Enhanced Stability of Hydroxyapatite-coated Liposomes for Ultrasound-triggered Drug Release

Jueng Ha,조성근,박은석,한건,한희동,신병철 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.1

Thermosensitive liposomes (TSLs) have been developed for enhancing drug release in the tumor microenvironment by external stimulations, such as hyperthermia, for cancer chemotherapy. However, the TSL-induced side effect of the drug on normal cells after intravenous (i.v.) injection is usually found in circulation. Therefore, we developed sterically stabilized TSLs by coating hydroxyapatite (HA) onto the liposomal surface to increase its stability in serum. HA coating on the liposomal surface was achieved via the ionic interaction of calcium acetate with phosphoric acid. The amount of HA coated on the liposomal surface was measured by inductively coupled plasma (ICP) spectroscopy. In addition, temperature-sensitive drug release was measured by fluorescence spectrophotometry. The HA-coated TSL (HA-TSL) showed lower drug release at body temperature due to the HA coating on the liposomal surface compared to TSL. Moreover, compared with TSL, HA-TSL showed increased stability in serum, thereby indicating a long circulation time in the body without protein adsorption. Taken together, we demonstrated the use of HA coating on the surface of TSL for enhancing the stability of TSL, and the results indicated the applicability of the method for prolonging the circulation of drugs with carriers within the human body. As a novel and highly effective delivery platform with long circulation in blood streams, HA-TSL shows great potential for use in a broad range of applications for the treatment of various human diseases.

천식 소아에서 혈중 포스포리파제 A2: leptin과 운동유발 기관지과민성과의 상관성

유정섭 ( Jueng Sup You ),최원복 ( Won Bok Choi ),이윤영 ( Yoon Young Yi ),정수인 ( Soo In Jeong ),송준섭 ( Joon Sup Song ),양승 ( Seong Yang ),황일태 ( Il Tae Hwang ),이하백 ( Ha Baik Lee ),백혜성 ( Hey Sung Baek ) 대한천식알레르기학회 2015 Allergy Asthma & Respiratory Disease Vol.3 No.2

Purpose: Dysregulated cysteinyl leukotriene (CysLT) synthesis is prominent in exercise-induced bronchoconstriction (EIB). Secreted phospholipase A2 (sPLA2) plays a key regulatory role in the biosynthesis of CysLTs. We previously found that serum leptin levels cor￢relate with (EIB) in children with asthma. The aim of this study was to address the relationship between plasma sPLA2/leptin levels and EIB. Methods: Sixty-seven prepubertal children between the ages of 6 and 10 years were included in the study. They were asthmatics with EIB (n=25), asthmatics without EIB (n=21), and healthy subjects (n=21). We measured the plasma sPLA2 and leptin levels. We also performed pulmonary function tests at baseline, after bronchodilator inhalation, and after exercise. Results: The sPLA2 and leptin levels were significantly higher in asthmatics with EIB than in those without and control subjects. In addition, sPLA2 levels were significantly correlated with body mass index (Speraman correlation coefficient r=0.343, P=0.023) and leptin levels (partial correlation coefficient r=318, P=0.033). The maximum decrease in % forced expiratory volume in 1 second af￢ter exercise was significantly correlated with both PLA2 levels (r=0.301, P=0.041) and leptin levels (r=0.346, P=0.018). Conclusion: The sPLA2 and leptin levels were significantly higher in asthmatics with EIB than in asthmatics without EIB and control subjects. In addition, sPLA2 levels were significantly correlated with leptin levels and EIB in asthmatic children.(Allergy Asthma Respir Dis 2015;3:99-104)

Effects of Atomoxetine on Hyper-Locomotive Activity of the Prenatally Valproate-Exposed Rat Offspring

( Chang Soon Choi ),( Min Ha Hong ),( Ki Chan Kim ),( Ji Woon Kim ),( Sung Min Yang ),( Hana Seung ),( Mee Jung Ko ),( Dong Hee Choi ),( Jueng Soo You ),( Chan Young Shin ),( Geon Ho Bahn ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.5

A substantial proportion of patients with autism spectrum disorder (ASD) display hyperactivity as a comorbid symptom. Exposure to valproic acid (VPA) during pregnancy produces ASD-like core behavioral phenotypes as well as hyperactivity in offspring both in human and experimental animals, which makes it a plausible model to study ASD-related neurobiological processes. In this study, we examined the effects of two of currently available attention defecit hyperactivity disorder (ADHD) medications, methylphenidate (MPH) and atomoxetine (ATX) targeting dopamine and norepinephrine transporters (DAT and NET), respectively, on hyperactive behavior of prenatally VPA-exposed rat offspring. In the prefrontal cortex of VPA exposed rat offspring, both mRNA and protein expression of DAT was increased as compared with control. VPA function as a histone deacetylase inhibitor (HDACi) and chromatin immunoprecipitation experiments demonstrated that the acetylation of histone bound to DAT gene promoter was increased in VPA-exposed rat offspring suggesting epigenetic mechanism of DAT regulation. Similarly, the expression of NET was increased, possibly via increased histone acetylation in prefrontal cortex of VPA-exposed rat offspring. When we treated the VPA-exposed rat offspring with ATX, a NET selective inhibitor, hyperactivity was reversed to control level. In contrast, MPH that inhibits both DAT and NET, did not produce inhibitory effects against hyperactivity. The results suggest that NET abnormalities may underlie the hyperactive phenotype in VPA animal model of ASD. Profiling the pharmacological responsiveness as well as investigating underlying mechanism in multiple models of ASD and ADHD may provide more insights into the neurobiological correlates regulating the behavioral abnormalities.

자작나무에 감작된 소아에서 과일 알레르기를 진단하기 위한 microarray법에 의한 성분 항원검사

최원복 ( Won Bok Choi ),유정섭 ( Jueng Sup You ),이윤영 ( Yoon Young Yi ),정수인 ( Soo In Jeong ),송준섭 ( Joon Sup Song ),양승 ( Seong Yang ),황일태 ( Il Tae Hwang ),이하백 ( Ha Baik Lee ),백혜성 ( Hey Sung Baek ) 대한천식알레르기학회 2015 Allergy Asthma & Respiratory Disease Vol.3 No.3

Purpose: Recently, component-resolved diagnosis (CRD) using microarray technology has been introduced to the field of clinical allergy. This study was aimed to investigate the clinical usefulness of microarray-based IgE detection for diagnosing clinical raw fruit allergy in birch pollen-sensitized children. Methods: Thirty-one children with allergic disease who had been sensitized to pollen were studied. A pollen-sensitized patient was defined as having an allergen-specific history with concomitant positive skin-prick tests (SPTs) to natural allergen extracts or positive allergen-specific IgE. All subjects underwent SPTs for pollen and fruit. In all subjects, specific IgE to pollen and fruit were measured by ImmunoCAP. Specific IgE antibodies to allergen components were determined by a customized allergen microarray (ISAC). Results: Thirteen of the 31 patients (41.9%) had a history of fruit hypersensitivity with positive SPTs. Measuring IgE to allergen components by ISAC, all the 13 patients with fruit hypersensitivity were positive to at least one of Mal d 1, Pru p 1, Pru p 3, Act d 8, and Act d 2 compared to 12 of the 13 patients (92.3%) who had at least 1 positive IgE to fruits (apple, peach, and kiwi) using ImmunoCAP. The sensitivity of ISAC microarray was 100.0% for the diagnosis of fruit hypersensitivity, but its specificity was 27.7% (5/18). The sensitivity of ImmunoCAP was 92.3%, and its specificity was 83.3%. Conclusion: The sensitivity of allergen components tested using microarray for the diagnosis of clinical fruit hypersensitivity in children with pollen allergy was high; however, its specificity was low.(Allergy Asthma Respir Dis 2015;3:200-205)

The Amniotic Fluid Proteome Differs Significantly between Donor and Recipient Fetuses in Pregnancies Complicated by Twin-to-Twin Transfusion Syndrome

김선민,Byoung-Kyu Cho,Byoung Jae Kim,Ha Yun Lee,Errol R. Norwitz,Min Jueng Kang,Seung Mi Lee,박찬욱,Jong Kwan Jun,Eugene C. Yi,박중신 대한의학회 2020 Journal of Korean medical science Vol.35 No.10

Background: Twin-to-twin transfusion syndrome (TTTS) is a serious complication of monochorionic twin pregnancies. It results from disproportionate blood supply to each fetus caused by abnormal vascular anastomosis within the placenta. Amniotic fluid (AF) is an indicator reflecting the various conditions of the fetus, and an imbalance in AF volume is essential for the antenatal diagnosis of TTTS by ultrasound. In this study, two different mass spectrometry quantitative approaches were performed to identify differentially expressed proteins (DEPs) within matched pairs of AF samples. Methods: We characterized the AF proteome in pooled AF samples collected from donor and recipient twin pairs (n = 5 each) with TTTS by a global proteomics profiling approach and then preformed the statistical analysis to determine the DEPs between the two groups. Next, we carried out a targeted proteomic approach (multiple reaction monitoring) with DEPs to achieve high-confident TTTS-associated AF proteins. Results: A total of 103 AF proteins that were significantly altered in their abundances between donor and recipient fetuses. The majority of upregulated proteins identified in the recipient twins (including carbonic anhydrase 1, fibrinogen alpha chain, aminopeptidase N, alpha-fetoprotein, fibrinogen gamma chain, and basement membrane-specific heparan sulfate proteoglycan core protein) have been associated with cardiac or dermatologic disease, which is often seen in recipient twins as a result of volume overload. In contrast, proteins significantly upregulated in AF collected from donor twins (including IgGFc-binding protein, apolipoprotein C-I, complement C1q subcomponent subunit B, apolipoprotein C-III, apolipoprotein A-II, decorin, alpha-2-macroglobulin, apolipoprotein A-I, and fibronectin) were those previously shown to be associated with inflammation, ischemic cardiovascular complications or renal disease. Conclusion: In this study, we identified proteomic biomarkers in AF collected from donor and recipient twins in pregnancies complicated by TTTS that appear to reflect underlying functional and pathophysiological challenges faced by each of the fetuses.

이질 시스템에서 통신 시간을 고려한 효율적인 복제 기반 태스크 스케줄링

윤완오(Wan-Oh Yoon),백정규(Jueng-Kuy Baek),신광식(Kwang-Sik Shin),정진하(Jin-Ha Cheong),최상방(Sang-Bang Choi) 한국통신학회 2008 韓國通信學會論文誌 Vol.33 No.3C

스케줄링의 목적은 입력 작업(DAG)에 대한 스케줄 결과 길이를 최소화 하는 것이다. 이런 스케줄링 문제는 잘 알려진 '정해진 시간 내에 해결하기 어려운 문제(NP-complete)'이며 최적의 스케줄링 결과 값을 얻기 위해서는 휴리스틱으로 해결해야 한다. 선후 관계의 제약을 갖는 노드들의 스케줄링을 효율적으로 수행하기 위해 부모 노드와 이질 프로세서에 대한 정보를 고려하는 TANH(the Task duplication based scheduling Algorithm for Network of Heterogeneous systems), GDL, BIL, TDS과 같은 많은 알고리즘이 제안되었다. 본 논문은 기존의 TANH 스케줄링에서 나타나는 여러 개의 부모 노드와 이질 프로세서에 대한 다양한 경우를 충분히 고려하지 못한 점을 보안하여 향상된 스케줄링을 수행할 수 있는 DTSC (Duplication based Task Scheduling with Communication Cost in Heterogeneous Systems)알고리즘을 제안하였다. 제안된 알고리즘의 성능은 기존 TANH, GDL 알고리즘과 비교하였으며, 스케줄링의 성능 향상을 보여 주었다. Optimal scheduling of parallel tasks with some precedence relationship, onto a parallel machine is known to be NP-complete. The complexity of the problem increases when task scheduling is to be done in a heterogeneous environment, where the processors in the network may not be identical and take different amounts of time to execute the same task. This paper introduces a Duplication based Task Scheduling with Communication Cost in Heterogeneous Systems (DTSC), which provides optimal results for applications represented by Directed Acyclic Graphs (DAGs), provided a simple set of conditions on task computation and network communication time could be satisfied. Results from an extensive simulation show significant performance improvement from the proposed techniques over the Task duplication-based scheduling Algorithm for Network of Heterogeneous systems(TANH) and General Dynamic Level(GDL) schedluling algorithm.

천식 소아에서 혈청 렙틴과 만니톨 기관지 과민성과의 관계

유정경 ( Jung Kyung Yoo ),신재영 ( Jae Young Shin ),유정섭 ( Jueng Sup You ),정수인 ( Soo In Jeong ),송준섭 ( Joon Sup Song ),양승 ( Seong Yang ),황일태 ( Il Tae Hwang ),이하백 ( Ha Baik Lee ),백혜성 ( Hey Sung Ba다 ) 대한천식알레르기학회 2014 Allergy Asthma & Respiratory Disease Vol.2 No.1

Purpose: Epidemiological data indicate that obesity is a risk factor in asthma, however effects related to obesity and adipokines on airway inflammation and bronchial hyper-responsiveness (BHR) have not yet been demonstrated in the human airway. The aim of this study was to investigate the relationship between serum adipokine levels and BHR to mannitol in asthmatic children. Methods: Serum adipokine levels were measured and pulmonary function tests were perfomed: baseline, postbronchodilator inhalation, methacholine inhalation, and mannitol inhalation. The response to mannitol was expressed as the dose causing a 15% decrease in forced expiratory volume in one second (FEV1) (PD15), and as the response-dose ratio (RDR) (% fall in FEV1/cumulative dose). Results: Sixty-nine prepubertal children between the ages of 6 and 10 years were participated in the study. They comprised asthmatic children (n=40) and healthy (n=29). Twenty-two subjects (55.5%) with asthma had a positive mannitol bronchial provocation test (BPT) result. The body mass index (BMI) was higher in those asthmatics with positive mannitol BPTs than in asthmatics with negative mannitol BPTs and in the control group (19.30 kg/m2 vs. 17.60 kg/m2 vs. 17.93 kg/m2, P=0.035, P=0.046). Serum leptin levels were also significantly higher in asthmatics with positive mannitol BPTs than in asthmatics with negative mannitol BPTs and in the control group (10.58 ng/mL vs. 5.49 ng/mL vs. 6.75 ng/mL, P=0.002, P=0.016). Leptin values were significantly associated with a PD15 (r=.0.498, P=0.022) and RDR to mannitol (r=0.346, P=0.033) in asthmatic children after adjustment for BMI. Conclusion: Serum leptin levels were significantly associated with BHR to mannitol in asthmatic children. (Allergy Asthma Respir Dis 2014;2:30-37)

필지단위 관개용수 공급에 따른 농업가뭄진단 평가

신지현 ( Shin Ji-hyeon ),남원호 ( Nam Won-ho ),김하영 ( Kim Ha-young ),문영식 ( Mun Young-sik ),방나경 ( Bang Na-kyoung ),이정철 ( Lee Jueng-Chol ),이광야 ( Lee Kwang-ya ) 한국농공학회 2021 한국농공학회논문집 Vol.63 No.4

Agricultural drought is a natural phenomenon that is not easy to observe and predict and is difficult to quantify. In South Korea, the amount of agricultural water used is large and the types of use are varied, so even if an agricultural drought occurs due to insufficient precipitation, the drought actually felt in the irrigated area is it can be temporally and spatially different. In order to interpret the general drought in the past, drought disasters were evaluated using single indicators such as drought damage area, precipitation shortage status, and drought index, and a comprehensive drought management system is needed through drought diagnosis survey. Therefore, we intend to conduct research on agricultural drought assessment and diagnosis using re-evaluation of agricultural facilities and irrigation water supply network due to changes in various conditions such as climate change, irrigation canal network, and evaluation of water supply capacity of agricultural facilities. In this study, agricultural drought diagnosis was conducted on two agricultural reservoirs located in Sangju, Gyeongsangbuk-do, with structural or non-structural evaluations to increase spatiotemporal water supply and efficiency in terms of water shortages. The results of the agricultural drought diagnosis evaluation can be used to identify irrigated areas and canal network vulnerable to drought and to prioritize drought response.