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        Identification of Nucleolin as a Lipid-Raft-Dependent Beta1-Integrin-Interacting Protein in A375 Cell Migration

        Jiajia Bi,Xianlu Zeng,Ruifei Wang,Yue Zhang,Xiaoqing Han,Khamal Kwesi Ampah,Wenguang Liu 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.6

        Lipid rafts are related to cell surface receptor function. Integrin is a major surface receptor protein in cell adhesion and migration on the extracellular matrix (ECM). Here, we showed that lipid rafts played a critical role in human melanoma A375 cell spreading and migration on fibronectin; an important component of the ECM that interacts with 1 integrin. We found that the disruption of lipid rafts did not markedly inhibit the expression and activation of 1 integrin. By coimmunoprecipitation and mass spectrometry, we investigated the influence of lipid rafts on the 1 integrin complex and identified nucleolin as a potential lipid-raft-dependent 1-integrin-interacting protein. Upon confirmation of the interaction between 1 integrin and nucleolin, further studies revealed that nucleolin colocalized with 1 integrin in lipid rafts and raft disruption interrupted their association. In addition, knockdown of nucle-olin markedly attenuated A375 cell spreading and migration on fibronectin. Taken together, we demonstrated that nucleolin is a critical lipid-raft-dependent 1-integrin-inte-racting protein in A375 cell spreading and migration on fibronectin.

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