Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

실용음악 전공 학생들의 발성치료 전후 성대 및 음성 특성 비교

황연신,심미란,김철준,최용석,김상연,최찬호,선동일,Hwang, Yeon Shin,Shim, Mi Ran,Kim, Chul Jun,Choi, Yong Suk,Kim, Sang Yeon,Choi, Chan Ho,Sun, Dong Il 대한후두음성언어의학회 2016 대한후두음성언어의학회지 Vol.27 No.2

Background and Objectives : Many students major in applied music singing have various difficulties in phonation when they sing or perform. But the studies about this cause are lack. The purpose of this study is whether singing voice therapy based on vocal music is effective or not to applied music singing students that have difficulties in phonation. Materials and Methods : Singing voice therapy program had been done to 8 students major in applied music singing during 7 weeks. We did laryngeal stroboscope, perceptual, acoustic and aerodynamic examination before and after singing voice therapy to this students. And we studied post examination results have differences or improvements compared to pre examination results. Results : Four male students don't have effective improvement after singing voice therapy but they shows slight improvement in aerodynamic examination values compared to pre examination values. Four female students shows improvement in aerodynamic examination values and pitch range increasing. Above all, one student's vocal nodules disappeared after singing voice therapy. Conclusion : Singing voice therapy based on music singing is very effective to the students major in applied music singing. Above all, their curriculum in applied music singing should include phonation training based on music singing before training perform and technic practices.

의대생의 스트레스에 영향을 미치는 요인 분석

전진용,김상아,문동석,강동원,박웅섭 관동대학교 의과학연구소 2000 關東醫大學術誌 Vol.4 No.1

Background : Generally, it is well known that medical students have lots of stress because they have many classes of which the courses are much different from those of non-medical students. So, this study was designed for the purpose of evaluating the influencing factors on medical students' stress. Method : This study was carried out with the data of 69 students in premedical course and 66 students in medical course, who answered the survey by Kwandong Medical College, Department of Preventive Medicine and Public Health in June, 1999, The independent variables are the general characteristics, the characteristics related with study, the state of health, the activity for free time. The amount of stress is measured by Korean-translated BEPSI (Brief Encounter Psychosocial Instrument). Result : The multiple recession and logistic analysis show that the grade influence the significant difference of the stress level of the students in premedical course. The entrance age, average selfstudy time, hobby and religion influence the significant difference of the stress level of the students in medical course. The grade, avercage self-studying time and smoking habits influence the signifiant difference of the stress level of the medical students. Conclusion : This result will be used for the management of the stress level of the medical students' that influences their study.

대학야간강좌 개선방안 : 교수들의 견해를 중심으로 The professor's opinion

전성연,이경화,손희권,송선희 고려대학교 교육문제연구소 2001 敎育問題硏究 Vol.15 No.-

본 연구에서는 "야간 대학 강좌의 활성화"를 위한 대안을 마련하기 위하여 전국 야간 강좌 개설 대학의 교수를 대상으로 설문 조사를 실시하였다. 설문에 응한 교수는 총342명이었으며 주요한 조사결과는 다음과 같았다. 첫째, 야간강좌의 목적은 현재 주간자원활용에 초점을 두고 있으나 앞으로는 사회 변화를 적절히 반영하고 사회발전에 기여하는 쪽으로 변화되어야 한다. 둘째, 학생선발은 내신과 수능을 동시에 고려하는 현행제도와 더불어 앞으로 내신만으로 하는 방향에 대한 요구가 높았다. 셋째, 학생활동 및 행정절차에 부정적인 반응이 많았으며 이에 대한 배려가 필요하다. 넷째, 교육과정 운영에 있어서 전공, 교양 과목의 비율이 야간강좌의 특성을 반영하고 있지 못하므로 이에 대한 대책이 필요하다. 다섯째, 시설·설비·후생복지가 많이 미흡하므로 이에 대한 지속적인 관심과 점검이 필요하다. 여섯째, 수업시간과 수업운영이 전일제 혹은 탄력적으로 운영될 것이 요망된다. 마지막으로 대학 야간강좌 교수들의 심리적인 변인들에 대해서는 학생 및 교직원과의 인간적인 관계에 있어서는 만족하나 교수처우문제에서는 만족하고 있지 않은 것으로 나타났다.

自治區財政調整에 관한 硏究

全相京 釜山大學校行政大學院地方行政硏究所 1989 地方과行政硏究 Vol.1 No.2

『 한군평과 그의 시교고』

유성준 한국외국어대학교 외국학종합연구센타 중국연구소 1992 中國硏究 Vol.13 No.-

한강 퇴적물에 함유된 오염물질의 존재형태와 이동성에 관한 연구 : 팔당호 퇴적물의 인과 중금속을 중심으로

전상호 江原大學校 附設 環境硏究所 1990 環境硏究 Vol.7 No.-

Fractional composition and mobility of sediment phosphorus and some heavy metals in Lake Paldang are investigated. The fractional scheme for sediment phosphorus was made for four chemi-cally defined phosphorus forms as adsorbed phosphorus, non. apatite in-organic phosphorus (NAI-P), apatite phosphorus and organic phosphorus (residual P) (Hieltjes and Lijklema, 1980). The most abundant fraction of the sediment phosphorus in NAI-P and secondly organic fraction, thirdly abundant apatite fraction. Adsorbed frac-tion is minor part of the total phosphorus. Mobilization of sediment phoshor-us in Lake Paldang is most likely to occur when the pH increases or oxygen depleted in bottom water. The most abundant fraction of the sediment metal is residual fraction in all metals investigated. Labile fraction of the sediment metals are 18.0% of total Cu, 51.5% of total Zn, 45.3% of total Pb, and 18.8% of total Ni respectively. Most of labile fraction consists of carbonate fraction for Cu, reducible frac-tion for Zn, Pb and Ni. Mobilization of Cu is most likely to occur when pH decreasesm and Zn, Pb and Ni when oxygen is depleted.

社會福祉政策의 분석 : with respect to medical security in Korea 醫療保障制度를 中心으로

全相京 東亞大學校 大學院 1983 大學院論文集 Vol.7 No.-

Much emphasis had been laid upon social development since the fourth Five-Year Economic Plan. Along with this move to put emphasis upon social development, we are more concerned with social welfare than ever before. There are two views on social welfare: residual conception and institutional one. The residual conception of welfare lends weight to the conception that social welfare serves mainly such function wherein its activities are perceived as necessary only when those who are served by the "normal" institutional channels are unable to benefit, either through personel failings and exceptional needs or when those "normal" channels fail to perform appropriately. On the other hand, the institutional conception of welfare is as a distinct pattern of activities serving not a safety net to catch the pieces after all else has failed, but as an integral and "normal first line function of modern industrial society." Medical security is one of social welfare policies in functional views. And medical secutity consists of medical care and medical insurance. Medical care is a kind of public assistance which is conducted by the government revenue, while medical insurance is a sort of social insurance which aims the dispersion of social risk and income re-distribution. When we say about medical security from here on, we mean it both medical care and medical insurance. We analyze medical security using Gilbert and Specht's framework of social welfare policy. Their framework are as follows: the bases of eligibility of medical security benefits, the kind of medical security benefits, the mode of financing for medical security benefits and the delivery system of medical security benefits. To improve the relevance of this study, we should conduct field survey to distil useful information from the receipients of this policy, the authorities concerned with this policy and medical centers which deliver medical services to recipients. But due to many constraints, we depend upon only literary survey such as the acts concerned with medical security.

기술집약적 시장에서의 수직적 브랜드확장 시 확장제품의 광고유형에 따른 소비자의 확장제품 평가에 관한 연구 : 지각된 적합성과 브랜드확장 방향의 조절효과

전성률,허종호 서강대학교 경영연구소 2005 서강경영논총 Vol.16 No.1

본 연구에서는 확장제품의 광고유형(정교화 광고 vs. 관련성 광고)에 따른 효과를 수평적 브랜드확장(horizontal brand extension) 상황에서의 모브랜드와 확장제품간의 지각된 적합성(perceived fit)만으로 연구범위를 한정시켰던 기존 연구에서 더 나아가 제품군간의 상대적 기술수준 차이가 존재하는 기술집약적 시장에서의 수직적 브랜드확장(vertical brand extension)시 확장제품의 광고유형 효과가 모브랜드와 확장제품간의 지각된 적합성과 브랜드확장 방향간의 상호작용에 따라 어떻게 달라지는지 알아보았다. 이를 위해 2(확장제품의 광고유형: 정교화 광고 vs. 관련성 광고) X 2(지각된 적합성 정도: 높음 vs. 낮음) X 2(수직적 브랜드확장 방향: 하향적 vs. 상향적) 집단간 실험설계(between-subjects factorial design)를 적용하여 각각의 실험상황에 있어서 정교화 광고와 관련성 광고 중 어떤 광고전략을 실행하는 것이 확장제품에 대한 소비자의 평가를 높이는데 효과적인지를 살펴보았다. 분석결과, 모브랜드와 지각된 적합성이 낮은 제품으로 하향적 브랜드확장이 이루어진 경우나 모브랜드와 지각된 적합성이 높은 또는 낮은 제품으로 상향적 브랜드확장이 이루어진 경우에는 기존의 수평적 브랜드확장에서와 마찬가지로 정교화 광고가 확장제품에 대한 소비자의 평가를 높이는데 있어서 보다 효과적인 것으로 나타났다. 반면에 지각된 적합성 정도가 높은 제품으로 하향적 브랜드확장이 이루어진 경우에는 기존의 연구에서 확장제품에 대한 광고유형으로 정교화 광고가 효과적인 것으로 단정지었던 것과는 달리, 오히려 관련성 광고가 확장제품에 대한 소비자의 평가를 높이는데 있어서 더욱 효과적인 것으로 나타났다.

대전지역 다환방향족탄화수소(PAHs) 농도 분포 특성

이성회․전준민 한국교통대학교 2019 한국교통대학교 논문집 Vol.54 No.-

Considering the importance of polycyclic aromatic hydrocarbons (PAHs), this study identifies PAHs pollution levels in Daejeon urban areas and human harm levels for major PAHs to obtain necessary data for the direction of management policies and the planning of management. According to the analysis, it was the highest in the area near the industrial complex, Daehwa-dong, and low concentration in Jeongnim-dong, where ordinary housing and small businesses are in a lot. Therefore, it is deemed that there will be a number of industries that emit high concentrations of PAHs in the industrial complex. Also, if you look at each season, the difference in concentration between two seasons in fall and winter, when the concentration is high, there is less in Daehwa-dong near the industrial complex, and in Seongnam-dong, in the downtown area, winter is about four times higher than in fall. Therefore, compared to Seongnam-dong, where concentration is high only at certain times, Daehwa-dong can be seen as an area affected by high concentration for a longer period of time. Overall it is considered to be an area that requires continuous monitoring and management as the concentration is clearly high at the point of the dialog near the industrial complex