Deciphering the role of a membrane-targeting domain in assisting endosomal and autophagic membrane localization of a RavZ protein catalytic domain

( Jui-hee Park ),( Seung-hwan Lee ),( Sang-won Park ),( Yong-woo Jun ),( Kunhyung Kim ),( Pureum Jeon ),( Myungjin Kim ),( Jin-a Lee ),( Deok-jin Jang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2021 BMB Reports Vol.54 No.2

The bacterial effector protein RavZ from a pathogen can impair autophagy in the host by delipidating the mammalian autophagy- related gene 8 (mATG8)-phosphatidylethanolamine (PE) on autophagic membranes. In RavZ, the membrane-targeting (MT) domain is an essential function. However, the molecular mechanism of this domain in regulating the intracellular localization of RavZ in cells is unclear. In this study, we found that the fusion of the green fluorescent protein (GFP) to the MT domain of RavZ (GFP-MT) resulted in localization primarily to the cytosol and nucleus, whereas the GFP-fused duplicated-MT domain (GFP-2xMT) localized to Rab5- or Rab7-positive endosomes. Similarly, GFP fusion to the catalytic domain (CA) of RavZ (GFP-CA) resulted in localization primarily to the cytosol and nucleus, even in autophagy-induced cells. However, by adding the MT domain to GFP-CA (GFP-CA-MT), the cooperation of MT and CA led to localization on the Rab5-positive endosomal membranes in a wortmannin-sensitive manner under nutrient-rich conditions, and to autophagic membranes in autophagy- induced cells. In autophagic membranes, GFP-CA-MT delipidated overexpressed or endogenous mATG8-PE. Furthermore, GFP-CA_Δα3-MT, an α3 helix deletion within the CA domain, failed to localize to the endosomal or autophagic membranes and could not delipidate overexpressed mATG8-PE. Thus, the CA or MT domain alone is insufficient for stable membrane localization in cells, but the cooperation of MT and CA leads to localization to the endosomal and autophagic membranes. In autophagic membranes, the CA domain can delipidate mATG8-PE without requiring substrate recognition mediated by LC3-interacting region (LIR) motifs. [BMB Reports 2021; 54(2): 118-123]

DICOM-Based Multi-Center Electronic Medical Records Management System

Jui-Hung Kao,Chien-Yeh Hsu,Yu-Ping Sung,Wei-Pan Liao 보안공학연구지원센터 2010 International Journal of Bio-Science and Bio-Techn Vol.2 No.2

O, Shakespeare and Peking Opera Mixed, Reason in Madness

Jui-sung Chen 한국셰익스피어학회 2010 셰익스피어 비평 Vol.46 No.2

By fusing four skills and five methods of Chinese Peking Opera with William Shakespeare’s plays, Wu Hsing-kuo has been staging revolutionary performances in Taiwan in the past two decades. When Wu produced The Kingdom of Desire, adapted from Shakespeare’s Macbeth, in 1986, he was harshly challenged by his critics in three aspects: 1) the tradition of Peking opera and that of Shakespeare’s plays, 2) problem of translation, and 3) the advantages and disadvantages of integrating theatricality of the West with Peking Opera. In spite of all kinds of harsh criticism on his performances, Wu proclaimed himself as a master of the so-called New Peking Opera of Taiwan when staging King Lear in 2000. By analyzing how and why Wu wanted to parallel his own craze about theatre and Shakespeare’s Lear’s madness in the socio-historical context of Peking Opera, I propose to re-evaluate Wu’s artistic contribution to postmodern theatre in Taiwan. I would argue that Wu is not merely challenging the audience’s traditional attitudes toward Peking opera but also Taiwanese readings of Shakespeare, in a view to bringing new life to Taiwan’s contemporary theatre.

Robust Tracking Design Based on Adaptive Fuzzy Control of Uncertain Nonlinear MIMO Systems with Time Delayed States

Tzu-Sung Wu,Mansour Karkoub,Chien-Ting Chen,Wen-Shyong Yu,Ming-Guo Her,Jui-Yiao Su 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.6

It is proposed here to use a robust tracking design based on adaptive fuzzy control technique to control a class of multi-input-multi-output (MIMO) nonlinear systems with time delayed uncertainty in which each uncertainty is assumed to be bounded by an unknown gain. This technique will overcome modeling inaccuracies, such as drag and friction losses, effect of time delayed uncertainty, as well as parameter uncertainties. The proposed control law is based on indirect adaptive fuzzy control. A fuzzy model is used to approximate the dynamics of the nonlinear MIMO system; then, two on-line estimation schemes are developed to overcome the nonlinearities and identify the gains of the delayed state uncertainties, simultaneously. The advantage of employing an adaptive fuzzy system is the use of linear analytical results instead of estimating nonlinear system functions with an online update law. The adaptive fuzzy scheme uses a Variable Structure (VS) scheme to resolve the system uncertainties, time delayed uncertainty and the external disturbances such that H∞ tracking performance is achieved. The control laws are derived based on a Lyapunov criterion and the Riccati-inequality such that all states of the system are uniformly ultimately bounded (UUB). Therefore, the effect can be reduced to any prescribed level to achieve H∞ tracking performance. A two-connected inverted pendulums system on carts and a two-degree-of-freedom mass-spring-damper system are used to validate the performance of the proposed fuzzy technique for the control of MIMO nonlinear systems.

공공 연구개발(R&D)의 혁신을 위한 한국형 난제도전 R&D 모델에 관한 탐색적 연구

박주이(Jui Park),성창모(Changmo Sung) 한국혁신학회 2022 한국혁신학회지 Vol.17 No.4

The Major Changes of Gilbert’s Syndrome and UGT1A1 Gene Abnormalities in Mongolians Are Western Type

( Nyam Biziya ),( Nyamaa Bayarmaa ),( Jui-ting Hu ),( May-jen Huang ),( Ching-shan Huang ),( Sien-sing Yang ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Hereditary abnormalities of uridinediphosphoglucuronate-glucuronosyltransferase 1A1 (UGT1A1) gene is the major cause of unconjugated hyper-bilirubinemia. The abnormalities of UGT1A1 gene in Mongolian population remain uninvestigated. Eight in 99 consecutive Mongolian adults developed indirect hyperbilirubinemia. We therefore studied Mongolian patients for GS and UGT1A1 abnormalities. Methods: Between 2007 and 2014, ninety-nine consecutive Mongolian adult patients of chronic liver disease from the Department of Gastroenterology, Mongolian National University of Medical Sciences were studied. Eight (8.1%) of them developed indirect hyperbilirubinemia. All patients were tests for blood chemistries, hemoglobin, international normalized ratio (INR), mean corpuscular volume (MCV), glucose-6-phosphate dehydrogenase (G6PD) levels as well as UGT1A1 genetic abnormalities. We genotyped the UGT1A1 gene for the A(TA)6TAA (6) or A(TA)7TAA (7) promoter variant, and the coding region for nucleotide mutations (nt)-211 G to A, nt-686 C to A, nt-1091 C to T and nt-1456 T to G. Results: Among the eight patients that developed indirect hyperbilirubinemia, six were male and two were female. All patients had hemoglobin, INR, MCV and G6PD levels within normal limit and we excluded possibility of anemia, decompensated liver function, thalassemia and G6PD deficiency. Our data confirms two variants of the UGT1A1 gene among the Mongolian patients. Two case were homozygous for nt-211G>A mutation, two case heterozygous for 6/7 promoter variants and nt-211G>A mutation, whereas four case were typical GS with homozygous 7/7 promoter genotype with no mutation in the coding region None of our Mongolian patients had mutations at nt-686, nt-1091 or nt-1456. Conclusions: Our pilot results show that GS and UGT1A1 abnormalities are common in Mongolians. Prevalence of the UGT1A1 promoter abnormalities in Mongolians are similar to the Western population; whereas the high prevalence of nt-211G>A variant is similar to the Asians. Further studies with much larger number of patients are necessary to confirm the genetic status of GS and UGT1A1 variants in Mongolians.

Percutaneous Transvenous Inoue-Balloon Mitral Commissurotomy : an Update

Lau, Kean-Wah,Hung, Jui-Sung Yonsei cardiovascular center 2001 Korean journal of cardiovascular diseases Vol.2 No.2

The development of Taiwan Fracture Liaison Service network

Lo-Yu Chang,Keh-Sung Tsai,Jen-Kuei Peng,Chung-Hwan Chen,Gau-Tyan Lin,Chin-Hsueh Lin,Shih-Te Tu,I-Chieh Mao,Yih-Lan Gau,Hsusan-Chih Liu,Chi-Chien Niu,Min-Hong Hsieh,Jui-Teng Chien,Wei-Chieh Hung,Rong-S 대한골다공증학회 2018 Osteoporosis and Sarcopenia Vol.4 No.2

Osteoporosis and its associated fragility fractures are becoming a severe burden in the healthcare system globally. In the Asian-Pacific (AP) region, the rapidly increasing in aging population is the main reason accounting for the burden. Moreover, the paucity of quality care for osteoporosis continues to be an ongoing challenge. The Fracture Liaison Service (FLS) is a program promoted by International Osteoporosis Foundation (IOF) with a goal to improve quality of postfracture care and prevention of secondary fractures. In this review article, we would like to introduce the Taiwan FLS network. The first 2 programs were initiated in 2014 at the National Taiwan University Hospital and its affiliated Bei-Hu branch. Since then, the Taiwan FLS program has continued to grow exponentially. Through FLS workshops promoted by the Taiwanese Osteoporosis Association (TOA), program mentors have been able to share their valuable knowledge and clinical experience in order to promote establishments of additional programs. With 22 FLS sites including 11 successfully accredited on the best practice map, Taiwan remains as one of the highest FLS coverage countries in the AP region, and was also granted the IOF Best Secondary Fracture Prevention Promotion award in 2017. Despite challenges faced by the TOA, we strive to promote more FLS sites in Taiwan with a main goal of ameliorating further health burden in managing osteoporotic patients.

Visualizing anisotropic propagation of stripe domain walls in staircaselike transitions ofIrTe2

Mauerer, Tobias,Vogt, Matthias,Hsu, Pin-Jui,Pascut, Gheorghe Lucian,Haule, Kristjan,Kiryukhin, Valery,Yang, Junjie,Cheong, Sang-Wook,Wu, Weida,Bode, Matthias American Physical Society 2016 Physical Review B Vol.94 No.1

<P>We present a scanning tunneling microscopy (STM) study of the domain evolution across two first-order phase transitions of stripe modulations in IrTe2 that occur at T-C approximate to 275 K and T-S approximate to 180 K, respectively. Phase coexistence of the hexagonal (1 x 1) structure and the (5 x 1) stripe modulation is observed at TC, while various (p x 1) modulations (p = 3n + 2 with 2 <= n is an element of N) are observed below T-S. Using STM atomic resolution, we observe anisotropic propagation of domain boundaries along different directions, indicating significantly different kinetic energy barriers. These results are consistently explained by a theoretical analysis of the energy barrier for domain wall propagation as obtained by density functional theory. Individual switching processes observed by STM indicate that the wide temperature range of the transition from the (5 x 1) stripes to the (6 x 1)-ordered ground state is probably caused by the numerically limited subset of switching processes that are allowed between a given initial and the final state. The observations on IrTe2 are discussed in terms of a 'harmless staircase' with a finite number of first-order transitions between commensurate phases and within a 'dynamical freezing' scenario.</P>

Final report on international comparison APMP.QM-S4: Carbon dioxide in nitrogen at 2000 µmol/mol

Rhoderick, George,Kim, Yong Doo,Kim, Kwang Seob,Lee, Sangil,Oh, Sang Hyub,Lin, Tsai-Yin,Cheng, Jui-Hsiang,Huang, Chiung-Kun Springer-Verlag 2014 METROLOGIA -BERLIN- Vol.51 No.-

<P>This report presents the results of APMP.QM-S4, a supplementary comparison. This comparison is designed to test the capabilities of the NMIs to measure and certify carbon dioxide at a nominal amount fraction of 2000 µmol/mol in nitrogen. The previous key comparisons regarding carbon dioxide, CCQM-K1.b and CCQM-K52, had been done at a lower range than that in this comparison. This comparison gives an opportunity to the NMIs to submit supporting evidence for CMC at a higher range.</P><P>Main text.To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.</P><P>The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).</P>