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Jumei Wei,Huimin Zhi,Xiaowu Mu 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.12
In this paper, the problem of the E-exponential stability of linear switched singular systems composed of stable and unstable subsystems in discrete-time case is investigated. By using a multiple discontinuous Lyapunov function approach and exploring the properties of mode-dependent average dwell time(MDADT) switching signal, new sufficient conditions of E-exponential stability for linear switched singular systems are presented. Compared with the existing results, the proposed method is more flexible and tighter bounds on MDADT can be obtained. Finally, a numerical example is provided to illustrate the effectiveness of the proposed criteria.
Liwei Chen,Jumei Xu,Weilan Xue,Zuoxiang Zeng 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.1
An eight-step mechanism of esterification reaction between adipic acid (AA) and ethylene glycol (EG) catalyzed by tetrabutyl titanate [Ti(OBu)4] was studied in detail. The kinetic data for the esterification reaction between AA and EG catalyzed by tetrabutyl titanate [Ti(OBu)4] were measured in the temperature range of 403 K-433 K. A second- order kinetic model was established, and the model parameters were obtained through an optimization procedure by minimizing the value differences between the simulated component concentrations in the reaction system with the experimental ones. The results demonstrate that the model is suitable for the esterification reaction between AA and EG catalyzed by tetrabutyl titanate [Ti(OBu)4]. Furthermore, the esterification reaction rate increases with the increase of reaction temperature, concentration of catalyst and the initial reactant ratio of EG to AA.
Xiaoxia Kou,Qingping Wu,Jumei Zhang,Hongying Fan 한국미생물학회 2006 The journal of microbiology Vol.44 No.4
The purpose of this study was to determine the efficacy of a nucleic acid sequence-based amplification (NASBA) method of detecting noroviruses in artificially and naturally contaminated shellfish. We used 58 fecal samples that tested positive for noroviruses with electron microscopy (EM) to develop an NASBA assay for these viruses. Oligonucleotide primers targeting the polymerase coding region were used to amplify the viral RNA in an isothermal process that resulted in the accumulation of RNA amplicons. These amplicons were detected by hybridization with digoxigenin-labeled oligonucleotide probes that were highly specific for genogroup I (GI) and genogroup II (GII) of noroviruses. The expected band of 327 bp appeared in denaturing agarose gel without any nonspecific band. The specific signal for each amplicon was obtained through Northern blotting in many repeats. All fecal samples of which 46 (79.3%) belonged to GII and 12 (20.6%) belonged to GI were positive for noroviruses by EM and by NASBA. Target RNA concentrations as low as 5 pg/ml were detected in fecal specimens using NASBA. When the assay was applied to artificially contaminated shellfish, the sensitivity to nucleic acid was 100 pg/1.5 g shellfish tissue. The potential use of this assay was also confirmed in naturally contaminated shellfish collected from different ponds in Guangzhou city of China, of which 24 (18.76%) out of 128 samples were positive for noroviruses; of these, 19 (79.6%) belonged to GII and 5 (20.4%) belonged to GI. The NASBA assay provided a more rapid and efficient way of detecting noroviruses in fecal samples and demonstrated its potential for detecting noroviruses in food and environmental samples with high specificity and sensitivity.
Real-time PCR Targeting OmpA Gene for Detection of Cronobacter spp. in Powdered Infant Formula
Xiaohui Dong,Qingping Wu,Kui Wu,Jumei Zhang 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.2
Cronobacter spp. (formerly Enterobacter sakazakii), a pathogen commonly found in powdered infant formula (PIF), is a rare cause of invasive infection with a high mortality rate in neonates. In the present study, a realtime PCR assay was conducted to identify the pathogens in PIF using a TaqMan probe targeting the outer membrane protein A gene (ompA) of Cronobacter spp. The specificity of the PCR assay was tested against 25 strains of Cronobacter spp. and 38 non-Cronobacter bacterial species. The detection limits of this method are 1.0×102 copy/μL in standard plasmid, 1.1 CFU/100 g in PIF through 38 h of enrichment,and 2.8×102 CFU/mL in phosphate-buffered saline (pH 7.0). Based on the detection limits, real-time PCR is more sensitive than simplex and duplex PCR. These methods were successfully applied to actual samples, indicating that this real-time PCR assay can be used for the detection of Cronobacter spp. in PIF.
Yang, Xiaojuan,Wu, Qingping,Zhang, Jumei,Guo, Weipeng,Mo, Shuping,Liu, Shengrong 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4
This study aimed to classify a collection of Enterobacter sakazakii (E. sakazakii) strains previously identified from powdered infant formula (PIF) to species level by recN gene sequencing and biochemical testing to determine the distribution of Cronobacter species in China and investigate the strain diversity by cellular fatty acid (CFA) analysis. Of 24 E. sakazakii isolates, 23 were identified as C. sakazakii and one was C. malonaticus. The 23 C. sakazakii isolates showed the same CFA profiles. The C. malonaticus isolate was discriminated from the C. sakazakii isolates by the significant difference in the amounts of $C_{12:0}$, $C_{14:0}$, and $C_{17:0\;cyclo}$ acids. These results showed that C. sakazakii and C. malonaticus were the common Cronobacter species distributed in PIF in China and that the isolates of the two species exhibited different CFA profiles. These findings are of value for epidemiological investigations and provide an alternative method for confirming various Cronobacter spp.
Kou Xiaoxia,Wu Qingping,Zhang Jumei,Fan Hongying The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.4
The purpose of this study was to determine the efficacy of a nucleic acid sequence-based amplification (NASBA) method of detecting noroviruses in artificially and naturally contaminated shellfish. We used 58 fecal samples that tested positive for noroviruses with electron microscopy (EM) to develop an NASBA assay for these viruses. Oligonucleotide primers targeting the polymerase coding region were used to amplify the viral RNA in an isothermal process that resulted in the accumulation of RNA amplicons. These amplicons were detected by hybridization with digoxigenin-labeled oligonucleotide probes that were highly specific for genogroup I (GI) and genogroup II (GII) of noroviruses. The expected band of 327bp appeared in denaturing agarose gel without any nonspecific band. The specific signal for each amplicon was obtained through Northern blotting in many repeats. All fecal samples of which 46(79.3%) belonged to GII and 12(20.6%) belonged to GI were positive for noroviruses by EM and by NASBA. Target RNA concentrations as low as 5pg/ml were detected in fecal specimens using NASBA. When the assay was applied to artificially contaminated shellfish, the sensitivity to nucleic acid was 100pg/1.5g shellfish tissue. The potential use of this assay was also confirmed in naturally contaminated shellfish collected from different ponds in Guangzhou city of China, of which 24 (18.76%) out of 128 samples were positive for noroviruses; of these, 19 (79.6%) belonged to GII and 5 (20.4%) belonged to GI. The NASBA assay provided a more rapid and efficient way of detecting noroviruses in fecal samples and demonstrated its potential for detecting noroviruses in food and environmental samples with high specificity and sensitivity.
Liu Shengrong,Wu Qingping,Zhang Jumei,Mo Shuping 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6
ε-Poly-L-lysine (ε-PL), produced by Streptomyces or Kitasatospora strains, is a homo-poly-amino acid of Llysine,which is used as a safe food preservative. In this study, the effects of L-lysine and its isomer, D-lysine, on ε-PL biosynthesis and their metabolites by the ε-PLproducing strain Streptomyces ahygroscopicus GIM8 were determined. The results indicated that L-lysine added into the fermentation medium in the production phase mainly served as a precursor for ε-PL biosynthesis during the flask culture phase, leading to greater ε-PL production. At an optimum level of 3 mM L-lysine, a ε-PL yield of 1.16 g/L was attained, with a 41.4% increment relative to the control of 0.78 g/L. Regarding D-lysine, the production of ε-PL increased by increasing its concentrations up to 6 mM in the initial fermentation medium. Interestingly, ε-PL production (1.20 g/L) with the addition of 3 mM D-lysine into the initial fermentation medium in flasks was higher than that of the initial addition of 3 mM L-lysine (1.06 g/L). The mechanism by which D-lysine improves ε-PL biosynthesis involves its utilization that leads to greater biomass. After S. ahygroscopicus GIM8 was cultivated in the defined medium with L-lysine, several key metabolites, including 5-aminovalerate, pipecolate, and L-2-aminoadipate formed in the cells, whereas only L-2-aminoadipate was observed after D-lysine metabolism. This result indicates that Llysine and D-lysine undergo different metabolic pathways in the cells. Undoubtedly, the results of this study are expected to aid the understanding of ε-PL biosynthesis and serve as reference for the formulation of an alternative approach to improve ε-PL productivity using L-lysine as an additional substrate in the fermentation medium.