동맥혈 채혈후 시간 경과 및 온도 변화가 가스분압 및 PH 에 미치는 영향에 관한 연구

김동수,이승환,김건식,강화자,신광일,여민구 대한마취과학회 1989 Korean Journal of Anesthesiology Vol.22 No.6

Blood gas samples are highly susceptible to preanalytic error due to improper methods of obtaining or handling the sample prior to delivery to the laboratory. The errors in the measurement of blood gas analysis are currently derived from the exposure of sample to atmosphere, effects of anticoagulant itself, temperature difference between the measuring electrode and drawn blood and the delay in running the sample. To study the effects of the delay in measuring the sample and the temperature difference between the measuring electrode and drawn blood on values of blood gases and pH, we analyzed the arterial sampling from the 24 patients who were taking elective surgery or on his/her recovery period with indwelling arterial catheter. The plastic sampling syringes were kept at 4。C (refrigerator) or 22。-24。C (room temperature) and analyzed at regular intervals (1, 10, 30, 60,120 min) for 120 minutes. The following results were obtained: 1) When the arterial blood drawn from the anesthetized patients were stored 4。C, partial pressure of oxygen (PaO₂) decreased significantly after 20 min, whereas those stored at room temperature decreased significantly after 10 min. 2) When the arterial blood drawn from the recovery patients were stored at 4。C, PaO₂ did not decrease significantly through the experimental period of 120 min. Although those stored at room temperature did not decrease significantly through the period of 120 min. 3) Partial pressure of carbon dioxide in the arterial blood (PaCO₂,) drawn from the anesthetized patients increased significantly by 120 min. at 4。C, whereas those at room temperature increased significantly after 20 min. 4) PaCO₂, of the recovery patients increased signigicantly by 120 min. at 4。C, whereas those at room temperature increased significantly after 30 min. 5) pH of the arterial blood drawn from either anesthetized or recovery patients decreased significantly by 120 min. at 4。C, whereas those at room temperature decreased significantly after 60 min. 6) No significant changes al oxygen saturation (SaO₂) and content (CaO₂) were noted in either anesthetized or recovery. patients in accordance with time elapsed at 4。C or room temperature. In summary, as the changes of PO₂ in particular higher than physiologic PO₂ and PCO₂ in the arterial blood stored at room temperature are significant in accordance with the delay in measuring, it would be advisable to analyze the sample in a short period of time or to store it in a cool place when the measuring will be delayed.

과열증기처리 반탄화 추출물의 항산화 효과 검증에 관한 연구

오근혜(Geun Hye Oh),남정빈(Jeong Bin Nam),양승민(Seung Min Yang),정원희(Won Hee Joung),정진산(Jin San Jeong),신종민(Jong Min Shin),강석구(Seog Goo Kang) 한국펄프·종이공학회 2018 펄프.종이기술 Vol.50 No.2

The antioxidant properties of the extracts from torrefied wood subjected to superheated steam treatment were investigated. Total polyphenolics, total flavonoids, DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity, reducing power, and FRAP (Ferric reducing antioxidant power) were determined to evaluate antioxidant activity. The total polyphenol content was 879.67±40.41 ㎎/mL at a concentration of 25 g/mL when the extract was subjected to a temperature of 300℃ for 10 min, and 759.67±25.17 ㎎/mL at a concentration of 25 g/mL at 350℃ for 10 min. The value of at 300℃ was 15.79% higher than that obtained at 350℃. Total flavonoid content was 111.18±3.55 ㎎/mL at a concentration of 25 g/mL at 300℃ for 10 min, and 80.58±2.58 ㎎/mL at a concentration of 25 g/mL at 350℃ for 10 min. The value at 300℃ was 37.97% higher than that obtained at 350℃. For the DPPH free radical scavenging activity, the highest scavenging activity was observed at a concentration of 6 g/mL. DPPH free radical scavenging activity at a concentration of 6 g/mL at 300℃ for 10 min was 89.83±0.03%, whereas at 350℃ for 10 min at the same concentration, it was found to be 87.99±0.1%. The value at 300℃ was 2.09% higher than that obtained at 350℃. Reducing power was determined to be 3.59±0.04 at a concentration of 25 g/mL at 300℃ for 10 min, and 2.92±0.1 at a concentration of 25 g/mL at 350℃ for 10 min. The value at 300℃ was 22.94% higher than that obtained at 350℃. FRAP was 1742±37 μM at a concentration of 25 g/mL at 300℃ for 10 min, and 1106±8 μM at a at 350℃ for 10 min. At 300℃, the FRAP value was 57.50% higher than that obtained at 350℃. Based on these results, we suggest that torrefied wood treated with superheated steam can be used in various applications because of its effective antioxidant properties.

레미콘 슬러지를 이용한 불소제거

강민구(Min Koo Kang),신관우(Gwan Woo Shin),이상일(Sang Ill Lee) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.11

본 연구의 목적은 칼슘염을 함유한 레미콘 슬러지를 이용하여 불소함유폐수를 물에 난용성인 CaF₂ 화합물 형태로 침전시켜 처리하는데 있어 pH, 레미콘슬러지 주입량, Seed물질 주입량, 교반속도, 교반시간의 최적 조건을 도출하고자 하는데 있다. 실험결과 레미콘 슬러지를 이용한 대상폐수의 CaF₂ 침전 반응에서 함수율 및 불소의 제거효율 등을 고려할 경우 최적의 pH 6, 레미콘 슬러지 주입량은 10 g/L, Seed 물질 주입량은 2 g/L, 교반속도은 100 rpm, 교반시간은 60 min으로 관찰되었다. 이 때 Seed 물질의 주입은 불소제거효율에는 영향이 없는 것으로 나타났지만 침전물의 형성이 되는 반응을 촉진시키고 플럭형성을 원활하게 하여 고액 분리가 잘 되어 함수율이 낮아지는 결과가 나타났다. The purpose of this study was performed to investigate the optimum conditions of pH, concrete sludge, seed dosage, mixing intensity, operation time in treating fluoride-containing wastewater as CaF₂ using the ready-mixed concrete sludge. Considering fluoride removal, water content, that pH 6, concrete sludge dosage of 10 g/L, Seed dosage (CaF₂) of 2 g/L, mixing intensity of 100 rpm and operation time of 60 min were found to be optimum. Correspondingly, removal of fluoride and water content was about 85% and 64%, respectively. Increase in amount of seed dosage did not affect fluoride removal efficiency. but the result that the water content is decreased was shown up in occuring the solid-liquid separation well.

P278 : Nonablative fractional laser as a tool to facilitate skin penetration of 5-aminolevulinic acid with minimal skin disruption

( In Jung Kang ),( Jeong Hwee Choi ),( Min Jae Gwak ),( Hee Kyeong Lim ),( Mu Hyoung Lee ),( Min Kyung Shin ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Effective penetration of photosensitizer is an essential step in photodynamic therapy (PDT). There have been trials of several methods, including laser treatment, to facilitate prompt and sufficiently deep transdermal drug delivery. Objectives: This study was performed to evaluate the effects of nonablative fractional laser pretreatment on aminolevulinic acid (ALA) penetration of the skin. Methods: Twelve identical treatment areas of 1 × 1 cm2 on the backs of 10 healthy male subjects were mapped. Each area received laser treatment with a different energy and incubation with ALA for different times. Laser treatment was performed with a 1550 nm fractional erbium glass laser, and the laser energy was set to 20 and 50 mJ with a spot density of 50/cm2. ALA incubation time was set to 30, 60, or 180 min. Porphyrin fluorescence was measured. Results: Nonablative fractional laser-pretreated areas showed significantly increased porphyrin fluorescence compared to non-pretreated areas. Laser energy strength and ALA incubation time were positively correlated with ALA absorption. Conclusion: Nonablative fractional laser treatment effectively enhanced ALA skin penetration. Pretreatment with a nonablative fractional laser can be used for ALA-PDT to achieve higher therapeutic efficacy and shortened ALA incubation times with minimal skin barrier disruption compared to ablative laser.

수확 후 산채류의 미생물 제어를 위한 이산화염소수와 유기산 및 Blanching 병합 처리

강지훈(Ji Hoon Kang),박신민(Shin Min Park),김현규(Hyun Gyu Kim),손현정(Hyun Jung Son),이가연(Ka Yeon Lee),강길남(Kil-Nam Kang),박종태(Jong Tae Park),송경빈(Kyung Bin Song) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.2

수확 후 산채류의 미생물학적 안전성을 확보하기 위해 선정된 산채류인 취나물과 곤드레에 이산화염소수와 유기산 용액 병합 처리 및 이산화염소수, 유기산 용액, blanching 병합처리 후 미생물 제어 효과를 비교 분석하였다. 50 ppm 이산화염소수와 0.5% citric acid 용액의 병합 처리는 취나물과 곤드레의 총 호기성 세균 수를 2.80~3.64 log CFU/g, 효모 및 곰팡이 수는 2.02~2.67 log CFU/g 감소시켰다. 50 ppm이산화염소수와 0.5% fumaric acid 용액의 병합 처리 후 총 호기성 세균 수는 대조구와 비교하여 3.62~3.82 log CFU/g 감소하였으며, 효모 및 곰팡이의 경우에는 2.47~3.02 log CFU/g 만큼 감소하여 이산화염소수와 citric acid 용액의 병합 처리보다 fumaric acid 용액과의 병합 처리가 더 효과적인 병합 처리 조건이라고 생각된다. 이산화염소수와 fumaric acid 병합 처리 후 blanching 처리된 취나물의 총 호기성 세균 수는 대조구보다 5.12 log CFU/g 더 낮게 검출되었으며, 효모 및 곰팡이는 검출되지 않았다. 곤드레의 경우에도 효모 및 곰팡이는 검출되지 않았으며, 총 호기성 세균 수는 대조구와 비교하여 4.59 log CFU/ g 감소하였다. 따라서 본 연구 결과 이산화염소수와 유기산 용액 전처리 후 blanching 병합 처리가 산채류의 미생물학적 안전성을 확보하는 가장 효과적인 방법이라고 판단된다. To improve the microbiological safety of wild vegetables after harvest, Aster scaber and Cirsium setidens Nakai were treated with combinations of 50 ppm aqueous chlorine dioxide (ClO₂)/0.5% citric acid or fumaric acid, and 50 ppm ClO₂/0.5% fumaric acid/blanching at 90°C for 2 min. Combined treatment of 50 ppm ClO₂ and 0.5% citric acid reduced populations of total aerobic bacteria, yeast, and molds in Aster scaber and Cirsium setidens Nakai by 2.80∼3.64 and 2.02∼2.67 log CFU/g, respectively, compared to those of the control. Combined treatment of 50 ppm ClO₂ and 0.5% fumaric acid reduced total aerobic bacteria, yeast and molds populations by 3.62∼3.82 and 2.47∼3.02 log CFU/g, respectively. Based on the results, combined treatment of ClO₂ and fumaric acid was more effective in controlling microorganisms in the wild vegetables than either ClO₂ or citric acid. In addition, combined treatment of ClO₂/fumaric acid/blanching reduced the populations of total aerobic bacteria by 4.59∼5.12 log CFU/g, and populations of yeast and molds were not detected by treatment. These results suggest that combined treatment of ClO₂/fumaric acid/blanching is the most effective method for improving microbiological safety of wild vegetables after harvest.

Involvement of calcium-mediated apoptotic signals in H₂O₂-induced MIN6N8a cell death

Choi, Sung-E,Min, Se-Hee,Shin, Ha-Chul,Kim, Hyo-Eun,Jung, Min Whan,Kang, Yup Elsevier 2006 european journal of pharmacology Vol.547 No.1

<P><B>Abstract</B></P><P>Reactive oxygen species are believed to be the central mediators of beta-cell destruction that leads to type 1 and 2 diabetes, and calcium has been reported to be an important mediator of beta cell death. In the present study, the authors investigated whether Ca<SUP>2+</SUP> plays a role in hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>)-induced MIN6N8a mouse beta cell death. Treatment with low concentration H<SUB>2</SUB>O<SUB>2</SUB> (50?μM) was found to be sufficient to reduce MIN6N8a cell viability by 55%, largely via apoptosis. However, this H<SUB>2</SUB>O<SUB>2</SUB>-induced cell death was near completely blocked by pretreatment with BAPTA/AM (5?μM), a chelator of intracellular Ca<SUP>2+</SUP>. Moreover, the intracellular calcium store channel blockers, such as, xestospongin c and ryanodine, significant protected cells from 50?μM H<SUB>2</SUB>O<SUB>2</SUB>-induced cell death and under extracellular Ca<SUP>2+</SUP>-free conditions, 50?μM H<SUB>2</SUB>O<SUB>2</SUB> elicited transient [Ca<SUP>2+</SUP>]<SUB>i</SUB> increases. In addition, pharmacologic inhibitors of calpain, calcineurin, and calcium/calmodulin-dependent protein kinase II were found to have a protective effect on H<SUB>2</SUB>O<SUB>2</SUB>-induced death. Moreover, H<SUB>2</SUB>O<SUB>2</SUB>-induced apoptotic signals, such as c-JUN N-terminal kinase activation, cytochrome <I>c</I> release, caspase 3 activation, and poly (ADP-ribose) polymerase cleavage were all down-regulated by the intracellular Ca<SUP>2+</SUP> chelation. These findings show that [Ca<SUP>2+</SUP>]<SUB>i</SUB> elevation, possibly due to release from intracellular calcium stores and the subsequent activation of Ca<SUP>2+</SUP>-mediated apoptotic signals, critically mediates low concentration H<SUB>2</SUB>O<SUB>2</SUB>-induced MIN6N8a cell death. These findings suggest that a breakdown of calcium homeostasis by low level of reactive oxygen species may be involved in beta cell destruction during diabetes development.</P>

Flavobacterium meningosepticum에 의한 균혈증 1예

장성원,유진홍,진승원,김선우,김연식,박순민,이대훈,최민호,최주연,신완식,강문원,강지민 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.1

1996년 6월 카톨릭대학교 부천성가병원에 뇌경색으로 입원했던 61세 남자 환자의 혈액에서 Flavobacterium meningosepticum을 분리하였다. 환자는 폐렴을 동반한 패혈증의 예로 ceftriaxone과 metronidazole을 투여하였으나 입원 48시간만에 사망하였다. Flabovacterium meningosepticum is a rae cause of nosocomial infection which shows multi-drug resistance. It mainly invades patients with impaired immunity. Recently, we experienced a case of F. meningosepticum bacteremia in a patient with chronic debilitated state owing to stroke. The clinical progress was so rapid that he died within 48 hours. Blood culture revealed F. meningosepticum which showed characteristic yellow colony in blood agar plate.

CFRP 판재의 섬유방향에 따른 절삭성 평가

신봉철,하석재,강민형,권오양,조명우 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

In recent, the CFRP(Carbon Fiber Reinforced Plastics) composite has been widely used because it has light-weight and high mechanical strength. Therefore, CFRP composite is applied to variable industrial areas such as aerospace, automobiles, sporting goods, ships and so on. Generally, CFRP composite has been used in the industrial field by mechanical cutting processes. In this research, the CFRP composite specimen are laminated by 64plies with orientation angles such as 0°, 45°, 90° and [0°/±45°/90°]4S. The results are analyzed with cutting force and cutting surface.

청소년의 자아상과 사회적 민감성간의 관계에 대한 연구 : 자아상-우울-불안-사회적 민감성간의 구조적 관계분석 THE STRUCTURAL RELATION AMONG SELF-IMAGE, DEPRESSION, ANXIETY, AND SOCIAL SENSITIVITY

신민섭,소준현,홍강의 대한소아ㆍ청소년정신의학회 1996 소아청소년정신의학 Vol.7 No.1

한국판 아동용 Luria-Nebraska 신경심리 검사의 표준화 연구 Ⅱ : EVALUATION OF THE VALIDITY & CLINICAL UTILITY OF THE KOREAN VERSION OF LNNB-C 타당도 및 임상적 유용성 검증

신민섭,홍강의 대한소아ㆍ청소년정신의학회 1994 소아청소년정신의학 Vol.5 No.1

요 약 본 연구에서는 다양한 임상 집단과 정상 집단의 아동들을 대상으로 한국판 아동용 Luria- Nebraska 신경심리 검사(LNNB-C)의 타당도와 임상적 유용성에 대해 알아보았다. 8세에서 12세 연령 범위의 뇌손상 집단(N=19), 뇌손상이 의심되는 ADHD 집단(N=16), 소아정신과 장애 집단 (N=16), 그리고 정상 아동 집단(N=147)을 대상으로 한국판 LNNB-C의 진단적 변별력을 검증하였고, BGT, KEDI-WISC와의 상관 관계를 알아보았다. 정상 집단과 뇌손 상 집단간에 LNNB-C 모든 척도상에서 유의미한 차이가 있었으며, 정상 집단과 ADHD 집 단간에도 뇌손상 진단에 중요한 11개 임상 척도와 3개의 요약 척도에서 모두 유의미한 차 이가 있었다. ADHD와 뇌손상 집단간에는 요약 척도(병리 척도, 좌반구 척도 및 우반구 척 도)에서 유의미한 차이가 있었으므로, 요약 척도가 ADHD와 뇌손상 집단을 변별하는데 있 어 중요한 척도임이 시사되었다. 정상 집단과 소아정신과 장애 집단간에는 모든 척도상에서 유의미한 차이가 없었다. LNNB-C 척도점수에 의한 판별 분석 결과, LNNB-C 척도가 전 상 집단의 98.6%를 정확히 정상 집단으로, ADHD집단의 62.5%를 ADHD집단으로, 그리고 뇌손상 집단의 73.7%를 뇌손상 집단으로 정확히 판별하였으며, 전체 정확 판별율이 92.9%였다. 전체 문항들 중에서는 정상 집단과 뇌손상 집단을 유의미하게 변별해주는 문항 이 131개 (87.9%)였으며, 정상과 ADHD 집단을 변별해주는 문항이 55개(36.9%), ADHD와 뇌손상 집단을 구분해주는 문항이 52개(34.9% ), 그리고 ABHD와 소아정신과 집단을 구분 해주는 문항이 23개 (15.4%)인 것으로 나타났다. LNNB-C 임상 척도들과 BCT 오류 점수· 간에 유의미한 정적 상관관계가 있었고, 몇개의 척도를 제외하고는 LNNB-C와 KEDI-WISC 척도 점수들간에도 유의미한 부적 상관이 있는 것으로 나타났으며,특 히 LNNB-C의 지적 과정 척도(Cll)와 FSIQ간에 가장 높은 부적 상관을 보여주었다. 이러 한 결과들은 모두 뇌손상을 진단하는 신경심리 검사로서 한국판 LNNB-C의 타당도 및 진단 변별력이 우수함을 입증해주는 결과라 할 수 있다. ABSTRACT Present study was to evaluate the validity and the clinical utility of the Korean version of Luna-Nebraska Neuropsychological Battery for Children(LNNB-C) in various groups including normal, brain damaged, attention deficit hyperactivity disordered(ADHD), and psychiatrically disordered. The Korean version of LNNB-C and BGT were administered to clinical groups consisted of 51 patients(19 brain damaged, 16 ADHD, and 16 psychiatric controls), and to normal group composed of 147 children between the age of 8 and 12. Also KEDI-WISC was administered to clinical groups as a part of comprehensive psychological assessment There were significant differences between the brain damaged and the normals on all scales of LNNB-C, and between the normals and the ADHD on 11 clinical scales and 3 summary scales,which indicate the clinical validity for the scales of the Korean version of LNNB-C. The significant differences between the ADHD and the brain damaged on 3 summary scales were found,suggesting that the summary scales might play an important role in discriminating between two groups. Multiple discriminant analysis showed that the Korean version of LNNB-C signifi-cantly discriminates 3 groups-normals, ADHD, and brain damaged. Percentages of correct classification were ranged from 62.5% in the ADHD to 98.6% in the normals. For father evaluating the discriminant validity of the LNNB-C, the discriminant power of each items were calculated, and 131 of the 147 items discriminated significantly between the brain damaged and the normals. The scales of LNNB-C significantly correlated with the error scores of BGT and the most of scales of KEDI-WISC. These results put together strongly support the concurrent and the discriminant validity of the Korean version of LNNB-C; in diagnosing brain damage.The limitations of present study and several issues for the father study were discussed.