Interleukin-13 Increases Podocyte Apoptosis in Cultured Human Podocytes

Lee, Keum Hwa,Oh, Ji Young,Seong, Su-Bin,Ha, Tae-Sun,Shin, Jae Il Korean Society of Pediatric Nephrology 2018 Childhood kidney diseases Vol.22 No.1

Purpose: Podocytes are important architectures that maintain the crucial roles of glomerular filtration barrier functions. Despite this structural importance, however, the mechanisms of the changes in podocytes that can be an important pathogenesis of minimal change nephrotic syndrome (MCNS) are not clear yet. The aim of this study was to investigate whether apoptosis is induced by interleukin (IL)-13 in cultured human podocytes. Methods: Human podocytes were treated with different IL-13 doses and apoptotic cells were analyzed using terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL assay) and fluorescence-activated cell sorting (FACS). Results: The IL-13 increased the number of TUNEL-positive cells in a dose-dependent manner at 6 and 18 hours (P<0.05 and P<0.05, respectively). The apoptosis rate was appeared to be increased slightly in the IL-13-stimulated podocytes (8.63%, 13.02%, and 14.46%; 3, 10 and 30 ng/mL, respectively) than in the control cells (7.66%) at 12 hours by FACS assay. Conclusion: Our study revealed that IL-13 expression may increase podocyte apoptosis. Blocking the IL-13 signal pathway can potentially play an important role in regulating the apoptosis of podocytes.

삼황사심탕(三黃瀉心湯)이 난황 알부민으로 유도된 알레르기 Mouse모델에서 항알레르기 효과

최종환 ( Chong Hwan Choi ),금선오 ( Seo Oh Keum ),이세원 ( Se Won Lee ),김일현 ( Il Hyun Kim ),이하일 ( Ha Il Lee ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2014 한방재활의학과학회지 Vol.24 No.3

Objectives In this study, we investigated the inhibitory effects of Samhwangsasim-tang (S.H) on the allergic response caused by ovalbumin(OVA) sensitization and challenge in BALB/c mice. Methods The experimental animals were divided into five groups; 1) normal as negative control, 2) OVA-sensitized mice, 3) OVA-sensitized mice treated with 200 mg/kg of S.H 200, 4) OVA-sensitized mice treated with 400 mg/kg of S.H 400, and 5) OVA-sensitized mice treated with 5 mg/kg of Dexamethasone (Dex). Antigen sensitization for allergic mouse model was performed with twice injection of OVA for 2 weeks. After secondary injection, S.H was administrated orally into mice every day for 13 days and the inhibitory effect of S.H on allergic responses was evaluated. Results Treatment of S.H into allergic mice reduced significantly ear edema and infiltration of immune cells in ear tissues induced with OVA challenge in a dose-dependent manner. S.H reduced significantly the serum levels of Total Immunoglobulin(Ig)G and IgE, and particularly inhibited the production of OVA-specific IgE, but not OVA-specific IgG. The serum level of proinflammatory cytokine TNF-α and Th2-associated cytokine IL-4 also were significantly decreased by S.H adminstration in a dose denpendent manner. S.H attenuated OVA-induced secretion of IFN-γ, but not IL-12 which is a cytokine inducing the development of Th1 cells. It also reduced significantly the secretion of IL-4, which is a cytokine inducing the development of Th2 cells, after splenocytes were stimulated with OVA. However the secretion of IL-5 and IL-13 was influenced weakly or a little. Conclusions These results indicate that S.H could reduce the allergic response through inhibition of antigen-specific IgE and Th2-inducing cytokines. It suggest that S.H may be available clinically for the treatment of allergic patients. (J Korean Med Rehab 2014;24(3): 71-85)

8주간의 유산소성 체조활동을 통한 뇌병변 장애인의 체력 및 염증반응지표 변화

허금 ( Keum Heo ),김광준 ( Kwang Jun KIm ),김권일 ( Kwon Il Kim ),박동호 ( Dong Ho Park ),전태원 ( Tae Won Jun ),송욱 ( Wook Song ) 한국특수체육학회 2009 한국특수체육학회지 Vol.17 No.4

본 연구의 목적은 뇌병변 장애인을 대상으로 8주간의 유산소성 체조활동을 실시하여 신체조성, 체력 및 염증반응지표의 변화에 대하여 알아보는 것이다. 본 연구의 피험자는남·녀 뇌병변장애 중 대표적인 뇌졸중 장애인 17명을 대상으로 시작되었지만 9명이 중도포기하여 최종 8명이 참가하였다. 운동 프로그램 참여 전 사전 검사를 실시하였으며, 기본적 변인으로 신장, 체중, Body Mass Index(BMI), 안정시 심박수, 안정시 혈압을 측정하고, 체력 변인으로는 체간 굴곡과 신전, 견관절 굴곡과 신전의 유연성과 악력을 통한근력, 폐활량을 통한 폐기능을 측정하였다. 또한 혈액성분으로는 혈중지질, 그리고 심혈 관질환 위험인자인 염증반응지표로서 C-reactive protein(CRP), Interleukin-6(IL-6), Tumor Necrosis Factor-α(TNF-α)를 분석하였다. 8주 유산소성 체조활동 프로그램은 매회50분씩 주 2회 실시하였으며, 운동강도는 운동자각도(RPE) 수준 13으로 유지하였다. 뇌병변 장애인의 유산소성 체조활동 프로그램 참여 후 운동의 효과를 검증한 결과 전체 집단에서 안정시 수축기혈압과 이완기혈압이 유의하게 감소하였다(p<.01). 또한 체력 요인에서는 체간 굴곡과 신전(p<.05), 건측의 견관절 굴곡과 신전(p<.01), 환측의 견관절 굴곡(p<.05)이 유의하게 증가하였다. 혈액 성분의 분석 결과 혈중지질에는 유의한 변화가 나타나지 않았으며, 염증반응지표의 경우 IL-6가 유의하게 감소하였고(p<.05), 유의하지는 않으나 CRP도 감소하는 경향을 나타냈다(p<.088). 결론적으로, 지속적이고 규칙적인 유산소성 체조활동은 뇌병변 장애인의 안정시 심박수와 혈압 및 체력을 개선시키는 효과가 있을 뿐만 아니라 심혈관질환의 위험요소로 알려져 있는 염증반응지표를 개선하는 데에도 긍정적인 영향을 주는 것으로 나타났다. The purpose of this study was to analysis the effect of aerobic gymnastic exercise for eight weeks on physical fitness and inflammatory markers which are known as risk factors for cardiovascular diseases in hemiplegic disabled after cerebral stroke. For this purpose, stroke patients(n=8) participated in aerobic exercise training 2 days/week, 50 mins/day, for 8 weeks and maintained at 13 level of RPE. Body composition, resting heart rate and blood pressure were measured before physical fitness test. Trunk flexion/extension, shoulder flexion/extension, grip strength, pulmonary function were measured as physical fitness test. Blood sample of 15ml was drawn from antecubital veins for measuring inflammatory markers(c-reactive protein, interleukin-6, tumor necrosis factor-α). The result of this study is as follows: Resting blood pressure decreased significantly after 8-week aerobic exercise training in total stroke patients (p<.01). After completion of the aerobic exercise program, trunk extension/flexion(p<.05), shoulder extension/fexion(p<.01), were significantly improved in total stroke patients. There was no significant improvement on blood lipid profiles in total stroke patients. However, IL-6 was significantly improved in stroke patients after 8-week aerobic exercise(p<.05). In conclusion, These findings suggests that regular aerobic gymnastic exercise decreases resting heart rate and blood pressure, and improves physical fitness, inflammatory markers which are known as a risk factor of cardiovascular diseases in person with disability occurred due to hemiparetic stroke

NF-κB 억제를 통한 대회향(大茴香)의 항염증효능

금수연,박상미,제갈경환,황보민,조일제,박정아,김상찬,지선영,Keum, Soo Yeon,Park, Sang Mi,Jegal, Kyung Hwan,Hwangbo, Min,Cho, Il Je,Park, Chung A,Kim, Sang Chan,Jee, Seon Young 대한한의학방제학회 2016 大韓韓醫學方劑學會誌 Vol.24 No.4

Objectives : Illicium verum Hook. f. has been known to possess antimicrobial, antioxidant, antifungal, anti-inflammatory, insecticidal, analgesic, sedative, convulsive activities, it has been rarely conducted to evaluate the immuno-biological activity. The present study was examined to evaluate the anti-inflammatory effects of the Illicium verum Hook. f. water extracts (IVE) in vivo and in vitro. Methods : Cell viability was measured by MTT assay. The relative levels of NO were measured with Griess reagent. iNOS, COX-2, $NF-{\kappa}B$ and target proteins were detected by immunoblot analysis, and levels of cytokines were analyzed by ELISA kit. Anti-edema effect was determined in the carrageenan (CA)-induced paw edema model in rats. Results : All dosages of IVE used in MTT assay had no significant cytotoxicity. The increases of NO production and iNOS expression were detected in LPS-treated cells compared with control. However, these increases were attenuated by treatment with IVE. Also, IVE reduced the elevated production of $TNF-{\alpha}$, $IL-1{\beta}$ and IL-6 by LPS. IVE inhibited the $p-I{\kappa}B$ and translocation of $NF-{\kappa}B$ to nuclear. Furthermore, IVE significantly inhibited the increases of hind paw swelling, skin thicknesses and inflammatory cell infiltrations induced by CA injection. Therefore, IVE will be favorably inhibited the acute edematous inflammations. Conclusion : These results provide evidences that anti-inflammatory effect of IVE is partly due to the reduction of some inflammatory mediators by suppression of $NF-{\kappa}B$ pathway.

資産再評價制度에 관한 硏究

李一琴 진주여자전문대학 1991 論文集 Vol.14 No.-

Modern company successively carries out its management activity under the preposition of going concern. However today's money value has kept changing and under the age of high inflation, the outstanding difference between cost and market price gave us the opportunity on revaluation of assets. In this paper, I surveyed the changing process, the effect, the point at issue and prospects of asset revaluation. In surveying of the changing process asset revaluation of Korean enterprise, we could make out the process of its act. Through the effect of asset revaluation in enterpries, I examined the accelerated effect of depreciation, the negative effect of financial frame of enterprise. The point at issue and prospects of asset revaluation are as fellows ; such as taxation of revaluation, unadmitting under revaluation cost, income accounting of transferation on revaluation asser, reconcilation of special cases, the deficit of constructual creteria, determination of scrape useful life on revaluation asset, the manipulation possibility of accounting number and voluntariness, addition issue of accounting number, etc Revaluation of asset actually raise the book value of private enterprise or corporation asset. Korean revaluation system of assets can protect the enterprise out of excessive taxation burdern and high divide excluding fictitious profit under the correct calculating depreciation cost and every enterprise improve its financial frame and maintain real capital merit, and encounter various criticism concerning the difficulties of time-lag comparison.

轉換社債의 會計處理와 財務的 評價에 관한 硏究

李一琴 진주여자전문대학 1993 論文集 Vol.16 No.-

In the above, this dissertation studied Accounting process and financial Evalument, convertible bond which has good advatage that mediates capital dement and Capital supply amicably is enterprise financial mathod and investment method. Convertible bend is mixed securites that was mixed stock and bond. in capital market is histerical Out put in capital supply method. For exanples We pereted that capital supply by convertible bond is filted to the enterprise that has good growth. It is very important to decid accrual time and convertible bond price in the case that the enterprise has high growth float convertible bond. At that case convertible bond price is according to the standard price of floating Points. This dissertation noticel this characte of convertible bond and actual nacessity theories. This Payer first consideraled fund mental about convertible bond. second applicated these theories to the assessmet of convertible bond. Third recongoniged that the value of convertible that endaned Convertible is very important especially. In the case high growth enterprise, and this point forth Convertible bond fixed market rate of interst and enterprise rate of growth. there fore, Capital supply by convertible bond reduce the cost of Agency that relaled to owner management duty extrinsic important and the theor basis deperd up on this. Becoase in case of that Full owner management supply the enternal quity, for maximige the utility he increase the convertible bond of duty extrinsic inportation owner management who has the enter prise that related to this in case of marketing Value price is dowve, share the cost At this point float of convertible bond reduction of Agency expence. It is desirable for owner who Speculate the real state of this enterprise to floot the convertible. bond, and for the decision of time and Convertible bond Price of convertible bond it is needful to afess the financial Evalument strictly.

財務情報의 有用性에 관한 實證的 硏究

이일금 진주여자전문대학 1994 論文集 Vol.17 No.-

活動基準原價計算에 관한 硏究

李一琴,全雄哲 진주여자전문대학 1997 論文集 Vol.20 No.-

The inadequacies of exiating cost accounting system have recently been the focus of much researches, that is. discussion on disadvantages of traditional costing systems(TCS). In this viewpoint this study reviews advatages of activity based costing(ABC) and then design and implementation of ABC. Advantages of ABC is that TCS have many failings - in particular, their inability to repore acequate cost informations for managing orgarnizations. ABC can provide a better understanding of how to reduce costs by managing the activities that cosume resources. One key to the application of ABC is a clear understanding of the physical activies represented by the structures of a particular ABC system. This study identifies design and implementation and theoretical suructures of ABC that have three elements as activities; activity centers, cost drivers, and resource categories

악안면 영역에 발생한 혈관종 환자의 외과적 치험례

김일규,박형준,최진호,장금수,김왕식 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

Hemangiomas are benign tumors in blood vessels that occur by traumatic or congenital origin. Lesions are appeared early in life, with 73% present at birth and 85% developing by the end of the first year. Head & neck hamangiomas represent about 25%∼33% of all hemangiomas. Kaban & Mulliken divided vascular birthmarks into two major categories : hemangioma and vascular malformation. Case 1 was diagnosed as cavernous hemangioma and the mass was excised completely and defected space was reconstructed with fat tissue. Case 2 was diagnosed definitely as traumatic A-V malformation by post-op histopathological examination and the mass was excised completely. This report describes two cases that were successfully treated by complete surgical excision for hemangioma in maxillofacial region.

高齡者의 生活課題와 學習課題의 探索

洪根一 聖心女子大學校 1990 論文集 Vol.22 No.-