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RISS 인기검색어
- 검색키워드
- 저자 : Kalaji (검색결과 4 건)
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Omar, Samar A.,Elsheery, Nabil I.,Kalaji, Hazem M.,Xu, Zeng-Fu,Song-Quan, Song,Carpentier, Robert,Lee, Choon-Hwan,Allakhverdiev, Suleyman I. 한국식물학회 2012 Journal of Plant Biology Vol.55 No.6
Changes in $H_2O_2$ and the main antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and glutathione reductase (GR), in endospermic and embryonic tissues were studied in developing and artificially dried Jatropha curcas seeds. Immature seeds were desiccation-tolerant at 80 days after flowering, as they were able to germinate fully after artificial drying on silica gel had reduced their water content to 10-12% of fresh weight. In both endospermic and embryonic tissues, $H_2O_2$ level and, consequently, lipid peroxide content, decreased during seed development as well as after artificial dehydration of developing seeds. All examined antioxidant enzymes except DHAR showed a decrease in total activity in mature stages as compared with early stages. Expression analysis of SOD genes revealed that the decrease in total SOD activities was related to the decrease in Cu/Zn-SOD expression, while the continuous activity of SOD during maturation was related to an increase in Mn-SOD expression. Artificial drying resulted in increased SOD and DHAR activity, irrespective of the developmental stage. Our results revealed weak participation of CAT and APX in $H_2O_2$ scavenging, as well as no significant alterations in GR activities either during maturation or after artificial drying. Changes in SOD and GR isoenzyme patterns occurred during maturation-related drying, but not after artificial drying. These results highlight the role of ascorbate-glutathione cycle enzymes (DHAR and GR) in $H_2O_2$ scavenging during maturation or after artificial drying of developing J. curcas seeds.
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Omar, S A,Elsheery, N I,Kalaji, H M,Ebrahim, M K H,Pietkiewicz, S,Lee, C-H,Allakhverdiev, S I,Xu, Zeng-Fu Consultants Bureau [etc.] 2013 Biochemistry Vol.78 No.5
<P>Plant dehydrin proteins (DHNs) are known to be important for environmental stress tolerance and are involved in various developmental processes. Two full-length cDNAs JcDHN-1 and JcDHN-2 encoding two dehydrins from Jatropha curcas seeds were identified and characterized. JcDHN-1 is 764 bp long and contains an open reading frame of 528 bp. The deduced JcDHN-1 protein has 175 a.a. residues that form a 19.3-kDa polypeptide with a predicted isoelectric point (pI) of 6.41. JcDHN-2 is 855 bp long and contains an open reading frame of 441 bp. The deduced JcDHN-2 protein has 156 a.a. residues that form a 17.1-kDa polypeptide with a predicted pI of 7.09. JcDHN-1 is classified as type Y3SK2 and JcDHN-2 is classified as type Y2SK2 according to the YSK shorthand for structural classification of dehydrins. Homology analysis indicates that both JcDHN-1 and JcDHN-2 share identity with DHNs of other plants. Analysis of the conserved domain revealed that JcDHN-2 has glycoside hydrolase GH20 super-family activity. Quantitative real time PCR analysis for JcDHN-1 and JcDHN-2 expression during seed development showed increasing gene expression of both their transcript levels along with the natural dehydration process during seed development. A sharp increase in JcDHN-2 transcript level occurred in response to water content dropping from 42% in mature seeds to 12% in dry seeds. These results indicate that both JcDHNs have the potential to play a role in cell protection during dehydration occurring naturally during jatropha orthodox seed development.</P>
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Samar A. Omar,Nabil I. Elsheery,Hazem M. Kalaji,Zeng-Fu Xu,Song Song-Quan,Robert Carpentier,이춘환,Suleyman I. Allakhverdiev 한국식물학회 2013 Journal of Plant Biology Vol.56 No.4
Changes in H2O2 and the main antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and glutathione reductase (GR), in endospermic and embryonic tissues were studied in developing and artificially dried Jatropha curcas seeds. Immature seeds were desiccation-tolerant at 80 days after flowering, as they were able to germinate fully after artificial drying on silica gel had reduced their water content to 10–12% of fresh weight. In both endospermic and embryonic tissues, H2O2 level and, consequently, lipid peroxide content, decreased during seed development as well as after artificial dehydration of developing seeds. All examined antioxidant enzymes except DHAR showed a decrease in total activity in mature stages as compared with early stages. Expression analysis of SOD genes revealed that the decrease in total SOD activities was related to the decrease in Cu/Zn-SOD expression, while the continuous activity of SOD during maturation was related to an increase in Mn-SOD expression. Artificial drying resulted in increased SOD and DHAR activity, irrespective of the developmental stage. Our results revealed weak participation of CAT and APX in H2O2 scavenging, as well as no significant alterations in GR activities either during maturation or after artificial drying. Changes in SOD and GR isoenzyme patterns occurred during maturation-related drying, but not after artificial drying. These results highlight the role of ascorbate-glutathione cycle enzymes (DHAR and GR) in H2O2 scavenging during maturation or after artificial drying of developing J. curcas seeds.
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Manzer H. Siddiqui,Saud A. Alamri,Mutahhar Y. Al-Khaishany,Mohammed A. Al-Qutami,Hayssam M. Ali,Hala AL-Rabiah,Hazem M. Kalaji 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.6
Due to increasing soil salinity, the world agricultural output is being threatened by the shrinking area offertile land. In the present study, we explored the interactive roles of nitric oxide (NO; 100 μM) and spermidine (SP;200 μM) in ameliorating the effects of salt stress (NaCl; 100 mM) in tomato (Solanum lycopersicum L. var. FiveStar) seedlings. NaCl stress reduced shoot and root length, shoot and root fresh weight, shoot and root dry weightplant-1 and leaf area leaf-1. NaCl stress also suppressed the biosynthesis of photosynthetic pigments (Chlorophyll aand b) and increased proline (Pro) content, membrane damage and lipid peroxidation by inducing reactive oxygenspecies (H2O2 and O2•−) generation in roots and leaves, as well as electrolyte leakage (EL) and malondialdehyde(MDA) accumulation in leaves. However, applying NO and/or SP increased the activities of catalase, peroxidase,superoxide dismutase, glutathione reductase and ascorbate peroxidase and increased photosynthetic pigment (chlorophylla and b) and Pro accumulation, as well as reducing H2O2, O2•− and MDA content and EL, under salt stress. Whentomato plants were treated with NO and SP simultaneously, NO signaling was further enhanced, which was confirmedby the addition of cPTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide; NO scavenger].
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