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Liang-Liang Chen,Ge-Xin Gao,Fei-Xia Shen,Xiong Chen,Xiao-Hua Gong,Wen-Jun Wu 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.9
As the most common type of endocrine malignancy, papillary thyroid cancer (PTC) accounts for 85-90% of all thyroid cancers. In this study, we presented the hypothesis that SDC4 gene silencing could effectively attenuate epithelial mesenchymal transition (EMT), and promote cell apoptosis via the Wnt/β-catenin signaling pathway in human PTC cells. Bioinformatics methods were employed to screen the determined differential expression levels of SDC4 in PTC and adjacent normal samples. PTC tissues and adjacent normal tissues were prepared and their respective levels of SDC4 protein positive expression, in addition to the mRNA and protein levels of SDC4, Wnt/β-catenin signaling pathway, EMT and apoptosis related genes were all detected accordingly. Flow cytometry was applied in order to detect cell cycle entry and apoptosis. Finally, analyses of PTC migration and invasion abilities were assessed by using a Transwell assay and scratch test. In PTC tissues, activated Wnt/β-catenin signaling pathway, increased EMT and repressed cell apoptosis were determined. Moreover, the PTC K1 and TPC-1 cell lines exhibiting the highest SDC4 expression were selected for further experiments. In vitro experiments revealed that SDC4 gene silencing could suppress cell migration, invasion and EMT, while acting to promote the apoptosis of PTC cells by inhibiting the activation of the Wnt/β-catenin signaling pathway. Besides, si-β-catenin was observed to inhibit the promotion of PTC cell migration and invasion caused by SDC4 overexpression. Our study revealed that SDC4 gene silencing represses EMT, and enhances cell apoptosis by suppressing the activation of the Wnt/β-catenin signaling pathway in human PTC.
Liang, Jun,Qian, Ying,Xu, Dan,Yin, Qun,Pan, Hui-Juan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6
Objective: To explore changes in the serum tumor makers, hypoxia-inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$) and vascular endothelial growth factor (VEGF) level and their relations in patients with non-small cell lung cancer (NSCLC) before and after intervention. Materials and Methods: Forty patients with NSCLC and 40 healthy individuals undergoing physical examination in our hospital provided the observation and control groups. HIF-$1{\alpha}$ and VEGF levels in serum were detected by enzyme-linked immuno-sorbent assay (ELISA) in the observation group before and after intervention and in control group on the day of physical examination, along with serum carcino-embryonic antigen (CEA), neuron-speci ic enolase (NSE) and squamous cell carcinoma antigen (SCC) levels in the observation group with a fully automatic biochemical analyzer. Clinical effects and improvement of life quality in the observation group were also evaluated. Results: The total effective rate and improvement of life quality after treatment in observation group were 30.0% and 32.5%, respectively. Serum HIF-$1{\alpha}$ and VEGF levels in the control group were lower than that in observation group (p<0.01), but remarkably elevatedafter intervention (p<0.01). In addition, serum CEA, NSE and SCC levels were apparently lowered by treatment (p<0.01). Serum HIF-$1{\alpha}$ demonstrated a positive relation with VEGF level (p<0.01) and was inversely related with CEA, NSE and SCC levels (p<0.01). Conclusions: Significant correlations exist between marked increase of serum HIF-$1{\alpha}$ and VEGF levels and decrease of indexes related to hematological tumor markers in NSCLC patients after intervention.
Optimization of GEMV on Intel AVX Processor
Jun Liang,Yunquan Zhang 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.2
To improve the performance of BLAS 2 GEMV subroutine under the latest instruction set, Intel AVX, this paper presents a new approach to analyze the new generation instruction set and enhance the efficiency of current data-oriented math subroutines. The whole optimizing process involves memory access optimization, SIMD optimization and parallel optimization. Also, this paper shows the comparison between the traditional SSE instruction set and the AVX instruction set. Experiments show that the optimized GEMV function has obtained considerable increase on performance. Compared with the Intel MKL, GotoBLAS, ATLAS, this optimized GEMV exceeds these BLAS implementations from 5% to 10%.
Thickness effects of aerosol deposited hygroscopic films on ultra-sensitive humidity sensors
Liang, Jun-Ge,Kim, Eun-Seong,Wang, Cong,Cho, Myung-Yeon,Oh, Jong-Min,Kim, Nam-Young Elsevier 2018 Sensors and actuators. B Chemical Vol.265 No.-
<P><B>Abstract</B></P> <P>Hygroscopic film that has a super mesoporous structure exhibits high sensitivity and fast response/recovery in humidity sensing applications. Aerosol deposition (AD) via a shock-loading-solidification preparation mechanism, which is an unexplored potential hygroscopic film preparation technique, can produce a porous microstructure, and the AD hammering effect creates various interior and surface microstructures in different-thickness films. The objective of our study was to verify the feasibility of using AD as a humidity-sensing film preparation technique, and to investigate the thickness effects of the film on its microstructure and hygroscopic properties. Hygroscopic films with thicknesses ranging from 0.1–10.0 μm were aerosol deposited with BaTiO<SUB>3</SUB> powders before undergoing a 400 °C thermal treatment. The resulting surface morphology exhibited roughness increases for thicknesses from 0.1–6.0 μm, and a decrease at 10.0 μm. The cross-sectional structure was characterized by a transitional-density grain distribution in which higher density small-sized grains could be seen in the bottom layer. Films with thicknesses of 1.5 μm and 3.0 μm achieved excellent sensitivities of 178.6 ± 7.3 pF/%RH and 299.9 ± 5.4 pF/%RH, respectively. Further, the response and recovery time for the 1.5 μm film were both less than 5 s. The current study determined the feasibility of AD-based humidity-sensing film preparation and provided a reference for optimal thickness control. The surface hydrophilicity, pore volume, and open-pore ratio were analyzed as critical factors of the thickness related humidity sensing effects, and physical modeling indicated that AD-prepared films exhibited an expanded humidity detection range, enhanced water vapor adsorption and desorption, and improved sensitivity to humidity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Aerosol deposition can be used to prepare ultra-sensitive ceramic humidity sensors. </LI> <LI> Film-based sensors were prepared using a shock-loading solidification mechanism of aerosol deposition. </LI> <LI> Their sensitivity depends on the hydrophilicity, pore volume, and open-pore ratio. </LI> <LI> The thickness of the film regularly affects the humidity-sensing properties. </LI> </UL> </P>
Liang Chen,Hongyuan Fu,Tongyu Lu,Jianye Cai,Wei Liu,Jia Yao,Jinliang Liang,Hui Zhao,Jiebin Zhang,Jun Zheng,Yingcai Zhang,Yang Yang 대한암학회 2020 Cancer Research and Treatment Vol.52 No.2
Purpose Microtubule-associated protein 1 light chain 3B (LC3B) serves as a key component of autophagy, which is associated with the progression of carcinoma. Yet, it is still unclear whether LC3B is also an independent risk factor for intrahepatic cholangiocarcinoma (ICC). We aim to explore the predictive value of LC3B on prognosis of ICC, and to establish a novel and available nomogram to predict relapse-free survival (RFS) and overall survival (OS) for these patients after curative-intent hepatectomy. Materials and Methods From August 2004 to March 2017, 105 ICC patients were eligibly enrolled in the Third Affiliated Hospital of Sun Yat-sen University. Preoperative clinical information of enrolled patients was collected. Expression LC3B in the ICC specimen was detected by immunohistochemistry. Results The 5-year RFS and OS in this cohort were 15.7% and 29.6%, respectively. On multivariate Cox regression analysis, independent risk factors for 5-year OS were cancer antigen 125, microvascular invasion, LC3B expression and lymph node metastasis. Except for the above 4 factors, neutrophil/lymphocyte ratio and tumor differentiation were independent factors for 5-year RFS. The area under the curve of nomograms for OS and RFS were 0.820 and 0.747, respectively. Conclusion The nomograms based on LC3B can be considered as effective models to predict postoperative survival for ICC patients.
( Liang Chen ),( Xiang Zou ),( Ran-xi Zhang ),( Chang-jun Pi ),( Nian Wu ),( Liang-jun Yin ),( Zhong-liang Deng ) 생화학분자생물학회(구 한국생화학분자생물학회) 2016 BMB Reports Vol.49 No.2
Engineered bone tissue is thought to be the ideal alternative for bone grafts in the treatment of related bone diseases. BMP9 has been demonstrated as one of the most osteogenic factors, and enhancement of BMP9-induced osteogenesis will greatly accelerate the development of bone tissue engineering. Here, we investigated the effect of insulin-like growth factor 1 (IGF1) on BMP9-induced osteogenic differentiation, and unveiled a possible molecular mechanism underling this process. We found that IGF1 and BMP9 are both detectable in mesenchymal stem cells (MSCs). Exogenous expression of IGF1 potentiates BMP9-induced alkaline phosphatase (ALP), matrix mineralization, and ectopic bone formation. Similarly, IGF1 enhances BMP9-induced endochondral ossification. Mechanistically, we found that IGF1 increases BMP9-induced activation of BMP/Smad signaling in MSCs. Our findings demonstrate that IGF1 can enhance BMP9-induced osteogenic differentiation in MSCs, and that this effect may be mediated by the enhancement of the BMP/Smad signaling transduction triggered by BMP9. [BMB Reports 2016; 49(2): 122-127]