Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

산업용 전력시스템의 전압저감내성 해석 및 대책

유인근,지화수 國立 昌原大學校 産業技術硏究所 1999 産技硏論文集 Vol.13 No.-

This paper describes the causes of voltage sags in industrial plants, their impacts on equipment operation, and the possible countermeasures. The voltage sags analysis of power systems under faulted conditions provides very important information regarding the sensitivity of different types of industrial equipment, adjustable speed drive controls, programmable logic control, motor contactor and so on. The ability to predict voltage sag characteristics offers a unique opportunity to evaluate alternate configurations and prevent problems with optimum supply and ride-though specifications. Currently, the voltage sags are facing an increasing concern of industrial plants due to increasing automation facilities as electronic controllers, which are more sensitive to voltage sags than other loads. Several countermeasures are chosen and simulated in order to improve voltage sags of industrial plant and the simulation results are discussed in detail.

연안부근 복잡지형의 대기유동장 수치실험 1 : 선형이론을 이용한 국지순환모형의 타당성 검토 A Verification of Propriety of Local Circulation Model Using the Linear Theory

이화운,김유근,정우식 한국환경과학회 1999 한국환경과학회지 Vol.8 No.5

A sea/land breeze circulation system and a regional scale circulation system are formed at a region which has complex terrain around coastal area and affect to the dispersion and advection of air pollutants. Therefore, it is important that atmospheric circulation model should be well designed for the simulation of regional dispersion of air pollutants. For this, Local Circulation Model, LCM which has an ability of high resolution is used. To verify the propriety of a LCM, we compared the simulation result of LCM with an exact solution of a linear theory over a simple topography. Since they presented almost the same value and pattern of a vertical velocity at the level of 1 ㎞, we had a reliance of a LCM. For the prediction of dispersion and advection of air pollutants, the wind field should be calculated with high accuracy. A numerical simulation using LCM will provide more accurate results over a complex terrain around coastal area.

뉴로-퍼지 논리를 이용한 12경락의 양도락진단 시스템의 구현

김병화,한권상,이우철,김성현,최해성,안현식,김도현 대한전자공학회 1997 秋季綜合學術大會 論文集 Vol.20 No.2

율촌 LNG 발전소의 공냉식 복수기 사용에 따른 열확산 수치모의

김유근,이화운,문윤섭,황미경 부산대학교 환경문제연구소 2000 環境硏究報 Vol.18 No.-

In this study, thermal diffusion caused by using air cooled condenser at LNG power plant in Yulchon was simulated through in numerical method (Matsuno scheme). The input data of model was used to seasonal wind components (u, v) of automatic weather system (AWS) and seasonal temperature ranges between inflow and outflow of opening air. The aim of this study was twofold : (1) to investigate sensibility analysis of thermal diffusion coefficient : (2) to simulate diffusion of heat source through the calculated effect height for season and stability variations. These sensibility analyses were performed in detail using two case studies one is to apply horizontal and vertical thermal diffusion coefficient as 50 m-ls-1 and 10 m-ls-1, the other is 30 m-ls-1 and 10 m-ls-1 respectively. It is well known that the sensibility for thermal diffusion coefficient is low owning to very similar results. Consequently, thermal diffusion for the first case is simulated by using effect height for season and stability variation. The temperature is under 1℃ at surface whereas that is high at near the center of effect height.

해륙풍 수치모의의 타당성에 관한 연구

이화운,김유근,임주연 부산대학교 환경문제연구소 1995 環境硏究報 Vol.13 No.-

The accuracy and applicability of numerical simulation model for land-sea breezes are studied by the computative study between analytical model and numerical simulation results. In the model, Defant's equation which has analytical solution was selected. Both numerical and analytical solutions represented well land and sea breezes under the condition of various diffusion coefficients and hydrostatic and nonhydrostatic conditions. By the results, the numerical simulation method was proved as an appliable technique to simulate accurate land-sea breezes.

느타리버섯(Pleurotus ostreatus) 재배균상에 오염되는 Rhizoctonia solani 유사균의 특성

이영근,장화형,김진규,김재성,이기성 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.4

느타리버섯(Pleurotus ostreatus) 재배균상은 흔히 토양, 공기 중 곰팡이에 의해 오염되어 자실체 생산량이 감소되곤 한다. 느타리버섯 재배균상에서 3종류(Rs-1, RS-2와 RS-3)의 오염균주를 분리하여 internal transcribed spacer(ITS)의 염기서열을 분석하였다. 이들의 ITS염기서열은 식물 병원성 사상균의 일종인 Rhizoctonia solani와 매우 높은 상동성을 보였다. 이들 오염균주들의 영양배지와 lignin 배지에서 균사체 생장율은 느타리버섯보다 각각 수배 및 수십배나 높았다. 세포외분비효소의 활성도는 오염균주들의 경우 느타리버섯과 비교하여 매우 낮은 활성도를 보였다. 느타리버섯과의 혼합배양 결과 오염균주들은 느타리버섯의 균사체 생장 및 자실체 형성을 심하게 저해하였다. The contamination of air- and soil-borne microbes could occur and result in the reduction of fruiting body formation during cultivation of Pleurotus ostreatus. Three contaminants(RS-1, RS-2 and RS-3) were isolated and showed higher homology to the plant pathogenic fungus, Rhizoctonia solani by internal transcribed spacer(ITS) analysis. The rates of their mycelial growth on the lignin specific medium and complete medium were several times higher than those of P. ostreatus. The activities of the extracellular enzymes of three contaminants were lower than those of P. ostreatus. During oyster mushroom cultivation(P. ostreatus) together with these contaminants, the mycelial growth and fruiting body formation of P. ostreatus was seriously repressed.

김수영 시의 관념성

이근화 국어국문학회 2004 국어국문학 Vol.- No.137

In Kim su-young's poetry, there are many non-poetic words and conceptional expressions ; abstract ideas, slang expressions, naturalized words and etc. Some researchers regard it as various one, others disordered. But just making a list of vocabulary means nothing. We could recognize that Kim su-young used words and phrases to achieve the new and vivid meaning, especially in the aspect of using ideological words represented in his poetry. So we must have a thorough grasp the effect and direction of using poetic words. Everyone says that the blooming of the flower is beautiful and miraculous. However, when Kim, su-young had witnessed the moment of blooming, he discovered a popular fallacy in the event and broke the usual diction in his poem "꽃(flower)". In his poem, a flower comes to have whole different meaning from the usual diction. It becames 'a firm flower blooming in disorder'. Kim, su-young frequently raise a question of the meaning and importance of writing in our daily life. In a work titled "눈(snow)", he looked at the snow falling and reflected a poem writing at the same time. Ultimately, Kim, su-young represents that not the ruins but the snow-falling ruins imply the reality. Also in "절망(Despair)", lots of ideological words were repeated and juxtaposed on the surface in the poems. However, the repetition and justaposition of ideological words let us obstinately resist from the reality. In a work titled "구라중화(gladiolus)", Kim, su-young introduced a method of the infinitive mood. By using the infinitive mood, he has denied an ideality of ideological words. He repeat the ideological dictions to identify the substance of a flower but he failed. But at the point of failure, he came up to see the shadow of a flower and surroundings of it. Through this process, he was confronted with death and he secure the power to overcome his time. Kim, su-young used many ideological words, but he did not ignore details of the reality. He gives a shape to ideological words in his poems, and poetic diction achieves a concrete meaning. He did not take shelter behind transcendental rhetorics and did not hide himself behind a poetic diction. He found out a distortion in daily lives and discolsed a suppression in our society.

중규모 바람장 해석을 위한 Fine Mesh Model의 구성

이화운,김유근,이부용,오은주 부산대학교 기초과학연구소 1995 부산대학교 기초과학연구소 연구논문집 Vol.15 No.-

To predict reasonably the movement and the concentration of the pollutants in the coastal area. A simulation model should be prepared considering detail topography with land-sea and the urban effects, and the resolution near the source. The explicit method can not be applied due to the unstability of the numerical calculation in high horizontal-grid resolution, while the ADI scheme satisfied with the high horizontal grid resolution and can be used in the fine mesh system which shows the detail topography, atmospheric flow. The ADI method which studied the high horizonal grid resolution was excellent. The two dimentional model used in the study using ADI method is proved as a reasonable model to predict the wind field in any small scale area including mountainous coastal urban area.

류마티스성 심장판막질환, 우심방혈전 및 대동맥 협착증과 합병된 만성 혈전색전성 폐동맥고혈압 1예

유근배,심준,임양희,이진화,신길자 梨花女子大學校 醫科大學 醫科學硏究所 1998 EMJ (Ewha medical journal) Vol.21 No.4

폐동맥 혈전색전증은 비교적 드문 질환으로 주로 심부 정맥혈전에서 발생하는 것으로 알려져 있으며 류마티스성 심장판막질환을 가진 경우 합병되는 우심방 혈전이 드물게 폐혈전색전증의 원인이 될 수 있는데, 저자들은 대동맥의 동맥경화성 협착증을 동반한 류마티스성 심판막질환 환자에서 우심부전 및 우심방혈전에서 발생한 만성 혈전색전성 폐동맥고혈압1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Most pulmonary thromboemboli arise from the deep vein thrombosis, which have complete clinical and at least near complete roentgenographic and angiographic resolution within four to six week of the acute event. But chronic pulmonary hypertension and cor pulmonale from unresolved pulmonary embo-lism complicate acute embolic episode with a frequency of less than 1 percent. Rarely pulmo-nary thromboemboli can result from right atrial thrombi. We experienced a case of chronic thromoboembolic pulmonary hypertension, which wrose from right atrial thrombi in the patient with rheumatic valvaular heart disease and thoracic aorta atherosclerotics stenosis.