Silencing of long noncoding RNA HOXA11-AS inhibits the Wnt signaling pathway via the upregulation of HOXA11 and thereby inhibits the proliferation, invasion, and self-renewal of hepatocellular carcinoma stem cells

Jun-Cheng Guo,Yi-Jun Yang,Jin-Fang Zheng,Jian-Quan Zhang,Min Guo,Xiang Yang,Xiang-Ling Jiang,Li Xiang,You Li,Huang Ping,Liu Zhuo 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Hepatocellular carcinoma (HCC) is a major cause of cancer-related deaths, but its molecular mechanisms are not yet well characterized. Long noncoding RNAs (lncRNAs) play crucial roles in tumorigenesis, including that of HCC. However, the role of homeobox A11 antisense (HOXA11-AS) in determining HCC stem cell characteristics remains to be explained; hence, this study aimed to investigate the effects of HOXA11-AS on HCC stem cell characteristics. Initially, the expression patterns of HOXA11-AS and HOXA11 in HCC tissues, cells, and stem cells were determined. HCC stem cells, successfully sorted from Hep3B and Huh7 cells, were transfected with short hairpin or overexpression plasmids for HOXA11-AS or HOXA11 overexpression and depletion, with an aim to study the influences of these mediators on the self-renewal, proliferation, migration, and tumorigenicity of HCC stem cells in vivo. Additionally, the potential relationship and the regulatory mechanisms that link HOXA11-AS, HOXA11, and the Wnt signaling pathway were explored through treatment with Dickkopf-1 (a Wnt signaling pathway inhibitor). HCC stem cells showed high expression of HOXA11-AS and low expression of HOXA11. Both HOXA11-AS silencing and HOXA11 overexpression suppressed the self-renewal, proliferation, migration, and tumorigenicity of HCC stem cells in vivo, as evidenced by the decreased expression of cancer stem cell surface markers (CD133 and CD44) and stemness-related transcription factors (Nanog, Sox2, and Oct4). Moreover, silencing HOXA11-AS inactivated the Wnt signaling pathway by decreasing the methylation level of the HOXA11 promoter, thereby inhibiting HCC stem cell characteristics. Collectively, this study suggested that HOXA11-AS silencing exerts an antitumor effect, suppressing HCC development via Wnt signaling pathway inactivation by decreasing the methylation level of the HOXA11 promoter.

Neurotrophic Artemin Promotes Motility and Invasiveness of MIA PaCa-2 Pancreatic Cancer Cells

Meng, Ling-Xin,Chi, Yu-Hua,Wang, Xiang-Xu,Ding, Zhao-Jun,Fei, Li-Cong,Zhang, Hong,Mou, Ling,Cui, Wen,Xue, Ying-Jie Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: To analyze the capacity of neurotrophic artemin to promote the motility and invasiveness of MIA PaCa-2 pancreatic cancer cells. Methods: MIA PaCa-2 was cultured in vitro and studied using transwell chambers for motility and invasiveness on treatment with different concentrations of aArtemin or its receptor $GFR{\alpha}3$ were also determined. Expression of matrix metalloproteinase-2 (MMP-2) and epithelial cadherin (E-cadherin) was quantified using RT-PCR and Western blotting. Results: MIA PaCa-2 pancreatic cancer cell motility and invasiveness was significantly increased with artemin and its receptor $GFR{\alpha}3$ with dose dependence (P<0.01). MMP-2 production was also significantly increased (t = 6.35, t = 7.32), while E-cadherin was significantly lowered (t = 4.27, t = 5.61) (P <0.01). Conclusion: Artemin and its receptor $GFR{\alpha}3$ can promote pancreatic cancer cell motility and invasiveness and contribute to aggressive behavior. The mechanism may be related to increased expression of MMP-2 molecule and down-regulation of E-cadherin expression.

Study on the Magnetic Rate Sensitivity and Shear Properties of Magnetorheological Shear Thickening Fluid

Guo-Jun Yu,Shao-Jie Zhu,Cheng-Bin Du,Ling-Yun Wang,Jun-Chi Huang 한국자기학회 2023 Journal of Magnetics Vol.28 No.3

This paper studies magnetic field control and velocity-activated magnetorheological shear thickening fluid (MR-STF). High-concentration STF is composed of nano-sized silica particles suspended in a solvent polyethylene glycol (PEG), and then micron-sized carbonyl iron particles of different mass fractions are added to the STF to manufacture different MR-STF. The rheometer is used to study the viscoelasticity of all four samples. The correlation between dynamic behavior and shear rate, angular frequency, and external magnetic field is studied and discussed. In the lower angular frequency range, the loss modulus is slightly larger than the storage modulus, and MR-STF behaves as a viscoelastic state. After the critical angular frequency, the storage modulus decreases sharply, well below the loss modulus. MR-STF appears in a viscous state and a liquid state. With the start of external field excitation, MR-STF is more inclined to MRF. Finally, the apparent viscosity and shear rate of MR-STF are fitted. The results show that with the increase of magnetic induction strength, the plastic viscosity coefficient of MR fluid increases, the flow characteristic index decreases, and the shear thinning effect becomes more significant.

Hashing via Efficient Addictive Kernel for Logistics Image Classification

Xiao-jun Liu,Qiu-ling Li,Bin Zhang,Jun-yi Li 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.2

In this paper, fast image search with efficient additive kernels and kernel locality-sensitive hashing has been proposed. As to hold the kernel functions, recent work has probed methods to create locality-sensitive hashing , which guarantee our approach’s linear time, however existing methods still do not solve the problem of locality-sensitive hashing (LSH) and indirectly sacrifice the loss in accuracy of search results in order to allow fast queries. To improve the search accuracy, we show how to apply explicit feature maps into the homogeneous kernels, which help in feature transformation and combine it with kernel locality-sensitive hashing. We prove our method on several large datasets, and illustrate that it improve the accuracy relative to commonly used methods and make the task of object classification, content-based retrieval more fast and accurate.

Weighted Centroid Localization Algorithm Based on Mobile Anchor Node for Wireless Sensor Networks

Ma, Jun-Ling,Lee, Jung-Hyun,Rim, Kee-Wook,Han, Seung-Jin Korea Spatial Information System Society 2009 한국공간정보시스템학회 논문지 Vol.11 No.2

Localization of nodes is a key technology for application of wireless sensor network. Having a GPS receiver on every sensor node is costly. In the past, several approaches, including range-based and range-free, have been proposed to calculate positions for randomly deployed sensor nodes. Most of them use some special nodes, called anchor nodes, which are assumed to know their own locations. Other sensors compute their locations based on the information provided by these anchor nodes. This paper uses a single mobile anchor node to move in the sensing field and broadcast its current position periodically. We provide a weighted centroid localization algorithm that uses coefficients, which are decided by the influence of mobile anchor node to unknown nodes, to prompt localization accuracy. We also suggest a criterion which is used to select mobile anchor node which involve in computing the position of nodes for improving localization accuracy. Weighted centroid localization algorithm is simple, and no communication is needed while locating. The localization accuracy of weighted centroid localization algorithm is better than maximum likelihood estimation which is used very often. It can be applied to many applications.

Analysis of Patients’ Consent Right in a Surgery -Triggered from the social event that a parturient in Yulin jumped off a tall building-

Hou, Jun-Ling 중국지역학회 2019 중국지역연구 Vol.6 No.1

The incident that a parturient jumped off a window of a hospital raised people’s reconsideration regarding the assignment of Patient Surgery Consent Right. Since the establishment of People’s Republic of China, this thought experienced three development phases: Administrative Instructions, Administrative Legislation, and the Legislation of National People’s Congress. Based on the consent of family members or interested parties, the right of consent mode, is violating not only the Patient’s right of free choice and informed consent, but the right of doctor’s special handling right. The author believes under non-emergency circumstance and the same kinship sequence, the Consent Right shall be assigned by the rule of rational reasoning, which presumed from blood relationship to the closeness of kinship and to closeness relation in actual life. In addition, author believes the right assignment shall be placed with the perspective of family members, interested parties and doctor’s special disposition, the law shall be amended from above mentioned aspects accordingly.

Learning with Information Entropy Method for Transportation Image Retrieval

Liu Xiao-jun,Li Qing-ling,Li Yong-jian,Li Jun-yi 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.7

As a new learning framework, Multi-Instance learning is labeled recently and has successfully found application in vision classification. A novel Multi-instance bag generating method is presented in this paper on basis of Gaussian Mixed Model. The generated GMM model composes not only color but also the locally stable unchangeable components. It is frequently named as MI bag by researchers. Besides, another method called Agglomerative Information Bottleneck clustering is applied to replace the MIL problem with the help of single-instance learning ones. Meanwhile, single-instance classifiers are employed for classification. Finally, ensemble learning is adopted to strengthen classifiers’ generalization ability of RBM (Restricted Boltzmann Machine) as the base classifier. On the basis of large-scale datasets, this method is tested and the corresponding result shows that our method provides high accuracy and good performance for image annotation, feature matching and example-based object-classification.

도시 공공 공간에서 인터랙티브 미디어 아트 활용 사례 분석

장군령(Zhang jun-ling),김세화(Kim se-hwa) 한국콘텐츠학회 2007 한국콘텐츠학회 종합학술대회 논문집 Vol.5 No.2_2

도시의 발전에 따라 상징적인 건축물 및 대형 광장, 차없는 거리 등이 도시의 중요한 특징으로 부각되고 있으며 이와 함께 공공 예술에 대한 중요성이 부각되고 있다. 도시의 공공 공간을 중심으로 인터랙티브 미디어 아트활동을 하는 9명의 세계적인 작가 또는 그룹을 선정하고, 이들의 대표적인 작품을 대상으로 홍미유발. 인터랙션, 장소의존성, 심미성, 상업성, 매체, 장비의 7개 항목을 기준으로 현대 공공 공간의 인터랙티브 미디어 아트의 특징을 분석하도록 한다. As the development of the city, Emphasis the important characteristic of the such city which has the symbolic building and the diverse squares, In addition, Emphasis the Importance of the public art, Designated 9 examples which Media Artist and Design team who have the world famous, designed Interactive Media Art which applies flexibly in city public space. In view of these representation works, make the 7 Projects: Object, Interest enticement, Interactive, Place regional characteristic, Esthetics characteristic, Industry, Medium, Equipment as the datum, to analysis the Interactive media art characteristic of the modem public space.

The inhibitory effect of pyrogallol on tyrosinase activity and structure: Integration study of inhibition kinetics with molecular dynamics simulation

Xiong, Shang-Ling,Lim, Gyu Tae,Yin, Shang-Jun,Lee, Jinhyuk,Si, Yue-Xiu,Yang, Jun-Mo,Park, Yong-Doo,Qian, Guo-Ying Elsevier 2019 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.121 No.-

<P><B>Abstract</B></P> <P>Pyrogallol is naturally found in aquatic plant and has been proposed as a substrate of tyrosinase. In this study, we evaluated the dual effect of pyrogallol on tyrosinase as an inhibitor in the presence of L‑DOPA simultaneously via integrating methods of enzyme kinetics and computational molecular dynamics (MD) simulations. Pyrogallol was found to be a reversible inhibitor of tyrosinase in the presence of L‑DOPA and its induced mechanism was the parabolic non-competitive inhibition type (<I>IC</I> <SUB>50</SUB> = 0.772 ± 0.003 mM and <I>K</I> <SUB>i</SUB> = 0.529 ± 0.022 mM). Kinetic measurements by real-time interval assay showed that pyrogallol induced rapid inactivation process composing with slight activations at the low dose. Spectrofluorimetry studies showed that pyrogallol mainly induced regional changes in the active site of tyrosinase accompanying with hydrophobic disruption at high dose. The computational MD simulations further revealed that pyrogallol could interact with several residues near the tyrosinase active site pocket such as HIS61, HIS85, HIS259, ASN260, HIS263, VAL283, and ALA296. Our study provides insight into the mechanism by which hydroxyl group composing pyrogallol inhibit tyrosinase and pyrogallol is a potential natural anti-pigmentation agent.</P>

Molecular Mechanism of Plant Growth Promotion and Induced Systemic Resistance to Tobacco Mosaic Virus by Bacillus spp.

Shuai Wang,Hui Jun Wu,Jun Qing Qiao,Ling Li Ma,Jun Liu,Yan Fei Xia,Xue Wen Gao 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.10