Application of in vitro ovary culture for cottonwood (Populus deltoides) breeding

Kang,Ho-Duck,Lee,Min-Soon 한국자원식물학회 2004 Plant Resources Vol.7 No.2

Five different poplar hybrids were tested for rescuing embryo to elongate in vitro plantiets after hybridization. Ovaries and ovules were cultured on Woody Plant Medium (WPM) supplemented with cytokinins, 6-benzylamine (BA) and zeatin. Multiple shoots were initiated from half section of capsule with immature embryos after 21 days from pollination and tiny shoots were formed after the expansion of cotyledons in ovule cultures. Germinating response was better in intraspecific hybrids (6.53±1.66) than interspecific crosses (0.93±0.54) 수식 이미지 from half section of capsules on WPM medium. In general, zeatin was better than BA in inducing multiple shoots from isolated ovules. The highest average number (19.40±4.53) of shoots was produced from immature ovules of 21 days post-pollination of WPM medium supplemented with 5.0 mg/L zeatin. The highest percentage of germination was 93% from the half section of in vitro ovary cultures. Soil acclimation was successfully conducted in cell tray containing artificially mixed soil with 96% survival rate.

A New Sesame Variety, “Kangbaek” with Disease Resistance, Lodging Tolerence and High Yield

Kang-Bo Shim,Churl-Whan Kang,Suk-Bok Pae,Si-Kyu Lim,Yu-Young Lee,Duck-Yong Suh,Jae-Whan Rho,Jin Song,Dong-Whi Kim,Ho-Young Kim 한국육종학회 2005 한국육종학회지 Vol.37 No.5

A new sesame variety Kangbaek was developed from Yeongnam Agricultural Research Institute in 2004. Cros wasmade by lodging resistant SP9003-3 line to the F1with high yielding capacity, folowed by pedigree selection, yield test and RYT by the sesame breeding team at the National Insti-tute of Crop Science up to 2003. The variety showed higher disease resistance, especially phytophothra disease, and lodging resis-respectively. It's 1000 grains weight was about 2.54 g indicating 0.03 g heavier than that of Yangbaekkae, and it's oil content wasabout 52%. Kangbaek also contained total 7.65mg/g of such lignans as sesamin and sesamolin. The average yield of Kang-baek was 82 kg per 10a at the national-wide regional performance.

야쿠트어 폐쇄자음 연구-러시아어 차용어휘 분석-

강덕수 ( Duck Soo Kang ),김지선 ( Jee Sun Kim ),홍준희 ( Jun Hee Hong ) 한국외국어대학교 언어연구소 2007 언어와 언어학 Vol.0 No.39

Duck-soo Kang.Jee-sun Kim.Jun-hee Hong. 2007. A Study of Obstruents in Yakut through Analysis of Borrowed Words from Russian. Language and Linguistics 39, 1-16. One of the most important constraints in Yakut is connected with syllabic structure. Its syllabic structure is based on CV. So the constraint of "NoGeminates" is strictly applied. And the harmony between features is also considered important. Yakut has the strict constraint on the phonotactical relations. In Yakut a feature [constricted glottis] works more vigorously than [voiced]. This feature is combined with a feature [+back] or [+lab], making new constraints on the initial place: *#[+back, -CG], *# [+lab,+CG]. Finally, consonantal assimilation in obstruents of Yakut is dominated by 2 lows of constraints. VowelHarmony >> Ident-CV[pl] >> NCP >> *[+palatal]# >> Ident-IO; *#[+back, -cont][+back,-high] >> *#[+back,-CG], *# [+lab,+CG] >> [+cont] >> NoGem >> Ident-IO >> *[+voiced]#

In vitro shoot proliferation of Alnus japonica (Thunberg) Steudel

Kang,Ho-duck,Lee,Min-Soon 한국자원식물학회 2004 Plant Resources Vol.7 No.1

In vitro proliferation system was achieved by using nodal segment excised from greenhouse grown juvenile stock plants of Alnus japonica. Stem explants were cultured on MS medium supplemented with different plant growth regulators of cytokinin and/or their combinations. The most effective cytokinin source was the combination of zeatin 2.0 mg/L and TDZ 0.05 mg/L producing the average number of shoots (16.8±3.6). In addition, healthy roots were formed after small clumps of shoots were transferred to half strength of MS medium containing IBA 0.02 mg/L with optimal rooting capacity. Soil acclimatization was successfully conducted in cell tray containing artificially mixed soil with 92 % survival rate.

쑥의 翰燥方法에 따른 脂肪酸 變化에 관하여

김덕웅(Duck-Woong Kim),최강주(Kang-Ju Choi) 한국식품영양과학회 1985 한국식품영양과학회지 Vol.14 No.2

생쑥의 건조방법에 따른 지방산 변화를 검토하기 위하여 강화도산 생쑥을 陽翰, 陰翰, 熱風陽燥 및 冷凍翰燥시켜 ethyl ether extract함량과 그 구성 지방산의 함량 변화를 조사하였다.<br/> 1. Ethyl ether extract의 함량은 冷凍翰燥된 쑥이 5.68%로 가장 높았고 熱風翰燥된 쑥이 2.45%로 가장 낮았다.<br/> 2. GLC에 의한 지방산 분석 결과 11종의 지방산 중 주된 지방산은 필수 지방산인 linoleic acid와 linolenic acid외에 palmitic acid가 다량 함유되어 식품 영양학적인 면에서 고무적인 것으로 사료된다.<br/> 3. 건조 방법에 따른 건조쑥의 지방산의 종류에는 차이가 없었으나 그 조성은 상이하였다. 즉 冷凍翰燥된 쑥은 酸化되기 쉬운 高度 不飽和脂肪酸(C_(18:2)+C_(18:3))의 함량이 높을 뿐만아니라 低沸點脂肪酸들의 감소가 가장 적었다. 한편 熱風翰燥된 쑥은 低沸點脂肪酸들이 상당량 감소되었으나 高度 不飽和脂肪酸은 陰翰 및 陽翰시킨 쑥보다 다소 안정하였으며 陽翰시킨 쑥의 변화가 가장 현저하였다. This study was carried out to investigate changes in the compositions of fatty acids according to the different drying methods of raw mugwort. Raw mugwort from Kang-wha was dried with four methods such as sundrying, shady sun-drying, heated oven-drying and freeze-drying. Total contents and fatty acid compositions of the ethyl ether extracts from the dried mugworts were examined. Total contents of the ethyl ether extracts showed remarkable differences with drying methods; freeze-dried mugwort had the highest content (5.60%), while oven dried mugwort revealed the lowest content (2.45%). Eleven fatty acids and four unkown peaks were identified by gas-liguid chromatography; major fatty acids were linoleic acid, linolenic acid and palmitic acid. Fatty acids of mugworts dried by 4 different methods were the same in kinds, but different in their compositions. The content of polyunsaturated fatty acids (C_(18:2)+C_(18:3)) was the highest and the loss of low volatile fatty acids was the least in mugwort dried by the freeze-drying method. The volatile fatty acids considerably decreased in oven-dried mugwort. However, the contents of polyunsaturated fatty acids were a little higher in ovendried mugwort than in sun-dried and shady sun-dried mugwort, and the lowest in sun-dried mugwort.

Stevioside의 浸出方法의 改善 및 糖質甘味의 正味性에 對한 硏究

姜參龍,李德秀 명지대학교 1982 明大論文集 Vol.13 No.-

現在 多量으로 생산 보급되고 있는 인공감미료의 결점을 보완하기 위한 硏究가 활발히 진행되고 있는데 이의 대체 방안의 하나로써 天然甘味料의 개발이 그 中의 하나이다. 감미료는 식품뿐만 아니라 의약품에서도 그 重要性이 날로 증대되어 가고 있는 실정이다. 본 연구에서는 現在 先進國에서 硏究되고 있는 天然甘味料인 Stevioside의 浸出 方法 및 浸出된 Stevioside의 甘味를 구미에 맞게 改善하여 食品 및 의약용 첨가제로서 널리 활용해 보고자 하는데 그 目的을 두고 있다. 본 硏究를 위한 實驗 結果를 요약하면 다음과 같다. 즉 stevioside의 浸出에 적당한 溫度는 60℃ 이었으며 浸出時間은 6時間 정도가 가장 좋았다. 또 여기에서 얻은 stevioside의 甘味는 우수하였으나, Glucose나 fructose등과 같은 단당류를 혼용했을 경우에도 보다 더 우수하였다. 또 pH 안정도 실험에서는 stevioside 용액은 중성 및 산성에서 보다 안정하였으나 알카리성에서는 불안정하였다. 以上의 結果로 부터 stevioside는 人工甘味料의 대체 甘味料로서 최적일 것으로 생각되나 수요에 충족하기 위해서는 재배 및 생산기술에 대한 보다 더 깊은 연구가 요청된다. The time has come that the development of natural sweetening materials is urgent. Especially the development of stevioside is beginning to show signs of activity in the developed countries. In these days, the appearance of natural sweetening materials as food additives for health and hygiene becomes necessary. The aims of this study are the experiment of the past study, leaching and manufacturing the materials depending on our actual circumstances, and producing the most excellent additives. The result of this study are as follows; 1. The appropriate temperature for the leaching of stevioside was 60℃ and the time needed for the leaching was about 6 hours. 2. In spice of the mixed sweetening materials of stevioside, maltose was better than glucose. 3. The stevioside of the sweetening effect was larger than sugar.

삼각피판법과 밀라드씨법을 겸한 술식에 의한 선천 성구순열교정의 치험예

강동훈,전건수,김덕래 大韓成形外科學會 1987 Archives of Plastic Surgery Vol.14 No.1

There are several well established methods of primary cleftlip repair. Nowadays, traingular flap method and rotation advancement flap method are most widely used and regarded as a method of choice for the repair of cleft lip. However, in cases with severe vertical height difference between cleft side and non cleft side, both methods have some problems. With the Millard`s method for the repair in these cases, the incision of rotation flap must be extended to the philtral column on the noncleft side. This induces a break in the upper part of philtrum and it is not always easy to achieve smooth continuity of the Cupid`s bow. With the triangular flap method for the repair in these cases, large traingular flap has to be designed for lengthening. It produces fairly noticeable scar on the philtrum and divides the philtrum into two parts. Onizuka reported a method in which a small triangular flap was added together with that of Millard in 1966. Y, Nishimura modified Onizuka method in 1978. We performed a method in which triangular flap was put on the end of vermilion ridge with Millard`s method for the repair of cleft lip in cases which vertical hight differences are severe, usually more than 5mm in length in the infant. The result of this method is proved to be very successful and eliminates or reduces the problems previously described. ―Advantages of this method― 1. Permit lengthening even in severe forms without making large z-plasty scar in the philtrum. 2. Preserve philtral dimple and column better than triangular flap method. 3. Natural appearence of the upper part of philtrum. 4. Better correction of nasal deformties than triangular flap method. 5. Good muscle anatomic realignment. 6. In the Second stage operation of bilateral cleft lip, expected lengthening can be achieved without making protrusion of the lower margin of the prolabium.

Efficacy of Intracytoplasmic Sperm Injection for Leukocytospermia

강희규,--,--,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

White blood cells (WBCs) are present in most human ejaculates, but abnormally high concentration of seminal leukocytes map reflect an underlying pathological condition. The World Health Organization (WHO) has defined leukocytospermia as status of more than 10^(6) WBC/mL of semen. The purpose of this study was firstly, to compare the outcomes between conventional IVF and ICSl in leukocytospermia, and secondly, to investigate whether ICSI may be an alternation treatment for patients with leukocytospermia. Total 346 cycles of conventional IVF and ICSI candidates underwent IVF cycles at Eulji Hospital Infertility Clinic. Semen Parameters including concentration, motility, morphology of spermatozoa and concentration of leukocytes were assessed from the raw ejaculates. There was no difference in sperm concentration, motility and morphology. The rates of fertilization and good embryo development from ICSI were significantly higher than those from conventional IVF in leukocytospermia (60.4% & 32.5% respectively for ICSI group and 44.4% & 28.5%, respectively for IVF group, P<0.001). The pregnancy rate after ICSI was also higher than that from conventional IVF (34.0% vs 29.1%. P<0.05). These results indicate that the presence of seminal leukocyes (>1×10^(6) WBC/mL of semen) is adversely related with fertilization, embryo development and pregnancy rate. Therfore the measurement of seminal leukocytes in routine semen analysis appears to be of prognostic value with regard to male fertilizing potential. In conclusion. it is suggested that ICSI is an alternative choice of treatment for patients with leukocytospermia.

McCoy 세포에서 Chlamydia trachomatis의 병원성 인자에 관한 연구

강정숙,최지영,차영주,김영주,김덕례,여명구,박열 조선대학교 생명과학연구소 1998 생명과학 연구 Vol.6 No.-

본 연구는 병원성 세균인 Chlamydia trachomatis(C. trachomatis)가 숙주세포에침습시 C. trachomatis의 병원성 인자와 C. trachomatis와 상호작용하는 숙주 세포막의 인자를 규명함으로써 숙주세포에 대한 감염율을 향상시켜 보다 향상된 진단 방법을 개발하기 위하여 수행하였다. C. trachomatis로 감염된 세포에서 기본소체의 막 단백질을 분리한 결과 각 혈청형의 39 kDa과 42 kDa에서 major outer membranes(MOMPs)이 관찰되었으며, 혈청형 E와 G의 MOMPs는 42.5 kDa으로 동일하였지만 혈청형 F, H, I, 그리고 K의 MOMPs는 각각 다르게 관찰되었다. C. trachomatis가 숙주세포로 침습시 관련된 병원성 인자에 대하여 조사하고자 단층세포로 배양된 배양용기에 C. trachomatis를 24, 48 그리고 73시간대별로 접종시켜 관찰한 결과 72시간의 MOMPs의 양이 가장 많이 증가하였으며 이 결과로 MOMPS이 C. trachomatis 병원성에 중요한 작용을 함을 확인할 수 있었다. C. trachomatis가 침습시 숙주세포의 막 단백질 변화양상을 관찰하기 위해 정상세포와 C. trachomatis로 1, 2, 3 그리고 4시간대별로 접종 시간을 달리한 숙주세포의 막 단백질을 분리한 결과 42 kDa의 단백질 양이 Chlamydia에 감염된 세포 내에서 증가됨을 관찰할 수 있었다. 이로 42 kDa의 막 단백질이 침습시 숙주세포 표면에 대한 수용체로서 작용함을 알 수 있었다. The present study was performed to analyze the pathogenic factors involved in the invasion process of Chlamydia trachomatis(C. trachomatis) into McCoy cells. In this study, elementary bodies(EBs) of C. trachomatis serotypes(E, F, G, H, I, K and LGV) could be isolated by the method of Percoll density gradient centrifugation and the pathogens of serotypes were also compared from whole-cell lysates by using sodium dodecyl sulfate-polyacrylamide gel elelectrophoresis. All chlamydial serotypes showed a single predominant protein ranging from 39 to 42 kDa. The major outer membrane proteins(MOMPs) of the E and G serotypes had an identical molecular weight of 42.5 kDa. In contrast, the MOMPs of the F, H, I and K serotypes were showed different molecular weights of MOMPs. By immunoblotting with anti-MOMP antibody it was revealed that the quantify of MOMPs was time-dependently increased when McCoy cells were infected with Chlamydia for 24, 48 and 72 hr. These results indicated that MOMPs are closely related to chlamydial infection into the host cell.

레이저 박피술의 피부면적 감소효과와 레이저 punch out 병변의 치유에 대한 실험적 연구

강동희,윤을식,구상환,안덕선,박승하 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

An experimental study was designed to analyze the surface reduction effect of ultrapulse carbon dioxide laser on the skin of guinea pigs receiving laser resurfacing. Two squares of skin measuring 3x3 ㎠ were designed on each side of the abdomen of guinea pigs(n=10). Five animals received laser resurfacing on their abdomen at energy of 250mJ/5 watt, and five other animals received 500 mJ/5 watt. On each animal, the left side square was treated with one pass of a CO2 laser, and the right side q=square was treated with two passes using a C.P.G. scanner. The size of the resurfacing area was determined by celluloid overlays at 1,2,3,4 and 6 weeks. Immediately following laser resurfacing, the area was decreased to 69%(left side) and 56%(right side) of the designed area in the 250 mJ/5 watt group, and 59% and 40% in the 500mJ/5 watt group. After 6 weeks, the resurfaced area had been reduced to 70% and 56% of the designed area in the 250mJ/5 watt group, and 60% and 40% in the 500mJ/5 watt group. As a result, the effect of surface reduction by CO2 laser resurfacing increased at a higher power and when more passes of the ultrapulse carbon dioxide laser were applied. The laser treated skin continued to maintain its contracted dimensions. These findings suggest that ultrapulse carbon dioxide laser may be an effective treatment method for rhytides. Another experiment was performed on multiple punch-out lesions on guinea pig skin(n=2) with 1,2,3 mm collimated hand pieces for the purpose of determining a new laser treatment method. After 4 weeks, on gross and histological examination, there were no differences in the healing of multiple punch-out lesions and the healing of punch-out lesions was complete without any scarring. These findings suggest that the laser punch-out method can be used for the treatment of acne scars.