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Srinivasa Reddy Ronda,Pavani Lakhsmi Chandrika Parupudi,Sandeep Vemula,Santhosh Tumma,Mahendran Botlagunta,Vijaya Saradhi Settaluri,Smita Lele,Suraj Sharma,Chari Kandala 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.10
This work describes the medium optimization of γ-Linolenic acid (GLA) production by Spirulina platensisusing one-factor and orthogonal array design methods. In the one-factor experiments, NaHCO3 (9 mg L−1), NaNO3(13.5 mg L−1) and MgSO4·7H2O (11.85 mg L−1) proved to be the best components for GLA production. The optimalpH for GLA production by the alga was 9.2. Based on the delta values, NaHCO3 showed the greatest effect on the GLAproduction of the various factors tested, followed in decreasing order by MgSO4·7H2O, NaNO3 and K2SO4. The max-imum GLA yield obtained was 19.2 mgL−1in the presence of optimum concentrations of NaHCO3 (20 g L−1), NaNO3(3 g L−1), MgSO4·7H2O (0.5 g L−1) and K2SO4 (1.5 g L−1). Because of the slow growth rate of the algae, the practiceof robust orthogonal array methods during the optimization of medium components can result in the production of anoptimal biomass and a higher GLA yield for nutraceutical applications.