한국인의 치아우식 조절에 관한 연구 : Ⅲ. 白鼠우치痍患率에 미치는 數種 稀 有元素, 쌀 및 各種 양념의 影響 Ⅲ.EFFECTS OF SEVERAL TRACE ELEMENTS, RICE AND SPICES ON THE CARIES RATE IN RATS

鄭東均,鄭素英,李孝宰,金各均,吳貴玉,李種昕 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1

Two-part experiment was conducted to elucidate the effects of several trace elements, rice and spices on caries rate in Korean albino rats. Rats were selected into two large groups according to their weight ; one group (for part I experiment) consists of rats weighing 140-200gm and the other group (for part II experiment) consists of rats weighing 76-109gm, and the same experiment was conducted independently for both groups. Rats were fed standard cariogenic diet supplemented with rice (21, 53%), capsicum (1, 10%), garlic (0.5, 5 or 10%), phosphorus (2%), sodium chloride (3%, reagent grade), or table salt. Drinking water was tap water containing cold water extract of capsicum (0.1%) alcoholic extract of capsicum (0.1%), water extract of ginseng (2%), fluoride (10, 50 or 100ppm) or 50ppm of Mg, Zn, Mo, Sr, Mn of Fe. The results were as follows. 1. 24.9%, 51.0% and 54.3% decrease in caries score were recorded for rats which drank water containing 10, 50 and 100ppm of fluoride in part I experiment. In part II experiment caries score decreases 51.8% and 56.9% for 10ppm and 50ppm fluoride content. 2. Capsicum-fed rats showed 51.7% (capsicum 1%) and 58.1% (capsicum 10%) decrease in caries score in part I experiment. 21.5% and 33.6% decrcase were recorded for part II experiment. 3. Garlic-fed rats showed 44.5% (garlic 0.5%), 23.0% (garlic 5%) and 53.7% (garlic 10%) decrease in caries score. 4. Sodium chloride-fed rats showed 122.6% (NaCl 3%) increase in caries score. 5. Rats which were fed diet containing 2% Na_2HPO_4 showed 26.7% decrease in caries score and further 41.6% decrease when 10ppm fluoride was given simultaneously. 6. 28.4% and 31.5% decrease in caries score were recorded for rats which drank water containing 50ppm of Mg and Zn.

한국인의 치아우식 조절에 관한 연구 : Ⅳ. 金堤郡 치문국교 학동의 尿中弗素濃度, 班狀齒指數 및 學童居住地域의 飮料水弗素濃度에 關한 硏究 Ⅳ. STUDY ON THE RELATION BETWEEN THE URINARY FLUORIDE CONCENTRATION, THE FLUOROSIS INDEX AND THE FLUORIDE CONCENTRATION OF DRINKING WATER IN BAIKKU-MYUN, KIMJAE-KUN

丁東均,李種昕,李孝宰,金各均,吳貴玉,鄭泰英 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1

The urinary fluoride concentration and the degree of dental fluorosis of all the schoolboys attending Chimoon primary school in Baikku-myun, Kimjae-kun and the fluoride concentration of drinking water of those areas were investigated in an effort to elucidate the relation between them. The urinary fluoride concentration and the fluoride concentration of driking water were measured by fluoride ion electrode, and dental fluorosis was surveyed and analysed by Dean and McKay's and Horowitz's method. 1) The mean urinary fluoride concentration and the mean fluorosis index of all the schoolboys were 1.89ppm (0.12-12.2ppm) and 0.79 (0-1.99). The mean fluoride concentration of drinking water of those areas was 0.61ppm (0.027-2.66ppm). 2) The mean urinary fluoride concentration and the mean fluorosis index of the schoolboys residing in Yookang-ri 5-ku, Baikku-myun (Dongja-po) were the highest (3.23ppm, 1.99) among the statistics for the schoolboys residing in different areas. Incidentally the fluoride concentration of drinking water for that area was also the highest (1.99ppm). 3) The urinary fluoride concentration was two to ten times as high as the fluoride concentration of drinking water regardless of the resident area of schoolboys. 4) There was no significant seasonal change in fluoride concentration of drinking water collected from the well in the resident area of schoolboys. 5) There showed a relatively parallel relationship between the urinary fluoride concentration and the fluorosis index of schoolboys. 6) The urinary fluoride concentration and the fluorosis index of schoolboys tend to increase with age increasing.

골흡수 기전에 관한 연구 : 파골세포의 활성화 기전 MECHANISM OF OSTEOCLAST ACTIVATION

정동균,고재승,김관식,김각균,민병무,김세원 대한구강생물학회 1989 International Journal of Oral Biology Vol.13 No.1

Although the osteoclast has long been recognized to be the cell responsible for bone resorption, little is known of the mechanisms by which its activity is controlled. Recently, it has been suggested that osteoblasts ─ the bone-forming cells ─ seem to be the target cells of PTH, the bone-resorbing hormone, and mediate osteoclastic bone resorption by producing the coupling factor(s). Because bone tissue consists of several types of cells, isolation of distinct bone cell populations is prerequisite for studying the mechanism of bone resorption in cellular level. This experiment was performed ⅰ) to isolate the metabolically distinct bone cell populations from fetal rat calvaria by sequential enzyme digestion and biochemical characterization and ⅱ) to identify the factor(s) produced by osteoblast that stimulate resorption employing organ culture of bone. Calvaria from rat fetus at 19 day of gestation, were sequentially digested by enzyme solution consisted of collagenase, trypsin and EDTA for 10 (population I), 10(II), 10(III), 20(IV) and 20 minutes (V). Each bone cell population was primarily cultured for 6-7 days and effects of PTH, calcitonin and PGE_2 on acid and alkaline phosphatase activity and cAMP level were determined. Basal level of acid phosphatase in populations released early were higher than late population. In contrast, basal level of alkaline phosphatase was reversed. PTH(0.4 unit/ml) increased the acid phosphatase activity only in population I with no effect on alkaline phosphatase. Calcitonin(150ng/ml) had no effect on acid and alkaline phosphatase activity in all bone cell populations. cAMP level of population IV and V were increased by PTH significantly while CT had no effect in all bone cell populations at all. PGE_2 increased cAMP in all populations, the acid phosphatase activity in population I and alkaline phosphatase activity in population IV and V. Taken together, these results indicate that population IV and V express typical osteoblastic phenotype while population I revealed some characteristics of osteoclast. Bone cell population IV and V were incubated with fresh MEM or MEM containing 0.4U/ml PTH for 2 hours. After 2 hour-incubation, both the control-conditioned media(control-CM) or PTH-conditioned media(PTH-CM) were collected. Both conditioned media were lyophyllized and redissolved as 2 fold concentrate. Ulnae and radii were removed from 19-day old fetal rats, prelabelled by subcutaneous injection of 200μCi ^45CaCl_2 into their mothers on the 17th day of gestation. After 24 hours, media was changed with fresh BGJb media or BGJb media containing 300μl of control-CM or PTH-CM and cultured for 5 days. Effects of control-CM or PTH-CM were observed by the ratios of %-release of ^45Ca between paired control and experimental group. Control-CM obtained from population IV and V had no or very little effect on bone resorption but PTH-CM obtained from population IV and V increased the ^45Ca release significantly after 3 and 5 days of culture. This result provides the evidence indicating that osteoblastic cells mediate osteoclastic bone resorption stimulated by PTH.

한국인의 치아우식 조절에 관한 연구 : Ⅱ.흰쥐 치아우식 이환율에 미치는 수종 양념의 영향에 관한 추가연구 Ⅱ. ADDITIONAL STUDIES ON THE EFFECTS OF THE SEVERAL SPICES ON THE CARIES RATE IN RATS

정동균,이종흔,김각균,이공훈,오귀옥,정태영 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1

Those studies were performed to elucidate the effects of several dietary spices on dental caries and to determine which component of spices induced the change of incidence of dental caries in Sprague-Dawley rats which were supplied from SNU animal house. For this study, 132 rats (rats weighing 90-140 gm) were chosen at random with the same number of both sexes and divided into eleven experimental groups which contained the differential components of drinking water and diets for control and experimental group. Control group was fed standard cariogenic diet and tap water. In experimental groups, rats were fed standard cariogenic diet supplemented with capsicum (10%), ash capsicum (1, 10%), garlic (5%), common salt (3%), and analytical salt (3%) for 80 days. Drinking water was tap water containing water extract of capsicum (1, 10%), water extract of garlic (1.1%), and fluoride (50ppm). The results were as follows. 1. 54.6 in caries score was recorded in control group. 2. Rats which drank water containing 50ppm of fluoride showed 18.4% decrease in caries score. 3. Capsicum-fed rats showed 10.7% (capsicum 10%) increase in caries score and rats which drank water containing water extract of capsicum (1, 10%) showed 14.1% (w.e. capsicum 1%) and 0.16% (w.e. capsicum 10%) increase in caries score. But ash capsicum-fed rats showed 18.7% (ash capsicum 1%) and 7.3% (ash capsicum 10%) decrease in caries score. 4. Garlic-fed rats showed 22.1% (garlic 5%) decrease in caries score. But rats which drank water containing 1.1% of water extract of garlic showed 24.1% increase in caries score. 5. 58.4% and 68.0% increase in caries score were recorded in rats which were fed diet containing common salt (3%) and analytical NaCl (3%), respectively.

한국인의 치아우식 조절에 관한 연구 : Ⅰ. 만경·동진강유역 음료수내의 수종 무기질 함량 Ⅰ. THE CONTENTS OF SEVERAL ELEMENTS IN DRINKING WATER AROUND THE REGIONS OF THE MANKYUNG AND DONGJIN RIVERS

이종흔,정동균,김중수,이효재,김각균,오귀옥,정태영 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1

Mineral analyses of water samples from several areas of the Mankyung River and the Dongjin River regions in Chonrabukdo where dental fluorosis was observed, which had been conducted between June in 1978 and January in 1979, have shown the following results. In general, the mean calcium concentrations were high in the areas where the mean concentrations of phosphorus and fluoride were low. And in the same area where dental fluorosis was particularly observed, seasonally a few minerals were variable─namely, calcium, phosphorus, and fluoride. Those samples of high fluoride concentration have higher phosphorus concentration, but calcium concentrations were the opposite phenomenon. Particularly, fluoride concentrations were remarkably greater during the cold season(January) than during the rainy season(July).

한국인의 치아우식 조절에 관한 연구 : Ⅱ. Streptococcus mutans strain B-13이 생산한 불용성 세포외 다당류와 dextransucrase 활성 Ⅱ. INSOLUBLE EXTRACELLULAR POLYSACCHARIDES AND ACTIVITY OF DEXTRANSUCRASE PRODUCED BY STREPTOCOCCUS MUTANS STRAIN B-13, IN VITRO

정태영,정동균,이효재,김각균,오귀옥,이종흔 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1

Insoluble extracellular polysaccharides synthesized by Streptococcus mutans type d strain B-13 were analysed in an effort to elucidate their chemical composition. Dextransucrase produced in supernatant was also studied for its activity. 1. The microorganism produced 2.10g of total insoluble fraction which contained 1.59g of insoluble polysaccharide and 0.41g of bacterial mass per 800ml of culture medium. 2. Insoluble fraction contained 26.1㎍/mg of nitrogen and 746.4㎍/mg of insoluble extracellular polysaccharides. Ketohexose content of polysaccharides was 7.12%. 3. Streptococcus mutans strain B-13 was capable of producing 4.10g insoluble extracellular polysaccharide per 1.0g of bacterial mass. 4. Dextransucrase liberated 5.11mg of hexose per 1ml of culture supernatant.

한국인의 치아우식 조절에 관한 연구 : Ⅳ. 고추수침엑스가 Streptococcus mutans B-13의 성장에 미치는 영향 Ⅳ. THE EFFECT OF WATER-EXTRACT OF CAPSICUM ON THE GROWTH OF STREPTOCOCCUS MUTANS B-13

이종흔,정동균,정태영,김각균,민병무,이공훈 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1

This experiment was performed to elucidate the reported anti-cariogenic effect of capsicum in rats. Streptococcus mutans strain B-13, which was drived from human oral cavity and known to be cariogenic, was cultured in TYG with water-extract of capsicum. Glucose content of water-extract of capsicum was also determined by glucose oxidase method. The results are as follows. 1. Glucose content of water-extract of capsicum was 25.6 (±0.5) %. 2. In logarithmic phase, water-extract of capsicum group showed less steepness in growth curve than control group. 3. In early stationary phase, size of bacterial cell mass was in order of control, 0.5% and 1% water-extract of capsicum group. 4. It appears that water-extract capsicum might contain inhibitory substance to growth of Streptococcus mutans B-13, in spite of its high glucose content.

유전자재조합 인간 골형성단백2 및 생흡수성고분자를 이용한 골형성유도체의 개발

이장희,김각균,안강민,김종원,이종호 大韓顎顔面成形再建外科學會 1999 Maxillofacial Plastic Reconstructive Surgery Vol.21 No.4

We tested the bone regenerating capacity and histologic response of bioresorbable matrix-type implant, which was made with Poly(lactide-co-glycolide)(PLGA) and bone apatite for the carrier of bone morphogenetic protein(BMP). The critical size defect of 8mm in diameter was created at the calvaria of SD rats(n=18), and repaired with polymer implant with 15㎍ of rhBMP-2(n=9) or without it(n=9). At 2 weeks, 1 months after implantation, the animals were sacrified(3 animals at every interval and group) and histologically evaluted. The calvarial defect which was repaired with polymer with BMP healed with newly formed bone about 70% of total defect. But that without BMP showed only 0 to under 30% bony healing. Inflammtory response was absent in both group through the experimental period, but there's marked foreign body giant response though it was a little less significant in polymer with BMP group. As the polymer was resorbed, the space was infiltrated and replaced by fibrovascular tissue, not by bone. In conclusion, our formulation of bioresorbable matrix implant loaded with bone morphogenetic protein works good as a bone regenerating material. However, it is mandatory to devise our system to have better osteoinductive and osteoconductive property, and less mutinucleated giant cell response.

고추수침엑스가 Streptococcus mutans B-13의 세포외 다당류 합성에 미치는 영향에 관한 전자현미경적 연구

고재승,정태영,이종흔,정동균,김각균 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1

Streptococcus mutans B-13 was cultured in sucrose broth containing water-extract of capsicum and the effect of the extract was observed with the aid of electron microscope, in an effort to elucidate the anti-cariogenic effect of capsicum in white rats observed during the previous experiment. Growth curve was also obtained in the presence or in the absence of the extract. The results were as follows : 1. S. mutans B-13 produce no extracellular polysaccharide in glucose broth. 2. In sucrose broth, extracellular polysaccharides with two components were produced ; that is, electron-dense, globular and relatively homogenous material and widespread fibrillar component. 3. In sucrose broth containing water-extract of capsicum, production of extracellular polysaccharide was almost completely suppressed. Growth was also influenced by the extract. 4. It seems that certain material which is capable of suppressing the production of extracellular polysaccharide and of influencing growth is present in water-extract of capsicum and that anti-cariogenic effect of capsicum is probably related to this unidentified material.

Whole-genomic DNA probe를 이용한 구강 Streptococcus 균종의 식별

이영호,이장희,김각균,최선진 대한구강생물학회 1992 International Journal of Oral Biology Vol.16 No.1

DNA hybridization with whole-genomic DNA probe and restriction pattern analysis were done in search for a useful method for identification of the species of mutans group streptocci. Dot blots of genomic DNA of five strains (serotypes a, b, c and g) of mutans streptococci and on strain of Streptococcus sanguis were hybridized with ^32P-labeled whole-genomic DNA of Streptococcus mutans strain 10449 (serotype c) as a probe. Also, genomic DNAs of each strain were digested with two restriction enzymes (EcoRI and HindIII) and the restriction patterns were compared on 0.7% agarose gel after electrophoresis. Whole-genomic DNA probe of Strep. mutans strain 10449 was able to detect 3ng of homologous DNA per dot and was also able to detect the same amount of genomic DNA of a different strain of the same serotype with about the same intensity as shown on the autoradiogram. Genomic DNAs of differnet serotypes of mutans streptococci and of Strep. sanguis strain MPC1 were hybridized weakly with the probe at 50-100ng per dot. Genomic DNAs of six bacteria of two differnet genera (Bacteroides gingivalis and intermedius) were not hybridized with the probe at any concentration used in experiment. Restriction-digested patterns of genomic DNAs were all different from strain to strain, indicating genomic structures between each serotypes of mutans streptococci.