Mycobacteria Growth indicator Tube(MGIT)와 Ogawa 배지를 이용한 결핵균의 신속검출

권오건,조현미,장인호,어영,윤갑준 대한임상미샐물학회 2000 Annals of clinical microbiology Vol.3 No.2

개에 있어서 耳根部의 Lidocaine 水針에 의한 全身 無痛覺의 誘發

권현주,유건주,이상은,이정연,송근호,김명철,정성목,박성준,김덕환 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Twelve clinically healthy mongrel dogs were used to clarify the possibility of general analgesia induction by lidocaine aquapuncture to auricular root area in the present study. The experimental animals were devided into control (6 heads) and experimental groups (6 heads), respectively. Physiological salines (3 ml/kg) were injected into both auricular root areas in control group. Lidocaine hydrochlorides (3 ml/kg) were injected into both auricular root areas in experimental group. Superficial pain, the changes of vital signs and blood leukocytes, and PCV were examined on pre, 5 min., 15 min., 30 min, and 60 min. after injection in control and experimental groups, respectively. Analgesia was not detected at all in control group, however, all experimental animals were recumbent and analgesia was generally detected in the auricles, noses, dorsal parts, ventral areas, limbs and tails from 5 to 30 minutes after injection with lidocaine hydrochloride into auricular root areas in experimental group. As for the change of vital signs, body temperature showed decreasing tendency from 5 minutes after lidocaine injection in experimental group. Heart rates and respiration rates were increased on 5 minutes after lidocaine injection and then they showed decreasing tendency in experimental group. No significant difference were found among groups. As for the changes of hematological findings, the numbers of blood leukocytes were increased till 15 minutes after lidocaine injection and then they showed decreasing tendency. PCV values were increased on 5 minutes after lidocaine injection, and then they showed decreasing tendency. However, no significant differences were observed among groups. Considering the above findings, it was thought that general analgesia was possible by aquapuncture with lidocaine hydrochloride into auricular root areas and lidocaine aquapuncture didn’t affect so much to the changes of vital signs and blood.

임상검체에서 분리된 그람 음성 세균들에 대한 Isepamicin의 시험관내 항균력

권현희,박수진,소민욱,박현구,최성호,김미나,최상호,정진용,우준희,김양수 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background : The increasing incidence of multidrug-resistant gram-negative bacteria causing nosocomial infections is an important clinical problem. Isepamicin is a recently developed aminoglycoside which has been known to have potent activity against gram-negative organisms. We evaluated the in vitro activities of isepamicin and other aminoglycosides against a large number of gram-negative organisms. Materials and Methods : We tested the in vitro antimicrobial activities of isepamicin, amikacin, gentamicin, and tobramycin against 566 gram-negative organisms collected between January 2006 and June 2006 in Asan Medical Center. Minimal inhibitory concentrations (MICs) were determined and interpreted according to the recommendations of Clinical and Laboratory Standard Institute (CLSI). The breakpoint MIC used for interpretation of isepamicin was MIC≤16 ㎍/mL as susceptible, 32 ㎍/mL as intermediate, and 64 ㎍/mL as resistant. Results : The MIC_(50)/MIC_(90) of isepamicln for Escherichia coli, Klebsiella pneumon/ae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Enterobacter cloacae were 1/2, 0.5/>128, 4/16, 16/>128, and 1/2 ㎍/mL, respectively. The susceptibilities for E. coli, K. pneumoniae, P. aeruginosa, A. baumannii, and E. cloacae were 100%, 86.4%, 89.7%, 50.0%, and 96.6%, respectively. For E. coli, K. pneumoniae, P. aeruginosa, and E. cloacae, isepamicln had better in vitro activities than gentamicin and tobromycin, and had similar activities with amlkacin. For A. baumanii, all four tested aminoglycosides had similar in vitro activities. Conclusion : Isepamicin had excellent in vitro activities against gram-negative organisms, except A. baumanii. The overall in vitro activities were similar with amikacin. 목적 : 그람 음성 세균은 다양한 지역사회 및 병원 획득 감염의 원인균이다. 최근에는 병원 감염을 일으키는 그람 음성 세균에서 항균제 내성균의 출현과 확산이 큰 문제가 되고 있다. Isepamicin은 최근에 소개된 gentamicin B의 유도체로 외국에서의 연구에 의하면 그람 음성 세균들에 대한 항균력이 우수함이 보고 된 바 있다. 본 연구에서는 우리나라의 임상 균주들을 대상으로 그람 음성세균에서 isepamicin의 시험관내 항균력을 기존의 아미노배당체 제재들과 비교하여 평가하고자 하였다. 재료 및 방법 : 2006년 1월에서 6월 사이에 서울아산병원 환자에서 분리된 Escherichia coli 113 균주, Klebsiella pneumoniae 110 균주, Pseudomonas aeruginosa 117 균주, Acinetobacter baumannii 108 균주, 그리고 Enterobacter cloacae 118 균주를 대상으로 isepaimcin, amikacin, gentamicin, tobramycin에 대한 최소억제농도(minimal inhibitory concentration, MIC)를 한천 희석법(Mueller-Hinton Agar)을 이용하여 측정하였다. 감수성여부는 CLSI 기준에 따랐으며, isepamicin은 MIC≤16㎍/mL를 감수성, MIC=32 ㎍/mL를 중등도 내성, MIC≥64 ㎍/mL를 내성으로 판정하였다. 결과 : Isepamicm의 E. coli, K. pneumoniae, P. aeruginosa, A baumannii, E. cloacae에 대한 MIC_(50)/MIC_(90)은 각각 1/2, 0.5/>128, 4/16, 16/>128, 1/2 ㎍/mL였고, 항균제 감수성률은 차례대로 100%, 86.4%, 89.7%, 50.0%, 96.6%였다. 기존 아미노배당체 제재들과 비교하면 E. coli, K. pneumoniae, P. aeruginosa, E. cloacae에서는 amikacin과 비슷한 감수성률을 보였고, gentamicin과 tobramycin보다 높은 감수성률을 보였다. A baumannii의 경우 isepamicin의 감수성률이 50.0%로 비교적 낮았지만 amikacin, gentamicin, tobramycin도 각각 50.0%, 46.3%, 47.2%의 감수성률을 나타내어 4가지 항균제가 모두 비슷한 항균력을 보였다. 결론 : Isepamicin은 그람 음성 세균들에 있어 기존의 아미노배당체 제재들과 비교했을 때 amikacin과는 동등한 시험관내 항균력을 보이고 gentamicin, tobramycin보다 뛰어난 시험관내 항균력을 보여 사용이 유망한 아미노배당체 중 하나라 생각된다.

계층화된 퍼지 인지도를 이용한 신뢰기반 경매시스템

김현준,류정섭,권효석,조형래 경상대학교 생산기술연구소 2003 工學硏究院論文集 Vol.19 No.-

This paper introduces trust measurement that is composed of trust variables and fuzzy cognitive map. The improved trust measuring method using a stratified FCM can be used to measure the level of risk involved in a specific auction transaction concerned. To reduce the auction transaction cost and time, the proposed system recommends a differentiated transaction protocol according to the risk based on trust measurement. In this system, trust measurement can also be applied for differentiated and varied auction services.

Mortierella nana의 균체지질중의 γ-Linolenic acid 함량에 미치는 비타민의 영향

박준권,임무현 대구대학교 산업기술연구소 2003 産業技術硏究 Vol.14 No.3

Mortiella nana속의 미생물을 이용한 균체지질중의 GLA생산에서 배양배지내 butyric acid, 수용성 및 지용성 비타민류를 첨가하여 이들 미량물질의 인자가 균의 생육, 지질생산 및 균체지질중의 지방산 변화와 GLA 함량변화에 미치는 영향을 검토하였다. Mortiella nana속의 배양에 각종 수용성비타민을 첨가한 결과, thiamin, riboflavin, niacinamide, pyridoxin의 첨가구에서 균체지질과 GLA함량이 높았다. 수용성 비타민중 thiamin의 농도를 달리하여 첨가한 결과 0.5㎍/㎖의 첨가구에서 지질함량 및 GLA 함량이 높게 나타났다. Ribofalvin, niacinamide, pyridoxin을 각각 농도별로 첨가한 경우 균체지질 함량 면에서 대조구보다 높았으나 GLA함량은 대조구보다 낮게 나타나, 균체지질의 생산에는 유리하게 작용하였지만 GLA의 생산에는 영향을 미치지 못하였다. n-alkane계의 butyric acid를 농도별로 첨가후 배양한 결과 05㎍/㎖의 농도의 첨가구에서 지질생산량은 0.43g이었고 GLA생산량에서는 18.5%로 무첨가구보다 2배이상의 생산량을 보여 균체지질 및 GLA생산에 큰 영향을 주는 것으로 나타났다. 지용성 비타민 중 tocopherol과 squalene을 배지에 첨가후 배양한 결과, 모든 농도의 첨가구에서 지질 생산량 및 GLA생산이 무첨가구보다 낮게 나타났다. 배양배지의 초기 pH의 영향을 조사한 결과 pH 6.0의 배양 처리구가 지질함량 0.45g, GLA생산량이 17.95%로 가장 최적의 초기배양 pH였으며 배지의 양과 배양속도를 달리하여 산소공급의 영향을 조사한 결과, 100rpm에서 150㎖의 배지로 배양한 처리구가 균체지질 및 GLA생산량이 높았으며, 배양 6일째 처리구가 균체 지질 및 GLA 생산성이 가장 높게 나타났다. The effects of water soluble and fat soluble vitamins on the content of γ-linolenic acid(GLA) in the mycelial lipid by culture of Mortiella nana, were investigated. Addition of water soluble vitamins, thiamin, riboflavin niacinamide and pyridoxin, in the culture medium of Mortiella nana resulted in the increase of mycelial lipid and GLA content, In the addition of 0.5㎍/㎖ thiamin to the basal medium, mycelial lipid and GLA content were the highest. In the each addition concentration of riboflavin niacimide and pyridoxin, mycelial content were more higher than control, but GLA content were more lower. It was addition of riboflavin niacinamide and pyridoxin found that mycelial lipid production was favorable, but GLA production was not affected. In the addition of 0.5㎍/㎖ butyric acid as the n-alkane to the basal medium containing 0.5㎍/㎖ thiamin, mycelial content was 0.43g/g dry cell weight and It was found 18.5% of GLA production that addition of 0.5㎍/㎖ was more twice higher than blank. It was appeared that addition of butyric acid was affected to mycelial lipid and GLA production. In the addition of fat soluble vitamin, tocopherol and squalene, mycelial lipid and GLA production were lower than blank at all added concentration. At the influence of intial pH of culture medium, pH 6.0 of culture medium was optimum, the following results were 0.45g of mycelium lipid and 17.95% of GLA production. The influence of oxygen applying with the various of medium volume and two shaking speed were also examed, When cultivation was cultured for 6 days with 150㎖ volume of medium at 100rpm, mycelium lipid and GLA production were the highest.

면역억제제를 사용치 않은 동소성 간이식 실험견의 조직병리소견

조원현,최순옥,김인호,임태진,김성수,박준희,김우석,김현철,최규택,김진모,권건영 大韓移植學會 1994 Korean Journal of Transplantation Vol.8 No.1

관상동맥 스텐트 내 재협착의 발생에 미치는 임상적 관동맥 조영술적 요인들

윤혁준,김기식,박형섭,이영수,현대우,한성욱,허승호,김윤년,김권배,박남희,원경숙 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.12

초음파로 진단된 자궁동정맥 기형 2예

최홍준,이해혁,김태희,김정식,남계현,이권해,박성진 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Arteriovenous fistula of uterus is a very rare cause of massive uterine bleeding. However, it is important to make a rapid and precise diagnosis when it does occur, because life-threatening massive vaginal bleeding may occasionally be a symptom of this disease. Uterine curettage or surgical trauma can cause vascular abnormalities, including pseudoaneurysms, acquired ateriovenous malformations, ateriovenous fistulas, and rupture of vessels. The diagnosis is made by angiography, and gray scale ultrasonography, color and duplex doppler ultrasonography, computed tomography, magnetic resonance imaging is helpful. Ultrasonography is the most commonly performed initial imaging examination for evaluation of abnormal uterine bleeding. Color and duplex Doppler US is an appropriate modality for the detection and diagnosis of uterine ateriovenous malformations and for follow-up after embolization. Transcatheter embolization has replaced hysterectomy as the treatment of choice in women wish to retain thier fertility. A case of arteriovenous malformation of the uterus which was diagnosed by ultrasonography. So reported this case with a brief review of the literature.

상 요추 추간판 탈출증의 수술적 치료

조태현,송준혁,김명현,박향권,김성학,신규만,박동빈 梨花女子大學校 醫科大學 醫科學硏究所 2000 EMJ (Ewha medical journal) Vol.23 No.3

Objectives : Lumbar disc herniations at the L1-L2, L2-L3, and L3-L4 level are rare. The purpose of this study is to observe the variable clinical finding of upper lumbar disc herniations and to provide proper treatment modality. Patients and Methods : Between 1998 Jan. and 1999 Dec., seventeen patients with upper lumbar disc herniations who were undergone surgery in our institute were retrospectively evaluated. Patients were evaluated based on following factors : age, sex, aggravating factor, symptoms and signs type of disc herniation, type of surgery, and outcome of post-surgery. Results : The incidence of lumbar disc herniation at the level of L1-2, L2-3 and L3-4 is 6.1% with declining frequency as the level ascends. The peak age incidence is 6th decade in both sex. Preoperative symptomes and signs are variabe. In sixteen cases, posterior approach was done. In these cases, twelve cases were performed microscopic partial hemilaminectomy, and four cases with spinal stenosis were performed total laminectomy and posterior screw fixation with bone fusion. In one case, lateral extracavitary approach was done. In results of operation, fourteen cases showed more than good grade(82%). Conclusion : The age incidence of lumbar disc herniations at the level of L1-2, L2-3 and L3-4 older than lumbar disc herniations at the lower level of L4-5 and L5-S1. The signs and symptoms are variable. In our cases, most of the patients were performed posterior approach with microscopic partial hemilaminectomy except the patients combined spinal stenosis. One case was performed a lateral extracavitary approach because the risk of the cord and cauda equina injuries. The prognosis of upper lumbar disc herniations after treatment with only microscopic partial hemilaminectomy and diskectomy is comparable with the prognosis of lumbar disc herniations at the lower level.

측두부에 발생한 Cranial Fasciitis의 치험례

정현권,변준희,이종원,이종건,임풍,박성찬 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Cranial fasciitis is a rare morphological variant of nodular fasciitis. It is characterized by a rapid growing fibroblastic proliferative lesion that develops chiefly in childhood. It has varying size and involves the soft tissues of the scalp and the underlying skull. Accurate diagnosis and surgical excision is the key to management. Prognosis is good with rare recurrence. We report a case of cranial fasciitis in the temporal fossa of a 20-month-old girl and present a review of the literature.