당뇨병 환자에서 비타민 E 영양제 보충이 체내 지질과산화 및 혈액 지방성분에 미치는 영향연구

강남이,민경완 서울보건대학 1999 서울보건대학 부설 병원경영연구소 논문집 Vol.4 No.2

We investigated the effects of vitamin E supplementation on lipid peroxidation and on the activities of antioxidative enzymes in diabetes mellitus. Vitamin E supplement doses consist of the amout 400 I.U./day (d-α-tocopherol. 268.5mg). After vitamin E supplementation. plasma levels of total cholesterol and LDL-cholesterol were decreased. and increased in plasma triglyceride. HDL-cholesterol. Plasma levels of lipid peroxide measured as thiobarbituric acid reactive substances(TBARs) before and after vitamin E supplementation was 0.271. 0.181 nmol/100ml plasma. respectively. Plasma level of TBARs. indicator of lipid peroxidation and increased susceptability of LDL towards lipid peroxidation. were elevated in diabetes before vitamin E supplementation. while the plasma level of TBARs was significantly decrease after vitamin E supplementation. indicating that decreased lipid peroxidation are associated with the antioxidative activities effects of vitamin E. In the present study the possible explanations for that diabetes have a higher risk of diabetic complications by cardiovascular disease include the changes of blood lipid and lipoprotein concentration, and vitamin E supplementation which might have protective functions against free radical against free radical-mediated lipid peroxidation.

NMR study of hydrogen exchange during the B-Z transition of a DNA duplex induced by the Zα domains of yatapoxvirus E3L

Lee, E.H.,Seo, Y.J.,Ahn, H.C.,Kang, Y.M.,Kim, H.E.,Lee, Y.M.,Choi, B.S.,Lee, J.H. North-Holland Pub ; Elsevier Science Ltd 2010 FEBS letters Vol.584 No.21

The Yaba-like disease viruses (YLDV) are members of the Yatapoxvirus family and have double-stranded DNA genomes. The E3L protein, which is essential for pathogenesis in the vaccinia virus, consists of two domains: an N-terminal Z-DNA binding domain and a C-terminal RNA binding domain. The crystal structure of the E3L orthologue of YLDV (yabZα<SUB>E3L</SUB>) bound to Z-DNA revealed that the overall structure of yabZα<SUB>E3L</SUB> and its interaction with Z-DNA are very similar to those of hZα<SUB>ADAR1</SUB>. Here we have performed NMR hydrogen exchange experiments on the complexes between yabZα<SUB>E3L</SUB> and d(CGCGCG)<SUB>2</SUB> with a variety of protein-to-DNA molar ratios. This study revealed that yabZα<SUB>E3L</SUB> could efficiently change the B-form helix of the d(CGCGCG)<SUB>2</SUB> to left-handed Z-DNA via the active-mono B-Z transition pathway like hZα<SUB>ADAR1</SUB>1.

Identification of antigenic Edwardsiella tarda surface proteins and their role in pathogenesis

Yu, J.E.,Yoo, A.Y.,Choi, K.H.,Cha, J.,Kwak, I.,Kang, H.Y. Academic Press 2013 Fish & shellfish immunology Vol.34 No.2

Edwardsiella tarda causes an infectious fish disease called edwardsiellosis. Several outer membrane proteins (OMPs) are associated with virulence factors and are attractive as vaccine candidates. In this study, 4 immuno-reactive OMPs of E. tarda were detected using anti-sera from flounder infected with E. tarda. Using matrix-assisted laser desorption/ionization mass spectrometry analyses, 2 of the 4 OMPs were identified as OmpA and murein lipoprotein (Lpp), which are highly conserved surface proteins in gram-negative bacteria. For further characterization of these surface proteins, we generated ompA- and lpp-inactivated mutants by insertion of a kanamycin cassette in the corresponding genes, and named these mutants E. tarda CK99 and CK164, respectively. As expected, immuno-reactive OmpA and Lpp proteins were absent in E. tarda CK99 and CK164, respectively, confirming that OmpA and Lpp are antigenic surface proteins. Interestingly, the LD<SUB>50</SUB> value of E. tarda CK164 in fish (2.0 x 10<SUP>8</SUP> colony-forming unit [CFU]/fish) was greater than that of the parental strain (3.0 x 10<SUP>7</SUP> CFU/fish). The LD<SUB>50</SUB> of E. tarda CK99 did not differ from that of its parental strain. After administering attenuated E. tarda CK164 to fish, we monitored the E. tarda-specific immune response profile. We observed that the E. tarda-specific serum IgM titer increased in a time-dependent manner, and was much higher than the value observed after the administration of a heat-killed E. tarda control. Moreover, fish vaccinated with E. tarda CK164 were 100% protected when challenged by CK41, a pathogenic strain. Our results suggest that E. tarda CK164 can potentially be used for developing an effective live attenuated vaccine for edwardsiellosis that can be applied in the aquaculture industry.

Characterization of Interface States in MOS Systems by Using Photonic High-Frequency Capacitance-Voltage Responses

S.J.Song,H.T.Kim,S.S.Chi,M.S.Kim,W.S.Chang,S.D.Cho,H.T.Shin,T.E.Kim,H.J.Kang,D.J.Kim,D.M.Kim 한국물리학회 2002 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.41 No.6

Based on the photonic high-frequency capacitance-voltage response of Metal-Oxide-Semiconductor capacitors, we report an improved characterization method for the analyzing of interface states in MOS systems. An optical source with a photonic energy $E_{ph}$ = 0.943 eV ($\lambda$ = 1314.5 nm) is employed for photonic deep-depletion (fast sweep rate) high-frequency Capacitance-Voltage (photonic DD HF-CV) characterization of interface states distributed in the photo-responsive energy band. Using the photonic DD HF-CV characterization, we obtained a U-shaped distribution of $D_{it}$ over ($E_V + E_g/2 - q\phi_f) < E_t < (E_V + E_g/2 - q\phi_f + q\phi_S$) for N-type Metal-Oxide-Semiconductor capacitors and ($E_C - E_g/2 - q\phi_f + q\phi_S) < E_t < (E_C - E_g/2 - q\phi_f$) for P-type Metal-Oxide-Semiconductor capacitors.

Molecular investigation of tick-borne pathogens in ticks from grazing cattle in Korea

Kang, S.W.,Doan, H.T.T.,Choe, S.E.,Noh, J.H.,Yoo, M.S.,Reddy, K.E.,Kim, Y.H.,Kweon, C.H.,Jung, S.C.,Chang, K.Y. Elsevier 2013 Parasitology international Vol.62 No.3

This study was carried out to identify the tick species that infest grazing cattle and to determine the presence of tick-borne pathogens transmitted by these ticks in Korea. A total of 903 ticks (categorized into 566 tick pools) were collected from five provinces during 2010-2011. The most prevalent tick species was Haemaphysalis longicornis, followed by three Ixodes spp. ticks. The collected ticks were infected with both rickettsial and protozoan pathogens. In all, 469 (82.9%) tick pools tested positive for the Anaplasma/Ehrlichia 16S rRNA gene, whereas 67 (11.8%) were positive for the Babesia/Theileria 18S rRNA gene. Among the rickettsial pathogens, E. canis was detected with the highest rate (22.3%), followed by A. platys (20%), E. chaffeensis (19.4%), E. ewingii (19.3%), Rickettsia sp. (12.4%), A. phagocytophilum (5.5%) and E. muris (0.5%). Among the protozoan pathogens, T. equi was detected with the highest rate (7.2%), followed by T. sergenti/T. buffeli (3.7%) and B. caballi (0.35%). Simultaneous infections with up to seven pathogens were also identified. In particular, ticks infected with rickettsial pathogens were also infected with protozoan pathogens (22 samples). All five provinces investigated infected with tick-borne pathogens.

A Computer Simulation of the Four Charm Production and Fragmentation in e+e- Collisions

E. Won,Daekyoung Kang,H.C. Ha,J.H. Choi 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.48 No.II

Theoretical predictions of the rate of exclusive production of two charmonia in e+e. annihilation differ significantly from measurements by the Belle collaboration. Up to now, there has been no conclusive explanation for this substantial discrepancy. However, recent theoretical works have suggested that it may be possible to identify the origin of the discrepancy from a study of four charm inclusive events. We present necessary computational steps required for such an experimental study at B factories. Event generation of the reaction e+e. ! c.cc.c and the fragmentation of charm quarks are simulated. These two important computational steps provide all the information necessary to measure the total cross section of the four charm hadron final state at B factories.

Prevalence and characteristics of intimin-producing Escherichia coli strains isolated from healthy chickens in Korea.

Oh, J-Y,Kang, M-S,An, B-K,Shin, E-G,Kim, M-J,Kim, Y-J,Kwon, Y-K Poultry Science Association, etc 2012 Poultry science Vol.91 No.10

<P>Virulent Escherichia coli strains have commonly been associated with diarrheal illness in humans and animals. Typical enteropathogenic Escherichia coli (EPEC) with intimin gene (eaeA) and E. coli adherence factor plasmid, or atypical EPEC with only eaeA have been implicated in human cases. In the present study, we investigated the prevalence of virulence-associated genes including eaeA in the E. coli strains isolated from cloacal specimens of 184 chicken flocks in 7 provinces in Korea between 2009 and 2010. When 7 virulence genes (VT1, VT2, LT, and ST for enterotoxigenic E. coli; eaeA and bfpA for enteropathogenic E. coli; and aggR for enteroaggregative E. coli) were screened by multiplex PCR, a total of 30 E. coli strains carrying only the eaeA gene were detected from 184 flocks that were identified as atypical enteropathogenic Escherichia coli (aEPEC). The aEPEC strains were analyzed by eae subtyping, phylogenetic grouping PCR, and serotyping. Twelve (40%) of 30 aEPEC strains possessed an eae-관 subtype, followed by 관 (30%), 관 (16.7%), and 관1 (13.3%). Eight (26.7%) of 30 aEPEC strains were designated into the phylogenetic group A. Two (6.7%) and 3 (10%) aEPEC strains were classified into the phylogenetic group B2 and D, respectively. A total of 15 (50%) aEPEC strains were serotyped to groups O24, O25, O26, O71, O80, O103, and O157, and the remaining strains were nontypeable. In analyzing the genetic diversity among the 30 aEPEC isolates by the pulsed-field gel electrophoresis method with XbaI-digestion, the pulsed-field gel electrophoresis profiling produced 20 different patterns, but isolates within the same group did not show clear geographic or breed relationships. Our data indicate that healthy chickens may constitute an important natural reservoir of aEPEC strains, and suggest that transmission to humans could not be excluded.</P>

Prediction of Growth of Escherichia coli O157 : H7 in Lettuce Treated with Alkaline Electrolyzed Water at Different Temperatures

Tian Ding,Yong-Guo Jin,S. M. E. Rahman,Jai-Moung Kim,Kang-Hyun Choi,Gye-Sun Choi,오덕환 한국식품위생안전성학회 2009 한국식품위생안전성학회지 Vol.24 No.3

This study was conducted to develop a model for describing the effect of storage temperature (4, 10, 15, 20, 25, 30 and 35oC) on the growth of Escherichia coli O157 : H7 in ready-to-eat (RTE) lettuce treated with or without (control) alkaline electrolyzed water (AIEW). The growth curves were well fitted with the Gompertz equation, which was used to determine the specific growth rate (SGR) and lag time (LT) of E. coli O157 : H7 (R2 = 0.994). Results showed that the obtained SGR and LT were dependent on the storage temperature. The growth rate increased with increasing temperature from 4 to 35oC. The square root models were used to evaluate the effect of storage temperature on the growth of E. coli O157 : H7 in lettuce samples treated without or with AIEW. The coefficient of determination (R2), adjusted determination coefficient (R2 Adj), and mean square error (MSE) were employed to validate the established models. It showed that R2 and R2 Adj were close to 1 (> 0.93), and MSE calculated from models of untreated and treated lettuce were 0.031 and 0.025, respectively. The results demonstrated that the overall predictions of the growth of E. coli O157 : H7 agreed with the observed data. This study was conducted to develop a model for describing the effect of storage temperature (4, 10, 15, 20, 25, 30 and 35oC) on the growth of Escherichia coli O157 : H7 in ready-to-eat (RTE) lettuce treated with or without (control) alkaline electrolyzed water (AIEW). The growth curves were well fitted with the Gompertz equation, which was used to determine the specific growth rate (SGR) and lag time (LT) of E. coli O157 : H7 (R2 = 0.994). Results showed that the obtained SGR and LT were dependent on the storage temperature. The growth rate increased with increasing temperature from 4 to 35oC. The square root models were used to evaluate the effect of storage temperature on the growth of E. coli O157 : H7 in lettuce samples treated without or with AIEW. The coefficient of determination (R2), adjusted determination coefficient (R2 Adj), and mean square error (MSE) were employed to validate the established models. It showed that R2 and R2 Adj were close to 1 (> 0.93), and MSE calculated from models of untreated and treated lettuce were 0.031 and 0.025, respectively. The results demonstrated that the overall predictions of the growth of E. coli O157 : H7 agreed with the observed data.

Effects of estrogen and estrogenic compounds, 4-tert-octylphenol, and bisphenol A on the uterine contraction and contraction-associated proteins in rats

An, B.S.,Ahn, H.J.,Kang, H.S.,Jung, E.M.,Yang, H.,Hong, E.J.,Jeung, E.B. North-Holland 2013 Molecular and cellular endocrinology Vol.375 No.1

We examined the effects of estradiol (E2), 4-tert-octylphenol (OP), and bisphenol A (BPA) on uterine contractions in immature rats. The expression and localization of contraction-associated proteins (CAPs), and contractility of rat uterus with a collagen gel contraction assay were analyzed. E2, OP, and BPA all increased oxytocin (OT)-related pathway, while the prostaglandin-related signaling was reduced. Interestingly, E2 and estrogenic compounds showed distinct effects on the contractile activity of uterine cells. E2 enhanced the contractility, while OP and BPA significantly decreased it. Immunohistochemical analysis of CAPs showed distinct regulation of prostaglandin F receptor localization by E2 and estrogenic compounds, which may explain the different contractile activities of those reagents. In summary, we demonstrate that E2, OP, and BPA regulate CAP expression in a similar manner in the immature rat uterus, however, the effects on contractile activity were modulated differently. These findings suggest that OP and BPA interfere with uterine contractility.

웨이퍼 렌즈를 이용한 소형전자빔 리소장치 개발

김진석(J.S. Kim),김한수(H.S. Kim),이동윤(D.Y. Lee),이석우(S.W. Lee),강은구(E.G. Kang) 한국생산제조학회 2011 한국생산제조시스템학회 학술발표대회 논문집 Vol.2011 No.4

In recently, trends of the miniaturization of micro parts machining pattern by laser machining methods have been emerged. However, the conventional laser processing methods have limitations on the resolution. So, e-beam process has been considered as alternatives. Especially, e-beam lithography has been taken accounted of tools for micro and nano pattern. But e-beam lithography equipment have some disadvantages such as lower productivity and smaller processing area etc. than other micro parts machining tools. Therefore we have carried out the development of micro column electron beam lithography equipment for achieving the more higher productivity, lager processing area, and also higher resolution. And then we tried to develop e-beam lithography system with micro column type and using wafer lens for multi column e-beam lithography system and modular e-beam lithography unit. In this paper, we developed the electro static lens system, composed of accelerator lens, condenser lens, objective lens, beam blanker, stigmator and deflector.