間接螢光抗體法 및 免疫黃金標識法을 이용한 肝吸蟲組織內 抗原性部位에 관한 硏究

成大林,金洙鎭,嚴基善,林漢鍾 고려대학교 의과대학 1988 고려대 의대 잡지 Vol.25 No.3

Indirect fluorescent antibody test and immunogold labeling method were accomplished to observe the antigenic localization in the tissues of adult Clonorchis sinesis. Immunoglobulins (IgG) for the reaction were obtained from white rats and rabbits, at eight weeks after experimental infection with 50 and 500 metacercariae of C. sinensis, respectively. Frozen sectioned adult worm antigen (8μm) and FITC-conjugated goat anti-rat, rabbit IgG were used for indirect fluorescent antibody test. On the other hand, sectioned tissue antigen embedded in Lowcryl HM 20 medium and secondary antibody protein A gold complex (particle size:12 nm) were used for immunogold labeling method. In the results, fluorescence or gold particle were not observed on the tissue antigens of the worm when reacted with the noninfected control IgG. However, with the infected animal groups IgG, fluorescence or gold particles were observed on the interstitial matrix (body fluid) between the various organs, such as the tegumental cells, parenchyma, testes, receptaculum seminis. And the cecal epithelium including cecal contents and epithelium of excretory bladder also showed strong reaction. Therefore, antigenic materials which stimulate the IgG antibodies against the host were thought to be fluid substances and digestive enzymes or excretory materials which make up the interstitial matrix in the tissues of the worm.

Drosophila melanogaster Meigen 人工集團內의 두 Allozyme 座位에서의 頻度依存選擇에 관한 硏究

성기창,박미란 성균관대학교 기초과학연구소 1988 論文集 Vol.39 No.1

In D. melanogaster, evidence of frequency-dependent selection has been observed at two loci Adh and α-Gpd. From the both loci the gene frequency changes were carried out between inversion free genotypes at two gene frequency levels (F alleles=20%, 80%). Allele frequency changes were recorded for a period of 11 generations. Genes at both Adh and α-Gpd loci second chromosome were scored by agarose electrophoresis. When homozygote (either FF or SS) was persent in low frequency(20%), its fitness was enhanced, but when the frequency is high(80%, its fitness was reduced. This may idea to a stable polymorism because a generation increases as its frequency decreases. The allelic frequency of Adh approached an equilibrium after 11 generations, converging toward the frequency (case A_1=76%, cage A_2=69%). However, the possibility remains that Adh alleles in nature are associated with alleles at closely linked loci. If linkage disequilbrium is present in the experiments because it exists in nature, then observed effects (frequency-dependent selection) would affect the Adh locus in nature as well. The frequency of α-Gpd approached an equilibrium, converging toward the frequency (cage B_1=40%, cage B_2=33%).

韓國 Drosophila 屬內 16種에 對한 遺傳的 近緣關係

成耆昌,서민철,金源澤,金溶聲 성균관대학교 기초과학연구소 1987 論文集 Vol.38 No.2

Eleven allozymes from sixteen species of Drosophila were analyzed by using horizontal starch gel electrophoresis in order to estimate genetic relationship among these species. All eleven allozymes were present in all sixteen species examined, and some commom alleles were also detected. The mean genetic identity between inter-and intra-subgenus Drosophila were estimated to be 0.16∼0.20, and 0.24∼0.28, respectively. The results showed that D. nigromaculata, which belongs to subgenus Drosophila, had closer genetic relationship to the species in subgenus Sophophora than to Drosophila. High mean genetic identity was also observed between subgenus Hirtodrosophila and Drosophila, and between subgenus Paradrosophila and Sophophora. Results of Nei's genetic identity test of the present data generally agreed with the previously known Drosophila classification establised based on the cytological and morphological characteristics.

Drosophila busckii의 韓國集團에서 있어서 同位酵素 多形現象

成耆昌,尙椿植,金彧,金大鎰 성균관대학교 기초과학연구소 1985 論文集 Vol.36 No.1

Genetic structure and variability were investigated in four Korean populations of Drosophila busckii. Eight allozyme loci were analysed by means of starch gel electrophoresis. The proportions of polymorphic loci and loci heterozygous per individual were estimated to be 0.25 and 0.051, respectively. The average genetic similarity between four populations was found to be S―=0.977. This value is similar to the other Drosophila populations reported. However, the gene variation of D. busckii in this study appeared to be the least variable among 28 Drosophila species reported. It is suggested that the low gene variation in D. busckii might be due to adaptation of this species to a narrow niche, but the possibility of occurrence of the random drift cannot be excluded.

韓國人 指紋型의 遺傳學的 分析

成耆昌,李起俊 성균관대학교 기초과학연구소 1985 論文集 Vol.36 No.2

The finger print pattern of the Korean population has been examined. The predominant finger print type of non-criminal population consisted of 49% Loop type, 45.5% Whorl type, 2.6% Simple arch and 2.5% Tented arch. Among criminal populations, the frequency of occurance of finger print types was found to be 52.2% Loop type, 40.9% Whorl type, 2.5% Tented arch, and 2.8% Simple arch respectively. In addition, criminal population contained 0.3% Accidental type, which was absent in non-criminal population. The total Ridge Number for the non-criminals was 151 for females and 143 for males, while in criminal population it was 127 for females and 138 for males. The atd angle for non-criminal population was 82.29° for males and 83.38° for females. The a-b ridge number was found to be 76.05 for males and 73.24 for females.

Drosophila quinaria Species Group의 遺傳的 近緣關係

成耆昌,金正洙,金大鎰,金彧,金溶聲 성균관대학교 기초과학연구소 1985 論文集 Vol.36 No.2

The genetic relatedness among the Korean Drosophila quinaria species group, including D. nigromaculata, D. unispina, D. brachynephrose, and D. angularis, has been examined by using horizontal starch gel electrophoresis. The results showed that none of the ten isozyme patterns examined for these four species was identical. The average genetic similarity among the four species was found to be 0.440. The similarity among three species. D. unispina, D. brachynephrose, and D. angularis was 0.466. The relatedness was found to be closest between D. nigromaculata and D. unispina, showing 0.640. D. nigromaculata and D. angularis showed relatedness of 0.258. The results suggested that these four species appear to be genetically related at the level of sibling species. The percent polymorphism in D. brachynephrose was found to be 76.9%, showing significant genetic variations.

정상교합자와 골격성 Ⅲ급 부정교합자의 저작운동형태의 비교

성기혁 慶北大學校 齒科大學 1997 慶北齒大論文集 Vol.14 No.-

정상교합자의 골격성 3급 부정교합자의 저작운동형태를 비교연구하기위해서 30명의 정상교합자와 20명의 골격성 3급 부정교합자를 대상으로 츄잉검을 사용하고 Biopak system을 이용하여 전두면상에서 저작운동을 기록, 분석 하였다. 각 개체마다 대표하는 저작경로를 정하여 저작폭경, 개구거리, 개구각, 페구각, 최대개구속도, 최대폐구속도를 알아보았다. 또 저작 경로의 형태에 따라 특징적인 7가지 패턴으로 분류하여 다음의 결과를 얻었다. 1. 정상교합자군에 비해 골격성 3급 부정교합자군에서는 좀 더 다양하고 수직적인 저작양상을 나타내었다. 2. 저작폭경의 비교에서는 골격성 3급 부정교합자군이 정상교합자군에 비해 더 좁게 나타났다(P<0.01). 3. 개구거리에서는 골격성 3급 부정교합자군이 정상교합자군에 비해 더 작게 나타났으나 통계학적으로 유의성은 없었다(P>0.05). 4. 개구각과 폐구각에서는 골격성 3급 부정교합자군에서 정상교합자군보다 더욱 예각으로 나타났다(P<0.01). 5. 최대개구속도, 최대폐구속도에서는 정상교합자군에 비해 골격성 3급 부정교합자군에서 느리게 나타났으나 통계학적으로 유의성은 없었다(P>.05). 6. 저작운동형태에 따른 분류에서 정상교합자군에서 Type 2가 73.4%로 가장 많은 비율을 차지하였으나, 골격성 3급 부정교합자군에서는 Type 3가 35.0%, Type 2가 30.0%로 가장 많은 비율을 차지하였다. 7. 저작운동형태에 따른 분류에서 골격성 3급 부정교합자군에서는 Type 4(chopping type)가 25.0%로 정상교합자군 3.3%에 비해 많은 비율을 차지하였다.

目錄 電算化에 관한 硏究

成耆珠 同德女子大學校 1984 同大論叢 Vol.14 No.1

Computerizing of library system is the main subject in our library circles. There are two different methods in library computerization which one is Total System and the other is Piecemeal System. In general, Piecemeal System is being accepted by system librarians. And the Cataloguing System will be the first step in Piecemeal system. Because the Cataloguing system is the very method to making the Bibliographic Data Base. In this paper two kind of methods concerning the cataloguing system is being represented: 1. Program on Card Printing System 2. Program on Retrieval System. Through the method 1 the various kind of cataloguing cards will be printed out automatically and the retrieval of bibliographic information will be achieved by method 2. Comparing with the manual system, we realize the effectiveness of the computerized cataloguing system.

비디오텍스 시스템과 도서관 봉사간의 관계

成耆珠 한국문헌정보학회 1993 한국문헌정보학회지 Vol.24 No.-

The purpose of this study is to verify the hypothesis that the contents and range of library services are changed as the use of videotex systems increases. 1000 members of Chollian Ⅱ were chosen as subjects for this study and the questionnaire was posted to them. The returned responses to questionnaire were analyzed with the statistical software packages "SASPC" and "STATGRAPHICS". The findings and conclusions of this study are summarised as follows: 1. Users of Chollian Ⅱ make more use of videotex systems in seeking daily information than in seeking scholarly and professional information. It seems desirable to co-ordinate the services of videotex systems and libraries by redefining the traditional boundary of the two systems. Users will then conveniently get daily information through videotex servies, scholarly and professional information through the reference service of libraries. 2. Sex does not make any significant difference in the use of videotex systems. Most frequent use of videotex systems is made by those at the age of thirties. Businessmen and enterprisers make the most frequent use of videotex systems. Those of higher education are the best users. 3. As the use of videotex systems increases, use of newspapers and periodicals decreases while its increase rarely has influence on the use of library materials. 4. There is limited correlation between the use of videotex systems and that of library services. The increase of the use of videotex systems does not significantly increase or decrease the use of libraries. It changes, however, the pattern and methods of information services provided by libraries. 5. Users of special libraries make the most frequent use of videotex systems and there is not much difference in the use by users of other kinds of libraries

사립체 DNA 뉴클레오타이드 3243 A→G 돌연변이 [MTTL1^*MELAS3243G]에 의한 MELAS 증후군

박기형,김승현,이영주,김희태,김주한,김명호,박성섭 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4