옥수수 유묘에 있어서 L-phenyalanine Ammonia Lyase(PAL)의 추출과 특성

백기엽, 이철희 忠北大學校 1986 論文集 Vol.32 No.-

-본문참고-

춘난 자생지 토양으로 난재배

백기엽,오창호,선정훈,이상선 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1997 연구보고서 Vol.2 No.-

For cultivations of orchid plants, the Korean native orchid plant inhabiting (KNO) soils were collected from the Southern and Western areas in Korea, and employed for understanding the role of symbiotic fungi on the orchids. The parameters related to growth of the orchid plant were investigated after the young nurseries (plantlets) of Cymbidium goeringii, C. kanran, and Neofinetia falcata planted on the sterilized or unsterilized soils of KNO under the conditions of green house. The fresh weights or dried weights of all orchid plants grown on the unsterilized soil for 17 months were highly heavier than those grown on the sterilized soils. Thus, the differences of the plant growth were not found among the four different KNO soils. The various stages of peletons in the root cells of the orchid plants were distinguished from unsterilized soils, while those not quite often in the sterilized soils. Mineral nutrients (K, Ca, Mg, P) related to nitrogen were also measured in the individual plantlets for understanding the plant symbiosis. The nitrogen of the orchid plants absorbed in the unsterilized soils were measured to be comparatively greater than those in sterilized soils during the orchid plant growth. The other minerals of the orchid plants absorbed in the unsterilized soils were also measured to be comparatively greater than those in the sterilized soils. The metabolism of nitrogen were concluded to be much important, and were considered to stimulate the other mineral absorptions in growth of the orchid plants symbiotic with the fungi.

생물반응기를 이용한 국화 기내 배양 시 환경조건이 식물체 증식 및 품질에 미치는 영향

김선자,한은주,백기엽 충북대학교 첨단원예기술개발연구센터 2000 연구보고서 Vol.5 No.-

Salpiglossis sinuata L.의 原形質體 分離 및 培養

李澈熙,韓那榮,白基燁 충북대학교 농업과학기술연구소 1993 農業科學硏究 Vol.10 No.2

In order to attempt a realistic somatic hybridization using S, sinuata, it is imperative that large numbers of viable protoplasts were routinely available. In addition, cultural conditions have to be fully optimized for plant regeneration in order to maximize the probability of recovery, not only of hybrid callus, but of hybrid plants. Therefore, this study was performed to establish the ideal method for this field and results were as followed : Protoplasts were isolated, where possible, from both greenhouse-grown leaf material and cultured cell sources (callus, cell suspension) of wild-type and cytoplasmic albino mutant. The optimum methods for protoplast isolation were successfully established. Generally, cell suspension was the ideal material for protoplast isolation. The method and medium for protoplast culture were also fully optimized with an analysis of their response to a range of protoplast culture media, culture methods (Liquid layer or liquid over agar culture) and initial plating densities. KP8 liquid medium, together with initial plating density of 2.5×104/ml, was most efficient for protoplast culture. The regeneration sequence of protoplast isolation through to whole plants was attempted. Rep-roducible organogenesis was induced by transferring p-calli (5-10mm in diam.) to MSP1, MSD3, MSD4 and MSZ media. The rooting of regenerated wild-type S. sinuata shoots was difficult, but it was still possible to establish plantlets in the greenhouse. Protoplast-derived shoot regenerants of the albino form failed to root.

Ebb and Flood System을 이용한 팔레높시스 양액 재배에서 배양액 농도에 따른 묘 생육 및 양분흡수 패턴

한은주,최영일,백기엽 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-

발광다이오드를 이용한 광질과 일장조절이 '딕시 화이트' 미니 시클라멘의 개화와 생장에 미치는 영향

허정욱,이춘우,백기엽 충북대학교 첨단원예기술개발연구센터 2000 연구보고서 Vol.5 No.-

The study was conducted to investigate the effect of different light qualities and day lengths controlled by using Light-Emitting Diode (LED) of blue, red, or blue plus red provided by LED System on flowering and growth of F1 Cyclamen miniature. Cyclamen persicum 'Dixie White' seedlings which have raised for 170 days after sowing were grown under conditions of 20° C air temperature and 60% relative humidity. Day length was controlled by blue, re, or blue plus red light (1:1 in energy ratio) with 10 or 12 hr for 63 days. Photosynthetic photon flux in the treatments with 10 or 12 hr was about 100 or 83 μmol m-2 s-1, respectively. Fluorescent lamp treatment of 12 hr day length was considered as a control. Flowering and growth in all the treatments were significantly affected by light quality or day length tested in the experiment. Number of flowers, which opened above the leaf canopy, was higher in red light treatment with 10 hr day length than that in control. Days to flowering were the shortest in day length treatment of 10 hr per day with blue plus red light among the treatments. Blooming period was also the longest in red treatment regardless of day length and mixture light treatment, and thus above 20 days prolonged than that in control. Otherwise, number of unfolded leaves or net photosynthetic rate was not significantly affected by the different light qualities and day lengths.

당원, 삼투압 및 무기염 농도가 Panax ginseng 모상근의 생장과 Ginsenosides 생산성에 미치는 영향

한영현,임종훈,백기엽,유기원 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1998 연구보고서 Vol.3 No.-

인삼 형질전환체는 호르몬이 포함되지 않은 3/4SH 배지에 공구유출식 콘형 생물반응기를 이용하여 배양하였다. 초기 접종 량은 30g/3L로 하였으며 4-5일 간격으로 배양액 내의 당원, 전기 전도도와 pH의 변화를 측정하였다. 처음 pH는 5.8이었으나 배양8일 후 4.95까지 떨어졌으나, 이후 5.63까지 증가하였다. 전기전도도와 탄소원으로 제공된 sucrose는 일정하게 감소되었고, 특히 배양 미삼의 탄소원으로 제공된 sucrose는 배양 20일 후에 완전히 소모되었다. 분해산물인 glucose와 frutose는 20일 까지 점진적으로 증가되었고 이후 급격히 감소되었다. 400ml 플라스크 안에 100ml의 배지량을 첨가하고 3g의 배양 미삼을 접종하여 배양하였을 때 배양 미삼의 생장률은 탄소원의 농도에 따라 증가되었다. 이는 배양 미삼의 환원당, 전당, 그리고 전분의 저장함량이 증가되었다. 그러나 ginsenosides의 함량은 반 비례관계로 감소되었다. 배양 주간별 ginsenosides의 함량변화는 배양초기인 1,2 주에는 급격히 감소되었으며 3주에는 약간 증가하고 4,5주 사이에 급격히 증가되었다. 삼투조절제로 사용한 0.1, 0.2, 0.3M의 mannitol과 sobitol은 생장 면에서 0.1M의 실험구가 좋았으나 ginsenosides의 함량은 삼투압농도와 반비례 관계로 급격히 감소되었다. Panax ginseng roots transformed by Agrobacterium rhizogenes were cultured in the air-lifted cone type bubble bioreactor that contained a hormone-free SH medium with 3% sucrose. The inoculating vol. was 30g/3L. The pH, EC, glucose, fructose and sucrose were investigated every 4 or 5 days. The pH was 5.8 initially and become 4.95 after 8 days and then increased after to 5.63. EC and sucrose contents decreased with culture days and then especially sucrose, which is carbon source for bio-ginseng rots, were depleted after 20 days. Fructose and glucose content increased gradually till 20 days with regular intervals, and then decreased quickly. While the roots were culture in 400ml flask, the higher sucrose consents, the higher growth rate. The growth rate and starch, reducing and total sugar contents were increased with the increase of sucrose concenturations but the contents of ginsenoside decrease. Variations of ginsenosides each weeks decreased first 1 or 2 weeks, increase a little after 3 weeks and largely between 4 and 5 weeks of flask culture. The osmotic regulation regents on growth were treated the 0.1, 0.3, 0.5M sobitol and mannitol. Amount of the growth were all increased on 0.1M but largely decreased the ginsenoside.

Ebb & flood 시스템을 이용한 팔레높시스 배양액 개발

최영일,한은주,백기엽 충북대학교 첨단원예기술개발연구센터 2000 연구보고서 Vol.5 No.-

한국 자생란에서 난 균근균의 분리와 유묘난에 접종

오창호,이태수,이상선,백기엽 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1998 연구보고서 Vol.3 No.-

한국의 춘란 자생지의 춘란(Cymbidium goeringii) 뿌리에서 공생하는 10개의 균근균을 분리하였다. 분리된 난 균근균을 PDA에서 배양한 결과 균총은 다양한 색깔을 나타내었으며, 생장 속도도 달랐다. 분리된 난 균근균을 현미경으로 관찰한 결과 Rhizoctonia repens 또는 R. endophytica var endophytica로 동정되었다. R. repens는 자생란인 춘란의 뿌리에서 많이 분리되었으며, R. endophytica var endophytica는 상업적으로 재배되는 외국산의 난 뿌리에서 분리되었다. 분리된 균들 중에 R. repens와 유사한 5개의 분리균을 oatmeal agar에 접종하고 배양한 후 여러종의 난 유묘를 이식하여 난의 생장을 관찰하였다. 그 결과 난의 생장은 분리 균에 따라서 다양하였으나 대부분의 난은 생장촉진 반응을 나타내서, 분리균과 각각의 난과의 공생 관계를 관찰할 수 있었다. Ten isolates of the orchid mycorrhizal fungi were isolated from the roots of Korean native orchid plants (cymbidium goeringii) which inhabitate mainly in southern and western areas of Korea. The growth rates and color of the isolates in potato dextrose agar (PDA) were various. Microscopic observations of the hyphae isolated were identified as Rhizoctonia repens and R. endophytica var endophytica or their related species. R. repens was isolated from the roots of the Korean native orchids, but R. endophytica var endophytica was only isolated from the roots of the commercial orchids introduced from foreign countries. Also, the polymorphic patterns of genomic DNA extracted from selected isolates were compared with those of DNA extracted from the orchid mycorrhizal fungi isolated previously and similar band patterns were observed among those isolates. Five isolates of R. repens were selected and cultured at the oatmeal agar for investigating their symbiosis with orchid plants. The symbiotic specificity between orchid plants and isolated orchid mycorrhizal fungi was observed by growing orchids about six months in the greenhouse. The symbiotic responses of the commercial orchid plants with selected isolates were quite different from different isolates due to the genetic variations.

조팝나무 揷木時期 및 揷床土의 種類가 發根에 미치는 影響

趙鎭泰,尹汰,金泰重,洪承敏,白基燁 한국화훼연구회 1995 화훼연구 Vol.4 No.1

Cutting experiment was conducted to develop a mass propagation method for Spiraea prunifolia var. simpliciflora Nakai. The optimum season for hardwood cutting was between December and early April, but cutting could be done year-round by using rhizome. NAA treatment (100 ppm) was the most effective as rooting promoter. IBA and IAA were also found to be effective. The effect of sand, red clay, loam, silty loam and vermiculite had little differences in rooting especially when cuttings were done earlier than April 5.