Morphological, Agronomic, and Yield Characterization of Cluster Bean (Cyamopsis tetragonoloba L.) Germplasm Accessions

Aravind Kumar Jukanti,Ramakrishna Bhatt,Ramavtar Sharma,Rajwant Kaur Kalia 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.2

Cluster bean is an important legume crop of the arid and semi-arid regions of the world. In the present study, 140 germplasm lines of cluster bean were evaluated for agronomic and yield traits. Wide variation was observed among agronomic and yield-related traits among the accessions, making selection possible for different characters. High heritability (> 85%) coupled with high genetic advance (> 30%) was observed for yield per plant (TY), pods per cluster (PDSCL), and clusters on main branch (CLMN). Pod length (PDLT) and total pods per plant (TDP) explained 30% and 72% of variability in seed number per pod (SDN) and TY, respectively. Principal component analysis revealed significant variation among the characters with the first four principal components explaining about 70.8% of the total variation. Projecting the germplasm accession onto the first two principal components revealed two groups: (i) accessions showing high PDSCL, TPD, and TY and (ii) accessions having high SDN. Among the 140 germplasm lines evaluated, IC-421815 (TY - 31.5 g plant-1; TPD - 139.8; and PDSCL - 6.6) and IC-370563 (31.2 g plant-1, 134.0 and 7.4) were the best performing lines compared to the checks. The information presented in this manuscript would be used for cluster bean breeding.

A Gelucire 44/14 and labrasol based solid self emulsifying drug delivery system: formulation and evaluation

Venkata Raman Kallakunta,Basanth Babu Eedara,Raju Jukanti,Ram Kishan Ajmeera,Suresh Bandari 한국약제학회 2013 Journal of Pharmaceutical Investigation Vol.43 No.3

A solid self emulsifying formulation (S-SEF)has been developed with an intention to improve the dissolution characteristics of poorly water soluble lercanidipine hydrochloride (LH). Suitable components for the formulation of liquid self emulsifying drug delivery systems (SEDDS) were selected after screening various vehicles via solubility studies. Formulations were designed with Gelucire 44/14 as oil, labrasol as surfactant and transcutol-P as co surfactant. The prepared formulations were evaluated for self emulsifying efficiency and ternary phase diagram was used to designate optimum systems in the emulsifying domain. These systems were further investigated for robustness towards different pH conditions,globule size, thermodynamic stability, surface morphology,cloud point and in vitro drug release. The optimized LH loaded formulation possessed a mean globule size of 142.5 ± 5.37 nm and cloud point of 72 ±2.66 C. The liquid SEDDS was transformed into free flowing S-SEF by adsorbing on to an inert carrier, Neusilin US2. The results revealed no difference in globule size and emulsification characteristics between liquid SEDDS and S-SEF. The solid state characterization studies indicated loss of crystallinity for the drug. Significant improvement in dissolution characteristics of LH for prepared S-SEF was observed compared with pure drug.

Enhanced solubility and permeability of exemestane solid dispersion powders for improved oral delivery

Basanth Babu Eedara,Mradul Kankane,Raju Jukanti,Vijay Kumar Nagabandi,Suresh Bandari 한국약제학회 2013 Journal of Pharmaceutical Investigation Vol.43 No.3

The aim of the present investigation was to enhance the solubility of exemestane (EXM), by solid dispersion (SD) technique using PEG 6000 as a carrier. Phase solubility studies were conducted with PEG 6000and PEG 20000 to evaluate the effect of carriers on aqueous solubility of EXM. The aqueous solubility of EXM was favoured with PEG 6000 compared to PEG 20000. SDs of EXM using polyethylene glycol 6000 (PEG 6000) as carrier were prepared in different drug to carrier ratios. Solid-state characterization indicated decrease in crystallinity of the drug. The in vitro dissolution rate of EXM was enhanced from both SDs and tablet formulations prepared using SD compared to pure EXM. The in situ permeability studies investigated using single-pass intestinal perfusion technique in rats revealed increase in effective intestinal permeability (Peff, cm/s) by 4.45 folds with SDs. Thus, EXM-PEG 6000 SDs showed improved solubility and permeability.

Solubility Enhancement and Physicochemical Characterization of Carvedilol Solid Dispersion with Gelucire 50/13

Raja Hemanth Kumar Potluri,Suresh Bandari,Raju Jukanti,Prabhakar Reddy Veerareddy 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.1

The objective of the study was enhancement of dissolution of poorly soluble carvedilol by solid dispersions (SDs) with Gelucire 50/13 using solvent evaporation method. The solubility of carvedilol showed linear increase with increasing concentrations of Gelucire indicating AL type solubility diagrams. SDs characterized for physicochemical characteristics using differential scanning calorimetry and X-ray diffractometry revealed transformation of crystalline form of drug to amorphous form which was confirmed by scanning electron micrographs. Further fourier transform infrared spectroscopy results suggested there is no drug carrier interaction. From the dissolution parameters such as mean dissolution time, dissolution efficiency and drug release rate, improved dissolution characteristics for SDs were observed compared with physical mixture and pure drug. Thus SDs of carvedilol in Gelucire 50/13 showed enhanced solubility and dissolution rate compared to pure drug.

Proliposomes of lisinopril dihydrate for transdermal delivery: Formulation aspects and evaluation

Suresh Bandari,Swetha Gangishetty,Basanth Babu Eedara,Raju Jukanti,Prabhakar Reddy Veerareddy 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.8

We formulated and evaluated proliposomal gel of relatively low bioavailable drug lisinopril dihydrate (LDH)for transdermal delivery. Several proliposomal gel formulations of lisinopril dihydrate were prepared by modified coacervation phase separation method and examined for formation of liposomes by optical microscope and characterized by transmission electron microscopy. The formulations were evaluated for size, zeta potential, entrapment efficiency,rheological behavior, ex vivo drug permeation, skin irritation and stability. Differential scanning calorimetry (DSC)and Fourier transform infrared (FTIR) studies were performed to understand the phase transition behavior and mechanism for skin permeation, respectively. The microscopic examination revealed the formation of liposomes from proliposomal gel, and the size of the vesicles was found to be in the range of 385 nm to 635 nm. Entrapment efficiency was high for the formulation containing greater amounts of phosphatidylcholine. The DSC studies indicated the amorphous form of LDH in proliposomal gel formulation. Ex vivo permeation studies revealed sustained permeation of drug from proliposomal gels studied. The stability studies reveal that the proliposomal formulations are more stable when stored at refrigeration temperature (4 oC). In conclusion, proliposomal gels offer potential and prove to be efficient carriers for improved and sustained transdermal delivery of lisinopril dihydrate.

Physicochemical characterization and dissolution enhancement of loratadine by solid dispersion technique

Suresh Bandari,Subash Jadav,Basanth Babu Eedara,Raju Jukanti,Prabhakar Reddy Veerareddy 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.1

The purpose of this investigation was to enhance the dissolution rate of loratadine using polyethylene glycol 6000 (PEG) solid dispersions (SDs). The solubility behavior of loratadine in the presence of polyethylene glycol 4000and polyethylene glycol 6000 in water showed linear increase with increasing concentrations of PEG, indicating AL type solubility diagrams. SDs of loratadine with PEG 6000 were prepared at 1 : 1, 1 : 3, 1 : 5, 1 : 7 and 1 : 9 ratios by the solvent evaporation method. Solid dispersions were characterized for drug content, dissolution behavior and for physicochemical characteristics. The dissolution rate of loratadine was enhanced rapidly with increasing concentrations of PEG 6000 in SDs. Fourier transform infrared (FTIR) studies showed the stability of loratadine and the absence of a well-defined loratadine - PEG 6000 interaction. Differential scanning calorimetry (DSC) and X-ray powder diffractometry (XRD) studies revealed the amorphous state of loratadine in SDs of loratadine with PEG 6000 which was further confirmed from scanning electron microscopy (SEM) studies. The flow properties of the blend, physical characteristics and disintegration time of the tablets formulated indicated that PEG 6000 SD can be used to formulate fast release loratadine tablets.

Preparation and characterization of docetaxel self-nanoemulsifying powders (SNEPs): A strategy for improved oral delivery

Suresh Bandari,Sharath Sunkavalli,Basanth Babu Eedara,Karthik Yadav Janga,Ashok Velpula,Raju Jukanti 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.3

Liquid self-nanoemulsifying drug delivery systems (L-SNEDDS) of docetaxel were prepared using varying ratios of Capmul PG 8 NF (oil), Cremophor EL (surfactant) and Transcutol-P (co-surfactant). The optimized L-SNEDDS (L11) was transformed into self-nanoemulsifying powder (SNEP) by physical adsorption on to Neusilin US2 and evaluated for dissolution behavior, in vitro cytotoxicity and in vivo oral bioavailability. Optimized L-SNEDDS (L11) composed of 50% of oil, 41.7% of surfactant and 8.3% co-surfactant produced stable emulsion with smaller globules (43±3 nm). In vitro dissolution studies showed the rapid drug release within 5min (95.42±1%) from SNEPN. In vitro cytotoxicity assessed by the MTT assay using MCF-7 human breast cancer cell lines revealed that L-SNEDDS significantly reduced the IC50 value and was 2.3 times lower than the pure docetaxel. Further, the oral bioavailability studies in male Wistar rats showed higher Cmax values following treatment with SNEPN (0.98±0.13 μg/mL) and L-SNEDDS (1.09± 0.06 μg/mL) compared to pure docetaxel (0.37±0.04 μg/mL).