Penicillifer diparietisporus: a New Record from Field Soil in Korea

( Kallol Das ),( Chang-gi Back ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.3

A fungus was isolated from field soil collected from Daegu, Korea. The colony of the isolated fungus showed short, branched, and light to dark yellow pigments with hyaline, yellowish red to orange brown aerial mycelia. In addition, the fungus produced solitary to aggregated perithecia, ovoid to pyriform, short neck, and asci as well as biseriately arranged ascospores. Phylogenetic analysis using the internal transcribed spacer region and translation elongation factor 1-α sequences and morphological characteristics identified the isolated fungus as Penicillifer diparietisporus , which belongs to the family Nectriaceae. To our knowledge, this is the first report of Penicillifer diparietisporus in Korea.

Taxonomy of Arthrinium minutisporum sp. nov., Pezicula neosporulosa, and Acrocalymma pterocarpi: New Records from Soil in Korea

( Kallol Das ),( Seung-yeol Lee ),( Hyo-won Choi ),( Ahn-heum Eom ),( Young-je Cho ),( Hee-young Jung ) 한국균학회 2020 Mycobiology Vol.48 No.6

The strains 17E-042, 17E-039, and NC13-171 belong to Ascomycota and were isolated from soil collected from Sancheong-gun and Yeongam-gun, Korea. The strain 17E-042 produced white mycelial colonies that developed a sienna color with a round margin on potato dextrose agar (PDA), and the reverse side developed a light sienna color. Morphologically, this strain was similar to the strains of Arthrinium phragmites and A. hydei, but the shorter conidial size of the newly identified strain (17E-042) was distinct. The strain 17E-039 produced macroconidia that were pale yellow to orange-brown, elongated-ellipsoid to oblong, round at both ends, primarily straight but sometimes slightly curved, 0-septate, thin-walled, and filled with numerous droplets, having diameters of 20.4-34.3×8.0-12.0 lm. And the strain NC13-171 formed hyaline to light brown chlamydospores, solitary or in a chain. Multigene phylogenetic analyses were conducted using sequence data obtained from internal transcribed spacer (ITS) regions, 28S rDNA large subunit (LSU), b-tubulin (TUB2), translation elongation factor 1-alpha (TEF1-a), and RNA polymerase II large subunit (RPB2) genes. The results of molecular phylogeny, the detailed descriptions and illustrations of each species strongly support our proposal that these strains from soil in Korea be designated as Arthrinium minutisporum sp. nov. and two new records of Pezicula neosporulosa and Acrocalymma pterocarpi.

A New Record on Umbelopsis vinacea and Mucor hiemalis f. corticola Isolated from Korea

( Kallol Das ),( Ki-hun Ha ),( Sang-jae Suh ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2020 韓國菌學會誌 Vol.48 No.3

In the screening of fungal diversity, two strains were collected from the soil of Yeongcheon and dissected guts from the bodies of Chinese rice grasshopper (Oxya chinensis), Chinese grasshopper (Acrida cinerea), and Far eastern devil grasshopper (Oedaleus infernalis) from Daejeon in Korea. They were identified as Umbelopsis vinacea (KNU-YC-1801B) and Mucor hiemalis f. corticola (KNU-20F7, KNU-20F8, KNU-20F9). Multigene phylogenetic analyses of the internal transcribed spacer (ITS) regions, and large subunit (LSU) sequence data confirmed two unreported taxa along with their morphology. The results of molecular phylogeny firmly supported the detailed description and illustration for each taxon. As far as we know, both Umbelopsis vinacea and Mucor hiemalis f. corticola are the first reported taxa in Korea.

Cladophialophora lanosa sp. nov., a New Species Isolated from Soil

( Kallol Das ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2019 Mycobiology Vol.47 No.2

Cladophialophora is characterized by simple hyphomycetes with brown hyphae that give rise to branched chains of pale brown conidia and shows affinities with the Herpotrichiellaceae. A fungal strain belonging to the genus Cladophialophora was isolated from soil in Daegu, Korea. This strain produces numerous greenish to dark black lanose aerial mycelia with hair like structures. It is morphological similar to C. chaetospira, C. inabaensis, and C. multiseptata; however, the conidiophores and conidia sizes of the newly isolated strain (KNU16-032) are clearly different from them. The novelty of the strain was also confirmed based on phylogenetic analysis using the data sets of the internal transcribed spacer region of and the partial sequence of 28S ribosomal DNA region along with the cultural characteristics. Because morphological observations and phylogenetic analysis indicated that the strain is distinct from previously known Cladophialophora species, we propose this species as a new species Cladophialophora lanosa sp. nov., and provide the detailed descriptions in this study.

Nonlinear seismic performance of code designed perforated steel plate shear walls

Kallol Barua,Anjan K. Bhowmick 국제구조공학회 2019 Steel and Composite Structures, An International J Vol.31 No.1

Nonlinear seismic performances of code designed Perforated Steel Plate Shear Walls (P-SPSW) were studied. Three multi-storey (4-, 8-, and 12-storey) P-SPSWs were designed according to Canadian seismic provisions and their performance was evaluated using time history analysis for ground motions compatible with Vancouver response spectrum. The selected code designed P-SPSWs exhibited excellent seismic performance with high ductility and strength. The current code equation was found to provide a good estimation of the shear strength of the perforated infill plate, especially when the infill plate is yielded. The applicability of the strip model, originally proposed for solid infill plate, was also evaluated for P-SPSW and two different strip models were studied. It was observed that the strip model with strip widths equal to center to center diagonal distance between each perforation line could reasonably predict the inelastic behavior of unstiffened P-SPSWs. The strip model slightly underestimated the initial stiffness; however, the ultimate strength was predicted well. Furthermore, applicability of simple shear-flexure beam model for determination of fundamental periods of P-SPSWs was studied.

Unreported Post-harvest Disease of Apples Caused by Plenodomus collinsoniae in Korea

( Kallol Das ),( Yeong-hwan Kim ),( Jingi Yoo ),( Leonid N. Ten ),( Sang-jae Kang ),( In-kyu Kang ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2020 韓國菌學會誌 Vol.48 No.4

This study was conducted to isolate and identify the fungal pathogen caused unreported post-harvest disease on apples (cv. Fuji) fruit in Korea. The disease symptoms on apples appeared as irregular, light to dark brown, slightly sunken spots. The three fungal strains were isolated from infected tissues of apple fruits and their cultural and morphological characteristics were completely consistent with those of Plenodomus collinsoniae. The phylogenetic analysis using the internal transcribed spacer (ITS) regions, beta-tubulin (TUB), and the second largest subunit of RNA polymerase II (RPB 2) sequences revealed the closest relationship of the isolates with Plenodomus collinsoniae at the species level. The pathogenicity test showed the same dark brown spots on Fuji apple cultivar. Therefore, P. collinsoniae is a newly reported fungal agent causing post-harvest disease on apples in Korea.

A New Report on Three Species of Sordariomycetes Class Isolated from Soil in Korea

( Kallol Das ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.2

Three fungal strains belonging to the class Sordariomycetes, namely BH-06, 17-039 and BE12-1, were isolated from soils in Korea and identified as Chaunopycnis alba, Myrothecium cinctum and Humicola olivacea, respectively. These species were confirmed according to their morphological characteristics and phylogenetic relationships determined based on internal transcribed spacer regions, as well as large subunit, small subunit, β-Tubulin and RNA polymerase II largest subunit sequences of ribosomal DNA. These three species are the first members of the Sordariomycetes reported in Korea.

Molecular and Morphological Characterization of Two Novel Species Collected from Soil in Korea

( Kallol Das ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2020 Mycobiology Vol.48 No.1

Two fungal species of ascomycetes were discovered during the screening of soil microflora from the Gangwon Province in Korea: Didymella chlamydospora sp. nov. (YW23-14) and Microdochium salmonicolor sp. nov. (NC14-294). Morphologically, YW23-14 produces smaller chlamydospores (8.0-17.0×7.0-15.0 mm) than D. glomerata and D. musae. The strain NC14- 294 was characterized by smaller conidiogenous cells (4.9-8.8×2.0-3.2 mm) compared with the closest strains M. fisheri and M. phragmitis. The detailed descriptions, illustrations, and discussions regarding the morphological and phylogenetical analyses of the closely related species are provided to support the novelty of each species. Thus, YW23-14 and NC14-294 are described here as newly discovered species.

Pestalotiopsis kaki sp. nov., a Novel Species Isolated from Persimmon Tree (Diospyros kaki) Bark in Korea

( Kallol Das ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2021 Mycobiology Vol.49 No.1

During the screening of Korean microflora, a fungal strain (KNU-PT-1804) belonging to the genus Pestalotiopsis was isolated from persimmon tree (Diospyros kaki) bark collected from North Gyeongsang Province, Korea. The strain, KNU-PT-1804, produced smaller conidia compared with related species P. kenyana, P. neglecta, and P. telopeae. The novelty of the strain was confirmed based on phylogenetic analysis using molecular datasets of internal transcribed spacer (ITS) regions, b-tubulin (TUB2), and translation elongation factor 1-alpha (TEF1a) genes. Molecular phylogeny strongly supports that the strain is distinct from previously known Pestalotiopsis species, and we proposed the novel species, Pestalotiopsis kaki sp. nov., and provide a detailed description and illustration.

A New Report of Biscogniauxia petrensis Isolated from Mosquitoes in Korea

( Kallol Das ),( Joung-ho Kim ),( Kwang-shik Choi ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2020 韓國菌學會誌 Vol.48 No.2

A fungal strain designated KNU-WDM2A2 was isolated from mosquitoes in Gimcheon, Korea. The pure culture was transferred to potato dextrose agar (PDA) and synthetic nutrient agar (SNA) media and attained a diameter of 90 mm after 10 days of incubation at 25℃. The colonies were whitish to light pink and cottony to wooly, with an abundant production of aerial mycelia. The strain produced hyaline to slightly yellowish conidiophores that were rough-walled and branched, with conidiogenous cells arising terminally or laterally. Conidia were unicellular, hyaline to light brown, smooth, and oval or ovoid to clavate, with a size of 4.1-6.9×2.5-3.3 μm (n=65). A phylogenetic analysis was conducted using the internal transcribed spacer (ITS) regions and 28S rDNA of large subunit (LSU) sequences, to support the cultural and morphological characteristics. The KNU-WDM2A2 strain was identified here as Biscogniauxia petrensis, new to Korea.