A Synthetic Naringenin Derivative, 5-Hydroxy-7,4′-diacetyloxyflavanone-<i>N</i>-phenyl Hydrazone (N101-43), Induces Apoptosis through Up-regulation of Fas/FasL Expression and Inhibition of PI3K/Akt Signaling Pathways in Non-Small-Cell Lung Cancer Cells

Bak, Yesol,Kim, Heejong,Kang, Jeong-Woo,Lee, Dong Hun,Kim, Man Sub,Park, Yun Sun,Kim, Jung-Hee,Jung, Kang-Yeoun,Lim, Yoongho,Hong, Jintae,Yoon, Do-Young American Chemical Society 2011 Journal of agricultural and food chemistry Vol.59 No.18

<P>Naringenin, a well-known naturally occurring flavonone, demonstrates cytotoxicity in a variety of human cancer cell lines; its inhibitory effects on tumor growth have spurred interest in its therapeutic application. In this study, naringenin was derivatized to produce more effective small-molecule inhibitors of cancer cell proliferation, and the anticancer effects of its derivative, 5-hydroxy-7,4′-diacetyloxyflavanone-<I>N</I>-phenyl hydrazone (N101-43), in non-small-cell lung cancer (NSCLC) cell lines NCI-H460, A549, and NCI-H1299 were investigated. Naringenin itself possesses no cytotoxicity against lung cancer cells. In contrast, N101-43 inhibits proliferation of both NCI-H460 and A549 cell lines; this capacity is lost in p53-lacking NCI-H1299 cells. N101-43 induces apoptosis via sub-G<SUB>1</SUB> cell-cycle arrest in NCI-H460 and via G<SUB>0</SUB>/G<SUB>1</SUB> arrest in A549 cells. Expression of apoptosis and cell-cycle regulatory factors is altered: Cyclins A and D1 and phospho-pRb are down-regulated, but expression of CDK inhibitors such as p21, p27, and p53 is enhanced by N101-43 treatment; N101-43 also increases expression levels of the extrinsic death receptor Fas and its binding partner FasL. Furthermore, N101-43 treatment diminishes levels of cell survival factors such as PI3K and p-Akt dose-dependently, and N101-43 additionally induces cleavage of the pro-apoptotic factors caspase-3, caspase-8, and poly ADP-ribose polymerase (PARP). Cumulatively, these investigations show that the naringenin derivative N101-43 induces apoptosis via up-regulation of Fas/FasL expression, activation of caspase cascades, and inhibition of PI3K/Akt survival signaling pathways in NCI-H460 and A549 cells. In conclusion, these data indicate that N101-43 may have potential as an anticancer agent in NSCLC.</P>

A sub-1-volt nanoelectromechanical switching device.

Lee, Jeong Oen,Song, Yong-Ha,Kim, Min-Wu,Kang, Min-Ho,Oh, Jae-Sub,Yang, Hyun-Ho,Yoon, Jun-Bo Nature Pub. Group 2013 Nature nanotechnology Vol.8 No.1

<P>Nanoelectromechanical (NEM) switches have received widespread attention as promising candidates in the drive to surmount the physical limitations currently faced by complementary metal oxide semiconductor technology. The NEM switch has demonstrated superior characteristics including quasi-zero leakage behaviour, excellent density capability and operation in harsh environments. However, an unacceptably high operating voltage (4-20 V) has posed a major obstacle in the practical use of the NEM switch in low-power integrated circuits. To utilize the NEM switch widely as a core device component in ultralow power applications, the operation voltage needs to be reduced to 1 V or below. However, sub-1 V actuation has not yet been demonstrated because of fabrication difficulties and irreversible switching failure caused by surface adhesion. Here, we report the sub-1 V operation of a NEM switch through the introduction of a novel pipe clip device structure and an effective air gap fabrication technique. This achievement is primarily attributed to the incorporation of a 4-nm-thick air gap, which is the smallest reported so far for a NEM switch generated using a 'top-down' approach. Our structure and process can potentially be utilized in various nanogap-related applications, including NEM switch-based ultralow-power integrated circuits, NEM resonators, nanogap electrodes for scientific research and sensors.</P>

2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242) induces apoptosis via modulating E7 expression and inhibition of PI3K/Akt pathway in SiHa human cervical cancer cells.

Kim, Man Sub,Kim, Jung Hee,Bak, Yesol,Park, Yun Sun,Lee, Dong Hun,Kang, Jeong Woo,Shim, Jung-Hyun,Jeong, Heon Sang,Hong, Jin Tae,Yoon, Do Young Lawrence Erlbaum Associates, Publishers [etc.] 2012 Nutrition and cancer Vol.64 No.8

<P>The Maillard reaction is a chemical reaction occurring between an amino acid and a reducing sugar, usually requiring thermal processing. Maillard reaction products (MRPs) have antioxidant, antimutagenic, and antibacterial effects, and although 2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242), a fructose-tyrosine MRP, appears to inhibit proliferation of cancer cells, its mechanism of action has not been studied in detail. We found that HPB242 treatment modulated expression of cyclins and tumor suppressor genes in SiHa human cervical cancer cell lines: cyclins and phospho-pRB were downregulated, whereas the expression of CDK inhibitors and p53 was enhanced. HPB242 induced apoptosis dose-dependently by suppressing E7 expression and leading to sub-G1 cell-cycle arrest in SiHa cell lines; treatment also led to the proteolytic cleavage of caspase-3, -9, and poly (ADP-ribose) polymerase. Moreover, HPB242 upregulated Fas expression, altered expressions of pro- and antiapoptotic factors, and also inhibited nuclear translocation of nuclear factor κB and phosphorylation of IκB. HPB242 treatment decreased phosphatidyl inositol-3 kinase and p-Akt expression levels, demonstrating that this survival pathway may also be inhibited by HPB242. Cumulatively, HPB242 promotes apoptosis by influencing E7 expression, inducing cell-cycle arrest at sub-G1 phase, and promoting both intrinsic (mitochondrial) and extrinsic (Fas-dependent) apoptosis in SiHa human cervical cancer cells.</P>

전자식 선박디젤엔진의 엔진제어기 개발/연구

심한섭(Han-Sub Sim),이민광(Min-Kwang Lee),이강윤(Kang-Yoon Lee) 한국기계가공학회 2015 한국기계가공학회지 Vol.14 No.5

A control program of an engine control module (ECM) was developed, and its control performance was verified on a 750Ps marine diesel engine. The control method was designed for an engine rotational speed control system. For ECM hardware, the commercial rapid control prototype (RCP) ECM was used. The programming tool for control algorithm development was the MatLab/Simulink. The main control algorithm assembled many control models as engine cranking, run, and stall. Each model has sub-models to input/output control signals. The target engine speed was input signal from a speed control lever, and control output signal of the ECM was sent to the unit-injectors for fuel injection. The engine test was performed under various conditions of engine rotational speeds and dynamometer loads. The test results show that the control function of the ECM is suitable for electrical marine diesel engines.

신이식에서 이식신의 생존분석

오선미,김종학,황평주,구영선,강민규,나기량,김종섭,김성숙,이강욱,신영태,설종구,배진선,손기섭 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

To investigate the prognostic factors for the survival of transplanted kidney in patients with end-stage renal failure, 59 cases of renal transplantation from September 1986 to Feburary 1997 in Chungnam National University Hospital were analyzed retrospectively. The results were as follows: 1)The mean age of recipients was 33.8 years and that of doners was 38.9 years. The male to female ratio of recipients was 2.5:1, and that of donors was 1.03:1 2) Living related donore(LRD) were 79.6% and living non-related donors were 20.3%. The HLA-identical donors(ID) in LRD were 18.7% and HLA-haploidentical donors(HID) were 61.0%. In living non-related donors(LNRD), mean matched HLA-AB antigens were 1.56 and mean matched HLA-DR antigens were 0.56. 3) The average 5-year patient survival fate was 94%, and average 5-year graft survival rate was 70%. The 5-year graft survival fate of HLA-ID was 100%, and those HLA-HID and LNR were 70% and 36% respectively. 4) Total 33 episodes of acute rejection were found in 45.8% of transplanted patients. The number of acute rejection episode did not show significant difference between LRD and LNRD(P > 0.05). There was no significant difference in MLC between two groups(p > 0.05). 5) In comparison between the 18 patients who lost their graft function in 5 years and 17 patients who are maintaining graft function for more than 5 years, MLC was significantly lower in patients with functioning graft than of patients with non-functioning graft (p < 0.05). The number of rejection episode was also lower in patients with functioning graft than that of the patients with non-functioning graft(p < 0.05). However, there were no significant difference in recepient and donor age and history of pre-transplantation donor specific transfusion between two groups. With the results above, we can speculate that adequate donor selection according to good matched HLA typing and low MLC is very important for graft survival in renal transplantation. Prevention and treatment of acute rejection in renal transplantation is also na important factor for graft survival.

High-throughput screening of protein variations in soybean seeds.

Vijayanand Velusamy,Kyung Jun Lee,Bo-Keun Ha,Jin Baek Kim,Sang Hoon Kim,Si-Yong Kang,Dong Sub Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The protein in soybean seeds accounts for approximately 40% of the dry seed weight. Two major storage proteins, 7S and 11S, constitute 70-80% of the total storage proteins in the seeds. In this study, the variation of total soluble protein extracts from 1152 soybean landraces that have been collected from South Korea were studied using high-throughput screening method with HT Protein Express Labchip (Caliper Life Sciences, Inc.). Seven distinct protein band patterns - four protein sub-units of 11S and three sub-units of 7S, were taken into account and their presence or absence were analyzed. Among the 1152 landraces, 525 genotypes were identified as lacking lipoxygenase, 255 lacking α1 subunit, 680 lacking α subunit, 169 lacking β subunit, 140 lacking acidic, 114 lacking Kunitz Trypsin Inhibitor (KTi) and 199 lacking basic protein patterns. The high-throughput protein analysis is helpful in screening a large number of populations with less time and minimum labor. The selected genotypes with low amounts or lacking of anti-nutritional factors such as trypsin inhibitor, lipoxygenase and α subunit would be used for future breeding purpose of quality improvement in soybean protein.

칼라코에 줄기切片의 器官分化에 미치는 生長調節物質의 影響

李康燮,呂邑東,蘇雄永 全北大學校 基礎科學硏究所 1991 基礎科學 Vol.14 No.1

In order to elucidate the effects of growth regulators on organogenesis in the stem explants of Kalanchoe daigremontiana, we have cultured the explants for 10 weeks on the MS medium with or without ammonium nitrate, supplemented with simultaneous combinations of auxin(IAA), gibberellin(GA_3) and cytokinin(BAP)The cocentrations of these growth regulators were 0.001-10.0 ㎎/l, respectively. The callus formation and organogenesis in the explants are mainly achieved under the simultaneous actions of the auxin and cytokinin. The optimal combination of growth regulators for callus formation was IAA 10.0 ㎎/l, GA_3 0.001 ㎎/l and BAP 0.1 ㎎/l. The optimal combinations for the adventitious root and bud formation are IAA 10.0 ㎎/l, GA_3 0.01 ㎎/l, BAP 0.01 ㎎/l and IAA 0.1 ㎎/l, GA_3 0.01 ㎎/l, BAP 1.0 ㎎/l, respectively. On the MS medium without ammonium nitrate, the callus and adventitious bud formation in the explants are better than those on the MS medium with ammonium nitrate, but the adventitious root formation is worse. The correlation between callus formation and organogenesis is negative on the both media.

도시근교 농촌지역 공동주택 농가의 내부공간이용 행태 특성에 관한 연구

김강섭,김석규,이상정 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(계획계)

Apartments in rural areas were built without consideration of their surroundings. The subject of study is a farmhouse that agriculture is the main occupation amongst the residents of apartments. This study aims to examine the space usage patterns of interior space within these apartments. We observed the design of the interiors followed a typically urban pattern. This does not fit a farmhouse lifestyle. Residents have felt uncomfortable because they do not have secure storage in their apartments. Therefore this study concludes the design of apartments in rural areas should be reconsidered to accommodate the needs of rural residents.

ZnO 투명전도막의 전기적 특성에 미치는 Al₂O₃의 도핑 농도의 효과

박강일,김병섭,이성욱,김현수,이세종,곽동주 경성대학교 공학기술연구소 2003 공학기술연구지 Vol.10 No.-

Al doped Zinc Oxide(ZnO:AI) films, which is widely used as a transparent conductor in optoelectronic devices such as solar cell, liquid crystal display, plasma display panel, thermal heater, and other sensors, were prepared by using the capacitively coupled DC magnetron sputtering method. In this paper the effect of doping amounts of Al₂0₃ on the electrical, optical and morphological properties were investigated experimentally. The results show that the structural and electrical properties of the film are highly affected by the doping. The optimum growth conditions were obtained for films doped with 2 wt% of indium which exhibit a resistivity of 8.5×10^(-4)Ω-cm associated with a transmittance of 91.7 % for 840 nm in film thickness in the wavelength range of the visible spectrum.

中風急性期에 活用되는 鎭肝熄風湯에 관한 臨床的 考察

沈廷燮,金剛山 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.1

최근 식생활의 변화와 노년층의 증가 등으로 中風이 증가하고 있어 동서의학적인 연구가 활발히 이루어지고 있다. 中風은 本虛表實證으로 인식되고 主風說, 主氣說, 主火說, 主痰濕說 등이 있으나 葉天士가 內風說을 개창한 이래 肝腸上亢, 肝腎陰虛 등에 의한 肝風內動이 주병기로 인식되고 있으며 內風은 양기가 음혈의 억제를 받지 못하여 과항하는 것이라 할 수 있다. 따라서 張錫純의 鎭肝熄風湯이 본 肝風內動證에 많이 사용되고 있으며 본 병원에서도 임상에 사용되고 있는 바 구체적인 치료효과에 대해 알아보고자 본 연구를 시행하였다. We studied the meaning and use of the Jingansickphungtang, so we found the Jingansickphungtang is effective of preventing early cerebral stroke. We had prescirbed the abstract of the Jingansickphungtang in 24 early cerebral stroke patients for six days from May, 1997 to December, 1997 and the results was as follows. 1. We observed recovering rates after a week; headache 72.7%, dizziness 75%, chest discomfort 83.3%, red face 93.7%, yawning sign 100%; average recovering rates 84.1%. 2. We observed the states of consciousness after a week. There were increasing from 20 patients to 23 patients in Gr.1 and decreasing from 4 patients to 1 patient in Gr.2. 3. We observed the states of swallowing after a week. There were increasing from 16 patients to 19 patients in having general diet and decreasing from 6 patients to 5 patients in having soft diet and decreasing from 2 patients to 0 patient in having liquid diet. 4. We observed the states of aphasia after a week. There were incresing from 7 patients to 16 patients in Gr.5 and decreasing from 13 patients to 4 patients in Gr.4. 5. We observed the states of motor weakness after a week. Upper limbdegeneration; 0 patient, changelessness; 13 patients(54.1%), improvement; 11 patients(45.9%) Lower limbdegeneration; 0 patient, changelessness; 12 patients(50%), improvement; 12 patients(50%) As the results, the Jingansickphungtang have effects in preventing of the most serious neurological symptomsmental disorder, dysphasia, aphagia and hemiparesis in early cerebral stroke.