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Self-Assessment and Working time on Nut Size Distinction Skill of Marine Engineers
KIKUCHI Kazumitsu 제어로봇시스템학회 2022 제어로봇시스템학회 국제학술대회 논문집 Vol.2022 No.11
A rule of thumb for marine engineers is that if a marine engineer has much experience in maintaining machines, the marine engineer can distinguish the size of the tool (spanner or wrench) required for removing machine nuts or bolts by mere observation [1]. This study investigates whether this rule of thumb is related to self-assessment and working time for the distinction of nuts. The participants in this experiment were marine engineering students without practical experience. The experiment included visual and hand conditions. The experiment place was not only a ship engine room but a training room where noise, high temperature, and the smell of fuel oil in the marine engine plant were not experienced by the students. The results showed that the number of the nuts accurately distinguished was unaffected by working hours and self-assessment of the participants who had a boarding history of 12 months on the training ship. Safe operation on marine engine plants must rely on the skills of marine engineers, such as a nut size distinction skill.
Cryopreservation and low-temperature storage of seeds of Phaius tankervilleae
Tomonari Hirano,Toshinari Godo,Kazumitsu Miyoshi,Keiko Ishikawa,Masaya Ishikawa,Masahiro Mii 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.1
In this study we established reliable methods for conservation of seeds of Phaius tankervilleae as an orchid genetic resource. The seeds, which were dehydrated to 5% water content and preserved at 4C, showed no decrease in viability and germinability after three months. After storage for six months, however, the seeds showed a drastic decrease in germinability, even though survival rate was high. For long-term preservation of seeds of P. tankervilleae, cryopreservation is applied to the freshly harvested seeds. When the seeds were cryopreserved by the vitrification method for up to 12 months there was no apparent deterioration effect of storage time. These results indicate that cryopreservation by the vitrification method is useful for long-term conservation of P. tankervilleae seeds, which are difficult to preserve for more than three months under dry and low-temperature conditions. In this study we established reliable methods for conservation of seeds of Phaius tankervilleae as an orchid genetic resource. The seeds, which were dehydrated to 5% water content and preserved at 4C, showed no decrease in viability and germinability after three months. After storage for six months, however, the seeds showed a drastic decrease in germinability, even though survival rate was high. For long-term preservation of seeds of P. tankervilleae, cryopreservation is applied to the freshly harvested seeds. When the seeds were cryopreserved by the vitrification method for up to 12 months there was no apparent deterioration effect of storage time. These results indicate that cryopreservation by the vitrification method is useful for long-term conservation of P. tankervilleae seeds, which are difficult to preserve for more than three months under dry and low-temperature conditions.
Ogawa, Atsushi,Taguchi, Nanako,Miyoshi, Kazumitsu The Korean Society of Plant Biotechnology 2010 Plant biotechnology reports Vol.4 No.2
We used a flow cytometer to investigate the variations in endopolyploidy (the frequencies of nuclei with DNA contents equivalent to 4C through 16C) during the short period of the early growing stage in vigorously growing young tissues of maize seedlings. We examined different portions of the root and leaves that had been growing for 7 (day 7) and 13 (day 13) days after germination. Endoreplication showed two opposing phenomena without aging. In one case, the endopolyploidy of the first leaf was higher on day 13 than on day 7. In the latter case, endopolyploidy decreased, as clearly revealed by a comparison of the endopolyploidy of the second leaves and the 160-170 mm portion of the seminal root on days 7 and 13. Endopolyploidy was also lower in the top of the leaf. In roots, endopolyploidy was increased by the exogenous application of abscisic acid for only 1 day. The levels of endopolyploidy increased without an increase in cell size in the roots. These results showed that endoreplication occurs in actively growing and young tissue and that the variation can be induced in the short period examined.
Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol
Liu, Shu-Lin,Sheng, Ren,Jung, Jae Hun,Wang, Li,Stec, Ewa,O'Connor, Matthew J,Song, Seohyoen,Bikkavilli, Rama Kamesh,Winn, Robert A,Lee, Daesung,Baek, Kwanghee,Ueda, Kazumitsu,Levitan, Irena,Kim, Kwang NATURE PUBLISHING GROUP 2017 NATURE CHEMICAL BIOLOGY Vol. No.
<P>Controlled distribution of lipids across various cell membranes is crucial for cell homeostasis and regulation. We developed an imaging method that allows simultaneous in situ quantification of cholesterol in two leaflets of the plasma membrane (PM) using tunable orthogonal cholesterol sensors. Our imaging revealed marked transbilayer asymmetry of PM cholesterol (TAPMC) in various mammalian cells, with the concentration in the inner leaflet (IPM) being -12-fold lower than that in the outer leaflet (OPM). The asymmetry was maintained by active transport of cholesterol from IPM to OPM and its chemical retention at OPM. Furthermore, the increase in the IPM cholesterol level was triggered in a stimulus-specific manner, allowing cholesterol to serve as a signaling lipid. We found excellent correlation between the IPM cholesterol level and cellular Wnt signaling activity, suggesting that TAPMC and stimulus-induced PM cholesterol redistribution are crucial for tight regulation of cellular processes under physiological conditions.</P>