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      • KCI등재

        Numerical simulation of bubble separation length in a gas–liquid separator based on the Euler–Lagrange method

        Tingting Zhang,Junlian Yin,Dezhong Wang 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.11

        In this study, the bubble separation behavior in a gas–liquid separator is numerically investigated on the basis of the Euler–Lagrange approach, in which the forces acting on bubbles in a swirling flow field are modeled to calculate the trajectories of the bubbles. By adopting this approach, the effects of five parameters, namely, back pressure, Reynolds number, bubble diameter, void fraction, and swirl number, on separation performance in terms of pressure loss, separation efficiency, separation length, and split ratio are computed and analyzed. On the basis of the analysis, correlations of separation length with the two main parameters are established, which can serve as a basis for the optimal design of separator.

      • SCOPUSKCI등재

        The Effect of Different Inflows on the Unsteady Hydrodynamic Characteristics of a Mixed Flow Pump

        Yun, Long,Dezhong, Wang,Junlian, Yin,Youlin, Cai,Chao, Feng Korean Society for Fluid machinery 2017 International journal of fluid machinery and syste Vol.10 No.2

        The problem of non-uniform inflow exists in many practical engineering applications, such as the elbow suction pipe of waterjet pump and, the channel head of steam generator which is directly connect with reactor coolant pump. Generally, pumps are identical designs and are selected based on performance under uniform inflow with the straight pipe, but actually non-uniform suction flow is induced by upstream equipment. In this paper, CFD approach was employed to analyze unsteady hydrodynamic characteristics of reactor coolant pumps with different inflows. The Reynolds-averaged Naiver-Stokes equations with the $k-{\varepsilon}$ turbulence model were solved by the computational fluid dynamics software CFX to conduct the steady and unsteady numerical simulation. The numerical results of the straight pipe and channel head were validated with experimental data for the heads at different flow coefficients. In the nominal flow rate, the head of the pump with the channel head decreases by 1.19% when compared to the straight pipe. The complicated structure of channel head induces the inlet flow non-uniform. The non-uniformity of the inflow induces the difference of vorticity distribution at the outlet of the pump. The variation law of blade to blade velocity at different flow rate and the difference of blade to blade velocity with different inflow are researched. The effects of non-uniform inflow on radial forces are absolutely different from the uniform inflow. For the radial forces at the frequency $f_R$, the corresponding amplitude of channel head are higher than the straight pipe at $1.0{\Phi}_d$ and $1.2{\Phi}_d$ flow rates, and the corresponding amplitude of channel head are lower than the straight pipe at $0.8{\Phi}_d$ flow rates.

      • KCI등재

        The MYB transcription factor StMYBA1 from potato requires light to activate anthocyanin biosynthesis in transgenic tobacco

        Yuhui Liu,Li Wang,Junlian Zhang,Bin Yu,Jing Wang,Di Wang 한국식물학회 2017 Journal of Plant Biology Vol.60 No.1

        StMYBA1 from potato tuber was transformed into tobacco (Nicotiana tabacum) with the CaMV 35S promoter to investigate whether it can regulate anthocyanin biosynthesis in tobacco plants. As a result, anthocyanin accumulation was induced by overexpressing StMYBA1 in all tissues of transgenic tobacco lines. Several anthocyanin biosynthetic genes were highly expressed in leaves and roots of transgenic lines. In particular, the bHLH transcriptional partners NtAN1a and NtAN1b, especially NtAN1b, were up-regulated significantly in leaves and roots of StMYBA1-overexpressing tobacco. These results demonstrate that endogenous bHLH partners can be stimulated by overexpressing exogenous gene StMYBA1 in tobacco, and the elevated expression levels of bHLH partners are essential for anthocyanin production in plant tissues. Applying the dark conditions to the tobacco plants overexpressing StMYBA1 transiently, can down-regulate the expression levels of biosynthetic pathway genes and bHLH TFs, and subsequently reduce anthocyanin accumulation. We suggest that StMYBA1 can positively regulate anthocyanin biosynthesis in tobacco, and light is required for its function on anthocyanin accumulation.

      • KCI등재

        The Endophytic Bacteria Bacillus velezensis Lle-9, Isolated from Lilium leucanthum, Harbors Antifungal Activity and Plant Growth-Promoting Effects

        Mohammad Sayyar Khan,Junlian Gao,Xuqing Chen,Mingfang Zhang,Fengping Yang,Yunpeng Du,The Su Moe,Iqbal Munir,Jing Xue,Xiuhai Zhang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.5

        Bacillus velezensis is an important plant growth-promoting rhizobacterium with immense potential in agriculture development. In the present study, Bacillus velezensis Lle-9 was isolated from the bulbs of Lilium leucanthum. The isolated strain showed antifungal activities against plant pathogens like Botryosphaeria dothidea, Fusarium oxysporum, Botrytis cinerea and Fusarium fujikuroi. The highest percentage of growth inhibition i.e., 68.56±2.35% was observed against Fusarium oxysporum followed by 63.12 ± 2.83%, 61.67 ± 3.39% and 55.82 ± 2.76% against Botrytis cinerea, Botryosphaeria dothidea, and Fusarium fujikuroi, respectively. The ethyl acetate fraction revealed a number of bioactive compounds and several were identified as antimicrobial agents such as diketopiperazines, cyclo-peptides, linear peptides, latrunculin A, 5α-hydroxy-6-ketocholesterol, (R)-S-lactoylglutathione, triamterene, rubiadin, moxifloxacin, 9-hydroxy-5Z,7E,11Z,14Zeicosatetraenoic acid, D-erythro-C18-Sphingosine, citrinin, and 2- arachidonoyllysophosphatidylcholine. The presence of these antimicrobial compounds in the bacterial culture might have contributed to the antifungal activities of the isolated B. velezensis Lle- 9. The strain showed plant growth-promoting traits such as production of organic acids, ACC deaminase, indole-3-acetic acid (IAA), siderophores, and nitrogen fixation and phosphate solubilization. IAA production was accelerated with application of exogenous tryptophan concentrations in the medium. Further, the lily plants upon inoculation with Lle-9 exhibited improved vegetative growth, more flowering shoots and longer roots than control plants under greenhouse condition. The isolated B. velezensis strain Lle-9 possessed broad-spectrum antifungal activities and multiple plant growth-promoting traits and thus may play an important role in promoting sustainable agriculture. This strain could be developed and applied in field experiments in order to promote plant growth and control disease pathogens.

      • SCIESCOPUSKCI등재

        Ginsenoside Rb1 and compound K improve insulin signaling and inhibit ER stress-associated NLRP3 inflammasome activation in adipose tissue

        Chen, Weijie,Wang, Junlian,Luo, Yong,Wang, Tao,Li, Xiaochun,Li, Aiyun,Li, Jia,Liu, Kang,Liu, Baolin The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.4

        Background: This study was designed to investigate whether ginsenoside Rb1 (Rb1) and compound K (CK) ameliorated insulin resistance by suppressing endoplasmic reticulum (ER) stress-induced inflammation in adipose tissue. Methods: To induce ER stress, epididymal adipose tissue from mice or differentiated 3T3 adipocytes were exposed to high glucose. The effects of Rb1 and CK on reactive oxygen species production, ER stress, TXNIP/NLRP3 inflammasome activation, inflammation, insulin signaling activation, and glucose uptake were detected by western blot, emzyme-linked immunosorbent assay, or fluorometry. Results: Rb1 and CK suppressed ER stress by dephosphorylation of $IRE1{\alpha}$ and PERK, thereby reducing TXNIP-associated NLRP3 inflammasome activation in adipose tissue. As a result, Rb1 and CK inhibited IL-$1{\beta}$ maturation and downstream inflammatory factor IL-6 secretion. Inflammatory molecules induced insulin resistance by upregulating phosphorylation of insulin receptor substrate-1 at serine residues and impairing insulin PI3K/Akt signaling, leading to decreased glucose uptake by adipocytes. Rb1 and CK reversed these changes by inhibiting ER stress-induced inflammation and ameliorating insulin resistance, thereby improving the insulin IRS-1/PI3K/Akt-signaling pathway in adipose tissue. Conclusion: Rb1 and CK inhibited inflammation and improved insulin signaling in adipose tissue by suppressing ER stress-associated NLRP3 inflammation activation. These findings offered novel insight into the mechanism by which Rb1 and CK ameliorate insulin resistance in adipose tissue.

      • SCIESCOPUSKCI등재

        Experiment and modeling of liquid-phase flow in a venturi tube using stereoscopic PIV

        Song, Yuchen,Shentu, Yunqi,Qian, Yalan,Yin, Junlian,Wang, Dezhong Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.1

        Venturi tube is based on turbulent flow, whereby the microbubbles can be generated by the turbulent fragmentation. This phenomenon is common in several venturi bubblers used by the nuclear, aerospace and chemical industries. The first objective of this paper is to study the liquid-phase velocity field experimentally and develop correlations for the turbulent quantities. The second objective is to research velocity field characteristics theoretically. Stereoscopic PIV measurements for the velocity field have been analyzed and utilized to develop the turbulent kinetic energy in the venturi tube. The tracking properties of the tracer particles have been verified enough for us to analyze the turbulence field. The turbulence kinetic energy has a bimodal distribution trend. Also, the results of turbulence intensity along the horizontal direction is gradually uniform along the downstream. Both the mean velocity and the fluctuation velocity are proportional to the Reynolds number. Besides, the distribution trend of the mean velocity and the velocity fluctuation can be determined by the geometric parameters of the venturi tube. An analytical function model for the flow field has been developed to obtain the approximate analytical solutions. Good agreement is observed between the model predictions and experimental data.

      • KCI등재

        Experimental and numerical investigation on the pressure pulsation in reactor coolant pumps under different inflow conditions

        Huang Song,Song Yu,Yin Junlian,Xu Rui,Wang Dezhong 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.4

        A reactor coolant pump (RCP) is essential for transporting coolant in the primary loop of pressurized water reactors. In the advanced passive reactor, the absence of a long pipeline between the steam generator and RCP serves as a transition section, resulting in a non-uniform flow field at the pump inlet. Therefore, the characteristics of the pump should be investigated under non-uniform flow to determine its influence on the pump. In this study, the pressure pulsation characteristics were examined in the time and frequency domains, and the sources of low-frequency and high-amplitude signals were analyzed using wavelet coherence analysis and numerical simulation. From computational fluid dynamics (CFD) results, non-uniform inflow has a great effect on the flow structures in the pump's inlet. The pressure pulsation in the pump at the rated flow increased by 78–128.7% under the non-uniform inflow condition in comparison with that observed under the uniform inflow condition. Furthermore, a low-frequency signal with a high amplitude was observed, whose energy increased significantly under non-uniform flow. The wavelet coherence and CFD analysis verified that the source of this signal was the low-frequency pulsating vortex under the steam generator.

      • KCI등재

        The Effect of Different Inflows on the Unsteady Hydrodynamic Characteristics of a Mixed Flow Pump

        Long Yun,Wang Dezhong,Yin Junlian,Cai Youlin,Feng Chao 한국유체기계학회 2017 International journal of fluid machinery and syste Vol.10 No.2

        The problem of non-uniform inflow exists in many practical engineering applications, such as the elbow suction pipe of waterjet pump and, the channel head of steam generator which is directly connect with reactor coolant pump. Generally, pumps are identical designs and are selected based on performance under uniform inflow with the straight pipe, but actually non-uniform suction flow is induced by upstream equipment. In this paper, CFD approach was employed to analyze unsteady hydrodynamic characteristics of reactor coolant pumps with different inflows. The Reynolds-averaged Naiver-Stokes equations with the k-ε turbulence model were solved by the computational fluid dynamics software CFX to conduct the steady and unsteady numerical simulation. The numerical results of the straight pipe and channel head were validated with experimental data for the heads at different flow coefficients. In the nominal flow rate, the head of the pump with the channel head decreases by 1.19% when compared to the straight pipe. The complicated structure of channel head induces the inlet flow non-uniform. The non-uniformity of the inflow induces the difference of vorticity distribution at the outlet of the pump. The variation law of blade to blade velocity at different flow rate and the difference of blade to blade velocity with different inflow are researched. The effects of non-uniform inflow on radial forces are absolutely different from the uniform inflow. For the radial forces at the frequency fR, the corresponding amplitude of channel head are higher than the straight pipe at 1.0Фd and 1.2Фd flow rates, and the corresponding amplitude of channel head are lower than the straight pipe at 0.8Фd flow rates.

      • KCI등재

        Numerical and experimental investigation on the diffuser optimization of a reactor coolant pump with orthogonal test approach

        Long Yun,Zhu Rongsheng,Wang Dezhong,Yin Junlian,Li Tianbin 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.11

        The diffuser of a reactor coolant pump was optimized using an orthogonal approach with numerical simulation to improve the pump hydraulic performance. Steady simulation was conducted by solving Reynolds-averaged Naiver-Stokes equations with the SST k-ω turbulence model using CFX code. The influence of the diffuser geometric parameters, namely, S, φ, α 4 , b 4 , δ 2 , R t and R 4 , on the pump performance were determined. L 18 (3 7 ) orthogonal table was chosen for the optimization process. Best indicators were determined, and range analysis of energy losses, head, and efficiency at the rated condition was performed. Optimal parameters of the diffuser were S = 490 mm, φ = 36°, α 4 = 30°, b 4 = 200 mm, δ 2 = 20 mm, R t = 5 mm and R 4 = 565 mm. The final design was experimentally tested. Simulation results showed more remarkable performance than the experimental result. However, the numerical predictions and experimental results were consistent, validating the design procedure. Loading of the impeller and diffuser blades was analyzed to investigate the direct impact on the hydrodynamic flow field. The head was 14.74 m, efficiency was 79.6 %, and efficiency of the prototype pump was 83.3 % when the model pump functioned at the rated conditions. Optimization results showed that efficiency and head were improved at the design condition.

      • Weak magnetic field accelerates chromate removal by zero-valent iron

        Feng, Pian,Guan, Xiaohong,Sun, Yuankui,Choi, Wonyong,Qin, Hejie,Wang, Jianmin,Qiao, Junlian,Li, Lina Elsevier 2015 Journal of environmental sciences Vol.31 No.-

        <P>Weak magnetic field (WMF) was employed to improve the removal of Cr(VI) by zero-valent iron (ZVI) for the first time. The removal rate of Cr(VI) was elevated by a factor of 1.12-5.89 due to the application of a WMF, and the WMF-induced improvement was more remarkable at higher Cr(VI) concentration and higher pH. Fe2+ was not detected until Cr(VI) was exhausted, and there was a positive correlation between the WMF-induced promotion factor of Cr(VI) removal rate and that of Fe2+ release rate in the absence of Cr(VI) at pH4.0-5.5. These phenomena imply that ZVI corrosion with Fe2+ release was the limiting step in the process of Cr(VI) removal. The superimposed WMF had negligible influence on the apparent activation energy of Cr(VI) removal by ZVI, indicating that WMF accelerated Cr(VI) removal by ZVI but did not change the mechanism. The passive layer formed with WMF was much more porous than without WMF, thereby facilitating mass transport. Therefore, WMF could accelerate ZVI corrosion and alleviate the detrimental effects of the passive layer, resulting in more rapid removal of Cr(VI) by ZVI. Exploiting the magnetic memory of ZVI, a two-stage process consisting of a small reactor with WMF for ZVI magnetization and a large reactor for removing contaminants by magnetized ZVI can be employed as a new method of ZVI-mediated remediation.</P>

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