Six new dammarane-type triterpene saponins from Panax ginseng flower buds and their cytotoxicity

Ke-Ke Li,Sha-Sha Li,Fei Xu,Xiao-Jie Gong 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.2

Background: Panax ginseng has been used for a variety of medical purposes in eastern countries formore than two thousand years. From the extensive experiences accumulated in its long medication usehistory and the substantial strong evidence in modern research studies, we know that ginseng hasvarious pharmacological activities, such as antitumor, antidiabetic, antioxidant, and cardiovascularsystemeprotective effects. The active chemical constituents of ginseng, ginsenosides, are rich instructural diversity and exhibit a wide range of biological activities. Methods: Ginsenoside constituents from P. ginseng flower buds were isolated and purified by variouschromatographic methods, and their structures were identified by spectroscopic analysis and comparisonwith the reported data. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H- tetrazolium bromidemethod was used to test their cytotoxic effects on three human cancer cell lines. Results: Six ginsenosides, namely 6’emalonyl formyl ginsenoside F1 (1), 3beacetoxyl ginsenoside F1 (2),ginsenoside Rh24 (6), ginsenoside Rh25 (7), 7behydroxyl ginsenoside Rd (8) and ginsenoside Rh26 (10)were isolated and elucidated as new compounds, together with four known compounds (3e5 and 9). Inaddition, the cytotoxicity of these isolated compounds was shown as half inhibitory concentration values,a tentative structureeactivity relationship was also discussed based on the results of our bioassay. Conclusion: The study of chemical constituents was useful for the quality control of P. ginseng flowerbuds. The study on antitumor activities showed that new Compound 1 exhibited moderate cytotoxicactivities against HL-60, MGC80-3 and Hep-G2 with half inhibitory concentration values of 16.74, 29.51and 20.48 mM, respectively.

Recombinant-attenuated Salmonella Pullorum strain expressing the hemagglutinin-neuraminidase protein of Newcastle disease virus (NDV) protects chickens against NDV and Salmonella Pullorum challenge

Ke Ding,Ke Shang,Zu-Hua Yu,Chuan Yu,Yan-Yan Jia,Lei He,Cheng-Shui Liao,Jing Li,Chun-Jie Zhang,Yin-Ju Li,Ting-Cai Wu,Xiang-chao Cheng 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.2

Newcastle disease virus (NDV) and Salmonella Pullorum have significant damaging effects on the poultry industry, but no previous vaccinecan protect poultry effectively. In this study, a recombinant-attenuated S. Pullorum strain secreting the NDV hemagglutinin-neuraminidase(HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed by using the suicide plasmid pREasd-mediated bacteria homologousrecombination method to form a new bivalent vaccine candidate against Newcastle disease (ND) and S. Pullorum disease (PD). The effectof this vaccine candidate was compared with those of the NDV LaSota and C79-13ΔcrpΔasd (pYA) strains. The serum hemagglutinationinhibition antibody titers, serum immunoglobulin G (IgG) antibodies, secretory IgA, and stimulation index in lymphocyte proliferation wereincreased significantly more (p < 0.01) in chickens inoculated with C79-13ΔcrpΔasd (pYA-HN) than with C79-13ΔcrpΔasd (pYA) but werenot significantly increased compared with the chickens immunized with the LaSota live vaccine (p > 0.05). Moreover, the novel strain provides60% and 80% protective efficacy against the NDV virulent strain F48E9 and the S. Pullorum virulent strain C79-13. In summary, in this study,a recombinant-attenuated S. Pullorum strain secreting NDV HN protein was constructed. The generation of the S. Pullorum C79-13ΔcrpΔasd(pYA-HN) strain provides a foundation for the development of an effective living-vector double vaccine against ND and PD.

Expression of ERCC1, MSH2 and PARP1 in Non-small Cell Lung Cancer and Prognostic Value in Patients Treated with Platinum-based Chemotherapy

Xie, Ke-Jie,He, Hong-Er,Sun, Ai-Jing,Liu, Xi-Bo,Sun, Li-Ping,Dong, Xue-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Purpose: To evaluate the prognostic value of the expression of excision repair cross-complementation group l (ERCC1), MutS protein homolog 2 (MSH2) and poly ADP-ribose polymerase 1 (PARP1) in non-small-cell lung cancer patients receiving platinum-based postoperative adjuvant chemotherapy. Methods: Immunohistochemistry was applied to detect the expression of ERCC1, MSH2 and PARP1 in 111 cases of non-small cell lung cancer paraffin embedded surgical specimens. Through og-rank survival analysis, we evaluated the prognostic value of the ERCC1, MSH2, PARP1 and the related clinicopathological factors. COX regression analysis was used to determine whether ERCC1, MSH2 and PARP1 were independent prognostic factors. Results: In the enrolled 111 non-small cell lung cancer patients, the positive expression rate of ERCC1, MSH2 and RARP1 was 33.3%, 36.9% and 55.9%, respectively. ERCC1 (P<0.001) and PARP1 (P=0.033) were found to be correlated with the survival time while there was no correlation for MSH2 (P=0.298). Patients with both ERCC1 and PARP1 negative cancer had significantly longer survival time than those with ERCC1 (P=0.042) or PARP1 (P=0.027) positive alone. Similalry, the survival time of patients with both ERCC1 and PARP1 positive cancer was shorter than those with ERCC1 (P=0.048) or PARP1 (P=0.01) positive alone. Conclusion: Patients with ERCC1 or PARP1 negative non-small cell lung cancer appear to benefit from platinum-based postoperative adjuvant chemotherapy.

Improved Fiber Uniformity and Jet Number in Multi-spinneret Electrospinning via Auxiliary Electrode

Yu-Ke Wu,Zong-Jie Li,Jie Fan,Liang Wang,Peng-Fei Zhang,Jian Liu,Yong Liu 한국섬유공학회 2019 Fibers and polymers Vol.20 No.6

Auxiliary-electrode electrospinning process is a novel technique to improve nanofiber production rate Due to theformation of multiple jets. In the present work, dual-spinneret (DSE) and tri-spinneret electrospinning (TSE) with anauxiliary electrode were employed to evaluate the number of jets and fiber uniformity in this electrospinning process. Theresults showed that all the spinnerets in DSE and TSE with an auxiliary electrode could produce 2-12 jets, which could beadjusted by controlling the process parameters such as applied voltage and injection speed. The number of jets initiallyincreased and then decreased with a rise in applied voltage in these two systems. Moreover, the produced nanofibers weresmaller in size and possessed a more uniform fiber diameter per distribution in comparison to those without the auxiliaryelectrode. This work provides a new insight on mass production of nanofibers and designing electrospinning apparatus.

Six new dammarane-type triterpene saponins from Panax ginseng flower buds and their cytotoxicity

Li, Ke-Ke,Li, Sha-Sha,Xu, Fei,Gong, Xiao-Jie The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.2

Background: Panax ginseng has been used for a variety of medical purposes in eastern countries for more than two thousand years. From the extensive experiences accumulated in its long medication use history and the substantial strong evidence in modern research studies, we know that ginseng has various pharmacological activities, such as antitumor, antidiabetic, antioxidant, and cardiovascular system-protective effects. The active chemical constituents of ginseng, ginsenosides, are rich in structural diversity and exhibit a wide range of biological activities. Methods: Ginsenoside constituents from P. ginseng flower buds were isolated and purified by various chromatographic methods, and their structures were identified by spectroscopic analysis and comparison with the reported data. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H- tetrazolium bromide method was used to test their cytotoxic effects on three human cancer cell lines. Results: Six ginsenosides, namely 6'-malonyl formyl ginsenoside F₁ (1), 3β-acetoxyl ginsenoside F₁ (2), ginsenoside Rh₂₄ (6), ginsenoside Rh₂₅ (7), 7β-hydroxyl ginsenoside Rd (8) and ginsenoside Rh₂₆ (10) were isolated and elucidated as new compounds, together with four known compounds (3-5 and 9). In addition, the cytotoxicity of these isolated compounds was shown as half inhibitory concentration values, a tentative structure-activity relationship was also discussed based on the results of our bioassay. Conclusion: The study of chemical constituents was useful for the quality control of P. ginseng flower buds. The study on antitumor activities showed that new Compound 1 exhibited moderate cytotoxic activities against HL-60, MGC80-3 and Hep-G2 with half inhibitory concentration values of 16.74, 29.51 and 20.48 μM, respectively.

Buckling Analysis of Columns Ended by Rotation-stiffness Spring Hinges

Ke Cao,Yao-Jie Guo,Jun Xu 한국강구조학회 2016 International Journal of Steel Structures Vol.16 No.1

Stabilities of compressed columns ended by spring hinges with particular rotation stiffness was a basic theory for more complex structures’ researches and engineering, however investigations on calculation approaches for these columns were rarely reported. This paper focus on buckling analysis on these spring hinge ended columns, and the derivation of the columns’ effective length factor equation were presented. Meanwhile a practical design formula to predicate the effective length factors was proposed and further validated to be highly accurate. Furthermore impacts of ideal-ends column method, which ignoring real ends’ rotation stiffness, were investigated. Finally conditions making ideal-ends column method accurate enough in practice were put forward and the practical condition equations were proposed.

DIESEL-LIKE FUEL PRODUCTION FROM CATALYTIC CRACKING AND ESTERIFICATION OF WASTE OIL

Jie CHEN,Jian Chun JIANG,Xiao An NIE,Jun Ming XU,Xia CHANG,Ke LI 한국신재생에너지학회 2012 AFORE Vol.2012 No.-

The Antifungal Mechanism of Electrolyzed Oxidizing Water against Aspergillus flavus

Ke Xiong,Xiu-ting Li,Sunbao Guo,Li-te Li,Hai-jie Liu 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.2

Fungicidal electrolyzed oxidizing waters (EOW)include neutralized electrolyzed oxidizing water (NEW)and acidic electrolyzed oxidizing water (AcEW). Theseforms were evaluated against Aspergillus flavus conidia(spores) and mycelia. EOW possessed strong antifungalactivities against A. flavus conidia and mycelia due todestruction of the cellular structures of the A. flavusconidium and mycelium. K+ and Mg2+ leakage results fromdamage to the normal cellular functions of A. flavus conidiaand mycelia. Among the physicochemical parameters ofEOW, the available chlorine concentration (ACC) isprimarily responsible for the EOW antifungal activity. A. flavus can be eliminated by use of AcEW and NEW as anew, alternative method of fungal control that is superior tosome current physical methods and to synthetic chemicalfungicides.

Domain Switching and Crack Propagation of BaTiO3 Single Crystal in Different Environments

( Ke Wei Gao ),( Xian Wu Zhao ),( Rui Min Wang ),( Li Jie Qiao ),( Wu Yang Chu ) 한국부식방식학회(구 한국부식학회) 2008 Corrosion Science and Technology Vol.7 No.6

Novel Phage Display-Derived H5N1-Specific scFvs with Potential Use in Rapid Avian Flu Diagnosis

( Jie Wu ),( Xian Qiao Zeng ),( Hong Bin Zhang ),( Han Zhong Ni ),( Lei Pei ),( Li Rong Zou ),( Li Jun Liang ),( Xin Zhang ),( Jin Yan Lin ),( Chang Wen Ke ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.5

The highly pathogenic avian influenza A (HPAI) viruses of the H5N1 subtype infect poultry and have also been spreading to humans. Although new antiviral drugs and vaccinations can be effective, rapid detection would be more efficient to control the outbreak of infections. In this study, a phage-display library was applied to select antibody fragments for HPAI strain A/Hubei/1/2010. As a result, three clones were selected and sequenced. A hemagglutinin inhibition assay of the three scFvs revealed that none exhibited hemagglutination inhibition activity towards the H5N1 virus, yet they showed a higher binding affinity for several HPAI H5N1 strains compared with other influenza viruses. An ELISA confirmed that the HA protein was the target of the scFvs, and the results of a protein structure simulation showed that all the selected scFvs bound to the HA2 subunit of the HA protein. In conclusion, the three selected scFVs could be useful for developing a specific detection tool for the surveillance of HPAI epidemic strains.