Heme oxygenase-1 (HO-1)/carbon monoxide (CO) axis suppresses RANKL-induced osteoclastic differentiation by inhibiting redox-sensitive NF-κB activation

( Sun-uk Bak ),( Suji Kim ),( Hae-jun Hwang ),( Jung-a Yun ),( Wan-sung Kim ),( Moo-ho Won ),( Ji-yoon Kim ),( Kwon-soo Ha ),( Young-guen Kwon ),( Young-myeong Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.2

Heme oxygenase (HO-1) catalyzes heme to carbon monoxide (CO), biliverdin/bilirubin, and iron and is known to prevent the pathogenesis of several human diseases. We assessed the beneficial effect of heme degradation products on osteoclastogenesis induced by receptor activator of NF-κB ligand (RANKL). Treatment of RAW264.7 cells with CORM-2 (a CO donor) and bilirubin, but not with iron, decreased RANKLinduced osteoclastogenesis, with CORM-2 having a more potent anti-osteogenic effect. CORM-2 also inhibited RANKLinduced osteoclastogenesis and osteoclastic resorption activity in marrow-derived macrophages. Treatment with hemin, a HO-1 inducer, strongly inhibited RANKL-induced osteoclastogenesis in wild-type macrophages, but was ineffective in HO-1^+/-cells. CORM-2 reduced RANKL-induced NFATc1 expression by inhibiting IKK-dependent NF-κB activation and reactive oxygen species production. These results suggest that CO potently inhibits RANKL-induced osteoclastogenesis by inhibiting redox-sensitive NF-κB-mediated NFATc1 expression. Our findings indicate that HO-1/CO can act as an antiresorption agent and reduce bone loss by blocking osteoclast differentiation. [BMB Reports 2017; 50(2): 103-108]

Numerical Analysis of Radio‑Frequency Inductively Coupled Plasma Spheroidization of Titanium Metal Powder Under Single Particle and Dense Loading Conditions

Jun‑Seok Nam,Eonbyeong Park,Jun‑Ho Seo 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.4

In order to allow more practical prediction of RF (radio-frequency)–ICP (inductively coupled plasma) spheroidizationresults of titanium metal powder, numerical analyses under single particle and dense loading conditions were carried outand the results were compared. First, both of the numerical results for Ar inductively coupled plasma with the power levelof 30 kW revealed that the injected particles can experience not only spheroidization by melting, but also size reduction byevaporation. In addition, this size reduction was found to strongly depend on the initial sizes of the injected particles, due tothe relatively short heating time. For example, the 100 μm Ti particles were computed to hardly experience size reduction byevaporation regardless of feeding rates. However, relatively small Ti particles < 100 μm can be rapidly heated up to boilingpoints during the short flight of plasma, resulting in the size reduction by the surface evaporation. In particular, numericalresults under dense loading condition showed that the final sizes of these small Ti particles can be changed depending onthe feeding rate. For example, a single 60 μm Ti particle was calculated to be a 51 μm spherical Ti particle due to the excessiveheating. However, with the increase of feeding rate up to 1.0 kg/h, the final sizes of the as-treated Ti powder couldbe improved to 55 μm due to the plasma temperatures decreasing through complicated plasma–particle interactions. Bypredicting the relationships between the feeding rates and the initial diameters of Ti powders at a given plasma power level,numerical modellings under single particle and dense loading conditions can help in optimizing the RF–ICP spheroidizationprocess of titanium metal powder.

정보통합에 의한 동사적 동명사구 연구

조준호 대한언어학회 1997 언어학 Vol.5 No.1

Cho, Jun-Ho. 1997. A Study on Information Unification in VGerPs. Linguistics, 5-1, 85-98. The puropse of this paper is to explain consistently the noun and verbal properties English verbal gerund phrases(VGerPs) have, as used by multiple inheritance hierarchies, constraints, and lexical structures of HPSG Theory. The paper will suggest lexical structures of gerunds to solve the problems the gerunds have. By the same index, the unification of infomation between lexical words and phrases is a way to light the explanation of verbal property and noun property. The way also has a merit to catch and explain an unexpressed subject, PRO. (Hanlyo San Up University)

ERK1/2 activation in quercetin-treated BEAS-2B cell plays a role in Nrf2-driven HO-1 expression

이윤진,Jun-Hwan Song,Myung-Ho Oh,Yong-Jin Lee,김용배,Jae-Hyuk Im,이상한 대한독성 유전단백체 학회 2011 Molecular & cellular toxicology Vol.7 No.4

Quercetin, a member of the flavonoids,has been heralded as chemopreventive agent, generally because of its free radical-scavenging and antiproliferative activities. In this process, NF-E2-related factor-2 (Nrf2) plays a role in protecting cells from oxidative damage; however, the exact mechanisms in which bronchial epithelial cells respond to quercetin by activating Nrf2 are not fully understood. Here, we report the role of extracellular signal-regulated kinase (ERK) activation in response to quercetin. Quercetin treatment decreased the viability of human bronchial epithelial BEAS-2B cells in a dose- and time-dependent manner. ERK1/2 was activated within 10 min of quercetin addition and then remained increased above control level throughout 48 h, whereas AKT phosphorylation was suppressed from the first 1 h of quercetin treatment. Inhibition of Erk1/2 phosphorylation attenuated quercetin-induced loss of cell viability. Nrf2levels in both nuclear and whole cell lysates increased after quercetin treatment and was accompanied by the increase of heme oxygenase-1 (HO-1) protein level,indicating that increased HO-1 expression is Nrf2-mediated. Activation of the Nrf2/HO-1 system after quercetin treatment was suppressed by the pretreatment with MEK inhibitor, PD98059. Also, silencing Nrf2 with siRNA decreased cell viability and augmented cytotoxic effect of anticancer drug LBH589. Overall, our results indicate that quercetin induces the up-regulation of Nrf2-mediated HO-1 expression, at least in part, via MEK/ERK1/2 signaling, as an adaptive mechanism which may provide advantages for cell proliferation and enhance survival despite endogenous and/or exogenous oxidative insults.

홉스의 『시민론』과 『인간론』을 중심으로 본 인권과 전쟁의 권리

이준호 ( Jun-ho Lee ) 서양근대철학회 2018 근대철학 Vol.12 No.-

이 글은 고 이준호 박사의 유고논문이다. 저자가 세운 이 글의 목적은 분명하다. 홉스 연구자들이 『리바이어던』을 main text로 삼고 있는 것에서 벗어나 『시민론』과 『인간론』의 중요성에 주목하고 있다. 필자는 홉스의 시민론을 직접 우리말로 번역한 바 있다. 이 번역 작업을 통해 시민론에 대한 깊은 이해와 성찰을 바탕으로 홉스 정치철학을 새롭게 해석할 가능성을 모색하고 있다. 그가 주목한 새로운 해석이란, 『시민론』에서 홉스는 전쟁을 적극적으로 반대하고 있다는 사실과 신민들의 인권 보호와 복지 증진을 위해 군주가 져야할 자연적 의무가 있다는 것을 보여주는 일이다. 또 최고통치권자에게 복종해야만 하는 근거는 통치권자 자신이 소유한 힘에 대한 ‘공포’에 있지 않고, 실정법을 위반했을 때 받게 되는 처벌의 공포에 무게 중심을 두고 있다. 3절에서 필자는 국가의 두 기원, 동의와 정복에 관해 분석하고 있다. 이 절에서 필자는 실질적 사회계약론에 대해 의문을 제기하고 있다. 애초 국가는 자연발생적으로 시작되었으며, 최고 통치권자의 자의적 통치를 견제하기 위해 신민들과 계약을 맺게 된 것이라는 필자의 해석이다. 4절에서 필자는 시민론의 열 번째 자연법에 주목하고 있다. 공정성, 즉 차별하지 말라는 자연법의 명령은 국가의 평화와 안위를 위해서 통치권자가 지켜야 할 덕목이라는 것이다. 특히 분배의 공정성을 유지하는 일이 통치권자의 의무이다. 5절은 통치권력의 절대성과 복종에 대해 설명하고 있다. 정당화된 권력은 국가의 안전을 위해 신민들의 사생활까지 감시할 수 있어야 한다고 주장한다. 당연히 오늘날의 시각에서 보면 인권침해의 위험이 있지만 홉스 당시에는 문제가 되지 않는다. 그러나 홉스는 부당한 권력에 대해서는 자발적 복종의 의무를 배제하고 있다. 통치권자도 양심과 자연법에 따라 통치할 때만 권력은 정당화되며, 신민들의 자발적 복종을 얻을 수 있다. 6절에서 필자는 자연법을 위반한 통치권자에 대한 전쟁의 권리에 대해 설명하고 있다. 신민을 적으로 만든 통치권자는 그 권력의 정당성을 상실하게 되고, 신민들은 통치권자를 향해 전쟁의 권리를 회복하게 된다는 것이다. 챨스 1세의 처형이 이에 해당된다. 결론에서는 홉스의 정치철학사적 위치를 다시 규정하고 있다. 홉스는 절대왕권주의를 지지하면서도 주권재민론의 선구자로서의 자리매김을 하고 있다. This is a paper by Dr. Lee Jun-ho who died a few years ago. The purpose of this article is clear. Dr. Lee suggests that Hobbes researchers should pay attention to the importance of De Cive and De Homine, not to consider Leviathan as the main work of Hobbes. He translated Hobbes’ De Cive and De Homine into Korean. Through this translation work, he was looking for a possibility to interpret Hobbes’ political philosophy based on deep understanding and reflection on civil theory. The new interpretation he notices is that Hobbes is actively opposed to war in the De Cive and shows that there is a natural obligation of the monarch to protect the human rights and welfare of the people. The author emphasizes that the reason for submitting to the supreme ruler is not the “fear” of the powers held by the ruling power, but the fear of punishment in violation of the law. In section 3, the author analyzes the origins of consent and conquest. In this section, he questions the actual social contract theory. It is his interpretation that the country originally started spontaneously and that it had entered into a contract with the people to check the arbitrary rule of the supreme ruler. In section 5, the author explains the absoluteness and obedience of ruling power. He maintains that justified power has to monitor the privacy of the people for the sake of national security. Of course, from today’s point of view there is a risk of human rights violations, but it is not a problem at the time. However, Hobbes excludes the obligation of voluntary obedience to unjust power. Only when the ruling power is governed by conscience and natural law, power is justified and the voluntary obedience of the people can be obtained. In section 6, the author explains the right of war to the rulers who violate natural law. The rulers who have made the enemies of the people lose the legitimacy of their power, and the people will regain the right of war to the rulers. This is the execution of Charles I. In conclusion, the author redefines the historical location of Hobbes’s political philosophy. According to the author, Hobbes has always been a pioneer of sovereignty, while supporting absolutism.

양극성 장애의 우세삽화와 임상양상 간의 연관성 : 예비연구

서호준,우영섭,채정호,전태연,김광수,박원명 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objective : This study was performed to fmd clinical implications ofdistinctive pattems in bipolar patients with predominance of depressive episodes or manic episodes and to find possibilities to predict predominant episodes in bipolar disorder. Methods : The recmitment period was between 2002 and 2004, among inpatients who had received psychiatric treatment for bipolar disorder. The data about the number and the polarity of the past episodes were collected through medical records, and Sub-jects were divided into depressive or manic episode predominant groups. The two groups were compared regarding Sociodemo-graphic and clinical variables. Results : 74 patients met the inclusion criteria during the study period. 21 subjects were classified as depressive episode Pre-dominant group (depressive episode group), and 53 subjects as manic episode predominant group (manic episode group). There were no differences in sociodemographic data, the number of total episodes and hypomanic episodes, and onset age. However, 90.5% (n=19) subjects of depressive episode group showed depressive episode as first episode, and 88.7% (n=47) of manicepisode group experienced manic episode as first episode (χ²=42.13, df=1, P<0.00l). Psychotic symptomswere significantly more prominent in the manic episode group (χ²=6.85, df=1, P=0.009), and the number ofsubjects who had suicide attempt experience was higher in the depressive episode group (χ²=7.39, df=1, P=0.007). In the comparison of highest GAF scores during the past year, significant difference was found between the two group (p=0.046), as the mean scores of depressive epi-sode group was 68.337±7.47 and manic episode groupwas 71.51±5.42. Conclusion : The results ofthis study show that there are differences in clinical features according to predominant episodes in bipolar disorder. The type of onset episode appears to be related to predominant episode in this study, and it would be a valid Pa-rameter with therapeutic implication in bipolar patients.

전직 관료 영입이 기업성과에 미치는 영향: 산업에 따른 효과성 분석

박준호(Park, Jun-Ho),이장우(Lee, Jang-Woo) 한국경영교육학회 2021 경영교육연구 Vol.36 No.1

[연구목적] 지금껏 전직 관료 영입과 기업성과 간의 관계를 다룬 다양한 연구가 진행되어 왔다. 하지만, 선행연구들은 서로 다른 연구방법과 연구대상으로 인해 혼재된 결과를 보임으로써 지속적인 의문을 증폭시키고 있다. 이에 본 연구는 두 가지 산업을 비교 분석함으로써 전직 관료 영입과 기업성과 간의 보다 명확한 관계를 관찰하고자 하였다. [연구방법] 연구를 위해 1998년부터 2017년까지 총20년의 연구기간을 선정하고 의약품 및 의료기기 제조업 189개 기업과 화학산업에 포함된 화학물질 및 화학제품 제조업 136개 기업의 데이터를 수집하여 연구를 진행하였다. [연구결과] 연구결과, 취약한 제도적 환경으로 평가되는 의료산업에서는 전직 관료 영입이 기업성과에 정(+)의 영향을 미치는 것으로 관찰되었으며 비교적 안정적인 제도 환경으로 평가되는 화학산업에서 전직 관료 영입이 기업성과에 유의미한 관계가 없는 것으로 관찰되었다. [연구의 시사점] 본 연구는 그동안 전직 관료 영입과 기업성과 간의 혼재된 연구결과를 설명하는데 기여할 수 있을 것으로 예상된다. 특히, 기업이 속해 있는 산업의 제도적 환경에 따라 전직 관료 영입이 기업성과에 미치는 영향이 다를 수 있음을 시사하고 있다. [Purpose] So far, various studies have been conducted on the relationship between recruiting former bureaucrats board and corporate performance. However, prior studies have amplified persistent questions by showing mixed results from different methods and subjects. Thus, the study sought to observe a clearer relationship between the two major variables through comparative analysis of two different industries. [Methodology] To fulfill the aim of the research, we covered the corporate performances for 20 years from 1998 to 2017. The data were collected from 189 pharmaceutical/medical companies, and another 136 chemical related companies. [Findings] In the medical industry, which is regarded as a unstable institutional environment, the recruitment of former bureaucrats has been observed to have a positive effect on corporate performance, and in the chemical industry, which is considered to be a relatively stable institutional environment, the recruitment of former officials has no significant relationship to corporate performance. [Implications] The study is expected to contribute to explaining the mixed results of the research between recruiting former bureaucrats and corporate performance. In particular, it suggests that the impact of recruiting former bureaucrats on corporate performance may vary depending on the environment of the industry to which the company belongs.

동명사구의 구조분석

조준호 대한언어학회 1998 언어학 Vol.6 No.1

Cho, Jun-Ho. 1998. Structural Analysis of the Gerundive Phrase. Linguistics, 6-1, 109-129. The purpose of this paper is to explain the structure of English verbal gerundive phrases, with Head-Adjunct Constraint and Reduced Relative Clause within HPSG. On the ground of this approach the paper will examine the relation between VP and its subject in the gerundive phrase. The approach implies the fact that the case and canonical feature the subject has are given as more powerful way to explain the gerundive phrase. (Hanlyo University)

Synthesis and photophysical study of heteroleptic iridium(III) complex

Kim, Jun Ho,Kim, Young Sik Research Institute of Science and Technology HONGI 2005 Hongik Journal of Science and Technology Vol.9 No.-

The synthesis and photophysical study of efficient phosphorescent iridium(III) complexes having two different (CˆN) ligands are reported. In order to improve the luminescence efficiency by avoiding triplettriplet (T-T) annihilation, the iridium complex, Ir(ppy)_(2)(dpq), is designed and prepared where ppy and dpq represent 2-phenylpyridine and 2,4-diphenylquinoline, respectively. Two ppy ligands and a dpq ligand act as a source of energy supply. Since Ir(ppy)_(3) and Ir(dpq)_(3)can be placed in the metal-to-ligand charge transfer (MLCT) excited state, they absorb light effectively. When Ir(ppy)_(2)(dpq) is placed in excited state, the excitation energy is not quenched or deactivated but quickly intramolecular transferred from two ppy ligands to one luminescent dpq ligand. This is because the triplet energy level of ppy is higher than that of dpq and light is emitted from dpq ligand in the end. Thus, Ir(ppy)_(2)(dpq) shows strong photoluminescence from dpq ligand. To analyze luminescent mechanism, we calculated these complexes having two different ligand sets theoretically by using computational method.

Protective Effects of Hyperoside against Carbon Tetrachloride-Induced Liver Damage in Mice

Choi, Jun-Ho,Kim, Dong-Wook,Yun, Nari,Choi, Jae-Sue,Islam, Md. Nurul,Kim, Yeong-Shik,Lee, Sun-Mee American Chemical Society and American Society of 2011 Journal of natural products Vol.74 No.5

<P>In this study, the hepatoprotective effects of hyperoside (<B>1</B>), a flavonoid glycoside isolated from <I>Artemisia capillaris</I>, have been examined against carbon tetrachloride (CCl<SUB>4</SUB>)-induced liver injury. Mice were treated intraperitoneally with vehicle or <B>1</B> (50, 100, and 200 mg·kg<SUP>−1</SUP>) 30 min before and 2 h after CCl<SUB>4</SUB> (20 μL·kg<SUP>−1</SUP>) injection. Levels of serum aminotransferases were increased 24 h after CCl<SUB>4</SUB> injection, and these increases were attenuated by <B>1</B>. Histological analysis showed that <B>1</B> prevented portal inflammation, centrizonal necrosis, and Kupffer cell hyperplasia. Lipid peroxidation was increased and hepatic glutathione content was decreased significantly after CCl<SUB>4</SUB> treatment, and these changes were reduced by administration of <B>1</B>. Protein and mRNA expression of tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and heme oxygenase-1 (HO-1) and nuclear protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2) significantly increased after CCl<SUB>4</SUB> injection. Compound <B>1</B> suppressed TNF-α, iNOS, and COX-2 protein and mRNA expression and augmented HO-1 protein and mRNA expression and Nrf2 nuclear protein expression. These results suggest that <B>1</B> has protective effects against CCl<SUB>4</SUB>-induced acute liver injury, and this protection is likely due to enhancement of the antioxidative defense system and suppression of the inflammatory response.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2011/jnprdf.2011.74.issue-5/np200001x/production/images/medium/np-2011-00001x_0006.gif'></P>