PCR을 이용한 Rat 기관지 세척액에서의 Pneumocystis carinii DNA의 검출 : 기초실험 A pilot study

김주옥,홍석철,한표성,이종진,김선영,이영하,신대환 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

Background: Pneumocystis carinii (PC) is a opportunistic pathogen causing serious pneumonia (Pneumocystis carinii pneumonia : PCP) in immunosuppressed patients including AIDS. Laboratory diagnosis of PCP is dependent on microscopic demonstration of the PC by using cytochemical staining or by immunocytochemical staining with monoclonal antibodies. However, these staining methods are not highly sensitive. The development of the polymerase chain reaction (PCR) has made it possible to detect very small numbers of pathogens in clinical specimens. To establish the usefulness of PCR for detection of PC DNA, We performed PCR as a pilot study. Methods: PCP was induced on Spraque-Dawley rats with prednisolone 5mg IM twice a week. Bronchial lavages, impression smears, and permanent sections were performed from the 3rd week to 7th week of cortisonized rats. PC DNA was extracted with bead beater / 10% CTAB method. Results: Impression smears showed PC cyst after the 4th week (100%, 28/28 rats), and permanent sections showed 27/28 rats. Control rats showed some PC cysts (2/7). PCR result was positive only one case among the 6th week rats (1/7), But positive 6 cases at the 7th week rats, (6/7). Among control rats. 2 cases were positive (2/7). ??This data is a pilot study for the PC DNA detection using a PCR. PCP were successfully cortisonized rats. If adequate bronchial washing technique will be used, PCR can be a sensitive for PC DNA detection.

肺癌患者에 있어서 治療前 後의 免疫機能 變異에 關한 硏究

김주옥,이복희 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.1

To analysis the changes of peripheral leukocyte count, lymphocyte count, percent of lymphocyte and T cell subsets(OKT₃, OKT₄, OKT_8, OKT₄,/OKT_8) in patients with bronchogenic carcinoma following treatment, a study was performed in 49 untreated and 23 treated (followed up) patients with bronchogenic carcinoma. The results were summarized as follows. 1. In the untreated group, peripheral lymphocyte count, percent of lymphocyte and OKT_8 were decreased compared to normal control. According to stage, OKT_8 was decreased in all stages of non-small cell cancer, OKT₄,/OKT_8 was increased in stage III significantly. According to histology, OKT_8 was decreased in squamous, adenocarcinoma and small cell carcinoma, and OKT₄,/OKT_8 was increased in adenocarcinoma significantly. 2. In the treated (followed up) group, OKT₄,/OKTB was decreased to normal range, which was more apparent in stage III. 3. According to mode of response, OKT_8 was decreased significantly compared to normal control in responder and nonresponder before treatment, and was persistently decreased in nonresponder after treatment. In responder, increased lymphocyte count and decreased OKT₃ were observed compared to nonresponder before treatment. In nonresponder, OKT₃ and OKT₄, were decreased significantly after treatment compared to before treatment. In conclusion, immunoabnormalities in patients with lung cancer might be improved following treatment, but further studies for quantitative and functional changes in lymphocytes following treatment (including radiation) are needed to analyse the role of immunologic changes for progression and recurrence of cancer and for the prognosis of the cancer patients.

기관지경 생검상 확진된 폐암환자에서 기관지세척 세포진과 다른 지표들과의 관계

김주옥,서지원 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

Bronchoscopic examination is an essential tool for diagnosis and treatment of the lung cancer. Various method with bronchoscopy are possible, such as cytologic examination (washing, brushing, bronchoalveolar lavage and transbronchial needle aspiration0, mucosal biopsy and transbronchial lung biopsy. There are many reports on the bronchial washing cytology, but there were few reports on the relations between bronchial washing cytology and other various parameters(age, sex, celltype, bronchoscopic location and shape of lesion). To evaluate the relationships between washing cytologic yield, age, sex, celltype, bronchoscopic location and shape of lesion in lung cancer, we have studied in 189 patients(males 170, females 19; ages, mean 60.7yrs) with histologically proven lung cancer who showed direct signs of lung cancer bronschoscopically. All cases were collected washing cytology specimens simultaneously. :There were no significant relationship between age and washing cytology positivity, celltype or bronchoscopic shape of lesion, between sex and washing cytology positivity, bronchoscopic location or shape of lesion, There was significant relationship between sex and celltype, that is, NSCLC in male vs SCLC in female. There were no significant relationship between cytology positivity and celltype, bronchoscopic location or shape of lesion. There was significant relationship between celltype and bronchoscopic location of lesion, that is, squamous and SCLS in both upper lobes va adenocarcinoma in RLL. There was no significant relationship between bronshoscopic location and shape of lesion, but significant between celltype and shape of lesion.(mass in squamous vs non-mass in SCLC). In conclusion, lung cancer has more prevalent in older males than females and both upper lobes, and squamous cell carcinoma has more mass-shaped and SCLS has more infiltrated lesion bronchoscopically. The cytologic positivity was not depended on the bronchoscopic shape of lesion.

결핵환자에 있어서 세포성 면역의 변화 (1)

김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

It is well established that cell-mediated immunity including T cell activation is the most important mechanism for the host defence against Mycobacterium tuberculosis. There are some indices for describing the mitogen-induced lymphocyte transformation such as stimulation index (SI). Recently curnmulative index (CI) was suggested to describe lymphocyte blastogenic function more accurately. To evaluate the abnormalities of cell-mediated immunity and usefulness of CI, we measured the T cell subsets and compared the indices (SI and CI) of lymphocyte transformation of peripheral mononuclear cells in patients with tuberculous patients (17 with pulmonary tuberculosis, 5 with tuberculous pleurisy). The postitive scores of skin test using CMI multitest kit were significantly decreased in patients group compared to normal controls. The percentage of total T lymphocytes in patients was not significantly different from normal controls, but that of helper T cells was significantly decreased and that of suppressor T cells was significantly increased, resulting significant decrease in T_4/T_8 ratio in patients group. The SI in patients was not significantly different from controls because of wide standard deviation, but CT was significantly decreased in patients group. As a result of this study, we could confirm the abnormal cell-mediated immunity in tuberculous patients and suggest that CI is a more useful parameter for describing the mitogen-induced lymphocyte transformation.

大都市 低所得層 幼兒의 營養에 關한 考察 : Based on the Data of Nutivition Survey through 1977-1986 1977-86年 10年間의 營養實態 調査를 中心으로

杜玉珠 서울大學校保健大學院 1994 國民保健硏究所硏究論叢 Vol.4 No.2

This study have reviewed the nutritional status of pre-school children of the low income area in Seoul, based on the survey data from 1977 to 1987. The nutritional status of children was assessed by anthropometric method and diet method. The results of the study were summarized as follow: 1. About 47 percentage of pre-school children of the low income area in Seoul corresponded to mild malnutritional stage assessed by anthropometric method in 1977. It decreased to 4% in 1983. 2. Assessed by diet method, their total daily calori intakes were in normal range compared to recommended dietary allowance. But their daily intake amounts of protein, calcium, iron and vitamins(except thiamin) represented defficient compared with recommended dietary allowance and those of children of other area in Seoul. These results implicate that their inferior physical development condition was caused by their poor nutritional status. Therefore, nutritional education programs should be planned and performed for their parents and the quality and extent of day care lunch such area should be improved.

객혈의 임상적 고찰 및 기관지 내시경의 역할

김주옥,이종진,서지원,김선영 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

118 patients with hemoptysis were reviewed retrospectively to identify etiologic factors, clinical features, and treatment and to evaluate the usefulness of fiberoptic bronchoscopy. 63 cases (53.4%) were blood-tinged sputum, 2 cases (1.7%) were more than 600ml a day in amount of hemoptysis. Pulmonary tuberculosis was 37 cases (31.36%), the most common cause of the known etiologic factors. Lung cancer was 6 cases (5.09%). 6 patients (5.09%) were treated with bronchial artery embolization, 1 patient (0.85%) treated with right lower lobectomy. 1 (4.3%) of 23 patients with hemoptysis and normal chest roentgenogram was found to have lung cancer. We suggest that in patients with hemoptysis and normal chest roentgenogram, routine fiberoptic bronchoscopy is not necessary to exclude malignancy.

환자복의 색상이 환자에게 미치는 심리적인 영향

함옥상,이혜주 啓明大學校 生活基術硏究所 1997 科學論集 Vol.23 No.-

<Abstract>This study was aimed to evaluate the psychological effect of patient's clothing on patients. The results were gathered through and assessed questionnaires for patients who were admitteed in university hospitals about the relationship between the degree of concern about patient's clothes and emotional factors of the color, and the emotional status of the patient at the time of study.The results were as follows;1. In the relationship between the color of patient's clothes and their satisfaction with design, the color and design were interested in older people in age and married patients, and the most satisfactory clothes was those with the orange color and the abstract pattern.2. Favorite patterns of design were favorite check, flowered, and abstract. White was preferreed by males and green by females.3. In the affinity of the patient's clothes, those who show an affinity for clothing also show an increased interest in their clothing when they are a patient. The color of their clothing affected their recovery.4. The degree of concern about patient's clothes was highger with abstract patterns and in the fiftieth decade rather than in the tenth.5. The patient's emotional status was affected by the color image of their clothing. Patients who were emotionally unstable had negative images, but those with increased satisfaction with color and design had a more positive image.6. The color of patient's clothing had a greater effect on recovery of the disease in the emotionally unstable patient.

공간구문론을 이용한 한옥 생활공간의 변화에 대한 해석 : 충청지역 중요민속자료 지정 한옥을 대상으로 Based on a Case study of Important Folklore Materials in Chungcheong Province

이주옥,한필원 대한건축학회 2003 대한건축학회논문집 Vol.19 No.8

The objective of this study is to analyze spatial changes of traditional Korean houses in Chuncheong province that are designated as National Cultural Properties. This study is based on the premise that the residents of these houses were forced to modify their dwelling spaces in order to bridge the gap between the traditional spaces and the current living pattern, though change was constrained by relevant regulations. In this study, it was found that Space Syntax effectively the relative spatial depth of the elements composing honok. Thus, Space Syntax was adopted as an analytic method of the study to objectively measure the spatial changes of the seven case houses. In this study, the present spatial organization of each case is compared with that of 20 years ago to find out how the organization and use of space has changed over the past two decades. The results are summarized as follows.: The meas RRA of all spatial elements has increased in all cases over the 20 years period. Especially, the increase ratio of the mean RRA is exceeded by that of the RRA of both Anbang and maru/daecheong, in most cases. On the other hand, the increase ratio of the RRA of kitchen is exceeded by that of the mean RRA in most cases. The RRA of kitchen was bigger than that of anbang in the past, which is reversed at the present. The above findings suggest that the anbang has come to be a more private space, whereas the maru/daecheong have come to be more closed. On the other hand, the kitchen has become more accessible to/from the interior spaces.

폐암환자에 있어서 T 세포 매개성 면역의 변화(Ⅰ)

김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

Cell-mediated immunity (CMI) was evaluated in 30 patients with lung cancer by in vivo and in vitro methods. These included skin test with CMI multitest kit, determination of peripheral T lymphocytes, and lymphocyte response to phytohemagglutinin (PHA) and concanavalin A (Con A). The positive scores of skin test were significantly lower in lung cancer group than controls (p<0.05), and there were no significant differences in T cell subsets. The response to PHA and Con A were greatly depressed in lung cancer and it was more apparent in advanced stage. But there was no difference in stimulation index (SI) between cancer patients and normal controls. The cumulative index (Cl) was more significant difference in cancer patients and advanced stage. These results suggest that depressed CMI in patients with lung cancer might be caused by lymphocytic dysfunction and cumulative index is more accurate to describe lymphocyte blastogenic function than stimulation index.

고상추출카트리지를 이용한 잔류농약 다중분석과정에서 정제용매에 따른 회수율 비교

두옥주,정보경,김경식,정의근,김진곤,김명희 한국환경농학회 2002 한국환경농학회지 Vol.21 No.2

채소류 중 비교적 matrix효과가 적고, 수분 함량이 적절하여 회수율이 양호한 오이를 대상으로 하여 총 16종의 농약 성분을 A, B 두 group으로 나누어, 농약의 동시 다성분 분석의 전처리 과정 중 정제에 사용하는 용출용매로서 5%~20%의 acetone/hexane과 5%~20%의 ethyl acetate/hexane을 사용하여 각각의 회수율을 분석하여 얻은 결과는 다음과 같다. Acetone/hexane을 사용한 경우 5% acetone에서 총 16종의 농약 성분 중 ethalfluralin, trifluralin, chlorothalonil 등 11종의 농약은 높은 회수율을 보였으나, alachlor와 iprodione, endosulfan sulfate, bifenthrin, fenarimol은 매우 낮은 회수율을 나타내었고, 10% 이상의 acetone/hexane에서는 bifenthrin과 fenarimol 제외한 14종의 농약에서 높은 회수율을 얻을수 있었다. 그러나 bifenthrin은 20%의 acetone/hexane을 사용한 경우는 용출되지 않았고, fenarimol은 10% 이하의 acetone/hexane을 사용하였을 때는 낮은 회수율을 나타냈었다. Ethyl acetone/hexane을 사용하여 총 16 종의 농약 성분의 회수율 분석을 한 결과, 5% ethyl acetone에서 ethalfluralin, α-endosulfan, β-endosulfan (A group), vinclozolin, tetradifon, cyprethrin, fenvalerate (B group)에서만 70% 이상의 회수율을 얻었다. 10%의ethyl acetone/hexane을 사용하여 분석한 경우는 trifuralin, chlorothalonil, α-endosulfan, β-endosulfan, endosulfan sulfate(A group), vinclozolin, procymidone, tetradifon, cypermethrin, 그리고 fenvalerate(B group) 에서는 70% 이상의 회수율을 얻었고, 20%의 cthyl acetone/hexane 을 사용하여 분석한 경우는 5%나 10%의 ethyl acetone/hexan 을 사용하여 분석했을 때보다 좋은 결과를 나타내어 전체 16종의 농약 중 14종에서 75% 이상의 회수율을 얻었으나, alachlor와 bifenthrin은 매우 낮은 회수율을 보여서 ethyl acetate 와 hexane의 혼합용매가 이들 두 농약성분의 분석에는 적합하지 않은 용출 용매임을 알 수 있었다. A study was carried out to optimize the cleanup step using a solid-phase extraction (SPE) cartridge, SupelClean^(TM) Florisil, for multiresidue analysis of 16 pesticides in cucumber matrix. Eluting efficiencies of two solvent systems including acetone/n-hexane and ethyl acetate/n-hexane mixtures were critically evaluated by recoveries of target anaytes from the SPE Florisil column. Based on the recovery as a measure of eluting efficiency, an acetone/n-hexane (20/80,v/v) mixture provided more than 80% recovery for 15 pesticides except bifenthrin. In case of ethyl acetate/n-hexane, 14 pesticides showed recoveries higher than 75%, while those for alachior and bifenthrin were less than 30%.