that this compound may be inhibiting the sterol transport in insects. The compound 1 with larvicidal activity was elucidated by spectroscopic analysis (<SUP>1</SUP>H NMR, <SUP>13</SUP>C NMR, and ESI–MS) as 2,3,22,23-tertrahydroxy-2,6,10,15,19,23-hexamethyl-6,10,14,18- tetracosatetraene. The 50% lethal concentration (LC<SUB>50</SUB>) values of compound 1 were 2μg/mL and 16.9μg/mL, respectively, against <I>P. xylostella</I> and <I>M. separata</I> larvae.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The novel larvicidal compound was identified from <I>Alpinia katsumadai</I>. </LI> <LI> The active compound was isolated by bioassay-guidance with some chromatography. </LI> <LI> The active compound was elucidated by spectroscopic analysis with <SUP>1</SUP>H NMR, <SUP>13</SUP>C NMR, and ESI–MS. </LI> <LI> The active compound was screening by foam cell formation test with THP-1 cells. </LI> <LI> The active compound has larvicidal effect against <I>Plutella xylostella</I> L. and <I>Mythimna separate</I>. </LI> </UL> </P>

Piperolein B and piperchabamide D isolated from black pepper (<i>Piper nigrum</i> L.) as larvicidal compounds against the diamondback moth (<i>Plutella xylostella</i>)

Hwang, Ki Seon,Kim, Young Kook,Park, Kee Woong,Kim, Young Tae WILEY 2017 PEST MANAGEMENT SCIENCE Vol.73 No.8

<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P>There is growing demand for the development of alternative pest control agents that are effective as well as non‐toxic to human health and the environment. Plant protection products derived from plant extracts are an eco‐friendly alternative to synthetic pesticides. The aim of this study was to identify larvicidal compounds isolated from a natural source against <I>Plutella xylostella</I> L. In a larvicidal activity assay, several solvent fractions from the methanol extract of <I>Piper nigrum</I> L. fruit showed larvicidal effects against <I>P. xylostella</I>.</P><P><B>RESULTS</B></P><P>Screening results indicated that chloroform extract was the most effective against <I>P. xylostella</I> larvae. Two compounds with insecticidal activity in the chloroform fraction were identified as piperolein B and piperchabamide D by spectroscopic analyses, including mass spectrometry and NMR, and by comparison to published data. At applications of 0.1 mg mL<SUP>−1</SUP> concentration, piperolein B and piperchabamide D, respectively, induced 96.7 ± 5.8% and 79.2 ± 16.6% mortality rates of <I>P. xylostella</I> larvae 4 days post‐application.</P><P><B>CONCLUSION</B></P><P>Our results demonstrate that piperolein B and piperchabamide D isolated from <I>P. nigrum</I> are the major constituents of the extract demonstrating insecticidal properties for the control of <I>P. xylostella</I> larvae. These plant‐derived compounds should become useful alternatives to synthetic chemicals after studying their insecticidal mechanisms. © 2017 Society of Chemical Industry</P>

Occurrence and distribution of weed species on horticulture fields in Chungnam province of Korea

Hwang, Ki Seon,Eom, Min Yong,Park, Su Hyuk,Won, Ok Jae,Lee, In Yong,Park, Kee Woong The Ecological Society of Korea 2015 Journal of Ecology and Environment Vol.38 No.3

A survey of weed occurrence was conducted to identify problematic weed species in a horticultural crop field to get basic information for effective weed control. Surveys of weed species occurring in horticultural crop fields (garlic, onion, red pepper and Chinese cabbage) were conducted in Chungnam province of Korea from April to October in 2014. A total of 516 sites of the 17 regions were identified as having 114 weed species belonging to 32 families. The most dominant weed species in the horticultural crop fields were Chenopodium album var. centrorubrum (8.83%), followed by Digitaria ciliaris (5.71%), Conyza canadensis (5.46%) and Capsella bursa-pastoris (4.67%). Specifically, as a result of this study, the occurrence of 35 species of exotic weeds, such as Chenopodium album and Taraxacum officinale, were confirmed. Almost 68% of the investigation sites was determined under dominance value 1 (range of cover < 10; numerous individuals) by Braun-Branquet cover-abundance scale, indicating a proper weed control in horticultural crop field. As a result of scientific and technological advances, an improved cultivation method is changing the weed occurrence in agricultural land. Additional research needs to be undertaken for the development of weed control methods through such periodic monitoring of occurrence of weeds.

Damage on Lemonbalm by Chrysolina exanthematica

Seon-u Choi,Yoon Ki Hong,Hoi-Chun Lim,Jeong-Man Kim,Chang-yun Hwang 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

Field survey were conducted in to evaluate the insect damage to growing Lemonbalm(Melissa officinalis). Lemaonbalm, belonging to the Lamiaceae family, is a smelling perennial shurb which is possible to grow in Korea. To date, there has been minimal research regarding plant culture, pest insect occurrence and management research about herbal plants in Korea. Meanwhile, it grows for processed food and cosmetics in Jellabukdo, and necessity to pest management is on the rise. Chrysolina exanthematica(Coleoptera: Chrysomelidae) was realized not pest but general insect in Korea. But, in this survey, it is found to pest about lemonbalm. Damage period was concentrated in April. Rest period was decreased in density. At size of Chrysolina exanthematica, larva is 7.0*3.6mm, pupa is 8.3*5.3mm, and adult is 9.7*6.7mm. In farm surveying about Chrysolina exanthematica host preference of 5 species herb plants, Lemonbalm has great preference but, Oregano has no preference. Surveying leaf damage about 6 species in Lamiaceae family in lab., Lemonbalm belonging to Melissa genus has great damage, Some species in Mentha genus have little damage. And, Oregano in Origanum genus no damage. In Growth characteristics of healthy and damage plant, plant length on damage plant is decreased 41.3%, number of tiller is decreased 67%. Fresh and dry plant weight of damaged plant is decreased 66.7%, 64.2% compared with healthy plant. Because damage of insect can influence on plant main contitutents, we analysed the constitutent parts of extracts from supercritical fluid extraction system using gas chromatography/mass spectrometer.

ZigBee의 영상 전송을 이용한 침입자 탐지 시스템 구현

황선기(Seon-Ki Hwang),정의수(Eui-Su Jeong),권오학(Oh-Hak Kwon),이상구(Sang-Gu Lee) 한국정보과학회 2009 한국정보과학회 학술발표논문집 Vol.36 No.2D

본 논문에서는 최근 보안에 대한 중요성이 증가됨에 따라 유비쿼터스 환경을 위한 기술을 구현하기 위해 가장 주목받고 있는 기술 중의 하나인 ZigBee를 이용해 영상 전송이 가능한 침입자 탐지 시스템을 구현한다. 구현된 시스템은 침입자 및 상황 탐지를 위해 적외선 센서, 온도, 습도, 조도 센서 등을 사용하고, 방문자를 인식하는 RFID리더기, 적용된 공간의 이미지를 제공하는 카메라 모듈 등을 사용한다. ZigBee 프로토콜을 사용하여 센서 네트워크를 구성하여 윈도우 기반의 프로그램 상에서 제어하는 침입자 탐지 시스템을 구현한다. 이 시스템은 앞으로 저가격, 저전력의 ZigBee 기술의 특징으로 보안성, 편리성, 사용의 용이성 등을 제공할 것으로 기대된다.

인덕대학 가상 교육 체계 구축에 관한 연구

허기중 ( Ki-joong Huh ),김혜선 ( Hye-seon Kim ),김한응 ( Han-eung Kim ),황선철 ( Seon-cheol Hwang ),최준기 ( Joon-ki Choi ) 한국고등직업교육학회 2002 한국고등직업교육학회논문집 Vol.3 No.4

This paper describes a method to design and construct a virtual education system(VES) of Induk Institute of Technology. Four main concepts were considered to design our VES. The first concept is the designer of lectures that is able to establish a strategy for effective lectures. The second is the consideration for students who should study hard and check processing status themselves. The third is how to design and compose virtual educational contents. And the last concept is how to set up the central systems and how to manage them. Our VES was designed and is being consturcted based on the above concepts successfully.

충남지역 배추재배지 발생잡초 분포특성

황기선(Ki Seon Hwang),엄민용(Min Yong Eom),박수혁(Su Hyuk Park),원옥재(Ok Jae Won),서수정(Su Jeoung Suh),이인용(In Yong Lee),박기웅(Kee Woong Park) 충남대학교 농업과학연구소 2014 농업과학연구 Vol.41 No.4

This study was conducted in order to utilize the basic data for weed control by surveying the occurrence of weed species. Total 63 sites of upland Chinese cabbage fields in Chungnam Provinces were investigated. The result of survey, 71 weed species in 25 families were identified and classified to 39 annuals, 16 biennials and 16 perennials. Based on the occurrence ratio, the most weed species belonged to Compositae (20 species). 8, 6 and 5 weed species belonged to Poaceae, Cruciferae and Polygonaceae, respectively, and these 10 weed species in the most six families accounted for 50% of total weed occurrence. The most dominant weed species in upland Chinese cabbage fields were Portulaca oleracea (8.07%), followed by Digitaria ciliaris (7.54%), Rorippa palustris (6.44%), Chenopodium album (5.73%), Echinochloa crus-galli (5.02%) and Cyperus amuricus (3.95).

영상분할 기법을 이용한 차량 번호판 인식 구현에 관한 연구

황선기(Seon Ki Hwang),김태우(Tae Woo Kim),강용석(Yong Seok Kang) 산업기술교육훈련학회 2017 산업기술연구논문지 (JITR) Vol.22 No.4

This paper presents novel methods of recognizing license plates of passing vehicles outdoors. In particular, the proposed method is much robust for inclined plates caused by the changes of camera placement. To ac-quire fine images of quickly passing vehicles under a wide range of illumination conditions, we developed a sensing system having superb characteristics. This study a vehicle license plate recognition system by Image Segmentation Methods, which works on license plate image at the another time. We propose a system that can obtain a clearer and wider dynamic image range by combining a pair of images simultaneously captured by two CCD cameras. As the experimental results obtained from the existing system environment recognition rate of about 86.5%, and the recognition rate of the proposed system is 93.5%. Approximately 7% of the recognition rate can be seen to be rising.