Protective Effects of Portulaca oleracea L. Extract against Matrix Metalloproteinase Production and Reactive Oxygen Species Generation Induced by Ultraviolet B Radiation in Human Keratinocytes

Jung Hwan Oh(오정환),Fatih Karadeniz(파티 카라데니즈),Jung Im Lee(이정임),So Young Park(박소영),Youngwan Seo(서영완),Chang-Suk Kong(공창숙) 한국생명과학회 2018 생명과학회지 Vol.28 No.8

쇠비름(Portulaca oleracea.L)은 쇠비름과에 속하는 한해살이풀로서 리놀렌산과 같은 불포화지방산, 페놀성 화합물, 플라보노이드, 비타민 C, 미네랄 함량이 높은 것으로 보고되어 있다. 본 연구에서는 쇠비름 추출물을 이용하여 UVB를 조사한 인간각질형성세포에서 광노화 억제능을 확인하였다. Matrix metalloproteinases는 세포의 기질을 분해하는 효소로 MMP-1는 collagenase, MMP-2와 MMP-9는 gelatinases로 피부 진피층을 구성하는 type Ⅰ collagen을 분해시키는데 영향을 미친다. UVB를 조사한 인간각질형성세포에서 쇠비름 추출물을 처리했을 때 MMP-1, -2, -9의 발현이 감소하였으며, type Ⅰ procollagen의 발현은 증가하는 것으로 나타났다. 또한 쇠비름 추출물을 처리한 군에서 UV에 의한 ROS 생성이 감소하였는데 이는 Nrf-2의 활성화를 통한 항산화 인자 SOD-1과 OH-1의 발현 증가로 인해 세포내 ROS 생성이 감소한 것으로 사료된다. 따라서 본 연구 결과를 통해 쇠비름 추출물이 UVB를 조사한 인간각질형성세포에서 MMP 인자 및 항산화 인자의 발현 조절을 통해 광노화로부터의 세포 보호능을 가지는 것을 확인하였으며 나아가 화장품 소재로서의 개발 가능성을 확인하였다. Portulaca oleracea L. is an edible plant widely consumed in daily diet throughout Europe, Asia and America. In this study, protective effects of P. oleracea L. extracts against oxidative stress and matrix metalloproteinase (MMP) activity induced by ultraviolet B (UVB) radiation were investigated using HaCaT immortal human keratinocytes. In this context, the mRNA and protein productions of MMPs (MMP-1, -2, and -9) and type I procollagen, which are major markers of photoaging induced by UVB radiation in HaCaT keratinocytes, were evaluated. Furthermore, UVB-induced reactive oxygen species (ROS) generation and mRNA and protein expression levels of superoxide dismutase-1 (SOD-1), oxygenase-1 (OH-1), and nuclear factor-erythroid 2-related factor-2 (Nrf-2), all of which are associated with the antioxidant balance, were investigated. As shown by the results, UVB radiation induced ROS formation and led to increased production of MMPs and decreased collagen production in human keratinocytes, which resulted in skin photoaging or photodamage. The treatment with P. oleracea L. extracts downregulated MMP (MMP-1, -2, and -9) production and upregulated type I procollagen expression in UVB-induced HaCaT cells. Furthermore, treatment with the extracts decreased UVB-induced ROS generation and increased the expression of antioxidant enzymes, such as SOD-1 and OH-1, through the Nrf-2 pathway. Taken together, these results suggest that P. oleracea L. extracts could be a potential cosmeceutical agent for the prevention of skin photoaging or photodamage.

췌장염에 의한 가성낭종내에 생긴 가성동맥류 : 1례 보고

오연희,이채경,김승현,이성우,양창현,이정호,이영현 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

만성 췌장염의 합병증으로 생긴 가성동맥류는 드문질환으로, 반복적으로 다량의 출혈을 일으킬 수 있으며, 치료를 하지 않을 경우 사망율이 높기 때문에, 조기 진단과 치료가 필수적이다. 저자들은 38세 남자 환자에서 췌장염의 합병증으로 생긴 가성낭종내에서 발생한 가성동맥류 1예를 경험하였기에 보고하는 바이다. 가성 동맥류의 색혈류도플러상 낭종내에 양방향 흐름의 와류를 볼 수 있었다. Pancreatic Pseudoaneurysm within Pseudocyst from Pancreatitis: 1 Case Department of Diagnostic Radiology and Internal Medicine, College of Medicine,DongGuk University Yeon Hee OH M.D., Chae Kyeong Lee M.D., Seoung Hyeon Kim M.D., Sung Woo Lee M.D., Chang Heon Yang M.D., Jung Ho Lee M.D., Young Hyun Lee M.D. Pseudoaneurysm from pancreatitis is uncommon, but it can cause recurrent and massive hemorrhage. Because of high morbidity and mortality, early detection and treatment of the pseudoaneurysm is needed. We report a case of pseudoaneurysm within pseudocyst from pancreatitis. Color-flow Doppler sonography shows bidirectional flow and turbulent arterial flow, within anechoic mass.

N_3O_2형의 Dibenzoic Polyether Nitrogen과 Fe족 금속이온의 錯物에 關한 硏究(Ⅰ)

丁學鎭,丁梧鎭,曺正煥 조선대학교 기초과학연구소 1984 自然科學硏究 Vol.7 No.1

새로운 N_2O_3형 Dibenzonic Polyether Nitrogen을 합성하여 여러 비양자성 용매하에서〔Acetonitrile (AN), N, N-Dimethylformamide(DMF), Dimethylsulfoxide(DMSO) 및 Tetrahydrofuran(THF)와 Fe족 금속이온염과 합성한 Ligandrks의 착물생성에 따른 안정도 상수를 전기전도도법으로 구하고 착물생성으로 유발되는 음이온효과 용매효과 등을 UV-Spectrum으로부터 알아보았다. 그리고 비양자성 용매하에서의 전기전도도 자료로부터 착물이온들의 행동을 검토하였다. Dibenzonic Polyether Nitrogen (DBPN)의 구조는 원소분석과 IR-Spectrum 자료로부터 확인하였다. DBPN-CoCl_2DBPN-NiCl_2DBPN-Co(NO_3)_2 및 DBPN-Ni(NO_3)_2 착이온들의 비전도도를 Tokyo Electric Conductivitymeter에 의하여 25℃에서 측정하였다. 용매의 첨가량에 따라 Complex ion들의 당량전도도는 용매효과 금속양이온 및 음이온 등에 의하여 영향을 받는다. 특히 DBPN-metal(Co^2+, Ni^2+) Complex의 안정도상수 값은 AN>DMSO>THF 순이다. 일정 용매하에서 DBPN-metal(Co^2+, Ni^2+) 착물들의 안정도정수들은 Co^2+>Ni^2+와 Cl^->NO_3^-의 순으로 증가하였다. 이런 결과들로부터 DBPN-Ni과 Co의 착물들의 안정도정수는 DBPN의 동공 크기와 양이온크기의 상대적인 비에 의하여 크게 의존하였다. 그리고 비양자성 용매하에서 Complex ion들의 최대흡광 Peak는 핵전이 장파장이동 Peak를 보여준다. The ligand of the new Dibenzoic Polyether Nitrogen(DBPN) or the N_3O_2 type has been synthezised. The stability constants for the 1‥1 complexes of DBPN with cobalt and nickel in Acetonitrile, Dimethylsulfoxide, Tetrahydrofrane and and N, N-Dimethylforamide have been determined by electrical conductivitimeter and the effect of solvents, cations and anions indused from formation of complexes with DBPN ligand, have been discussed by the data of the UV-spectrum, and then the behaviors of these complexes ions have been discussed from the data of analysis of elements and IR-spectrum. The structures of DBPN are indentified from the data of analysis of elements and IR-spectrum. The specific conductances of the complexes with DBPN, chloride and nitrate salt of cobalt and nickel are measured Conductances at 25℃. The equivalent conductances are affected by the volume of organosolvents added, kinds of organosolvents, solvation, cations and anions, and the stability constants of complexes with Co^2+ and Ni^2+ are in the order of AN>DMSO>TMF>DMF, and the stability constants of these complexes are in the order of Co^2+>Ni^2+ and Cl^->NO_3^-, respectively. The stability constants of these complexes depends highly on relative ratio of cavity size of DBPN and cation size. The λ_max. of these complexes are shifted by solvents used and these complexes are also shifted in the direction of the long wavelength under the aprotic solvents.

Method for Protection of Single‑Line‑Ground Fault of Distribution System with DG Using Distance Relay and Directional Relay

Jung‑Hun Lee,Min‑Su Park,Hong‑Seon Ahn,Kyung‑Won Park,Jun‑Seok Oh,Seung‑Gyu Jeon,Dong‑Kyu Kim,Jae‑Eon Kim 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.4

When large scales DG are connected to the distribution system of unidirectional power currents, bi-directional power currents are formed, making it impossible to use conventional overcurrent protection methods alone to detect and isolate fault sections for ground and short-circuit faults. To solve these problems, this study proposed the method of protection against single-line-ground fault of common neutral line multi-contact local power distribution system using distance relay and directional relay. The proposed protection method was applied to the common neutral multi-ground distribution system to validate the protection against single-line-ground fault through the PSCAD/EMTDC software package and efectively detect and isolate only the zone of single-line-ground fault without malfunctions.

비만증 환자에서 한약제에 의한 체중 감소효과

Oh, Seung-Joon,Jeong, In-Kyung,Kim, Young-Seol,Choi, Young-Kil,Paeng, Jeong-Ryung,Bae, Jung-Hwan,Shin, Hyun-Dae 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Seung-Joon Oh, In-Kyung Jeong, Young-Seol Kim, Young-Kil Choi, Jeong-Ryung Paeng¹, Jung-Hwan Bae and Hyun-Dae Shin²Department of internal Medicine, College Medicine, Endocrine Research Institute¹. Department of Rehabilitation, College of Oriental Medicine², Seoul, korea. Bady Fat Reduction Effects of Red Ginseng Compound Preparation on the Patients with Obesity. Proceedings of International Symposium on East-West Medicine, Seoul. 244-254, 1999.-Obesity can be defined as a metabolic disease due to an increased state of fat tissues caused by an imbalance of calorie intake and use. Recently, in Korea by improvement and westernization of food intake, along with decrease in exercise activities, the prevalence of obesity has increased greatly. Our objectives were to study stability and effects of decrease in body fat by administering red ginseng compound preparation (known to have body fat decreasing effects in laboratory animals) to obesity patients on low calorie diets. Changes in weight and body fat were measured while carrying out calorie-restricted diets on patients for 4 weeks, then administering red ginseng compound preparation for another 4 weeks. The patients were 20 people whose BMI were 25kg/㎡ or over and whose percent body fat was also 30% or over when tested by bioelectrical conductivity. 1. Changes in weight were from 70.04kg(base line) to 67.43kg(after taking red ginseng compound preparation). 2. In similar sense, BMI decreased from 27.12kg/㎡(base line) to 26.56kg/㎡(after dieting), and further to 26.01kg/㎡ (after taking red ginseng compound preparation). The BMI seemed to decrease significantly compared to the baseline after the use of red ginseng compound. 3. Waist hip ratio was changed from 0.8858(base line) to 0.8728, but it was statistically insignificant. 4. The percent body fat was 35,16%(base line), 33.87%(after dieting), and 31.68%(after taking red ginseng compound preparation). 5. Complete blood cell count and blood chemisrty remained unaffected by the administration. 6. In concern to endocrinologic studies, T3 decreased from 118.7 to 98.2ng/dL, and T4 increased from 8.8 to 9.2㎕/ dL. Epinephrine showed a tendency to decreased from 0.27 to 0.25 ng/mL, and norepinephrine increased from 0.39 to 0.44ng/mL. 7. Leptin was not changed. 8. Some patients complained adverse effects; constipation(5 patients, may be due to diet therapy), fatigue (2 patients), pruritus(2 patients), flushing(s patients), dizziness(3 patients) and epigastric discomfort(2 patients). However their symptoms were mild, so medication did not stopped. In conclusion, loss of weight without significant side effects was observed during low calorie diet and red ginseng compound preparation administration. This is thought to be in relation to sympathetic nerve system rather than adrenal gland. Also, further long0term studies should be required, since the observed results were based on short-term changes in weight.

풋마늘의 冬季養液裁培에 關한 硏究

鄭恩周,吳仲烈,黃海鎭,韓相政 대구효성가톨릭대학교 응용과학연구소 1995 응용과학연구논문집 Vol.4 No.-

겨울철 新鮮한 풋마늘의 生産體系 確立으로 端境期를 解消함과 아울러 아파트 人口가 增加하고 있는 오늘날 室內에서 簡單한 方法으로 풋마늘을 生産하는데 必要한 資料를 얻고자 하였다. 1) 萌芽率은 15±1℃區와 20±1℃區의 Hyponex와 水道물에서 瀋陽種(暖地型)이 瀋種 7日後에, 그리고 濟州種(暖地型)이 播種 21日後에 各各 100%를 나타내었다. 그러나 義城種(寒地型)은 15±1℃區에서 播種 7日後에 全혀 萌芽하지 않았고 21日後에 15±1℃區와 20±1℃區에서 各各 85% 및 95%의 萌芽率을 보였다. 2) 草長은 15±1℃區보다 20±1℃區에서, 그리고 水道물보다 Hyponex에서 더 크며 그의 伸長速度가 빨랐다. 瀋陽種은 15±1℃區와 20±1℃區의 Hyponex와 水道물에서 播種 35日後에 잎이 黃化하면서 草長의 伸長이 停止되었으나 濟州種과 義城種은 伸長을 繼績하였다. 瀋陽種은 다른 2品種에 比하여 草長이 顯著히 컸으며 伸長速度가 매우 빨랐다. 3) 播種 21日後의 葉數, 葉?徑, 葉重 및 葉?重은 15±1℃區보다 20±1℃區에서, 그리고 水道물보다 Hyponex에서 더 增加하였다. 供試 3品鍾中 瀋陽種이 各處理區에서 葉數가 顯著히 많고 葉重이 무서웠다. 義城種은 15±1℃區의 Hyponex와 水道물에서 葉伸長을 볼 수 없었다. 4) 比生長率(CGR)은 15±1℃區와 20±1℃區의 Hyponex와 水道물에서 瀋陽種과 濟州種은 播種7日後에, 그리고 義城種은 播種 14日後에 極大期를 나타낸 다음 漸次 減少하였다. 極大期의 比生長率은 15±1℃區보다 20±1℃區에서, 그리고 水도물보다 Hyponex에서 더 높았다. 5) 20±1℃區에서 CGR의 잎으로서 寄與率은 瀋陽種과 濟州種이 播種21日後까지 增加한 다음 漸次 減少하는 傾向을 보였으나 義城種은 繼績 增加하였고, 뿌리로의 寄與率은 3品種 모두 播種14日後부터 急激히 減少하였다. 6) 葉面積指數는 20±1℃區에서 3品種 모두 水道물보다 Hyponex에서 높았다. 播種28日後의 葉面積指數는 濟州種이 2.0 그리고 義城種이 1.0이었는데 比하여 瀋陽種은 4.0 以上이었다. 7) 本實驗의 어느 境遇에 있어서도 瀋陽種이 濟州種 및 義城種에 比하여 萌芽率가 빠르고 草長이 크고 葉收量이 많았으며, 15±1℃區보다 20±1℃區에서 그리고 水道물보다 Hyponex에서 生育이 良好하였다. 그리고 瀋陽種은 20±1℃區의 Hyponex와 水道물에서 播種後 14日이 經過하면 收穫이 可能하였다. The purpose of this study is to establish a production system for providing fresh leafy garlic in winter season and to obtain data for an easy method of indoor nutriculture of this plant, especially in apartment life. 1. Emergence rates in 15℃ and in 20℃ cultured by Hyponex and by tap water were 100% after 7 and 21 days from planting in Shenyang cultivar, a Southern ecotype, and in Cheju cultivar, a Southern ecotype respectively. Euisung cultivar, a northen ecotype, did not planting uner 15℃ until 7 days from planting, but emerged 85% and 95% after 21 days from planting under 15℃ and 20℃ conditions, respectively. 2. Plant lengths were higher in 20℃ and in Hyponex culture than in 15℃ and in tap water culture, respectively. Shenyang cultivar in 15℃ and 20℃ cultured by Hyponex and tap water ceased to eloongate the leaf and showed chlorosis after 35 days from planting, but Cheju and Euisung cultivars continued to elongate the leaf. The plant length of Shenyang cultivar was especially long as compared with other two cultivars and grew fast. 3. After 21 days from planting, leaf number, diameter of leaf sheath, leaf weight and weight of leaf sheath were higher in 20℃ and in Hyponex culture than in 15℃ and in tap water culture. respecively. Among three cultivars, Shenyang had much more leaves and heavy ones. 4. Comparative crop growth rate(CGR) showed at maximum after 7 days from planting in 15℃ and in 20℃ cultured by Hyponex and by tap water in Shenyang and Cheju cul-tivar while after 14 days from plamting in Euisung cultivar, then decreased gradually afterward. The CGR at maximum was higher in 20℃ and in Hyponex culture than in 15℃ and in tap water cul-ture, respectively. 5. The percentage of contribution of CGR to leaf were increased until 21 days from planting, then decreased gradually afterward in Shenyang and Cheju cultivars, but Euisung showed continu-ous increase. The percentage of contribution of CGR to root declined markedly after 14 days from planting in all three cultivars. 6. Leaf area index(LAI) were high in all three cultivars in 20℃ in Hyponex culture as compared with tap water culture. After 28 days from planting, LAI was 2.0 and 1.0 in Cheju and in Euisung cultivar respectively, comparing with above 4.0 in Shenyang cultivar. 7. In every conditions, Shenyang cultivar exceeded in emerging date, plant length and leaf produc-tion, and the growth was better in 20℃ and in Hyponex culture than in 15℃ and in tap water cul-ture, respectively. After 14 days from planting, Shenyang cultivar was possible to harvest in 20℃ cultured by Hyponex and by tap water.

Enhanced Densification and Hardness of Titanium Bodies Sintered by Advanced Hydrogen Sintering Process

Jung‑Min Oh,Ja‑Geon Koo,Jae‑Won Lim 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.6

A new sintering technique for enhancing a densification and hardness of sintered titanium body by supplying hydrogen wasdeveloped (Hydrogen Sintering Process, HSP). The HSP was developed by only injecting hydrogen into an argon atmosphereduring the core time. As a result, sound titanium sintered bodies with high density and hardness were obtained by the HSP. In addition, a pore size and number of the HSP specimens were smaller than those of the argon atmosphere specimen. It wasfound that the injecting hydrogen into the argon atmosphere by HSP can prevent the formation of oxide layers, resulting inenhanced densification and hardness.

IEEE 802.15.4 MAC/PHY를 이용한 TDMA 기반 Superframe 네트워크 구현

오석호 ( Sukho¸ Oh ),김강묘 ( Kang-myo Kim ),정하연 ( Hayeon Jung ),김기형 ( Ki-hyung Kim ),유승화 ( Seung-wha Yoo ) 한국정보처리학회 2011 한국정보처리학회 학술대회논문집 Vol.18 No.1

산업장 기반 모니터링 시스템에 무선 네트워크 기술의 도입은 시스템 도입 및 유지보수에 있어 반드시 필요하다. 무선 네트워크 기반의 산업장 모니터링을 위해 현재 USN 네트워크에서 광범위하게 사용되고 있는 IEEE 802.15.4 하드웨어 및 MAC 을 이용하여 Freequency Hopping을 수행하는 Superframe 기반의 TDMA 기술을 구현하였다.

Scanning tunneling microscopy investigation of nano-structured α-K5PW11(M x OH2)O39(M = Mn(II), Co(II), Ni(II), and Zn(II)) Keggin heteropolyacid catalyst monolayers.

Choi, Jung Ho,Kang, Tae Hun,Bang, Yongju,Yoo, Jaekyeong,Jun, Jin Oh,Song, In Kyu American Scientific Publishers 2014 Journal of nanoscience and nanotechnology Vol.14 No.11

<P>Nano-structured α-K5PW11(M x OH2)O39 (M = Mn(II), Co(II), Ni(II), and Zn(II)) Keggin heteropolyacids (HPAs) were investigated by scanning tunneling microscopy (STM) and tunneling spectroscopy (TS) measurements in order to elucidate their redox property and oxidation catalysis. HPA molecules formed two-dimensional self-assembled monolayer arrays on highly oriented pyrolytic graphite (HOPG) surface. Furthermore, HPAs exhibited a distinctive current-voltage behavior referred to as negative differential resistance (NDR) phenomenon. The measured NDR peak voltage of HPAs was correlated with the reduction potential and the absorption edge energy determined by electrochemical method and UV-visible spectroscopy, respectively. NDR peak voltage of HPAs appeared at less negative voltage with increasing reduction potential and with decreasing UV-visible absorption edge energy. The correlations strongly suggested that NDR phenomenon was closely related to the redox property of HPAs. Vapor-phase oxidation of benzyl alcohol to benzaldehyde was carried out as a model reaction to track the oxidation catalysis of HPAs. NDR peak voltage appeared at less negative voltage with increasing yield for benzaldehyde.</P>

‘수주산업 회계 투명성 제고방안’의 의도를 시장은 인지하고 있는가?

옥오정(Ohk, Oh-Jung),박종국(Park, Jong-Kook),김수진(Kim, Soo-jin) 한국경영교육학회 2021 경영교육연구 Vol.36 No.3