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- 저자 : Keeyoeup (검색결과 3 건)
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Micropropagation of Ornamental Plants using Bioreactor System
Keeyoeup Paek,Hahn, Eun-joo,Heo, Jeong-wook,Son, Seong-ho 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-
Several types of bioreactors were incorporated to verify the effectiveness for mass culture of ornamental plants. Protocorm-like bodies (PLBs) were proliferated from flower stalk of Phalaenopsis by using bioreactors. Hyponex medium containing 1% potato and 0.1% activated charcoal supplied by ebb-and-flow method was investigated at the best for PLBs production (17 or more per PLB). The result partially suggested the possibility for mass propagation of PLBs through bioreactor system. Several cultivars of Lilium were successfully mass propagated using different types of bioreactors. In the case of 'Marcopolo', the growth of bulblets in balloon type bubble bioreactor (BTBB) was nearly 10 folds faster than that of the solid medium. When 120 single-nodal explants (about 1 cm in length) were cultured for 8 weeks under 16hr light condition, more than 20,000 shoot cuttings of virus-free potatoes could be obtained in 20 L sized BTBB. The percentage of ex vitro rooting of the cuttings during weaning stage revealed as more than 95% under 50% shade. Similar results were obtained from the culture of single-nodal explants using bioreactor in chrysanthemum and sweet potato. Based on our above results, we suggest that large-scale cultures of plant cell, tissue and organ using bioreactor system should be quite a feasible approach in terms of increase of multiplication rate and reduction of production cost when compared with conventional plant tissue culture method.
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허정욱,이춘우,백기엽 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-
이상으로, 발광다이오드를 광원으로 하는 자연광 이용형 보광장치는 광강도가 매우 낮은 장마기와 같은 광환경이 불량한 시기에 적절히 이용하므로서 식물체의 엽면적 증대, 왁스와 같은 특정성분 증량 및 분지등의 생장이나 형태형성에 효과적인 영향을 미칠 수 있는 실용적인 보광시스템으로서의 이용가능성이 검토되었다. 또한, 두종류의 관엽식물에 있어서 약간의 생장 및 형태형성에 있어 상이한 결과를 나타내었으나, 보광을 하지 않은 자연광구에 비하여 저광강도의 발광다이오드를 보광 조사하므로서 두 관엽식물의 생장이나 이상적인 형태형성이 이루어지는 것을 알 수 있었다. 금후, 관엽식물 뿐만 아니라 다른 식물 종을 대상으로 발광다이오드를 광원으로 하는 자연광 이용형 광조사 시스템을 광원으로 하는 연구가 활발히 진 The effect of supplemental lighting providing by Light-Emitting Diodes at daytime on growth of two ornamental plants was investigated. 'Camella' Dieffenbachia amoena and 'Melany' Ficus pumila were grown under different supplemental lights of blue, red, or blue plus red for 50days. The supplemental lights with 30㎛ol m-2 s-1 in photosynthetic photon flux were established in greenhouse which controlled at 27.5℃ air temperature and 75% relative humidity in average from 15th June to 4th August. Fresh weight of 'Camella' was the greatest in the supplemental treatment of blue plus red light, although there was no significant difference in dry weight. Higher plant height was shown in the mixture light of blue plus red and in control of natural lighting. The fresh and dry weights of 'Melany' were significantly higher in the mixture treatment than in control without any supplemental light. Number of unfolded leaves of 'Camella' and 'Melany' was increased in the mixture treatment. Net photosynthetic rate (NPR) of 'Camella' was the greatest in the mixturelight and control, and the red alone and mixture light treatments gave the highest NPR in 'Melany'. Wax content per leaf of blue plus red treatment in 'Melany' was significantly higher than in control.
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