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      • 상호상관 함수를 이용한 지중선로 고장점 추정

        朴星完,林泳範,鄭州桓,成洛煥,金喆煥,申明徹 成均館大學校 科學技術硏究所 1994 論文集 Vol.45 No.2

        This paper is intended as an investigation of fault location for underground line. Over the past few decades a considerable number of studies have been made on protection of underground line. However, these approaches require the interruption of electric power supply from source for fault location estimation. Therefore, we proposed fault location algorithm for underground line protection without interruption of electric power supply. Cross correlation function is used in this algorithm. Also, EMTP data is used to demonstrate the capability of the proposed fault location algorithm.

      • SCOPUSKCI등재
      • KCI등재후보
      • Serratia marcescens에서 cAMP receptor protein(CRP)유전자의 클로닝 및 해석

        유주순,문종환,정수열,김혜선,최용락 東亞大學校附設遺傳工學硏究所 1999 遺傳工學硏究 Vol.- No.6

        전사조절인자로서 잘 알려져 있는 cAMP receptor protein(CRP)은 cAMP와 DNA에 결합하는 특별한 활성을 가지고 있으며, cAMP-CRP complex를 형성하여 수많은 유전자의 발현조절에 관여한다. 이러한 측면에서 cAMP-CRP의 조절은 어떤 면에서 총체적 조절체계라고까지 한다. 본 연구는 Serratia 균주에서 crp유전자의 분자적 특성 및 cAMP에 의한 발현조절을 받는 분자기구를 해석하고자 유전자를 클로닝하고 발현을 확인하였다. MacConkey배지에서 maltose를 탄소원으로 충분히 이용하지 못하는 대장균 TP2139(△crp,△lac)를 숙주로 이용하고, 염색체DNA를 library로 작성하여 얻은 형질전환체 약 일만개의 콜로니에서 red colony를 나타내는 5종류 의 양성 클론을 얻었다. 이들 클론을 Southern방법으로 확인한 결과 3kb의 단편을 가진 pCKB12클론이 crp유전자를 coding하고 있음을 확인하였다. glpD-lacZ 융합 plasmid인 pLDC6 의 BamHI부위에 pCKB12의 3kb 단편을 삽입시킨 재조합 plasmid pLDC 6-Scrp를 작성하여, 클로닝된 Serratia 의 crp 유전자가 대장균에서 유전자 전사조절에 미치는 영향을 확인한 결과 cAMP-CRP 복합체 형성에 의한 전사조절 기능이 확인되어졌다. One of the better-characterized transcription factor of E. coli is the cAMP receptor protein(CRP) and the CRP binds cAMP and DNA. The cAMP-CRP complex is involved in regulation of many genes at bacteria. The cAMP-CRP regulatory element represents, in some respects, a global regulatory network. The aim of this work was to study the structure and the mechanisms controlling the expression of CRP in Serratia marcescens. We have been get 5 different clones from Serratia which stimulated the cells to use maltose as a sole carbon source in E. coli TP2139. The crp gene clone, pCKB12, was confirmed by Southern hybridization with E. coli crp gene. The location of the crp gene was determined by constructing subclones carrying various portions of pCKB12. To investigate the potential role of CRP in E. cloi, lacZ fused plasmids were constructed and investigated the β-galactosidase activity of the fused plasmid. The Serratia marcescens cAMP receptor protein can substitute the E. coli CRP in transcriptional activation at the lacZ gene. These results suggest that Serratia marcescens cAMP receptor protein complex functions to regulate several promoters in E. coli.

      • KCI등재

        當歸의 種類에 따른 四物湯이 白鼠의 胸部大動脈 血管弛緩에 미치는 影響

        김형환,이주호,이제현,안덕균,박성규 대한본초학회 2001 大韓本草學會誌 Vol.16 No.2

        We have examined the relaxational reponse to the water extract of Angelica gigas Nakai (AG), A. sinensis(Oliv.) Diels (AS), A. acutiloba Kitagawa (AA), and Four-Substance Decoctions (AG-FSD, AS-FSD, AA-FSD) in isolated thoracic aorta from sprague dawley(SD) rat in the presence and absence of endothelium. Rat thoracic aorta was investigated in vessel segments suspended for isometric tension recording by polygraph. Responses to AG, AS, AA and AG-FSD, AS-FSD, AA-FSD were investigated in vessels precontracted with 5-hydroxytryptamine(5-HT) were compared in vasodilation effect. We found that the thoracic aorta segments responded to AG, AS, AA and AG-FSD, AS-FSD, AA-FSD with a dose-dependent vasodilation. The 5-hydroxytryptamine induced contraction at 10-4M were inhibited by 26.3%, 2.7%, 2.1% and 24.6%, 1.1%, 3.2% after addition of the 0.1 g/㎖ water extract of AG, AS, AA and AG-FSD, AS-FSD, AA-FSD. The 5-hydroxytrptamine induced contraction at 10^-4M with and without endothelium were inhibited by 24.6% and 6.9% after addition of the 0.1 g/㎖ water extract of AG-FSD. In conclusion, AG, AS, AA and AG-FSD, AS-FSD, AA-FSD-induced relaxation in the isolated rat thoracic aorta were composed of dose-dependent relaxation. AG-FSD has very potent vasodilation.

      • KCI등재

        지역·지구 경계부 대지의 건축기준 적용에 관한 연구 : 개발용량에 의한 총합개념을 중심으로

        최찬환,황용연,김주석 대한건축학회 2003 대한건축학회논문집 Vol.19 No.6

        The purpose of this study is to analyze a problem of the application of 'Korean building code' occurred by discord a zoning line and a lot line, and to find a way out of this problem. The application of 'Korean building code' is based on the development capacity which is decided by urban planning, and it is grounded that 'the total development capacity' should not be over-estimated for the public profit. In a lot, that is including several zones, we suppose to estimate the 'floor area ratio'&'building coverate ratio', which determine the lot's development capacity, by respective zone's area ratio.

      • 接觸安定法을 이용한 糞尿處理에 관한 硏究

        全鍾煥,李柱錫,蘇在喆,安松燁 圓光大學校 1987 論文集 Vol.21 No.2

        本 硏究에서는 연구책임자의 學述的 硏究經驗을 토대로 하여 보다 나은 處理工程을 다음과 같이 개발하였다. 旣存의 糞尿處理場 1次處理工程인 好氣性消化槽를 接觸槽와 安定槽로 분리하고 2次沈澱池에서 배출되는 返送슬러지를 全部 安定槽로 流入시켜 安定化시킨후 接觸槽로 보내는 接觸安定法의 工程으로 改造하고 流入糞尿 1對 稀釋水8의 比率로 糞尿를 流入시키며 實驗考察한 결과 97%이상의 處理效率을 얻었고 惡臭가 거의 발생하지 않은 쾌적한 環境이 되었다. In this study, the researchers found the better treatment process of nightsoil treatment plant on the basis of the responsible researcher's academic experience. The first treatment process, that is, aerobic digester of the existing nightsoil treatment plant is separated into the contact tank and the stabilization tank. And all the return sludge which discharge from the final sedimentation tank is folloed in the stabilization tank and is kept the stabilization situation there Afterwards, it is sent to the contact tank. The researchers remodeled as these contact stabilization processes and put in the contact tank at rate of inflow nightsoil one to diluted water eight. The results of above practical experiences showed the effective treatment above 95 percents and the comfortable environment with few foul ordor.

      • 다중 제어루프에 의한 자기부상용 DC-DC 전원장치에 관한 연구

        조주현,조정민,전기영,이상집,이승환,오봉환,이훈구,한경희 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

        The author present a modified multi-loop algorithm including feedforward for controlling a 55kW step down chopper in the power supply of Maglev. The control law for the duty cycle consists of three terms. The first is the feedforward term which compensates for variations in the input voltage. The second term consists of the difference between the slowly moving inductor current and output current. The third term consists of proportional and integral terms involving the perturbation in the output voltage. This perturvation is derived by subtracting the desired output voltage from the actual output voltage. The proportional and integral action stabilizes the system and minimizes output voltage error. In order to verify the validity of the proposed multi-loop controller, simulation study was tried using Matlab simulink.

      • 대용특성을 이용한 측정시스템 분석

        김주환,장무성,김상부 國立 昌原大學校 產業技術硏究院 2004 産技硏論文集 Vol.18 No.-

        Since 1996, many Korean companies such as Samsung, LG, Hyundai Motor Company, have adopted Six Sigma in order to get a competitive edge in the global marketplace. There are, however, many problems to be solved for successfully implementing Six Sigma in an organization. As is known, one of the key principles of Six Sigma is a data-based management or a decision making based on data. Therefore, Six Sigma requires more measurement than ever, which makes measurement system analysis more important. Usually, a gage R&R study is performed to identify the measurement system's variation relative to process variation. In the study, a gage R&R study is considered for the case where measuring a major quality characteristic accompanies a destructive testing. We suggest a gage R&R study using a screening variable that is highly correlated with the major quality characteristic and also can be measured by a nondestructive testing method. It is assumed that the major quality characteristic and a screening variable have a bivariate normal distribution. A simulation study on the proposed method is performed and we compare it with the conventional gage R&R study for a destructive testing.

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