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Chen, Bin,Kayukawa, Takumi,Monteiro, Antonia,Ishikawa, Yukio Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6
The cytosolic members of the HSP70 family of proteins play key roles in the molecular chaperone machinery of the cell. In the study we cloned and sequenced the full-length cDNA of Delia antiqua HSP70 gene, which is 2461 bp long and encodes 643 a.a. with a calculated molecular mass of 70,787 Da. We investigated gene copies of cytosolic HSP70 members of 4 insect species with complete genome available, and found that they are quite variable with species. In order to characterize this protein we carried out an alignment and a phylogenetic analysis with 41 complete protein sequences from insects. The analysis divided the cytosolic members of the family into two classes, HSP70 and HSC70, distinguishable on the basis of 15 residues. HSP70 class members were slightly shorter in length and smaller in molecular mass relative to the HSC70 class members, and the conservative and functional regions in these sequences were documented. Mainly, we investigated the expression of Delia antiqua HSP70 gene, in response to diapauses and thermal stresses. Both summer and winter diapauses elevated HSP70 transcript levels. Cold-stress led to increased HSP70 expression levels in summer- and winter-diapausing pupae, but heat-stress elevated the levels only in the winter-diapausing pupae. In all cases, the expression levels, after being elevated, gradually decreased with time. HSP70 expression was low in non-diapausing pupae but was up-regulated following cold- and heat-stresses. Heat-stress gradually increased the mRNA level with time whereas cold-stress gradually decreased levels after an initial increase.
Kenji Shimomura,Hinoki Oikawa,Takumi Kayukawa,Satoshi Asamizu,Nobuhiro Suzuki,Shunsuke Yajima,Motohiro Tomizawa 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3
Plant-derived compounds such as essential oils (EOs) are sources of protection for stored products. In some cases, inactive ingredients that co-occur with EOs may render them more toxic or less desirable. Thus, there has been a shift away from extract-based crude mixtures to single repellent substances. The repellency activity of the capsaicin agonist, vanillyl butyl ether (VBE), which is a thermal-sense compound affecting the transient receptor potential vanilloid (TRPV) 1 channel of mammals, was investigated for control of the red flour beetle, Tribolium castaneum. The repellency activity of VBE was dose-dependent and almost comparative to DEET in an area preference test. To clarify the mode of action, RNA interference (RNAi) was used for transcriptional knockdown of two TRPV channels, Nanchung (TcNan) or Inactive (TcIav). Reduction of each of the transcript levels led to a significant decrease in VBE repellency activity, but no significant decrease in DEET repellency. These results suggested that the repellency activity of VBE was mediated by the TRPV channels, and capsaicin agonists might serve as an alternative to EOs for the protection of stored products.
Marumo Yoshiaki,Yoshida Takashi,Furukawa Yuki,Ina Kenji,Kamiya Ayumi,Kataoka Takae,Kayukawa Satoshi 대한백신학회 2023 Clinical and Experimental Vaccine Research Vol.12 No.4
Purpose: Patients with hematological malignancies are at an increased risk of severe infection with coronavirus disease 2019 (COVID-19). However, developing an adequate immune response after vaccination is difficult, especially in patients with lymphoid neoplasms. Since the long-term effects of the BNT162b2 vaccine are unclear, the humoral immune response 5 months after the two vaccinations in patients with hematological disorders was analyzed. Materials and Methods: Samples were collected from 96 patients vaccinated twice with BNT162b2 and treated with at least one line of an antitumor or immunosuppressive drug in our hospital from November 2021 to February 2022. Serum anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) spike (S) antibody titers were analyzed. Patients were age- and sex-matched using propensity matching and compared with a healthy control group. Patients with serum anti-SARS-CoV-2 S antibodies were defined as ‘responder’ if >50 U/mL. The patients had B-cell non-Hodgkin lymphoma (B-NHL), multiple myeloma, chronic myeloid leukemia, etc. Results: Patients had significantly low antibody levels (median, 55.3 U/mL vs. 809.8 U/mL; p<0.001) and a significantly low response rate (p<0.001). Multivariate analysis showed that patients with B-NHL, aged >72 years, were associated with a low response to vaccination. There were no significant differences between patients with chronic myeloid leukemia and healthy controls. Conclusion: Our study shows that patients with hematological disorders are at risk of developing severe COVID-19 infections because of low responsiveness to vaccination. Moreover, the rate of antibody positivity differed between the disease groups. Further studies are warranted to determine an appropriate preventive method for these patients, especially those with B-NHL.